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Citations for GE Life Sciences :
1251 - 1300 of 1353 citations for Recombinant Mouse Jam2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... followed by the incubation with anti-rabbit or anti-mouse IgG antibody conjugated to horseradish peroxidase (NA934V or NA931V, GE Healthcare). After reactivation with Chemi-Lumi One Ultra (nacalai tesque) ...
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bioRxiv - Neuroscience 2021Quote: ... cortices lysates were immunoprecipitated with anti-Bin1 or mouse IgG 16 h at 4 °C and then with 30 μl of protein G–Sepharose beads (GE Healthcare) for 3 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Protein–antibody complexes were detected with HRP-conjugated goat anti-mouse-IgG (Jackson Research Laboratories, Bar Harbor, ME) or donkey anti-rabbit-IgG (GE Healthcare) secondary antibodies for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and developed using an anti-rabbit HRP secondary antibody (1:5000, GE Lifesciences, NA931) and an anti-mouse HRP secondary antibody (1:10,000, GE Healthcare, NA935). Blots were imaged using an Amersham Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... Anti-mouse IgG HRP-linked Whole Ab Sheep or anti-rabbit IgG HRP-linked Whole Ab Donkey (GE Healthcare Bio-Sciences) were used as secondary Abs for immunoblotting.
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Microbiology 2020Quote: ... each well in the plate was further incubated with the HRP-sheep anti-mouse or anti-human secondary antibody (1:5000, GE Healthcare) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare). The signal was visualized in a Chemidoc MP imaging system (BIO-RAD).
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bioRxiv - Microbiology 2020Quote: ... Sequence verified human (clone IMAGE:5309813) and mouse (clone IMAGE:30060666) cDNAs for PDGFRβ were obtained from the Dharmacon Mammalian Gene Collection (GE Healthcare) for creation of surface-expressed GFP fusion receptor constructs ...
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bioRxiv - Cell Biology 2021Quote: ... The horseradish peroxidase-labelled secondary antibodies used were: anti-rabbit IgG (NA934V) and anti-mouse IgG (NA931) antibodies from GE healthcare.
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bioRxiv - Microbiology 2022Quote: ... Aldolase was detected using the above protocol with 1:10,000 mouse anti-aldolase antiserum (a kind gift from David Sullivan) and 1:2,500 sheep anti-mouse HRP-linked secondary antibodies (GE healthcare, NA931).
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bioRxiv - Immunology 2022Quote: ... ELISA plates were afterwards washed 3 times with PBST and 50 μL of anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (Cytiva, GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibody was horseradish peroxidase (HRP)-conjugated anti-mouse IgG or IgA and a chemiluminescence HRP was used as substrate (GE Healthcare). Apparent molecular weight of bands was determined using a standard curve generated from molecular weight markers (r2=0.9916 ...
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bioRxiv - Biophysics 2021Quote: ... Antibody binding was detected by luminescence using 1:2000 diluted HRP-linked anti-IgG antibodies (CST #7074 for rabbit and CST #7076 for mouse) as the secondary antibodies and ECL prime reagent (GE Healthcare). The luminescence intensities were measured using ImageJ software (NIH) ...
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bioRxiv - Immunology 2022Quote: ... then probed with anti-mouse or anti-rabbit IgG secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, cat: NA9310V and NA9340V) diluted in TBST at room temperature for one hour with shaking at 60 rpm ...
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bioRxiv - Neuroscience 2019Quote: ... The blots were exposed to a peroxidase-conjugated anti-mouse secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare, Piscataway, NJ). The resulting chemilluminescent signal was acquired with a Molecular Imager Chemidoc EQ system (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... the membranes were incubated for 2 h in Can Get Signal Solution 2 (Toyobo) with anti-rabbit or mouse IgG conjugated to horseradish peroxidase (GE Healthcare). After washing twice with TBST ...
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bioRxiv - Biophysics 2019Quote: ... Samples were subjected to Western blot using anti-1D4 monoclonal antibodies (University of British Columbia) and anti-mouse Horseradish peroxidase-conjugated antibodies (GE Healthcare). Protein bands were detected by enhanced chemiluminescence on X-ray film (ECL kit ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were washed with TBST three times before incubating with the corresponding secondary anti-mouse or anti-rabbit IgG antibody (GE Healthcare) in Can Get Signal Solution® 2 (TOYOBO) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were detected with HRP-conjugated goat anti-mouse or anti-rabbit IgG antibody and visualized with the ECL Western blotting substrate (GE Healthcare), according to the provided protocol ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies bound to the membrane were detected using horseradish peroxidase (HRP)-conjugated secondary antibodies against mouse or rabbit (GE Healthcare). Proteins were detected by chemiluminescence using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were then incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:2500, GE Healthcare Life Sciences) diluted in 2% non-fat milk prepared in PBS for 1 hour at RT followed by 3 washes with TBS containing 0.1% Tween-20 for 5 minutes each at room temperature ...
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bioRxiv - Physiology 2020Quote: ... lean mass and fat mass measurements were performed on each mouse by a PIXImus dual energy X-ray densitometer (GE Lunar, GE Healthcare). The PIXImus was calibrated daily with a mouse phantom provided by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... After incubating with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG as a secondary antibody at 1:2000 dilution (GE Healthcare.), protein bands were visualized by chemiluminescence and the image was captured by ImageQuant LAS 4000 mini (GE Healthcare).
