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Citations for GE Life Sciences :
1101 - 1150 of 2611 citations for Pituitary Tumor Transforming 1 PTTG1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... Kinetic constants were determined by fitting 1:1 Langmuir interaction model to the binding curves in the Biacore T200 Evaluation Software (GE Healthcare). Analysis of all A/P variants were restricted to concentrations of 25nM and lower ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Reduced proteins were now purified from the lysate samples using SP3 magnetic beads (1:1 mixture of SpeedBead Magnetic Carboxylate Modified Particles 65152105050250 and 45152105050250, GE Healthcare). To enable protein binding to the beads ...
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bioRxiv - Bioengineering 2020Quote: A solution of 25 mg/L SC-SWCNTs and 2 mg/mL C16-PEG2k-ceramide in 1% sodium cholate was added to a 1 kDa molecular weight cutoff dialysis cartridge (GE Healthcare). The solution was dialyzed against water with multiple water exchanges for a week ...
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bioRxiv - Microbiology 2021Quote: ... All sensorgrams were corrected by subtraction of the corresponding blank channel in addition to the buffer background and the kinetic constant determined using a 1:1 Langmuir model with the BIAevaluation software (GE Healthcare). Goodness of fit of the curve was evaluated by the Chi2 value with a value below 3 considered acceptable.
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bioRxiv - Microbiology 2021Quote: ... Data were fitted to a 1:1 interaction model using the evaluation software provided by the manufacturer (GE Healthcare Life Sciences). Reliability of the results was assessed according to U-value < 15 and χ2 < 5%Rmax ...
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bioRxiv - Microbiology 2021Quote: ... The primary antigen-antibody complex was detected by incubating the blot with goat anti-rabbit IgG HRP (1:10000, Jackson-ImmunoLab) and sheep anti-human IgG HRP (1:5000, GE Healthcare) in 3% milk in PBS-T ...
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bioRxiv - Biochemistry 2019Quote: ... the peak size-exclusion chromatography F86E RAD51 elution fraction was serially diluted (5, 1:1 serial dilutions) and loaded onto a Superdex200 10/300 GL size exclusion column (GE Healthcare) equilibrated with MALS Buffer (150 mM KCl ...
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bioRxiv - Immunology 2021Quote: All sensograms were corrected by subtraction of the corresponding blank channel and the kinetic constant determined using a 1:1 Langmuir model with the BIA evaluation software (GE Healthcare). Goodness of fit of the curve was evaluated by the Chi2 value with a value below 3 considered acceptable
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bioRxiv - Plant Biology 2019Quote: The construct of full length AtMLKL2 (residues 1-711) with N-terminal GST tag was cloned into the pGEX-6P-1 vector (GE Healthcare), and was expressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Immunology 2019Quote: ... rVACV-eGFP-OVA-Early and rVACV-eGFP-OVA-Late were generated by transfecting plasmid DNA into BSC-1 cells infected with VACV-vRB21 at an MOI of 1 using the CellPhect Transfection Kit (GE Healthcare). The resulting rVACVs were plaque-purified 3× prior to characterization ...
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bioRxiv - Biochemistry 2021Quote: ... were flowed through with a concentration ranging from 0.05 to 100 nM and the data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... the corresponding cDNAs of mouse angulin-1 were amplified by PCR using KOD-Plus-Ver.2 DNA polymerase and subcloned into pGEX-6P-1 (GE Healthcare). To construct an expression vector for MBP-tagged N-ZO-1 (aa 1-862) ...
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bioRxiv - Biochemistry 2021Quote: ... A 1:1 combination mix of two of magnetic carboxylate beads was used (Sera-Mag Speed beads, #45152105050250, #65152105050250, GE Healthcare). Beads were prepared fresh by rinsing with water three times prior to use at a stock concentration of 20 μg/μL ...
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bioRxiv - Microbiology 2022Quote: ... Ten µg of a 1:1 mixture of hydrophilic and hydrophobic carboxyl-coated paramagnetic beads (SeraMag, #24152105050250 and #44152105050250, GE Healthcare) were added for each µg of protein ...
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bioRxiv - Cancer Biology 2022Quote: ... The binding affinity was determined by global fitting to a Langmuir 1:1 binding model within the Biacore Evaluation software (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
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bioRxiv - Microbiology 2024Quote: ... to reach 120 mM of NaCl and were further passed through a Hi-Trap Q HP 1 mL column to remove residual DNA before binding on a Hi-Trap heparin HP 1 mL column (GE Healthcare) and elution with a NaCl gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:3 and 3:1 (w/w) at a concentration of 1 mg/mL were separated on a Superdex 200 10/30 column (GE Healthcare) using a LC1100 HPLC System (Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µL of a 1:1 mix of two types of commercially available carboxylate beads (Sera-Mag Speed beads, 45152105050250, 65152105050250, GE Healthcare), and 60 µL ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was finally concentrated to 1–10 mg mL−1 using centrifugal concentrators with a cut-off at 100 kDa (Vivaspin 100, GE Healthcare), frozen in liquid nitrogen and stored at −80 °C in storage buffer containing 50 mM Mes-KOH (pH 7) ...
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bioRxiv - Biophysics 2024Quote: ... Pooled elution fractions were subjected to a 2nd FPLC stage using a 1 ml HiTrap Heparin-HP 1 ml column (GE Healthcare). The bound MBP-XerC was washed using heparin loading buffer [50 mM Tris pH 8 ...
