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Citations for GE Life Sciences :
1251 - 1300 of 1353 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Cancer Biology 2021Quote: ... or HRP-conjugated anti-mouse (CST, 7076; 1:1000) IgG secondary antibodies and visualized using ECL Prime Western Blotting Detection Reagent (GE Healthcare) and ImageQuant LAS 4000 Mini (FUJIFILM) ...
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bioRxiv - Immunology 2021Quote: ... All antibody formats were expressed using ExpiCHOTM Expression system (Thermoscientific, USA) and purified on HiTrap Protein-A column (GE healthcare, UK). The integrity and purity of the antibodies were analyzed using SDS-PAGE.
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bioRxiv - Immunology 2021Quote: The binding affinities of antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Genetics 2020Quote: 200 µg of antibodies R1 and R2 (against HSF2BP and MIDAP) were crosslinked to 100 ul of sepharose beads slurry (GE Healthcare). Testis extracts were prepared in 50mM Tris-HCl (pH8) ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
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bioRxiv - Developmental Biology 2022Quote: ... Secondary antibodies coupled to horseradish peroxidase were used for enhanced chemiluminescence signal detection with ECL Prime Western Blotting System (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...
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bioRxiv - Immunology 2022Quote: ... RPAPGST(GalNAc)APPAHG and RPAPGS(GalNAc)T(GalNAc)APPAHG with immobilized antibody 14A and 16A were determined by surface plasmon resonance on a Biacore T200 (GE Healthcare) instrument ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Molecular Biology 2022Quote: ... were used in immunoprecipitation experiments with the anti-Cdc45 rabbit antibodies and control rabbit IgG bound to Protein A Sepharose beads (GE Healthcare). Mixtures were incubated for a minimum of 3 hr (or overnight ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... except that total extracts were subjected to pull-down using monoclonal antibodies against Cherry bound to protein G-Sepharose 4 Fast Flow beads (GE Healthcare). The suspensions were end-over-end mixed on a rotating wheel for 2 h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... then probed with anti-mouse or anti-rabbit IgG secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, cat: NA9310V and NA9340V) diluted in TBST at room temperature for one hour with shaking at 60 rpm ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were visualized by incubation with the indicated antibodies and developed using the chemiluminescence function on an Amersham Imager 600 (GE Healthcare) or a Compact 2 developer (Protec) ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... Bound proteins were eluted by boiling in SDS–PAGE sample loading buffer and analyzed by western blot using an anti-GST antibody (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
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bioRxiv - Developmental Biology 2022Quote: ... was used as the secondary antibody. Protein was detected using the chemiluminescent substrate ECL (Cat. # 32209, Pierce) in an Amersham Imager 680 (GE Healthcare) detector.
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bioRxiv - Developmental Biology 2023Quote: ... Then it was incubated with a secondary antibody and detected using a Medical Film Processor (SRX-101A) or ImageQuant LAS 4000 (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were then incubated over night at 4 °C with appropriate antibodies (1ug/ml) and protein A Sepharose beads (GE Healthcare). After incubation samples were washed five times with lysis buffer and resuspended in 1X Laemmli sample buffer (Bio-Rad cat ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by the appropriate horseradish peroxidase–conjugated anti-mouse (NA931V) or anti-rabbit (NA934V) secondary antibodies (GE healthcare, Chicago, IL, USA) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3x for 10 min with TBST and subsequently incubated for 1 hr at room temperature with appropriate horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) diluted (1:10,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were probed with ECL Plex Cy3- or Cy5-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Cytiva) and scanned on an Amersham Typhoon 5 Phosphorimager (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... HA-UL47-containing protein complexes were immunoprecipitated by incubating extracts with HA-specific antibodies and protein G-coupled sepharose beads (4 Fast Flow, GE Healthcare). The sepharose-bound proteins were analyzed by standard immunoblotting for the presence of HA-UL47 ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-rabbit horseradish peroxidase-linked IgG and anti-mouse IgG, horseradish peroxidase linked antibodies (NA944V and NA931, respectively) were from GE Healthcare. Polystyrene round-bottom tubes (5 mL ...
