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Citations for GE Life Sciences :
1101 - 1150 of 2694 citations for Toll Like Receptor 5 TLR5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: IgGs were purified from plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences). Heat-inactivated plasma was diluted 10-fold with cold PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... the HisTrap FF column was additionally coupled to a HiTrap Heparin HP 5-ml column (GE Healthcare). Both proteins were subjected to TEV protease cleavage at 4 °C overnight and another HisTrap FF 5-ml column purification step ...
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bioRxiv - Molecular Biology 2021Quote: ... Extract was prepared as above and was passed through a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 10 CV of IMAC buffer ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... CirpA4 and CirpA5 were further purified by ion exchange chromatography (Mono Q 5/50 column, GE Healthcare). Homogeneous protein populations eluted in the peak (CirpA3 ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... protein extract was fractionated by anion exchange chromatography using a MonoQ 5/50 GL column (GE Healthcare), washed with 10 column volumes (CV ...
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bioRxiv - Immunology 2021Quote: ... affinity chromatography with in-line dialysis in PBS via HiTrap 5 ml desalting columns (GE Healthcare – 29048684) using an Akta™ Pure system (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... IFN-α2 protein was column-purified using Nap-5 columns (GE Healthcare, Chicago, IL; #17-0853-01), incubated with 2.5ul serum ...
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bioRxiv - Immunology 2020Quote: ... 5-10 μg denaturated proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Antibodies against HOIL1 (H-1 ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were clarified by centrifugation and purified through a 5 mL HisTrap FF column (GE Healthcare), using theÄKTA Pure chromatography system (Cytiva) ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to affinity purification with a His-Trap HP 5 mL (GE Healthcare) column ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted in 1 × TBST with 5% non-fat milk: anti-rabbit IgG (1:10,000; NA934V; GE Healthcare) and anti-mouse IgG (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-labeled SARS CoV2 5’UTR RNA was immobilized on streptavidin-coated sensor chips (GE Healthcare Lifescience) to a final concentration of 300 Resonance Units (RU)/flow cell ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM imidazole and 0.023% DDM) using an ÅKTA purifier FPLC system (GE Healthcare, Chicago, United States). The unbound proteins were eluted from the column with binding buffer until the absorbance at 280 nm reached a stable baseline value ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SE001 was purified from cell supernatants after 5 days of induction using Nickel Excel resin (GE Healthcare), followed by size exclusion chromatography with a Sephadex S200 column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted fractions were pooled and loaded onto a pre-equilibrated 5 ml HisTrap HP (GE Healthcare) column ...
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bioRxiv - Plant Biology 2023Quote: ... Fluorescence scanning of acrylamide gels was performed on an Amersham Typhoon 5 scanner (GE Healthcare Life Sciences) using excitation and emission wavelengths of ex488/em520 and ex635/em670 for Cy2 and Cy5 scanning ...
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bioRxiv - Bioengineering 2023Quote: ... A single-use 10 L Cell bag™ bioreactor (working volume: 0.5-5 L; GE Healthcare, USA) was placed on the rocker ...
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bioRxiv - Microbiology 2022Quote: ... the supernatant was loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 40 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by nickel affinity chromatography using a 5 ml HisTrap HP column (GE Healthcare). The eluted proteins were dialyzed into 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified using an anion exchange chromatography column (HiTrap Q HP, 5 mL; GE Life Sciences) followed by ammonium sulfate precipitation (20 to 30% ammonium sulfate saturation ...
