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Citations for GE Life Sciences :
1101 - 1150 of 1629 citations for 6 Methyl 4 trifluoromethyl nicotinonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... the eluted protein solution was concentrated 10 times in a Vivaspin concentrator 30 kDa cut off and loaded in a Superose 6 increase 10/300 (GE Life Sciences) with PBS as a carrier buffer ...
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bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: Secreted candidate proteins were purified by immobilized metal ion affinity (IMAC) using Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL, EUA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The eluent was concentrated and further purified by using a size-exclusion chromatography (SEC) column (Superose 6 increase 10/300 GL, GE Healthcare, USA) in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The samples (equivalent to ∼85 µg microsomes) were then analyzed with an HPLC system and injected to a Superose 6 column (GE Life Sciences), equilibrated in a running buffer containing 25 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: The protein was purified from filtered cell supernatants using Strep-Tactin resin (IBA) before being subjected to additional purification by gel filtration chromatography using a Superose 6 10/300 column (GE Healthcare, USA) in 1 × PBS ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by addition of 6 mM β-mercaptoethanol and mixtures were applied to a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Sample homogeneity was investigated by analytical size exclusion chromatography on a Superose® 6 10/300 GL gel filtration column (GE Healthcare). Aliquots of purified EFL at 0.4 mg/mL were flash frozen in liquid nitrogen and stored at −70 °C.
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bioRxiv - Microbiology 2022Quote: ... The leukocyte-depleted red blood cell pellet was then resuspended in 10 ml PBS and 5 ml were carefully layered on 6 ml of 70% (v/v) isotonic Percoll cushion (GE Healthcare, UK). After centrifugation at 1200 x g for 15 mins ...
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bioRxiv - Genetics 2023Quote: ... GST-Clr4 and MBP-Clr6 were expressed in Escherichia coli BL21 (DE3) cells and purified on Ni+ SepharoseTM 6 Fast Flow (for 6His fusions; GE Healthcare, 17531806), Glutathione SepharoseTM 4B (for GST fusions ...
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bioRxiv - Biophysics 2022Quote: ... The concentrated protein sample was subjected to further size-exclusion chromatography (SEC) by a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare, USA) using a flow rate of 0.3 mL/min and a running buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Physiology 2023Quote: Plasma samples (200 μL) were loaded onto AKTA pure FPLC column [Superose™ 6 Increase 10/300 GL FPLC column (GE Healthcare). Lipoproteins were eluted with PBS and 0.25 mL fractions were collected at a flow rate of 0.4 mL/min.
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
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bioRxiv - Biochemistry 2023Quote: Oligomerization of ClpL WT and mutants was investigated by size exclusion chromatography (SEC) using a Superose® 6 10/300 GL column (GE Healthcare) at 4°C in assay buffer supplemented with 2 mM ATP ...
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bioRxiv - Genetics 2024Quote: ... Barcoded beads were resuspended in lysis buffer (200 mM Tris-HCl pH8.0, 20 mM EDTA, 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% Sarkosyl (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: SEC-MALS experiments were performed using and Ä KTA purifier system connected to a Superose 6 Increase 10/300 GL column (GE healthcare) at a flow rate of 0.4 ml/min in running buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting ABC holotoxins were then separated from excess TcB-TcC by size exclusion chromatography on a Superose 6 increase 5/150 column (GE Life Sciences) equilibrated in labelling buffer ...
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bioRxiv - Cell Biology 2024Quote: Purified LZTR1 proteins were centrifuged at 12,000×g for 10 min before being applied to an analytical Superose 6 10/300 SEC column (GE Healthcare Life Sciences) using a buffer containing 50 mM Tris/HCl (pH 7.4) ...
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bioRxiv - Developmental Biology 2020Quote: ... The fusion proteins were affinity-purified on Glutathione Sepharose® 4 Fast Flow (GE Healthcare), and then bound to POROS GoPure XS (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was added to a slurry of Glutathione Sepharose 4 Fast Flow (GE Healthcare) and incubated for 2 h at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... the clarified supernatants were loaded in 4 mL of Ni2+-sepharose beads (GE Healthcare, Uppsala), washed with Binding Buffer (4 × 4 mL ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5 ml HiTrap column (GE Healthcare) loaded with cobalt(II)-chloride hexahydrate (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5 ml HiTrap column (GE Healthcare) loaded with cobalt(II)-chloride hexahydrate (Sigma#255599) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein-G Sepharose 4 Fast Flow and SAv-Sepharose beads were obtained from GE Healthcare Bio-Sciences AB ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was precleared with beads (Protein G sepharose™ 4 flast flow, GE Healthcare) for 1 hour at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were precleared with Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 30 min at 4 °C under rotation ...
