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Citations for GE Life Sciences :
1251 - 1300 of 4203 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The diluted protein was applied to a heparin column (HiTrapHeparin HP, GE Healthcare), washed with 20 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 150 mM sodium chloride (HBS) and loaded onto protein G resin (GE Healthcare) equilibrated with HBS ...
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bioRxiv - Molecular Biology 2022Quote: ... The GST fusion proteins were purified with Glutathione Sepharose ™4B (GE Healthcare) according to the manufacturer’s protocol with Binding Buffer I [50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... through a 1 ml HiTrap Protein G HP column (GE Healthcare Life Sciences) previously equilibrated with Loading Buffer (0.02 M NaH2PO4 pH 7) ...
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bioRxiv - Immunology 2022Quote: ... The biotinylated proteins were further purified by gel filtration (S200, GE Life Sciences) in PBS buffer pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare), and the membrane was blocked with 5% non-fat dry milk for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred onto PVDF membranes (Amersham Hybond 0.45 µm, GE Healthcare). For immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... tagged proteins were affinity-purified with a HisTrap HP nickel column (GE Healthcare) or glutathione Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... Protein bands bound by antibodies were visualized using chemiluminescent detection reagent (GE Healthcare) on an iBright FL1500 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... Free dye was separated from labelled protein by Illustra NICK columns (GE Healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2019Quote: ... for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for GST-tagged proteins at 4 °C following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... for the MBP-tagged proteins or glutathione sepharose resin (GE Healthcare Life Sciences) for the GST-tagged proteins ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were further purified by gel filtration using superdex 75 column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were further purified using a MonoQ 10/100 column (GE Healthcare) and eluted with a KCl salt gradient from 100 mM to 600 mM ...
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bioRxiv - Plant Biology 2019Quote: ... The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare) was incubated with the Arp2/3 complex in pull-down buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pathology 2020Quote: Immunoblotting was performed after protein transfer onto Hybond-P membranes (GE Healthcare, RPN303F). After blocking membrane nonspecific binding sites in TBS (20 mM Tris ...
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bioRxiv - Biophysics 2019Quote: ... Commercial NP protein (H3N2) was covalently immobilized on CM5 chips (GE Healthcare, USA) and used as a ligand ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) using film or the ChemiDoc Touch Imaging System (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow, GE Healthcare) and the purity and integrity were checked by SDS–PAGE ...
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bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by anion exchange chromatography (SOURCE 15Q, GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... bead-coupled proteins were incubated with PreScission Protease (GE Healthcare, 27-0843-01) in reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... and 700 μg protein was dissolved in 300 μL rehydration solution (GE Healthcare) for 1 hour at RT ...
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bioRxiv - Biophysics 2020Quote: ... The protein was further purified using a HiTrap Q HP column (GE Healthcare) for CTD-L and a HiTrap SP HP column for CTD with linear gradients of 50 to 500 mM NaCl ...
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bioRxiv - Immunology 2019Quote: ... Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow, GE Healthcare).
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bioRxiv - Synthetic Biology 2020Quote: ... Each protein was purified using a 1 mL HisTrap HP column (GE Healthcare), washing with 10 mM Hepes-NaOH ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged proteins were immobilized on glutathione sepharose beads (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were visualized by ECL chemiluminescence method using Amersham imager 600 (GE healthcare) and densitometry of protein bands was performed with ImageJ software.
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bioRxiv - Molecular Biology 2021Quote: ... The GST-proteins were purified using Glutathione Sepharose 4B (GE Healthcare Life Sciences) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... soluble proteins were loaded onto a Ni-column (HisTrap FF Crude, GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The sera were purified on Hi-Trap Protein G HP columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Expressed protein in supernatants were purified over a HisTrap excel column (GE Healthcare) and eluted in 500 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... the overexpressed protein was purified sequentially using HiTrap TALON crude column (GE Healthcare) and Superdex S200 26/60 column (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were purified using a 1 ml HisTrap HP column (GE Healthcare) or 5 ml GSTrap HP column (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... The dialyzed protein extract was purified via a His-trap column (GE Healthcare) previously equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was loaded on a SP column (from GE healthcare Life sciences) and eluted by applying a NaCl gradient (from 0.15 to 1 M) ...
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bioRxiv - Cell Biology 2020Quote: ... The eluted protein was fractionated using a Superose10 gel filtration column (GE Healthcare). Protein concentrations were determined using a NanoDrop 1000 spectrophotometer (Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The GST-TTD fusion protein was purified using Glutathione sepharose 4B (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and the protein was bound to a HiTrap heparin HP column (GE Healthcare), before elution with a gradient from 335 mM to 1 M KCl ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 µL of Protein A Sepharose 4 FF (GE Healthcare Japan, Tokyo, Japan) was added and mixed with rotation at 4°C for 3 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Protein purification was performed using a 5 ml StrepTrap HP column (GE Healthcare) followed by SEC using a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were purified sequentially by Ni-NTA affinity chromatography (HisTrap FF, GE Healthcare), heparin chromatography (HiTrap Heparin HP ...
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bioRxiv - Biochemistry 2021Quote: ... the expressed proteins were first purified by Glutathione Sepharose 4B beads (GE Healthcare), and then further purified with a HiLoad 16/600 Superdex 200 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was separated using a second HisTrap HP column (GE Healthcare). His6-tag free Ape1 protein was then loaded onto a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Phosphorylated proteins were recovered by gel filtration on Superdex 200 Increase (GE Healthcare) in 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were initially purified using Ni Sepharose 6 Fast Flow resin (GE Healthcare). The protein tags were cleaved with His-tagged TEV-protease overnight at 4 °C while dialyzing against TEV cleavage buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Buffer exchanged protein was applied to a 5mL HiTrap QHP column (GE Healthcare,) using a 30% gradient of QB buffer (20mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated and loaded onto a Superose 6 column (GE Healthcare) pre-equilibrated in purification buffer and peak fractions were pooled and concentrated to 5-6 mg/mL.
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bioRxiv - Developmental Biology 2021Quote: ... were coupled overnight at 4°C to protein A Sepharose beads (GE Healthcare). Coupled beads were washed three times in EB buffer (50 mM Hepes ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were visualized by chemiluminescent detection (ImageQuant LAS 4000, GE Healthcare Life Sciences) of peroxidase substrate activity (SuperSignal West Femto Maximum Sensitivity Substrate ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on SDS– polyacrylamide gel were transferred onto a nitrocellulose membrane (GE Healthcare). The bands were visualized utilizing Enhanced Chemiluminescence (Pierce ...