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bioRxiv - Bioengineering 2023Quote: ... Three consecutives’ batches were performed in 5 L bioreactor using ReadyToProcess WAVE 25 system (GE Healthcare, USA). A single-use 10 L Cell bag™ bioreactor (working volume ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). MBP-CENP-E2605-2701 was purified using the same lysis buffer without imidazole and an MBP-Trap HP coloumn (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysates were incubated with 70⍰μl of streptavidin beads (5⍰ml; 17-5113-01, GE Healthcare) at 4° for 3⍰h ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... to a volume of 500 μL and exchanged with a NAP-5 column (GE Healthcare Life Sciences) to a buffer consisting of 20 mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified using nickel affinity chromatography with a 5 ml HisTrap HP column (GE Healthcare) followed by size exclusion chromatography on a HiLoad Superdex 75 16/600 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... cell lysate was circulated over a 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged CoCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was applied to a 5 ml HiTrap TALON crude column (GE Healthcare, Little Chalfont, UK), washed with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... was then purified by Ni-NTA using a His-TRAP FF 5-ml column (GE Healthcare®). Collected elutions were dialyzed into 10 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted protein was separated on a 5 ml HiTrap STREP column (GE Healthcare, Little Chalfont, UK), and eluted with 2 mM desthiobiotin ...
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bioRxiv - Bioengineering 2023Quote: ... The diluted sample was loaded onto the pre-equilibrated HiTrap Q FF 5 mL column (GE Healthcare) at a flow rate of 2.5 mL/min ...
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bioRxiv - Plant Biology 2024Quote: ... Fluorescence scanning of acrylamide gels was performed on an Amersham Typhoon 5 scanner (GE Healthcare Life Sciences) with Cy2 and Cy5 settings using the 488nm and 635nm lasers ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The following morning the sample was loaded onto 5 mL HiTrap QFF anion exchange column (GE healthcare) and then washed with 15 CV of buffer A (20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... filtered culture supernatant was purified with a 5 mL HisTrap FF Crude column (GE Healthcare Life Sciences) previously equilibrated in Buffer A (20 mM NaH2PO4 ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on the His trap HP 5 mL column packed with Nickel (GE Healthcare). FPLC was used for elution of the GST-His6x-TEV-KIX protein with 300 mM imidazole in 50 mM Tris-HCl buffer (150 mM NaCl ...
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bioRxiv - Immunology 2023Quote: ... the cortex was homogenized and resuspended with 5 ml 70% Percoll Plus (GE Healthcare, 17-5445-02) and 5 ml 37% Percoll Plus placed on top ...
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bioRxiv - Cell Biology 2020Quote: ... Protein input was detected using an anti-GST antibody (GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... anti-rat or anti-rabbit HRP-conjugated antibodies (GE Life Sciences) as appropriate ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies pre-adsorbed to protein G-sepharose beads (GE Healthcare) were added to the cell extract and the mixture incubated for 2 hr at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and Secondary horseradish peroxidase -linked antibodies: anti-Rat (GE healthcare; NA935), anti-Mouse (GE health-care ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-rabbit or antirat IgG-linked HRP secondary antibodies (GE Healthcare) followed by ECL (Advansta).
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bioRxiv - Cancer Biology 2019Quote: ... incubated with corresponding horseradish peroxidase-linked secondary antibodies (Amersham, GE Healthcare) for 1h at room temperature and detected by chemiluminescence imaging system (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... incubated with appropriate HRP-labelled secondary antibodies (GE Healthcare Life Sciences), washed as before and developed using ECL (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... The antibody was detected using the ECL plus reagents (GE Healthcare). Detection was done in a dark room ...
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bioRxiv - Systems Biology 2021Quote: ... primary antibodies and polyclonal goat anti-mouse HRP conjugated (GE Healthcare) secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... Secondary HRP-conjugated antibodies used were donkey anti-rabbit (GE Healthcare) and goat anti-mouse (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... for IgG antibody or LambdaFabSelect Agarose Beads (GE Healthcare Life Sciences) for Fab at 4°C on a rotating shaker overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies used was anti-rabbit HRP-conjugated from GE Healthcare.
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bioRxiv - Cancer Biology 2020Quote: ... and HRP-conjugated secondary antibodies: anti-rabbit (GE Healthcare Cat# NA934); anti-mouse (GE Healthcare Cat# NA931 ...