Labshake search
Citations for GE Life Sciences :
1251 - 1300 of 2758 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... T cells were depleted by erythrocyte rosetting using sheep red blood cells and B cells were separated by Ficoll density gradient centrifugation as recommended by the manufacturer (GE Healthcare). The remaining erythrocytes were lysed in red blood cell lysis buffer (155 mM NH4Cl ...
-
bioRxiv - Immunology 2021Quote: ... Digested tissue was filtered through 100-μm cell strainers and colon and lung cells were further enriched by 40% Percoll (GE Healthcare) centrifugation ...
-
bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
-
bioRxiv - Cell Biology 2022Quote: ... The fluorescence levels of Cyclin B1 in HeLa cells treated with nocodazole were measured using the IN Cell Developer Toolbox software (GE Healthcare). After background subtraction ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were seeded at ∼ 7.93 × 108 total viable cells (around 7.93 × 105 viable cells/mL) in a chemically defined media (ActiPro media; GE Healthcare, USA). The cultures were maintained with setpoints for pH at 7.2±0.05 ...
-
bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
-
bioRxiv - Molecular Biology 2023Quote: ... The medium was changed one day after transfection and live cells were imaged on an IN Cell Analyzer 2200 (GE Healthcare) at 4x magnification before the plate was returned to the incubator and cells were allowed to expand ...
-
bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) or polymorphonuclear cells (PMNs) were isolated from whole blood by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare) or Polymorphprep (Axis-Shield) ...
-
bioRxiv - Microbiology 2020Quote: 3D structured illumination microscopy of fixed T cells cells was performed with a commercial Deltavision OMXv4.0 BLAZE microscope (GE Healthcare, Amersham, UK) using a 60x ...
-
bioRxiv - Cancer Biology 2020Quote: ... The mantle cell lymphoma cell line Maver-1 (#ACC 717, DSMZ Gmbh, Braunschweig, Germany) was cultured in HyClone™ RPMI 1640 medium (GE Healthcare) supplemented with 10% heat-inactivated FBS and 1% Penicillin/Streptomycin.
-
bioRxiv - Cancer Biology 2023Quote: ... which was determined by imaging of DAPI stained cells using the Incell 2200 or 6500 Cell Imaging Systems (GE Healthcare, Parramatta, Australia).
-
bioRxiv - Cell Biology 2020Quote: ... the membranes were incubated with horse-radish peroxidase-conjugated secondary antibody raised against rabbit or mouse (GE Healthcare) for 1 h at room temperature (25°C) ...
-
bioRxiv - Cell Biology 2019Quote: All blots were probed with an HRP-conjugated donkey anti-rabbit secondary antibody (GE Healthcare, catalog number NA934V) or HRP-conjugated donkey anti–mouse antibody (GE Healthcare ...
-
bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies were incubated for 1 hour at RT and signals were detected with ECL Select (GE Healthcare) and Odyssey Fc (Li-Cor) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Msh5 ChIP was performed using polyclonal Msh5 antibody (generated in rabbit) and Protein A Sepharose beads (GE Healthcare) on synchronized meiotic cultures as described in Krishnaprasad et al ...
-
bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
-
bioRxiv - Immunology 2021Quote: Purified recombinant antibodies were assessed by SDS-PAGE under nonreducing conditions using the Amersham Phastsystem™ (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
-
Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... Secondary antibodies used included anti-rabbit IgG-HRP (NA934V) and anti-mouse IgG-HRP (NA931V) from GE Healthcare. Chemicals used in this study included Ubiquitin vinyl sulfone (U-202) ...
-
bioRxiv - Microbiology 2019Quote: ... Anti-CFP polyclonal antibodies were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare) according to manufacturer instructions.
-
bioRxiv - Genetics 2019Quote: ... Primary antibodies were detected by HRP-conjugated anti-rabbit IgG (GE Healthcare NA934V, Lot 9636020; 1:10000 dilution) or HRP-conjugated anti-mouse IgG (GE Healthcare NA931V ...
-
bioRxiv - Neuroscience 2020Quote: ... 1h at room temperature with the secondary antibody and developed using an ECL prime solution (GE Healthcare, RPN2232V1).
-
bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies (goat anti-mouse IgG-HRP, DACO, Glostrup, Denmark and donkey anti-rabbit IgG-HRP, GE Healthcare) were diluted 1:5000 in 5% milk in TBS-T and incubated for 1 hr at room temperature ...
-
bioRxiv - Biophysics 2020Quote: ... the medium was loaded overnight onto a ~5 ml anti-CD9 AR40A746.2.3 affinity column (7.5 mg antibody/ml CNBr Activated Sepharose 4B beads; GE Healthcare) in line of an AKTA explorer (GE) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies were diluted in blocking solution at 1:1000 (Anti-rabbit IgG: GE Healthcare Cat#NA934, RRID:AB_772206) and 1:2000 (Anti-mouse IgG ...