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Cell Biology 2020Quote: ... antigen was CENP-AHCP-3(105-183)::6xHis) and detected using an HRP-conjugated secondary antibody (rabbit or mouse; GE Healthcare). For antibody production CENP-AHCP-3(105-183)::6xHis was expressed in E ...
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bioRxiv - Molecular Biology 2021Quote: ... Binding was detected by incubating the plates sequentially with either mouse anti-Histidine Tag antibody (MCA1396, Abd Serotec) followed by horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2000, NXA931, GE Healthcare) or Streptavidin-HRP (554066 ...
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bioRxiv - Cancer Biology 2021Quote: ... or HRP-conjugated anti-mouse (CST, 7076; 1:1000) IgG secondary antibodies and visualized using ECL Prime Western Blotting Detection Reagent (GE Healthcare) and ImageQuant LAS 4000 Mini (FUJIFILM) ...
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bioRxiv - Microbiology 2021Quote: ... were used for immunoblotting along with sheep anti-mouse-HRP NXA931V secondary antibody (GE Healthcare, 1:5,000 or 1:10,000, respectively). All yeast growth and Western blot data shown are representative of at least two biological replicates.
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bioRxiv - Microbiology 2022Quote: ... and bands visualized with (HRP)- coupled secondary antibody (1:5,000) (Cell Signalling, rabbit 7074S, mouse 7076S) on an ImageQuant LAS 4000 Mini (GE Life Sciences) with Luminata Forte Western HRP Substrate (Sigma WBLUF0500 ...
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bioRxiv - Physiology 2022Quote: ... lean mass and fat mass measurements were performed on each mouse by a PIXImus dual energy X-ray densitometer (GE Lunar, GE Healthcare). The PIXImus was calibrated daily with a mouse phantom provided by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary HRP-conjugated antibodies were purchased from Promega or GE Healthcare (anti-mouse Cat. No. W4021, Promega; anti-rabbit Cat. No. W4011, Promega; anti-rat Cat. No. NA935V, GE Healthcare). All secondary antibodies were diluted in 5% milk-PBS with 0.3% Tween 20 ...
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bioRxiv - Molecular Biology 2023Quote: ... These primary antibodies were detected using HRP conjugated anti-mouse and anti-rabbit IgGs and the ECL detection kit (all from GE Healthcare). Band intensities were quantified by densitometric analysis with Image Lab software (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V; HRP antirabbit NA934V, both GE Healthcare) and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
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bioRxiv - Immunology 2024Quote: ... then probed with anti-mouse or anti-rabbit IgG secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, cat: NA9310V and NA9340V) diluted in TBST at room temperature for one hour with shaking at 60 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies for Western blotting were HRP-linked sheep anti-mouse IgG and donkey anti-rabbit (both GE Healthcare; 1:5000) and HRP-linked mouse anti-rabbit ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then incubated with secondary ECL anti-rabbit or anti-mouse IgG HRP-linked antibody (GE Healthcare, Pittsburgh, PA) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Cell Biology 2023Quote: ... were used at a dilution of 1:10,000 and the HRP-linked sheep F(ab’)2 fragment to mouse IgG (GE Healthcare, NA9310) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by the appropriate horseradish peroxidase–conjugated anti-mouse (NA931V) or anti-rabbit (NA934V) secondary antibodies (GE healthcare, Chicago, IL, USA) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were probed with ECL Plex Cy3- or Cy5-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Cytiva) and scanned on an Amersham Typhoon 5 Phosphorimager (GE Healthcare).
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bioRxiv - Genetics 2023Quote: Knockdown of mouse Klf4 transcripts was performed with an ON-TARGETplus SMARTpool siRNA against mouse Klf4 (L-040001-01-005, 16600) (Dharmacon, GE Healthcare). This pool of four targeting RNAs targets the following sequences ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-rabbit horseradish peroxidase-linked IgG and anti-mouse IgG, horseradish peroxidase linked antibodies (NA944V and NA931, respectively) were from GE Healthcare. Polystyrene round-bottom tubes (5 mL ...
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bioRxiv - Physiology 2024Quote: ... Membranes were washed twice with TBS containing 0.1% Tween-20 and once with PBS containing 0.1% Tween-20 and then incubated with goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated IgG (GE Healthcare) diluted 1:10000 or 1:5000 ...
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bioRxiv - Immunology 2024Quote: ... An Fc capture chip was produced by amine coupling of anti-mouse IgG antibody to a CM5 chip using a commercial Fc Capture Kit (GE Healthcare). First ...
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bioRxiv - Molecular Biology 2024Quote: ... The membranes were washed with TBS-T three times for 10 minutes each and reacted with HRP-linked anti-rabbit or anti-mouse antibody (GE Healthcare) at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... a cDNA fragment encoding the C-terminal portion of mouse Ccrk (residues 289-346) was amplified by PCR and subcloned into the pGEX-4T-3 vector (GE Healthcare). The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3 ...