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Citations for GE Life Sciences :
1151 - 1200 of 2167 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... was purified by 1 M NaCl containing Tris buffer using AKTATM Pure protein purification system with Hi-Trap Q-FF anion exchange column (GE healthcare). 0.3% H2O2 was added to the purified proteins ...
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bioRxiv - Pathology 2021Quote: ... and 3500 V for 28 kVh (linear) with a Hoefer™ IEF 100 (M/s. GE Healthcare Life Sciences, Amersham, UK) at 20 °C ...
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bioRxiv - Microbiology 2020Quote: ... supernatants containing lentiviral particles were filtered (0.45 μm) and concentrated to 200 μl using Vivaspin filters using Vivaspin filters with a 100 kDa molecular weight cutoff (GE Healthcare). Subsequently ...
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bioRxiv - Biochemistry 2022Quote: ... were solubilised in TK buffer supplemented with 6 M urea before loading onto a 5 ml HisTrap HP column (GE Healthcare). The column was first washed with TK + 6 M urea followed by TK + 6 M Urea + 50 mM imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... (G4C2)4 RNAs biotinylated at the 5′ end in 10 mM HEPES pH 6.8 and 500 mM MCl (M = K, Na, or Li) was injected over the streptavidin-coated surface of a sensor chip SA (GE Healthcare). The amount of immobilized RNA was as follows ...
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The urea concentration was set to 6 M and the sample was applied to a 5 mL HiTrap QFF anion exchange column (GE healthcare) and was eluted using a 30 CV long gradient from low salt buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... HBS-P+ buffer (10 mM HEPES pH 7.4, 0.15 M NaCl, 0.05% v/v P20 surfactant, (GE Healthcare, Marlborough, MA, USA) was used as the running buffer ...
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bioRxiv - Microbiology 2023Quote: ... M-003854-01-0005) and a “NonTargeting” (SMARTpool siGENOME NonTargeting, D-001210-04-20) control siRNAs were obtained from GE Healthcare Dharmacon (Lafayette ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0, 0.15 M NaCl) using a HiPrep 26/10 column (GE Healthcare Life Sciences). The cleavage reaction was initiated by adding 6×His-tagged SUMO protease to a final concentration of ∼8 μg/mL to the protein solution ...
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bioRxiv - Plant Biology 2024Quote: ... gel containing 7 M urea and buffered with 0.5 X TBE using an SE600 standard dual-cooled gel electrophoresis unit (GE Healthcare, USA) until the bromophenol blue dye reached the bottom of the gel ...
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bioRxiv - Biochemistry 2022Quote: ... both MSL8N and MSL9N were placed in a high salt storage buffer (1 M NaCl, 200 mM Sodium Phosphate) using gravity PD-10 desalting columns (GE Healthcare). The protein was labeled with Alexa Fluor 647 NHS (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... of the extension reaction were separated by electrophoresis on a denaturing PAGE (8 M urea, 1× TBE, 15 % acrylamide) and then imaged (Typhoon 9500 imager; GE Healthcare) and the images were quantified using Image Lab software (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... and the soluble His6-M protein was purified on a Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 M NaCl and 30 mM imidazole and captured with Glutathione-Sepharose 4B beads as indicated by the manufacturer (GE Healthcare). The elution fractions were pooled and the buffer was exchanged to phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... Extension cDNA products were analyzed on 6% acrylamide 8[M urea-sequencing gels and visualized by Typhoon FLA 7000 phosphorimager (GE Healthcare). The four samples of the Sanger sequencing for dnaN were loaded to the same gel and served as a reference.
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bioRxiv - Biophysics 2024Quote: ... AF-1 proteins were heated in a hot water bath at 80 °C for 15 m to denature any additional contaminating proteins and centrifuged at 4000 rpm for 15 m before concentration and purification by anion exchange chromatography through a HiTrap Q HP column (GE healthcare) using a high salt buffer to elute protein from the column (20 mM phosphate pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... except 150 mM NaCl was used throughout the purification with a gradient of 0.15–0.95 M NaCl over 40 column volumes after a 1 column volume wash with 0.15 M NaCl on the MonoS cation exchange column (GE Life Sciences).
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bioRxiv - Biophysics 2020Quote: ... which contains DnaK protein was purified first with ion exchange chromatography using 0 to 1 M NaCl in a HiTrap column (HiTrap, GE Healthcare, Germany). Primary fraction of DnaK was eluted in 150 mM NaCl which was further purified using size exclusion chromatography using a superdex 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... bacterial-expressed GST and GST-α-Taxilin-M (expessed in Escherichia coli strain BL21) were bound to glutathione-Sepharose 4B beads (GE Healthcare) and incubated with bacterial-expressed MBP-γ-Taxilin-M or 293T cell expressed and purified 3×FLAG-CEP170 at 4 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed using an M-110S Microfluidizer (Microfluidics) and proteins were purified using glutathione chromatography according to the manufacturer’s instructions (GE Healthcare Life Sciences). To confirm the size and purity of purified recombinant protein ...
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bioRxiv - Biochemistry 2023Quote: ... The synthesized DNA products were separated on denaturing 30% PAGE with 7 M urea in 1xTBE and visualized on a Typhoon 9400 (GE Healthcare, USA).
