Labshake search
Citations for GE Life Sciences :
1101 - 1150 of 1316 citations for 6 TERT BUTYL PYRIDINE 3 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... Mpro was eluted with 300 mM imidazole in the lysis buffer and then purified by size-exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in 50 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size-exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 20 mM HEPES pH 7.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size- exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 50 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2020Quote: ... according to manufacturer’s protocol and removed the FLAG tag and His6-tag with TEV digestion followed by reverse nickel affinity purification and sizeexclusion chromatography on a Superose 6 Increase column (GE Healthcare Life Sciences). We biotinylated the protein with BirA ligase as previously described32.
-
bioRxiv - Immunology 2022Quote: ... The spike proteins were purified from the supernatants on complete™ His-Tag Purification Resin before further purification with Superose 6 increase (S6i) 10/300 column (GE Healthcare Biosciences). The H1 HA gene derived from A/Michigan/045/15 H1 was cloned into pcDNA3.4 vector and contains a GCN4 trimerization domain (folded ...
-
bioRxiv - Microbiology 2020Quote: ... The sdAbs with a His-tag fused at C-terminus were purified over Ni Sepharose 6 Fast Flow (GE Healthcare, Boston, MA, USA) and eluted with 400 mM imidazole ...
-
bioRxiv - Biochemistry 2019Quote: ... were mixed together and incubated for at least 1 h at 4 °C before removing the excess of free TcB-TcC by SEC on a Superose 6 Increase 5-150 column (GE Healthcare Life Sciences). For TcB-TcC variants containing HVR-RBD chimeras ...
-
bioRxiv - Biochemistry 2020Quote: ... lysate was recovered and incubated for 2 h with pre-equilibrated Ni-NTA beads (Ni Sepharose 6 Fast Flow, GE Healthcare Life Sciences) in a binding buffer (50 mM Tris ...
-
bioRxiv - Immunology 2020Quote: Purified CoV-S trimers were concentrated to 1 mg/mL and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and passed through a Wyatt DAWN coupled to a Wyatt UT-rEX differential refractive index detector (Wyatt Technology) ...
-
bioRxiv - Immunology 2020Quote: Purified C105 Fab was incubated with SARS-CoV-2 S trimer at a 2:1 molar ratio per protomer on ice for 30 minutes prior to purification by SEC on a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences). Fab–S complexes were concentrated to 1.6 mg/ml in Tris-buffered saline (TBS) ...
-
bioRxiv - Biochemistry 2022Quote: ... SEC-HPLC was carried out using an Alliance e2695 HPLC system (Waters Co., Milford, MA, USA) equipped with a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and a 2998 photodiode array (PDA ...
-
bioRxiv - Biophysics 2023Quote: ... The digested protein was concentrated using a 100 kDa concentrator and further purified on a Superose 6 Increase 10/300 gel filtration column (GE Healthcare Life Sciences) equilibrated in an Mg2+-containing buffer (20 mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2023Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labeled protein on a Superose 6 gel-filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Free biotin (or dye) was removed using size-exclusion chromatography by loading the labelled protein on a Superose 6 gel filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Immunology 2023Quote: ... The EROS-NOX2-p22phox-7G5 Fab complex was concentrated and applied onto a Superose 6 Increase 10/300 GL column (GE HealthCare, Chicago, IL) in Buffer D (20 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2024Quote: ... samples were loaded onto a Superose 6 10/300 gel filtration column under the control of an ÄKTA Pure instrument (GE Healthcare Life Sciences). Proteins were eluted using 50 mM HEPES ...
-
bioRxiv - Biochemistry 2024Quote: ... The ultracentrifuged sample was purified by size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), equilibrated with buffer containing 20CmM Tris-HCl ...
-
bioRxiv - Genetics 2020Quote: ... Eluted fractions were diluted 3-fold with R buffer and loaded onto a HiTrap SP column (GE Healthcare). Rrp1 eluted at around 600 mM NaCl with a linear gradient of 0.1-1.0 M NaCl in R buffer ...
-
bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was transferred on a column containing 3 ml Ni-Sepharose High Performance resin (GE Healthcare), washed two times with lysis buffer containing 30 mM and 50 mM imidazole ...
-
bioRxiv - Biophysics 2020Quote: ... zGDNFmat -zGFRα1D1-3 was further purified using size exclusion chromatography using a Superdex 200 (16/600) (GE Healthcare) in 20 mM Tris (pH 7.0) ...
-
bioRxiv - Immunology 2020Quote: ... separated by SDS-PAGE using a 3-15% Tris-Acetate gel and transferred to nitrocellulose membranes (GE Healthcare). Densitometry analysis was performed by the Image J software (National Institutes of Health).
-
bioRxiv - Immunology 2020Quote: Buffy coats were diluted [1:3] with PBS and added to Leukosep tubes containing Ficoll-Paque (GE Healthcare). Tubes were centrifuged at 450g for 40 minutes and the resulting buffy layer extracted ...
-
bioRxiv - Neuroscience 2020Quote: ... age range: 20-29 years) were scanned in a 3-Tesla MRI scanner (DISCOVERY MR750, GE Healthcare, USA) using a 32-channel head-coil ...