-
bioRxiv - Immunology 2022Quote: IgG was purified by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences, 17040203) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
-
bioRxiv - Biochemistry 2022Quote: ... and then antibody-antigen complex was detected by ImageQuant LAS 500 (Cytiva) using enhanced chemiluminescence system (GE Healthcare). The diluted series of recombinant CAHS3 proteins (2.5 ...
-
bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies are HRP-conjugated anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 111-035-144 or GE Healthcare, #NA934), HRP-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by anti-rabbit-IgG and mouse-IgG antibodies coupled to HRP (1:2000, NA934, NA931, GE Healthcare).
-
bioRxiv - Cell Biology 2020Quote: ... The labeled antibody was separated from free Cy7 using a PD-mini G-25 desalting column (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS ...
-
bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with fluorescently conjugated secondary antibodies and revealed by a Typhoon Variable Mode Imager (GE Healthcare) or with HRP-conjugated secondary antibodies and ECL Prime Western Blotting System (#RPN2106 ...
-
bioRxiv - Cancer Biology 2020Quote: ... the membrane was incubated for 1 hour with horseradish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare, Piscataway, NJ). The target protein on the membrane was detected by Pierce™ ECL Western Blotting Substrate (Thermo Scientific ...
-
bioRxiv - Biophysics 2019Quote: ... The solubilized fraction was subjected to affinity purification by 1D4 antibody conjugated to CNBr-activated agarose (GE Healthcare). The resin was extensively washed and the protein eluted with wash buffer supplemented with 0.2 mg/ml 1D4 peptide ...
-
bioRxiv - Biochemistry 2020Quote: ... Antibodies used for ChIP experiments were purified with rProtein A Sepharose (PAS) beads (GE Healthcare, Cat#171279-01).
-
bioRxiv - Cancer Biology 2020Quote: ... The membranes were then incubated with anti-Mouse HRP-conjugated secondary antibody (1:5000 of NA931, GE Healthcare) for 1 hour at room temperature followed by three washes with PBST ...
-
bioRxiv - Microbiology 2021Quote: Antibody affinities were determined by SPR essentially as described44 using a Biacore 8K instrument (GE Healthcare, Chicago, IL). To measure antibody interaction affinity rather than avidity ...
-
bioRxiv - Neuroscience 2020Quote: ... the wells were washed five times and an anti-rabbit HRP conjugated detection antibody (GE Healthcare, Helsinki, Finland) was added at a 1:5000 dilution ...
-
bioRxiv - Genetics 2019Quote: ... Antibody signals were developed using ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038-560) and bands were quantified with Gel Doc system (Bio Rad ...
-
Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... specific for both primary antibodies and the signal was revealed by ECL Western Blotting Analysis System (GE Healthcare). Additional p-eIF4E ...
-
bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies used in Western blotting and IF were HRP sheep anti-mouse IgG (GE Life Sciences NA931V), goat anti-mouse IgG IRDye800CW (LiCoR 926-32210) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies were visualized with ECL plus substrate (Pierce, 32132) on a Typhoon FLA9500 (GE Healthcare Life Sciences) for co-IP blots and a ChemiDoc MP Imager (Bio-rad ...
-
bioRxiv - Cancer Biology 2021Quote: ... This was followed by the corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3,000; GE Healthcare, Freiburg, Germany). An electrochemiluminescence assay (GE Healthcare ...
-
bioRxiv - Biophysics 2020Quote: ... uncoupled dyes were separated from labelled antibodies by size exclusion chromatography (on Sephadex G25 PD10 columns GE Healthcare) followed by dialysis against PBS 1x overnight at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies bound to the PVDF membrane were then detected using enhanced chemiluminescence reagents (GE Healthcare, NJ); the chemiluminescence was digitally imaged and analyzed with the ChemiDocTM MP imaging system and associated software (Bio-Rad ...
-
bioRxiv - Developmental Biology 2020Quote: ... Flag primary antibody (monoclonal M2 mouse IgG1) and enhanced chemiluminescence assay (Amersham ECL Primer, GE Healthcare Life Science) were used for detection.
-
bioRxiv - Microbiology 2021Quote: ... The apparent binding affinity (KD) for individual antibody was calculated using BIAcore® 3000 Evaluation Software (GE Healthcare).
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... or HRP-conjugated anti-mouse IgG secondary antibody (GE Healthcare, diluted 1:4000 in PBS with 1% BSA). Cells were then incubated in o-phenylenediamine (OPD ...
-
bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies used for western blotting are as follows: α-mouse-HRP (1:1000) (GE Healthcare, Piscataway, NJ) for α-O-GlcNAc primary antibody ...
-
bioRxiv - Molecular Biology 2020Quote: ... followed by an incubation with horseradish peroxidase (HRP) secondary antibodies (Mouse IgG HRP Linked Whole Ab-GE Healthcare, #NA931 ...