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Citations for GE Life Sciences :
1151 - 1200 of 2138 citations for 6 Amino 2 4 difluorobenzotrifluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The harvested cells were lysed by pulsed sonication and the soluble fraction was affinity purified using Ni2+-Sepharose (Ni Sepharose 6 Fast Flow, GE Healthcare). The resulting MsLadC variants ...
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bioRxiv - Biophysics 2023Quote: ... The 6×His-tagged SUMO fusion proteins were purified using a HisTrap™ HP Ni2+ affinity column (GE Healthcare Life Sciences). The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare) in TBS
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bioRxiv - Neuroscience 2023Quote: ... Reaction products were electrophoretically resolved on 6% denaturing sequencing gel for 1 hour at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Nuclease activity quantification compared the densitometric intensity of cleaved versus un-cleaved DNA (ImageQuant ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was digested with the TEV protease overnight and further purified by gel filtration chromatography using a Superose 6 column (GE Healthcare), eluted in buffer C (25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... A 2-fold molar excess of LF was mixed with 925 pmoles of hexon and incubated on ice for 5 min followed by SEC on Superose 6 increase 10/300 (GE Healthcare) equilibrated with buffer D ...
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bioRxiv - Biochemistry 2024Quote: ... The N-terminal GFP-His10 tag was cleaved overnight with 3C protease before gel filtration chromatography by using a Sepharose 6 Increase 10/300 GL column (GE Healthcare). For the purification of apo proteins ...
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bioRxiv - Biochemistry 2024Quote: ... Bio-Beads were removed by centrifugation (250g, 10 min) and the supernatant was subjected to size-exclusion chromatography in a Superose 6 increase column (GE healthcare) equilibrated in SEC buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to ∼2 µM for cryo-EM sample preparation.
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bioRxiv - Biochemistry 2024Quote: ... The sample was concentrated to 100 µL using a 100 kDa MWCO concentrator and the concentrate was loaded onto a size-exclusion chromatography (SEC) column (Superose 6 Increase 10/30 GL, GE Healthcare), equilibrated in a buffer composed of 20 mM Tris-Cl (pH 8) ...
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bioRxiv - Immunology 2024Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was desalted by a Zeba spin desalting column (7 kDa MWCO) and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare). Fluorescein- labeling efficiency was analyzed by SDS-PAGE and fluorescence gel imaging (Amersham Typhoon FLA 9500 ...
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bioRxiv - Microbiology 2024Quote: ... The fractions containing L–P protein were further purified using a size-exclusion column (Superose 6 Increase 10/300 GL, GE healthcare) equilibrated with size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2024Quote: ... Fab was purified from clarified culture supernatant six days post-transfection using Ni-NTA affinity chromatography with IMAC Sepharose 6 Fast Flow resin (GE Healthcare). Gel filtration on a Superdex 75 10/300 column (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was desalted by a Zeba spin desalting column (7 kDa MWCO) and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare). Fluorescein-labeling efficiency was analyzed by SDS-PAGE and fluorescence gel imaging (Amersham Typhoon FLA 9500 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... with 10 mM PTI and PTI alone in water (2 mL) were loaded into 2 mL mini-dialysis tubes with 1 kDa molecular weight cut-off (GE Healthcare Bio-sciences Corp. USA). The tube was immersed in a 200 mL closed Duran flask which was placed in a water bath at 37°C for the duration of the experiment ...
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bioRxiv - Microbiology 2020Quote: 4 µg of amplified DNA samples were labelled with Cy3 or Cy5 (GE Healthcare). Experimental samples were labeled with Cy5 while reference pools were labeled with Cy3 ...
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bioRxiv - Molecular Biology 2020Quote: ... packed in-house with Sepharose Fast Flow 4 resin (GE Healthcare, Pittsburgh, PA, USA) (miRNA validation) ...
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bioRxiv - Microbiology 2019Quote: ... 4°C and applied to a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 ml/min ...
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bioRxiv - Biochemistry 2019Quote: ... 4°C and loaded on a HisTrap column (GE Healthcare, Chicago, Illinois, United States) for purification ...
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bioRxiv - Molecular Biology 2019Quote: ... chromatin lysates were incubated for 4 hours with protein-G sepharose beads (GE Healthcare). Beads were washed 3× with low-salt buffer (150 mM NaCl ...