-
bioRxiv - Immunology 2020Quote: Whole-blood leukocyte DNA was extracted using the Nucleon BACC 3 DNA extraction kit (GE healthcare, Buckinghamshire, UK). We genotyped patients using the Illumina OmniExpressExome-8 Bead Chip (Illumina ...
-
bioRxiv - Genomics 2022Quote: ... with an anti-rabbit IgG (goat polyclonal, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4301, RRID:AB_2650489) secondary antibody was used as a loading control ...
-
bioRxiv - Cancer Biology 2023Quote: ... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
-
bioRxiv - Immunology 2023Quote: ... The peak of properly refolded HLA-G2 was collected and buffer exchanged into HBS-EP (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA and 0.005% [v/v] Surfactant P20, GE Healthcare) for surface plasmon resonance (SPR ...
-
Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Membranes were washed with PBS-T for 3 × 10 min and were developed by ECL Prime (GE Healthcare) and imaged on a Biorad ChemiDoc MP Imaging System.
-
bioRxiv - Neuroscience 2023Quote: ... MRI images were acquired with the aid of a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI) and an 8-channel head coil ...
-
bioRxiv - Biochemistry 2019Quote: ... diluted to 0.5 mL in GF150 buffer and further purified via size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare and Life Sciences) with GF150 buffer at 0.5 mL/min ...
-
bioRxiv - Biochemistry 2019Quote: ... Metal chelate chromatography was performed at 4 °C using an IMAC zinc-Sepharose 6 Fast Flow column (2.6 × 20 cm, GE Healthcare Life Sciences, Amersham, UK) equilibrated with 1×PBS pH 6.0 ...
-
bioRxiv - Cell Biology 2020Quote: ... SNAP-tagged proteins were filtered on a Superdex 200 10/300 GL or Superose 6 Increase 10/300 GL column (GE Healthcare, Little Chalfont, UK) and then labeled with SNAP-surface-549 (New England Biolabs ...
-
bioRxiv - Biochemistry 2021Quote: Three microliters of S-D614G in 1.2 mg/ml was mixed with 1.2 equivalent of RBD-chAb45 and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... The eluted protein was further purified by size-exclusion chromatography (SEC) using a sizeexclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer (50 mM Tris-HCl (pH 7.6) ...
-
bioRxiv - Biochemistry 2021Quote: ... and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... The clarified extract was either directly loaded onto Superose 6 column following pre-clearing with 150 μl of Sepharose CL-2B (GE Healthcare, 17-0140-01) or subjected to Halo purification by incubating with 100 μl of HaloLink™ Resin (Promega ...
-
bioRxiv - Biophysics 2021Quote: ... The eluted S proteins were further purified by size-exclusion chromatography (SEC) using a Superose 6 increase 10/300 GL column (GE Healthcare, U. S. A.) in TBS buffer (50 mM Tris-HCl (pH 7.6) ...
-
bioRxiv - Cell Biology 2021Quote: ... diluted to 0.5 mL in GF150 buffer and further purified via size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare and Life Sciences) with GF150 buffer at 0.5 mL/min ...
-
bioRxiv - Biophysics 2022Quote: ... The caveolin-containing eluate was concentrated and further separated by size exclusion chromatography using a Superpose 6 Increase 10/300 GL column (GE Healthcare, Boston, MA, USA) in buffer containing 200 mM NaCl ...
-
bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation procedures were performed by mixing ~1.6 mg of total proteins and 30 μl of the IgG Sepharose 6 Fast Flow beads (GE Healthcare, Cat# 17-0969-01) at 4°C for 2h and precipitating the NTAP tagged Orc4 ...
-
bioRxiv - Biochemistry 2024Quote: ... The concentrated sample was then subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), in buffer containing 150 mM NaCl ...
-
bioRxiv - Biochemistry 2020Quote: ... Blots were then washed 3 times in TBS-T and bands visualized with Amersham ECL chemiluminescence reagent (GE Healthcare). Blots were stripped (62.5 mM Tris-HCl pH 6.5 ...
-
Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... sensorchip in HBS-EP buffer (10 mM Hepes pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% surfactant P20) (GE Healthcare). All immobilization steps were performed at a flow rate of 2 µl/min with a final concentration of 100 µg/ml.
-
bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...
-
bioRxiv - Genetics 2020Quote: ... then washed for 3 × 20 min in 1× SSC and 0.1% SDS and imaged using a phosphorimager (GE Healthcare) and scanned using an Amersham Typhoon 5 scanner ...
-
bioRxiv - Bioengineering 2020Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
-
bioRxiv - Biochemistry 2021Quote: ... flow rate 0.8 ml/min) or Superdex 200 Increase 5/150 (~3 ml, flow rate 0.45 ml/min) columns (GE Healthcare) were used ...
-
bioRxiv - Cell Biology 2021Quote: ... SidK1-278-3×FLAG was further purified with a Superdex 200 10/300 Increase gel filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.4 and 150 mM NaCl ...
-
bioRxiv - Biophysics 2021Quote: ... Samples were concentrated L using a 3 kDa Vivaspin™ 20 filter column (GE Healthcare, Mississauga, ON, μ Canada). The conformational mixtures were purified via size exclusion chromatography (SEC ...
-
bioRxiv - Biophysics 2021Quote: ... 3 mM CaCl2 and 0.02% DDM) was injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), equilibrated with buffer (25 mM HEPES ...