Labshake search
Citations for GE Life Sciences :
1101 - 1150 of 1237 citations for Recombinant Human FAS His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
-
bioRxiv - Microbiology 2022Quote: ... The protein was purified by nickel affinity chromatography and subsequently by gel filtration on a Hi load 26/60 Superdex-200 column (GE Healthcare, Milwaukee, WI, USA) equilibrated with 20mM HEPES (pH 7.5) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Then 6X-His-tagged wild type SufB precursor and mutant proteins were purified by Ni-NTA affinity column (Ni-NTA His trap, HP GE Healthcare Life Sciences-17524802) (23 ...
-
bioRxiv - Plant Biology 2022Quote: ... lysed and the protein purified by nickel affinity chromatography using the His- Trap HP column coupled with size-exclusion chromatography (SEC) using the HiPrep 26/60 Sephacryl S-200 column (GE Healthcare Life Sciences, UK). The protein was eluted into a buffer containing 30 mM Tris ...
-
bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and eluted with a linear gradient of imidazole (0–1,000 mM) in His-tag-binding buffer using the ÄKTA FPLC™ Fast Protein Liquid Chromatography system (GE Healthcare, Princeton, NJ, USA). The eluted protein peak was collected and dialyzed against buffer D containing 50 mM Tris–HCl (pH 8.0) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Eluate from the His purification was then further purified by size-exclusion chromatography using a Superdex 200 HiLoad 16/600 column (GE Healthcare: 28-9893-35) in CSF-XB buffer ...
-
bioRxiv - Plant Biology 2020Quote: ... 9 mL of filtered immune serum were purified against the soluble part of the recombinant protein (10 mg) covalently coupled to 1mL HiTrap NHS-activated HP resin (GE Healthcare) according to the manufacturer instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... Each recombinant segment was purified by Ni2+-affinity chromatography using HisTrap HP purification columns with a FPLC system (ÄKTA Purifier, GE Healthcare) with elution buffer consisting of 50 mM NaH2PO4/Na2HPO4 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...
-
bioRxiv - Microbiology 2020Quote: ... The supernatants were dialyzed into 20 mM sodium phosphate pH 7.4 with 500 mM sodium chloride and the recombinant RBD was purified by immobilized metal ion affinity chromatography (IMAC) using a HisTrap excel column (GE Healthcare). The purified protein was biotinylated using the E ...
-
bioRxiv - Microbiology 2020Quote: ... Fractions containing the recombinant proteins were pooled and injected into a size-exclusion chromatography column (Superose12 10/300 GL; GE Healthcare) equilibrated with 50 mM Bis-Tris pH 6.5 ...
-
bioRxiv - Microbiology 2021Quote: ... The recombinant protein was finally purified by size-exclusion chromatography (SEC) on a HiLoad 16/60 Superdex 75 column (GE Healthcare) in 10 mM Hepes pH 7.5 and 300 mM NaCl.
-
bioRxiv - Immunology 2022Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 RBD protein (SinoBiological) were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
-
bioRxiv - Microbiology 2021Quote: The native molecular mass of the recombinant proteins was determined by gel filtration using a Superdex 200 10/300 column (GE Healthcare), previously calibrated with protein standards (GE Healthcare) ...
-
bioRxiv - Immunology 2021Quote: ... The recombinant proteins were purified from supernatants by affinity chromatography with a Ni-agarose column (HisTrapTM HP, GE Healthcare, Chicago, IL), dialyzed against PBS ...
-
bioRxiv - Immunology 2022Quote: ... assay was performed to quantify the binding avidity of the recombinant mos-tri-RBD to the receptor hACE2 using BIAcore 8K (GE Healthcare) with NTA chips ...
-
bioRxiv - Microbiology 2020Quote: ... All recombinant pilins were produced with an N-terminal histidine tag for immobilized nickel affinity purification using 1ml HisTrap columns (GE Healthcare) per the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: The green state of PhoCl1 and PhoCl1 empty barrel recombinant proteins were further purified with size exclusion chromatography using SD200 column (GE Healthcare) and buffer exchanged to 20 mM Tris pH 7.4 ...