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bioRxiv - Systems Biology 2024Quote: ... The Bone marrow donors was diluted to a ratio 1:1 with PBS and layered on Ficol density gradient (GE Healthcare), followed by a centrifugation step for 20 minutes at 850 g at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... heparin-treated blood was diluted 1:1 with sterile PBS and carefully layered onto Ficoll-Paque (GE Healthcare Bio-Sciences, Sweden). The tubes were centrifuged at 400 x g for 30 min at 20°C ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence detection of HRP-conjugated secondary antibody was carried out using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare). Where representative blots are shown ...
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibody was anti-mouse HRP (horseradish peroxidase)-linked (NA931, Amersham ECL Mouse IgG, HRP, GE Healthcare Life Science) diluted 1:10000 in 5% dry milk in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... and HRP-labelled anti-mouse secondary antibody before bands being detected using an enhanced chemiluminescence kit (GE Healthcare, Buckingham, UK). β-actin (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... the membranes were incubated with 10,000-fold diluted peroxidase-linked secondary antibody (NA931V for FLAG-BRCA2 and NA934V for beta-Actin; GE Healthcare) at room temperature for 4 hours ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Affinity purification of TaBGC1-specific antibodies from the antiserum was performed with a HiTrap NHS-Activated HP column (GE Healthcare), conjugated to TaBGC1 recombinant protein.
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bioRxiv - Microbiology 2020Quote: ... Biovar-specific LPS antibodies were purified by standard affinity chromatography as described by the manufacturer (GE Life Sciences, PA USA).
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bioRxiv - Microbiology 2020Quote: ... and the others were incubated with the indicated antibodies overnight at 4°C.Then mixed with the Protein G sepharose beads (GE Healthcare) for 2 h at 4°C.The immunoprecipitates were washed four to six times with the 293T lyses buffer (50 mM Tris-HCl ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... Chemiluminescence detection of HRP-conjugated secondary antibody was carried out using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and X-ray film ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used in this study are as follows: HRPO-linked anti-mouse or anti- rabbit IgG (both GE Healthcare); both the secondary antibodies were used at 1:3000 dilution in blocking solution (Aydogan et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... After washing the membranes four times for 5 min at room temperature in Washing buffer (PBS, 0.1% (w/v) Tween-20) appropriate HRP-coupled secondary antibodies (anti-mouse IgG (NA931, GE Healthcare), anti-rabbit IgG (NA934 ...
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bioRxiv - Systems Biology 2022Quote: ... Immunoblots were carried out with the antibodies listed above and visualized using the ECL Western Blotting Detection Reagents (GE Healthcare). In order to strip antibodies from the blots ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed and probed with 100 μl/well of a goat α-GST antibody (GE Healthcare Life Sciences, 27457701V) diluted 1:800 and incubated for one hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The second antibodies used for ELISA were as follows: HRP-linked anti-whole rabbit IgG donkey serum (GE Healthcare, #NA934) for detection of the positive control ...
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bioRxiv - Microbiology 2019Quote: ... secondary antibodies conjugated with horseradish peroxidase were used for detection by chemiluminescence using the Amersham ECL Prime Kit (GE Healthcare). Biochemical fractionation of infected cells was performed as previously described [19].
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bioRxiv - Cell Biology 2020Quote: Secondary antibodies used for western blotting were: Amersham ECL Donkey anti-Rabbit IgG HRP (GE Healthcare NA934V, RRID:AB_772211, lot 16921443) and Rabbit anti-guinea pig IgG (H+L ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was collected and the antibodies were purified on a HiTrap Protein-A column (GE healthcare, Little Chalfont, UK).
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bioRxiv - Biochemistry 2021Quote: ... Polyclonal anti-GST antibody (Carl Roth, 3998.1) was covalently immobilized to all four flow cells of a CM5 sensorchip (GE Healthcare) to a level of 8,000 response units (RU ...
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bioRxiv - Biophysics 2020Quote: The binding affinities of the Fab antibody fragment Fab705 for full-length MgtE were measured using MicroCal iTC200 (GE Healthcare). The full-length MgtE proteins were purified by a similar procedure to that described above but with SEC buffer [buffer B containing 0.05% (w/v ...
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bioRxiv - Immunology 2021Quote: ... extracellular domains were expressed in Drosophila S2 cells and purified by immunoaffinity chromatography with LB3.1 antibody followed by Superdex200 (GE Healthcare) size exclusion chromatography as described [65 ...
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bioRxiv - Immunology 2020Quote: ... The fractions containing the antibody were pooled and elution buffer was exchanged for PBS using NAP-25 columns (GE Healthcare). Purified VHH72hFc1X7 in PBS was quantified by absorbance at 280 nm using a Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with the appropriate horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (GE Healthcare), subsequently detected with ECL (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes incubated with HRP-conjugated secondary antibodies were incubated with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare #RPN2232) for 5 mins ...
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bioRxiv - Biochemistry 2024Quote: ... The washing step was followed by 30 min incubation with HRP-conjugated Mouse Anti-M13 monoclonal antibody (GE Healthcare, 27942101) diluted in PBST in 1:5000 ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... the membrane was treated with secondary ECL anti-rabbit or anti-mouse IgG HRP-linked antibodies (GE Healthcare, Pittsburgh, PA) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... or with horseradish peroxidase (HRP)-conjugated secondary antibodies followed by a 3-min incubation with a chemiluminescent substrate (GE Healthcare) for ChemiDoc imaging ...