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bioRxiv - Immunology 2023Quote: The binding kinetics and affinity of monoclonal antibodies to the SARS-CoV-2 spike trimer were analyzed by SPR (Biacore T100, GE Healthcare). Specifically ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were detected with host-specific secondary antibodies and developed with a chemiluminescent substrate (GE Healthcare Amersham, Piscataway, NJ, USA). After stripping ...
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bioRxiv - Systems Biology 2024Quote: ... Antibody bindings were visualized with ECL Prime Western Blotting Detection Reagent kit (Cytiva) and imaged by using ImageQuant LAS500 (GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... An Fc capture chip was produced by amine coupling of anti-mouse IgG antibody to a CM5 chip using a commercial Fc Capture Kit (GE Healthcare). First ...
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bioRxiv - Molecular Biology 2024Quote: ... and horseradish peroxidase-conjugated anti-rat (NA935V [1:1000]) and anti-mouse (NA931V [1:1000]) IgG antibodies (GE Healthcare, IL, USA). Signals were detected using WesternSure Premium Chemiluminescent Substrate (#926-95010 ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was performed with the ChemiDoc-It 600 Imaging System (UVP, UK) using horseradish peroxidase-linked anti-rabbit and anti-mouse secondary antibodies (GE Healthcare, Austria) and ECL western blotting reagent (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Molecular Biology 2020Quote: ... with Tris-buffered saline/Tween-20 was followed by incubation for 1 h at room temperature with the appropriate secondary antibody (Anti-mouse IgG horseradish peroxidase cat# NA931V from GE Healthcare, Uk). Western blots were developed using the ECL Prime western blotting detection Reagent (RPN 2232 ...
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bioRxiv - Immunology 2022Quote: IgG was purified from cell culture media or plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Neuroscience 2020Quote: ... and anti-Cav2.3 (ACC-006, Alomone Labs) in combination with peroxidase-coupled secondary donkey anti-rabbit antibodies (NA934, GE Healthcare, 1:10000).
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: donkey anti-rabbit IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK) and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Postnuclear supernatants were incubated with 3 μg of antibodies coupled to protein A– or protein G–Sepharose beads (GE Healthcare, Solingen, Germany) for 4 h at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... The conjugated antibody was purified by SEC (Superdex 200 increase 10/300 GL, GE Healthcare, solvent: PBS, flow rate = 0.6 mL/min) to afford an antibody–linker conjugate [1.91 mg ...
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bioRxiv - Bioengineering 2021Quote: ... Slides were then washed twice with TBST (TBS with 0.05 % wt/v Tween 20) before incubating with the appropriate biotinylated secondary antibodies (GE Healthcare, 1:500) for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Microbiology 2021Quote: ... then incubated with secondary antibodies (horseradish peroxidase-conjugated anti-mouse IgG, GE Healthcare; or horseradish peroxidase-conjugated anti-rabbit IgG, GE Healthcare). Membrane was washed with PBST20 and imaged by chemiluminescence using ImageQuant LAS-4000 mini (GE Healthcare) ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: donkey anti-rabbit IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK) and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were then incubated for 1 h with a secondary antibody (goat anti-rabbit horseradish peroxidase-linked IgG [NA934V GE Healthcare] or sheep anti-mouse horseradish peroxidase-linked IgG [NA931V GE Healthcare]) and revealed with the Pierce ECL 2 Western Blotting Substrate (Thermo Fischer) ...
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bioRxiv - Immunology 2022Quote: ... Human IgG and IgA antibodies were purified from donors’ sera by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) and peptide M-coupled agarose beads (Invivogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were detected using horseradish peroxidase-conjugated secondary antibodies and detected using ECL (RPN2106) or ECL-select (RPN2235) reagents (Amersham, GE Healthcare, UK) and a cooled digital camera (LC33000 ...