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bioRxiv - Biochemistry 2024Quote: ... 30μM of His-crIFT172968-C or 30μM His-TEV protease were immobilized on 50μL of TALON beads (GE healthcare #28-9574-99) by incubation at 4°C for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... The gels were transferred for 2 hours onto nitrocellulose membranes (GE Healthcare Biosciences, Pittsburgh, PA). Detection was performed by diluting primary antibodies in TBS supplemented with 0.1% Tween and either 5% BSA or 5% non-fat dry milk ...
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bioRxiv - Developmental Biology 2021Quote: ... and proteins were electrotransferred to nitrocellulose membranes (Amersham Hybond-ECL, GE Healthcare, Chicago, IL, USA). The membranes were incubated overnight at 4°C with primary antibody (Table S2 ...
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bioRxiv - Molecular Biology 2020Quote: RNAs were electro-transferred from urea-PAGE gels to Hybond XL nylon membrane (GE Healthcare) at 12 V over 12 h in 0.5X TBE for Northern hybridisation ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments were transferred to a nylon membrane (Hybond™-N+) (GE Healthcare, Dornstadt, Germany) by capillary blotting ...
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bioRxiv - Molecular Biology 2021Quote: ... membranes were scanned on an Image Quant LAS 4000 (GE Healthcare, Little Chalfont, Buckinghamshire, UK) imaging station.
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bioRxiv - Physiology 2021Quote: ... The protein solution was then dropped on a nitrocellulose membrane (GE Healthcare Protran® BA85) to form 2 μL and 5 μL dots for each concentration group ...
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bioRxiv - Neuroscience 2022Quote: ... 20% MetOH) to a nitrocellulose membrane (Amersham™ Protran™ 0,45μm NC, GE Healthcare Lifescience). Quality of transfer was checked by Ponceau-S (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed in TBST before using the ECL Western Blotting Detection Reagent (GE Healthcare). The chemiluminescence signal was detected using the Amersham Imager 600 (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... Pieces of the retina were flattened on a filter membrane (Whatman, GE Healthcare Life Sciences) and placed on a poly-L-lysine (0.1% ...
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bioRxiv - Molecular Biology 2020Quote: ... gDNA on the gel was vacuum transferred onto a hybond nylon membrane (GE Healthcare GERPN303B) with 10XSSC (1.5M NaCl 0.17M NaCitrate ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were visualized using the chemiluminescent substrate of an ECL kit (GE Healthcare – RPN2232). Images were captured on ImageQuant LAS 4000 (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... the membrane was developed using enhanced chemiluminescence (ECL Prime Western Blotting detection reagent, GE Healthcare) in an ImageQuant LAS 4000 (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction mix was then transferred to the positively charged nylon transfer membrane (GE Healthcare) and washed extensively with 50 mM sodium phosphate buffer ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... pH 7.5 and filtered through a 0.22µm polyether-sulfone membrane (GE Healthcare, Chicago, IL, USA) to remove any aggregates ...
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bioRxiv - Neuroscience 2021Quote: ... and resolved by denaturing polyacrylamide electrophoresis before electrophoretic transfer to Nytran Plus membranes (GE Healthcare) and hybridization with [32P]-end labeled oligonucleotide probes at 42°C or direct staining ...
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bioRxiv - Cell Biology 2021Quote: ... Total protein was separated with SDS-PAGE gels and transferred to PVDF membrane (GE Healthcare). Membrane were blocked with 5% BSA in TBST for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred on Amersham Protran Premium 0.2 μm nitrocellulose membranes (GE Healthcare, Solingen, Germany) a Bicine/Bis-Tris transfer buffer (25 mM Bicine ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was washed and incubated with Amersham ECL Western Blotting Detection Reagent (GE Healthcare) and developed in the dark ...
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bioRxiv - Plant Biology 2021Quote: ... the proteins were transferred to the nitrocellulose membrane (GE Healthcare Life Sciences, Chicago, IL, USA), blocked with 3% BSA for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was incubated with a secondary anti-Rabbit horseradish peroxidase conjugated antibody (GE Healthcare) for 45 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... transferred to nitrocellulose membranes and radioactivity was detected in a Typhoon FLA950 apparatus (GE Healthcare). Later ...
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bioRxiv - Plant Biology 2020Quote: The blot was semi-dry transferred to Amersham Hybond™-N+ nylon membrane (GE Healthcare) and cross-linked under 0.12 J/cm2 UV irradiation ...
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bioRxiv - Neuroscience 2020Quote: ... containing a cellulose acetate membrane with pores with a 0.2 μm diameter (Whatman, GE Healthcare) as described in (Torres et al. ...
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bioRxiv - Plant Biology 2021Quote: ... gels were bolted onto a positively charged nylon membrane (Amersham, now GE Healthcare, http://www.gelifesciences.com). The DNA was linked using a UV light cross-linker instrument equipped with 254-nm bulbs for 0.8 min exposure.
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bioRxiv - Genomics 2021Quote: ... Electro-transfer was performed in TBE overnight onto a Hybond N+ membrane (GE Healthcare, RPN303B). Gene-specific probes were generated by random priming in the presence of ATP [α32P] using the Prime-It II Random Primer Labeling Kit (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... Fixed VLPs were filtered onto 0.02 μm filter membranes in triplicate (Anodisc, GE Healthcare, USA) and were stained with SYBR-Gold (2.5X concentration) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to Amersham Protran nitrocellulose membranes (0.45 µm pore size, GE Life Sciences) using a Trans-Blot Turbo transfer system (Bio-Rad ...