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Citations for GE Life Sciences :
1151 - 1200 of 1499 citations for Anti NEK9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... uncoupled dyes were separated from labelled antibodies by size exclusion chromatography (on Sephadex G25 PD10 columns GE Healthcare) followed by dialysis against PBS 1x overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies bound to the PVDF membrane were then detected using enhanced chemiluminescence reagents (GE Healthcare, NJ); the chemiluminescence was digitally imaged and analyzed with the ChemiDocTM MP imaging system and associated software (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatant using a HiTrap protein A column (Cytiva, GE Healthcare). IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva ...
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bioRxiv - Developmental Biology 2020Quote: ... Flag primary antibody (monoclonal M2 mouse IgG1) and enhanced chemiluminescence assay (Amersham ECL Primer, GE Healthcare Life Science) were used for detection.
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bioRxiv - Microbiology 2021Quote: ... The apparent binding affinity (KD) for individual antibody was calculated using BIAcore® 3000 Evaluation Software (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies used for western blotting are as follows: α-mouse-HRP (1:1000) (GE Healthcare, Piscataway, NJ) for α-O-GlcNAc primary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by an incubation with horseradish peroxidase (HRP) secondary antibodies (Mouse IgG HRP Linked Whole Ab-GE Healthcare, #NA931 ...
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bioRxiv - Molecular Biology 2022Quote: ... Signals were visualized with chemiluminescence (34095, Pierce) with a secondary antibody conjugated to Horseradish peroxidase (NXA931V, GE Healthcare). The chemiluminescence signal was obtained by the FUJI Medical X-ray film (4741019289 ...
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bioRxiv - Cell Biology 2023Quote: ... detection of antibodies was detected using the Amersham ECL Western Blotting Prime Blocking and Blotting Reagents (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... Blots were then probed with horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, GE Healthcare, NA934, RRID: AB_772206) for 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Antibody purification from cell supernatants was done in two steps using the ÄKTA Avant purification system (GE Healthcare). The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe ...
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bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... which was probed with a horseradish peroxidase-coupled secondary antibody (donkey-α-rabbit IgG, GE HealthCare, 1:400). Subsequent staining was performed using 3-amino-9-ethylcarbazol ...
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bioRxiv - Genetics 2024Quote: ... the membranes were incubated with a horseradish peroxidase-conjugated against the appropriate species antibody (1:10,000; GE Healthcare) diluted in the blocking buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by incubation with horseradish peroxidase-conjugated donkey anti-rabbit IgG (GE Healthcare). The blots were developed using the ECL system according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2020Quote: ... secondary stain was peroxidase sheep anti-mouse-HRP (1:5000) (45001275, GE Healthcare). TrueBlue Peroxidase Substrate (50-647-28 ...
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bioRxiv - Molecular Biology 2020Quote: ... and then incubated with HRP-conjugated donkey anti-rabbit IgG (GE Healthcare, NA934), diluted 1 ...
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bioRxiv - Genetics 2019Quote: ... and then with horseradish peroxidase-linked donkey anti-rabbit IgG (NA934, GE Healthcare). Blots were stripped and reprobed with rabbit anti-histone H3 antibodes (1:5,000 dilution ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were incubated with anti-rabbit IgG-HRP (GE Healthcare, NA934V, 1:2000) in TBST for 2 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and 100 µl peroxidase-conjugated goat anti-mouse immunoglobulin G (IgG) (GE Healthcare), IgG1 ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-rabbit (1:6,000-12,000; GE Healthcare – Life Sciences, ca. no. NA934) secondary antibodies conjugated with HRP and ECL Prime detection reagents were acquired from GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... polyclonal SagA serum and HRP conjugated anti-Rabbit IgG (GE Healthcare, NA 934V) served as primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2019Quote: ... probed with 1:8,000 of donkey anti-rabbit IgG (GE Healthcare, Piscataway, NJ) or sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... horseradish peroxidase (HRP)-conjugated IgG F(ab)2 donkey anti-rabbit (GE Healthcare), and HRP-conjugated affinity-purified goat anti-mouse IgG (Jackson).