-
bioRxiv - Microbiology 2021Quote: ... Expression of the recombinant proteins was performed using the ExpiCHO Expression system (Thermoscientific) following purification using HiTrap Protein-A column (GE Healthcare). The purified protein was sterile-filtered and stored in PBS.
-
bioRxiv - Biochemistry 2020Quote: ... Cells were lysed using a French press and recombinant proteins were purified by nickel ion affinity chromatography (HisTrap HP, 1ml - GE Healthcare). The His6-SUMO tag was removed using Ulp1 protease (Li and Hochstrasser ...
-
bioRxiv - Biochemistry 2020Quote: ... Cell extract was clarified by ultracentrifugation at 140 000 x g at 4 °C for 45 min and recombinant CNOT7 protein was purified automatically using ÄKTAxpress FPLC system (GE Healthcare). The following protocol was used for automated purification ...
-
bioRxiv - Microbiology 2020Quote: ... the recovered recombinant proteins were further purified by size exclusion chromatography using a Sephacryl S-400 16/60 column (GE Healthcare). Fractions containing the protein were pulled ...
-
bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
-
bioRxiv - Microbiology 2024Quote: Size exclusion gel filtration chromatography was carried out using purified recombinant PE11 on an analytical SuperdexTM 75 column connected to AKTApurifier chromatography unit (GE HealthCare). Briefly ...
-
bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatants were filtrated and subjected to affinity chromatography using a 5 mL HisTrap HP His-tag purification column (GE healthcare, cat. # GE29-0510-21) operated by a peristaltic pump (Cytiva ...
-
bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
-
bioRxiv - Immunology 2021Quote: ... sterilized by filtration with a 0.4 μm filter and recombinant proteins were purified with HisTrap TALON FF crude or HisTrap Excel columns (GE Healthcare Life Sciences).
-
bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
-
bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrapTM (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
-
bioRxiv - Microbiology 2021Quote: ... Recombinant Mt RnhC was concentrated and further purified by size exclusion chromatography using a 16/600Superdex® 200 pg columns (GE Healthcare). Mt RnhC eluted in a single peak and the fractions were pooled ...
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
-
bioRxiv - Cancer Biology 2024Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
-
bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
-
bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
-
bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
-
bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
-
bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
-
bioRxiv - Immunology 2023Quote: ... The culture medium containing the secreted LALA-B3B9 recombinant immunoglobulin G (rIgG) was purified using the protein A affinity column (GE Healthcare, Chicago, IL). The eluted fractions from purification were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the purity of the purified antibody ...
-
bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were further purified by gel filtration on an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principal peaks were collected ...
-
bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
-
bioRxiv - Immunology 2021Quote: ... Expression of the recombinant proteins was performed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrap™ (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
-
bioRxiv - Cancer Biology 2019Quote: Human PBMC were isolated by centrifugation of whole blood on Ficol-Paque (GE Healthcare) gradient for 30 minutes (500g ...
-
bioRxiv - Physiology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque Plus (GE healthcare) density gradient centrifugation from donated blood of healthy volunteers ...
-
bioRxiv - Cell Biology 2022Quote: ... ON-TARGETplus Human TP53/PTGS1/PTGS2 siRNA SMARTpool by Dharmacon (GE Healthcare, Manchester, UK) was used ...
-
bioRxiv - Immunology 2022Quote: Human ACE2 was immobilized onto CM5 sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified S trimer or RBD of Omicron lineages were injected ...
-
bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
-
bioRxiv - Physiology 2019Quote: Human PBMCs were isolated by Ficoll-Paque (GE Healthcare Life Sciences, Pittsburgh, PA, USA) centrifugation from blood drawn from donors recruited from the Boston community as part of the Phenogenetic Project and ImmVar Consortium ...