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bioRxiv - Plant Biology 2021Quote: ... coli strain BL21 (DE3) and purified with Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) by following the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... Synaptosomes were purified from 4-5 cerebella on discontinuous Percoll gradients (GE Healthcare, Uppsala, Sweden). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... and a multi-frequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare, USA) was utilized to capture a VL image the transverse plane at approximately 50% the distance between the mid-inguinal crease and the proximal patella ...
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bioRxiv - Biochemistry 2022Quote: ITC measurements were performed at 4 °C using a MicroCal auto-iTC200 calorimeter (GE Healthcare). Samples were extensively dialyzed against a buffer containing 500 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... A second incubation overnight at 4°C with Protein G-magnetic Sepharose beads (GE Healthcare) was used to pull down protein:DNA complexes prior to washing in low salt (120mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... NHS-activated Sepharose 4 Fast Flow and Q-Sepharose Fast Flow was from GE Healthcare Life Sciences ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... Protein A Sepharose CL-4B and Glutathione Sepharose 4 Fast Flow were from GE Healthcare Life Sciences (Chicago ...
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bioRxiv - Biochemistry 2022Quote: ... Fy-4 was subsequently purified combining Ni-NTA affinity chromatography (HisTrap FF column, GE Healthcare) and SEC (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Biophysics 2022Quote: ... 200 mM NaCl and 4 mM β-mercaptoethanol using an Akta FPLC system (GE Healthcare). Pure His-tev-ecMSG or tag-cleaved ecMSG was concentrated to 40-80 mg/ml ...
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bioRxiv - Biophysics 2022Quote: SEC and DMC columns were prepared as described previously.4 Sepharose CL-4B (GE Healthcare) and Fractogel EMD SO3- (M ...
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bioRxiv - Molecular Biology 2023Quote: DNA was extracted from leaves of 4 week-old plants by Nucleon Phytopure (GE Healthcare) and total RNA was extracted from 3 week-old plants by RNeasy (QIAGEN ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...
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bioRxiv - Genomics 2023Quote: ... each AP2 domain was previously cloned into the pGEX-4-T1 vector (GE Life Sciences) and transformed into BL21- CodonPlus(DE3)-RIL E ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified from clarified supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Cell Biology 2023Quote: ... This was incubated at 4°C overnight with 2 units of PreScission protease (GE Healthcare) in cleavage buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads.
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bioRxiv - Biophysics 2020Quote: ... The flow-through was then collected and concentrated using a 100 kDa concentrator and further purified on a Superose 6 gel filtration column (GE Healthcare Life Sciences) in 20 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: Protein extracts (150 mg per sample) were incubated with 300 µl of IgG-Sepharose 6 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h at 4°C with gentle rotation (1000 rpm) ...
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bioRxiv - Plant Biology 2020Quote: ... Obtained samples were fractionated on the column Superose 6 10/300 GL (50 kDa - 5 MDa range, GE Healthcare Life Sciences, Äkta FPLC) at the rate 0.5 ml/min in 20 mM Tris-HCl pH7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was injected into a Ni2+-NTA agarose affinity column (containing 5 mL Ni2+-Sepharose 6 FF beads, GE Healthcare, Cat. 17531803). The column was incubated for 15 minutes and washed twice with 30 mL washing buffer (50 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... This was performed using an purifier system connected to a Superose 6 Increase 10/300 GL column (both from GE healthcare, Solingen, Germany) at a flow rate of 0.5 ml · min−1 in running buffer (50 mM Tris/HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and then incubated with 500-μl of Nickel-Sepharose 6 Fast Flow (GE Healthcare Life Sciences, Piscataway, NJ, USA) for 2 h at 4°C ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: Gel filtration was performed using fast protein liquid chromatography (AKTA-FPLC system) on a Superose 6 10/300 GL column (GE Healthcare Life Sciences) as described previously (Hořejší et al. ...