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Citations for GE Life Sciences :
1151 - 1200 of 1839 citations for Upstream stimulatory factor 2 USF2 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were purified from hybridoma supernatant using HiTrap Protein G columns (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The secondary antibody ECL anti-mouse IgG (GE Healthcare Life Sciences, Buckinghamshire, UK), diluted 1:5000 in TBS buffer containing 5% milk powder was applied to the membrane for 90 minutes at room-temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The secondary antibody ECL anti-mouse IgG (GE Healthcare Life Sciences, Buckinghamshire, UK), diluted 1:5000 in TBST containing 5% milk powder was applied to the membrane for 90 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... a fluorescent Cy5-conjugated goat anti rabbit secondary antibody (GE Healthcare; 1:5,000) was used and the fluorescent signal acquired with a Typhoon fluorescent scanner (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... along with corresponding species IgG secondary antibodies (1:5000, GE Healthcare, Fisher Scientific) were utilised ...
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bioRxiv - Cancer Biology 2023Quote: ... The antigen-antibody complexes were visualized using an enhanced chemiluminescence system (GE Healthcare) and captured by Azure 500 system (Azure Biosystems).
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bioRxiv - Cell Biology 2023Quote: ... Signals were developed with horseradish peroxidase-conjugated secondary antibodies (GE Healthcare Bio-sciences) or peroxidase-conjugated primary antibodies using Western Lightning Plus-ECL (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: ... The antibodies were cross-linked to Protein G Mag Sepharose Xtra (GE Healthcare) according to the manufacturer’s instructions before immunoprecipitations ...
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bioRxiv - Microbiology 2024Quote: ... Bound antibodies reacted with Amersham ECL Prime (GE Healthcare Life Science, PA, USA) and were detected using ImageQuant LAS 4000 mini (GE Healthcare Life Science) ...
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bioRxiv - Bioengineering 2024Quote: ... or G12p4 antibodies were immobilized on a CM5 sensor chip (GE Healthcare Technologies) with an amine-coupling kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Protein bands reacting with antibodies were visualized using chemiluminescent detection reagent (GE Healthcare) on an iBright FL1500 (ThermoFisher Scientific).
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bioRxiv - Biophysics 2021Quote: ... Polyacrylamide gels were polymerized between two glass coverslips treated with 2% dimethyldichlorosilane (Plus One Repel Silane, GE Healthcare). For 0.6 kPa gels ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT at a flow rate of 2 ml/min using an Akta purifier system (GE Healthcare), eluting at approximately 100 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... Cell-free supernatant was applied to the column at 2 mL/min using an AKTA Pure (GE Healthcare), washed ...
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bioRxiv - Biochemistry 2020Quote: ... were subjected to the short column with 2 ml Sephacryl S-500 resin (GE Healthcare Life Sciences, IL) equilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... dried for 2 h at 65°C and radiolabelled products visualised using a Typhoon FLA-700 (GE Healthcare) following exposure to a phosphorimaging plate for 24-72 h.
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bioRxiv - Microbiology 2020Quote: ... concentrated to 2 mg/ml by spin filtration and then run on a Superdex 200 column (GE Healthcare). MALS was conducted using a MiniDAWN and Optilab system (Wyatt Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... the sample was loaded at 2 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) equilibrated in Dynein-lysis buffer and incubated for 3-4 h with rotation along the long axis of the tube ...
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bioRxiv - Biophysics 2022Quote: ... were further subjected to Ni-NTA purification by mixing with 2 mL of Ni-Sepharose resin (GE Healthcare) for 4 hrs and the bound proteins were eluted with a gradient of 100-500 mM imidazole in 10 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... were further subjected to Ni-NTA purification by mixing with 2 mL of Ni-Sepharose resin (GE Healthcare) for 4 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM DTE (IbpA) or applied to a HiLoad™ 16/600 Superdex™ 200pg column (GE Healthcare) in 20 mM Hepes pH 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: For mass spectrometry the beads were incubated with 150 µL the elution buffer (GE Healthcare, 2-1042-025) for 10 min at 4 °C while rotating ...
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bioRxiv - Molecular Biology 2022Quote: ... 93-207 Bcl-2) with a C-terminal 6His tag and purified using Ni2+ IMAC (GE Healthcare, Fastflow). Similarly ...