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bioRxiv - Microbiology 2019Quote: ... 4°C and applied to a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 ml/min ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare), washed in lysis buffer and kept on beads in lysis buffer with 1 mM DTT at 4 °C ...
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bioRxiv - Biochemistry 2019Quote: Size-exclusion chromatography was performed at 4 °C on an AKTA FPLC (GE Healthcare). Approximately 10 mg of apoferritin (Sigma A3660-1VL ...
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bioRxiv - Biochemistry 2020Quote: ... 30μl of beads coupled with ProteinA or ProteinG (GE Healthcare 4 Fast Flow Sepharose), previously blocked with 0.5mg/ml chicken egg albumin (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was mixed with 4 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer supplemented to 50 mM imidazole pH 8.0 and suspension was applied on Flex-Column (420400-2510 ...
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bioRxiv - Neuroscience 2020Quote: ... Scans were performed using a 4-10 MHz endocavitary transducer (GE Healthcare, Milwaukee, WI). Patients were in lithotomy position with empty bladder ...
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bioRxiv - Cell Biology 2021Quote: ... 3D-SIM was performed using a DeltaVision OMX Version 4 Blaze microscope (GE Healthcare), equipped with 405- ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Protein A Sepharose™ 4 Fast Flow sepharose beads were purchased from GE Healthcare Life Sciences (Pittsburgh ...
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bioRxiv - Cancer Biology 2022Quote: ... for 4 h in T cell culture media composed of RPMI1640 (GE Healthcare, SH30027.01) supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2021Quote: ... 50 μl of pre-washed protein-G sepharose-4 fast flow (GE Life Sciences) was added to each tube and tumbled end-over-end for 4 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 ml of NHS-Activated Sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) was washed with 10-15 column-volumes of 1 mM HCl ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) coupled to a GFP nanobody expressed and purified by published protocols45 and incubated on a rocker for 1.5 hours ...
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bioRxiv - Microbiology 2019Quote: ... coli expressed SDAg6 to CNBr-Activated Sepharose 4 Fast Flow (GE Healthcare Life Sciences) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... MAbs were purified over Protein G Sepharose 4 Fast Flow resin (GE Healthcare; 17061802). The antibodies were buffer exchanged into PBS pH 7.4 prior to use in any cell based assays.
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was incubated with CNBr-Activated Sepharose 4 Fast Flow beads (GE Healthcare) coupled with an anti-GFP nanobody39 resin for 3 hours ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified from lysates with glutathione-Sepharose 4 Beads (GE Healthcare). His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... for mouse antibodies or Protein A Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for rabbit antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... were used in association with nPprotein A Sepharose 4 Fast Flow beads (GE Healthcare) whereas 10 μg of anti-Sir2 antibody (Santa Cruz ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and cleaved from GST resin overnight at 4°C with PreScission Protease (GE Healthcare). GST was affinity purified as described above and eluted using 15 mM reduced L-gluathione ...
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bioRxiv - Genetics 2024Quote: ... which were conducted in the presence of Protein G Sepharose 4 beads (GE Healthcare). For phospho-CTD ChIP ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting sera were purified with CNBr-activated sepharose 4 Fast Flow (GE Healthcare) conjugated with the same peptide ...
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bioRxiv - Neuroscience 2023Quote: ... with MOPS buffer and transferred overnight at 4°C onto nitrocellulose membranes (GE Healthcare) with Tris-Glycine transfer buffer (25 mM Tris-base ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated overnight at 4 °C with anti-GST antibody (GE Healthcare) at a 1/1000 dilution ...
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bioRxiv - Immunology 2023Quote: ... or polyclonal IgG affinity-purified with Protein G Sepharose 4 Fast Flow (GE Healthcare) from heat-inactivated and filtered plasma were mixed with 10 TCID50 of SIVmac239 at a 1:1 ratio (5 μl:5 μl ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to Protein G Sepharose 4 Fast Flow beads (GE Healthcare) preincubated with or without MLCK mouse monoclonal antibody (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysate was then loaded onto glutathione SepharoseTM 4 Fast Flow resin (GE Healthcare), and washed with Tris buffer ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) coupled to an anti-GFP nanobody expressed and purified by published protocols2 and incubated on a rocker for 2 hours ...