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Citations for GE Life Sciences :
1251 - 1300 of 1756 citations for IL 1R1 CD121a Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Whole-brain images will be acquired at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison HealthEmotions Research Institute (Department of Psychiatry) using a 3 Tesla GE MR scanner (Discovery MR750; GE Healthcare, Chicago, IL, USA). A high-resolution T1 image will be acquired with a fast spoiled gradient recalled echo sequence (8.2 ms TR ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad, Hercules, CA) for electrophoresis and transferred to a PVDF membrane (GE Healthcare Amersham, Chicago, IL). For cryba4 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The blots were developed using Radiance Plus chemiluminescent substrate (Azure Biosystems, Dublin, CA, USA) and the signals quantified using the ImageQuant LAS-4000 mini Imager (GE Healthcare, Chicago, IL, USA). Primary hepatocytes were used as control.
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bioRxiv - Developmental Biology 2022Quote: Unbiotinylated ectodomain transfections were collected and quantified by ELISA using nickel-coated microplates and pooled for purification using nickel-sepharose columns (HisTrap HP, GE Healthcare, Chicago, IL, USA) and an AKTAxpress chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... the target dsDNA from the PCR amplification was first concentrated by ethanol precipitation and then purified on an Akta Pure 150 FPLC instrument (GE Healthcare, Chicago, IL, USA) with a GE HiPrep 26/60 Sephacryl S-500 High Resolution size exclusion column and 0.3M NaCl running buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein were separated on 4-15 % Tris-glycine (TG) gels (#45101080003-2, SMOBIO, Hsinchu, Taiwan) and electro-transferred onto polyvinylidene fluoride (PVDF) membranes (#A16646282, GE Healthcare, Chicago, IL, USA) in TG buffer ...
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bioRxiv - Physiology 2019Quote: The optical density of cell suspensions was measured at a wavelength of 600 nm using an Ultrospec 10 spectrophotometer (GE Healthcare, Chicago, IL, USA). The cell dry weight (CDW ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were transferred to nitrocellulose membranes and visualized by enhanced chemiluminescence using ECL Prime Western Blotting Detection Reagent (#RPN2232, GE Healthcare; Chicago, IL, USA) and imager ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... for 1 h incubated with the appropriate antibodies and then visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Physiology 2021Quote: ... Membranes were again washed with 4 x 15 min TBS-T washes and a final 15 min TBS wash prior to band development with an ECL Prime Western Blot Detection Kit (GE Healthcare, Chicago, IL, USA) and imaged using a Chemidoc Imaging system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were separated on an SDS-15% polyacrylamide gel and then electrophoretically transferred onto the nitrocellulose membrane Amersham Protran 0.45 µm NC (GE Healthcare Lifesciences, Chicago, IL, USA). Membrane-immobilized proteins were probed with mouse anti-linker histone H1 monoclonal antibody clone AE-4 (Upstate Biotechnology ...
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bioRxiv - Genomics 2021Quote: ... as follows: The proteinase K-treated reaction was combined with 1 μL of Sera-Mag carboxylate-modified magnetic particles (GE Healthcare, Chicago, IL, USA.) and 72 μL of binding buffer (300 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cellular lysates were cleared by centrifugation and 300 μg of total protein were added to 30 μL of pre-equilibrated streptavidin-sepharose matrix (GE Healthcare, Chicago, IL, USA). Following 1 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and the size and purity of each antibody were determined by size exclusion chromatography using a Superdex 200 10/300 column (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Immunology 2021Quote: ... The blots were washed in TBS-T and incubated in secondary antibody in antibody buffer for 2 hours before washing again in TBS-T before visualising with ECL (GE Healthcare, Chicago, IL, USA) and imaging in an ALLIANCE 6.7 Chemiluminescence Imaging System (UVITEC ...
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bioRxiv - Biophysics 2022Quote: ... resolved in polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate (SDS-PAGE, 12% acrylamide) gels and transferred to PVDF membranes (GE Healthcare, Chicago, IL, USA) using standard procedures ...
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bioRxiv - Plant Biology 2022Quote: ... The fractions containing the protein were pooled and the imidazole removed on a PD-10 (sephadex G-25) column (GE Healthcare, Chicago, IL, USA), and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa ...
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bioRxiv - Microbiology 2022Quote: ... were mixed together at a molar ratio of 1:2 and loaded on a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare, Chicago, IL, USA) pre-equilibrated with 20mM Tris-HCl (8.0) ...
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bioRxiv - Microbiology 2023Quote: ... The gel filtration process was performed on an Äkta Pure 25 XK 16/70 column packed with 120 ml Superdex 200 (GE Healthcare, Chicago, IL, USA), and purified proteins were eluted with 1xPBS at 1 ml/min by collecting 2 ml per fraction ...
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bioRxiv - Plant Biology 2022Quote: ... 3 mL of the centrifuged supernatant was applied to a PD-10 column filled with Sephadex G-25 resin (GE Healthcare, Chicago, IL, USA) equilibrated with the homogenizing buffer ...