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bioRxiv - Immunology 2022Quote: The binding kinetics of nanobodies to SARS-CoV-2 RBD were analyzed using SPR (Biacore 8K; GE Healthcare). The nanobodies were captured by the ProA sensor chip and serial dilutions of SARS-CoV-2 RBD were then flowed through at a rate of 30 μL/min in PBS buffer (with 0.05% Tween20 ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL Glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2019Quote: ... proteins in 2% SDS were bound to Paramagnetic beads (SeraMag Speed beads, GE Healthcare, CAT#45152105050250, CAT#651521050502) on filter plates (Multiscreen ...
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bioRxiv - Synthetic Biology 2020Quote: ... the plate was blocked with 200 μl of a 2% (w/v) ECL Advance Blocking Reagent (GE Healthcare) solution in PBS-T (PBS supplemented with 0.1% (v/v ...
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bioRxiv - Genomics 2021Quote: ... 2 mM MgCl2) and then loaded onto two tandem 5 mL heparin HP pre-packed columns (GE Healthcare). Sen1 was eluted using a linear gradient from 20% to 100% of buffer B2 containing 50 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2020Quote: ... The NEC220 and NEC-CBM constructs were passed over 2 x 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange as previously described ...
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bioRxiv - Synthetic Biology 2022Quote: ... for 1-2 minutes in darkness then exposed and imaged using an Amersham™ Imager 600 (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed with ECL 2 western blot substrate and scanned with an ImageQuant LAS 4000 (GE Healthcare), and signals analyzed with ImageQuant TL 7.0 software (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... Cell-free supernatant was applied to the column at 2 mL/min using an AKTA Pure (GE Healthcare), washed ...
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bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (1×PBS ...
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bioRxiv - Biophysics 2024Quote: ... a 2-elution step Ni2+-affinity chromatography was carried out using a Histrap HP 5 ml (GE Healthcare) on an FPLC apparatus (ÄKTA Pure ...
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bioRxiv - Cell Biology 2019Quote: ... Bands were visualized by horseradish-peroxidase-conjugated sheep anti-mouse IgG antibodies (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse or anti-rat secondary antibodies (1:4000 in blocking buffer, GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Antibody-associated DNA:RNA hybrids were then captured on protein G Sepharose beads (GE Healthcare), washed and purified according to standard ChIP procedures ...
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bioRxiv - Cancer Biology 2019Quote: ... The clicked antibodies were purified by SEC (Superdex 200, 10×300 GL, GE Healthcare) at a flow rate of 0.5ml/min in PBS using a GE AKTAPurifier ...
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bioRxiv - Biochemistry 2019Quote: ... and coverslips were incubated with 1:100 primary anti-bromodeoxyuridine antibody (RPN202; GE Healthcare) in the dark for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... and IgG anti-rabbit horseradish peroxidase–conjugated secondary antibody (1:10,000; GE Healthcare UK). For whole cerebellum and cerebellar slices ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibody binding was followed by anti-mouse (GE Healthcare, catalog # NXA931V, lot # 17041890) or anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... we used a peroxidase-labeled anti-rabbit antibody (NIF824, GE Healthcare; 1:5000 dilution). Immunodetection was performed by incubating the membranes in the Western BLoT Quant HRP Substrate (Takara ...
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bioRxiv - Microbiology 2019Quote: ... IgG antibodies were purified from the supernatant using a Protein G column (GE Healthcare). The subclass of each IgG antibody was determined using a human IgG subclass ELISA kit (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... anti-rhodopsin antibody (1D4) to CNBr-activated Sepharose 4B beads (GE Healthcare Bio-Sciences) according to manufacturer’s instructions (Molday and Molday ...
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bioRxiv - Plant Biology 2022Quote: ... Immunoblot detection was performed with specific antibodies using the enhanced chemiluminescence system (GE Healthcare). Signals were detected with the G:box Chemi Imaging System (Syngene) ...
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bioRxiv - Plant Biology 2021Quote: ... chromatin-antibody complexes were precipitated with anti-IgG paramagnetic beads (GE Healthcare, Uppsala, Sweden). After six washing steps ...
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bioRxiv - Microbiology 2020Quote: ... incubation with primary/secondary antibodies and detection using Amersham ECL Plus reagents (GE Healthcare) were done following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Horseradish peroxidase-conjugated secondary antibodies were detected by enhanced chemiluminescence (GE Healthcare, cat # RPN2236) and imaged using a Chemidoc imager (Bio-Rad).