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bioRxiv - Neuroscience 2020Quote: ... either sheep anti-mouse-HRP (1:5000, NA931V, GE Healthcare, Chicago, IL, USA) or donkey anti-rabbit-HRP (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... or donkey anti-rabbit-HRP (1:5000, NA934V, GE Healthcare, Chicago, IL, USA) diluted in 1x TBST and incubated for 1 h at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated with HPR-conjugated anti-mouse (GE Healthcare, Buckinghamshire, England) or anti-rabbit (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... for 1h at room temperature (GE Healthcare, NA934V, donkey anti-rabbit, 1:1000) a 5 min incubation with chemiluminescent HRP substrate (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with donkey anti-rabbit IgG-HRP (1:10,000, GE Healthcare) or sheep anti-mouse IgG-HRP (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... then reacted with goat anti-rabbit immunoglobulin G conjugated to HRP (GE Healthcare) at 1:10,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... followed by reaction with anti-rabbit immunoglobulin G conjugated to HRP (GE Healthcare) at 1:10,000 dilution and visualized by ECL ...
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bioRxiv - Biochemistry 2024Quote: ... and HRP-conjugated anti-mouse IgG (1:2500, NA931V, GE Healthcare, Fairfield, CT) were used as secondary antibodies.
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bioRxiv - Genomics 2024Quote: ... GAPDH immunoreactivity was detected using an anti-mouse IgG (1:6000, GE Healthcare) diluted in 5% nonfat dry milk ...
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bioRxiv - Microbiology 2020Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with corresponding antibody and chemiluminescence was visualized according to the manufacturer’s instructions (ECL Advanced; GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... For monoclonal antibody production, the DNA encoding mouse KCTD19 (residue 654-793 aa, NP_808459.1) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-chromatin complexes were purified with Protein A conjugated to sepharose beads (GE Healthcare, 30 µL GE17-5280-01) for 2 hours at 4°C ...
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bioRxiv - Genetics 2020Quote: ... Three washes were performed with TBST followed by 1 h incubation with secondary antibody (ECL, HRP linked, GE Healthcare) and another series of washes ...
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bioRxiv - Microbiology 2019Quote: ... membranes were incubated for 1 hour with secondary α-rabbit antibodies conjugated with horseradish peroxidase (HRP) (GE healthcare, NA934) diluted by 1:10,000 in 5% skim milk in TBST ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were washed with TBS-T and incubated with HRP secondary antibodies (GE Healthcare Life Sciences, 1:2000), and immunobands were detected with a Pierce ECL Western Blotting Substrate (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified using MabSelect Protein A followed by Capto Q according to manufacturer protocols (GE Healthcare, Chicago, IL). Purified antibodies were concentrated and formulated to ≥25 mg/mL in PBS.
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bioRxiv - Neuroscience 2020Quote: ... before they were incubated with HRP-conjugated secondary antibodies (1:10000, NXA931 and NA934, GE Healthcare Europe, Freiburg, DE) also diluted in blocking solution for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactivity was detected using species-appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000; GE Healthcare, Cleveland, OH, USA). Western blots were imaged via HRP chemiluminescent detection (Azure Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibody conjugated with horseradish peroxidase was detected with ECL plus chemiluminescence kit (Amersham Biosciences/GE Healthcare, Piscataway NJ). The images were acquired with an HP Scanjet and densitometry determined using NIH ImageJ ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... An enhanced chemiluminescence (ECL)-horse radish peroxidase (HRP) conjugated secondary antibody (NA934V, 1:1000 dilution, GE Healthcare, Uppsala, Sweden) and SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the filters were incubated with HRP-conjugated secondary antibodies in the respective blocking agent (NA931V and NA934V, GE Healthcare) for 1-2 h at RT or overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were detected against mouse or rabbit IgGs and visualized with ECL Western blot detection solution (GE Healthcare) or Odyssey infrared imaging system (LI-COR ...
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bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...