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bioRxiv - Microbiology 2022Quote: ... a 1.5 cm x 50 cm (bed volume 85 mL) was packed with Sephacryl™ S-1000 superfine resin (GE Healthcare, Chicago, IL, USA) [43] and equilibrated with two bed volumes of PBS ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Molecular Biology 2023Quote: ... Concentrated media was then subjected to size exclusion chromatography using Sepharose 4 fast flow column connected to the ÄKTA prime system (both GE Healthcare, Chicago, IL, USA) and eluted in PBS with a flow rate of 0.5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... The flow-through was collected and purified by ion-exchange chromatography on a HiTrap Q HP column (GE Healthcare Life Sciences, Chicago, IL, USA), using Buffer I1 (20 mM Tris ...
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bioRxiv - Genetics 2023Quote: ... Then these cells were repositioned under the DeltaVision microscope (OMXTM V4; GEhealthcare, Chicago, IL, USA) and the super-resolution images were processed with SoftWoRx (Applied Precision, Issaquah, WA, USA). And the distance between axial elements was measured using IMAGEJ 1.37a software.
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bioRxiv - Biochemistry 2023Quote: ... or 25 °C (ASK1 TBD-CRR and ASK1 CRR) for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
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bioRxiv - Immunology 2023Quote: ... and processed within 2 hours by dilution at 1:1 (v/v) ratio in phosphate-buffered saline solution (PBS) with layering on top of 1 volume of Ficoll-Paque Premium (GE Healthcare, Chicago, IL, USA) and separation by density centrifugation at 500× g for 30 minutes (20°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the flow-through sample containing the ASK1 TBD protein was purified by size-exclusion chromatography on a HiLoad 26/600 Superdex 75 pg column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM HEPES (pH 7.0) ...
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bioRxiv - Biochemistry 2023Quote: ... thawed ASK1 TBD-CRR-KD was subjected to size exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... ASK1 TBD was expressed at 25 °C for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The oligomeric state and purity were determined at 25°C by size-exclusion chromatography on a Superdex 200 10/300GL column (GE Healthcare Technologies, Chicago, IL).
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse IgG HRP (NA931V) (both from GE Healthcare) and anti-goat IgG HRP (HAF109 ...
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bioRxiv - Cell Biology 2020Quote: ... peroxidase-linked sheep α-mouse IgG (GE Healthcare, #NA931V) and AMDEX goat α-rabbit IgG (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Horseradish peroxidase conjugated Goat anti-Mouse IgG (GE Healthcare) diluted 1:5000 was used as secondary antibodies for the detection of Mouse IgGs.
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bioRxiv - Neuroscience 2021Quote: ... and sheep anti-mouse (NA931, GE Healthcare, 1:10000). The guinea-pig anti-KCTD16 antibody was raised against a synthetic peptide derived from mouse KCTD16 (aa7-23 ...
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bioRxiv - Molecular Biology 2020Quote: ... and HRP-linked anti-Mouse IgG (NA931, GE Healthcare) were used as secondary antibody ...
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bioRxiv - Genomics 2020Quote: ... HRP-conjugated anti-mouse (GE Healthcare NA931V, 1:10000) and HRP-conjugated anti-rabbit (Santa Cruz sc-2004 1:5000 ...
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bioRxiv - Microbiology 2019Quote: ... sheep anti-mouse HRP-linked IgG (GE Healthcare, UK) for Western immunoblotting and rat anti-mouse IgG H+L conjugated with FITC for FACs analysis (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... Anti-rabbit and anti-mouse secondary antibodies (GE Healthcare) were used in 2.5% milk-fat for one hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... followed by secondary HRP-conjugated anti-mouse (GE Healthcare) or anti-rabbit (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies were: α-Mouse-HRP (GE Healthcare), α-Rabbit-HRP (GE Healthcare) ...
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bioRxiv - Systems Biology 2020Quote: ... and ECL mouse HRP-linked IgG (GE Healthcare, NA931).
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bioRxiv - Cancer Biology 2020Quote: ... anti-mouse (GE Healthcare Cat# NA931, 1:5000 dilution), and then detected using an ECL SuperSignal West Femto y West Pico detection kit (Pierce).
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bioRxiv - Cell Biology 2022Quote: ... HPR-conjugated anti-rabbit or anti-mouse (GE Healthcare) was incubated with membranes ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse IgG HRP linked (1:7,500, NXA931V, GE Healthcare). We used ECL Western Blotting detection reagent (RPN2232 and RPN2209 ...
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bioRxiv - Neuroscience 2020Quote: ... A plasmid coding for mouse JNK3 cDNA (GE Healthcare) was used as a PCR template and the resultant product was subcloned into mammalian expression vector pRK5 downstream of a myc epitope tag ...
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bioRxiv - Neuroscience 2019Quote: ... and mouse Cux2 cDNA were obtained from GE Healthcare (Clone ID 5727802 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse or rat IgG was purchased from GE Healthcare.
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bioRxiv - Cell Biology 2020Quote: ... and HRP-anti mouse secondary (GE Healthcare; 1:5000). Western blots were developed on X-ray film using a SRX-101A table top film processor.