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Citations for GE Life Sciences :
1101 - 1150 of 3464 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... After dialysis the supernatant containing folded tetramers and dimers were subjected to Superose 6 prep grade XK 16/70 size exclusion column (GE Healthcare) purification using HFB buffer ...
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bioRxiv - Immunology 2020Quote: ... Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare). After purification ...
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bioRxiv - Biochemistry 2020Quote: Analytical gel filtration was performed on a Superdex 200 Increase 10/300 GL column or Superose 6 Increase 10/300 GL (GE Healthcare) pre-equilibrated with 20 mM HEPES pH 7.5 ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... Cross-linked proteins were further purified from aggregation products by size exclusion chromatography on a Superose 6 increase column (GE Healthcare) equilibrated with size exclusion buffer (10 mM HEPES pH 7.8 ...
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bioRxiv - Molecular Biology 2020Quote: ... The gels were visualized by the 6-FAM label on the RNA primer using the Typhoon 9500 FLA Imager (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... The recombinant S-6P was purified by strep-tactin resin (IBA) following by size-exclusion chromatography on Superose 6 10/300 column (GE Healthcare) in 10 mM Tris pH 8.0 and 200 mM NaCl (SEC buffer) ...
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bioRxiv - Molecular Biology 2021Quote: ... ERH-22-103-(Gly-Ser-Ser)7-PICS-1180-200 and ERH-22-103-(Gly-Ser-Ser)6-TOST-134-54 were further purified by HiLoad 16/600 Superdex 75 column (GE healthcare) in a buffer containing 20 mM MES ...
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bioRxiv - Microbiology 2020Quote: Molecular mass determination was carried out using gel-filtration chromatography with a Superose 6 Increase 10/300 GL (GE Healthcare, USA) column ...
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bioRxiv - Microbiology 2020Quote: ... Lysed cells were ultracentrifuged and the cleared supernatant loaded onto a Ni Sepharose 6 Fast Flow His Trap column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Cell Biology 2020Quote: Frozen aliquots of SARS-CoV-2 Spike trimer (Wuhan) and D614G mutant S trimer were thawed and purified by size exclusion chromatography (Superose 6 Increase 10/300, GE Healthcare) using 2 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The assembled capsids are subsequently purified from un-assembled dimer subunits using a Superose 6 10/300 GL size exclusion column (GE Healthcare). The small molecule allosteric modulators were purchased (Chem-Div ...
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bioRxiv - Immunology 2020Quote: ... Conjugated VLPs or NPs were then separated from free HA trimers by SEC on a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: P3 membranes were solubilized as described in the previous section and 200 μL of the detergent-solubilized supernatant was injected in a Superose 6 10/300 GL gel-filtration column (GE healthcare) equilibrated with 20 mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved eluate was concentrated using a centrifugal filter (50 kDa cut-off, Amicon) and then loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare). The peak fraction containing recombinant SKI complex was collected ...
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bioRxiv - Biochemistry 2021Quote: ... The complex was concentrated using an Amicon Ultra Centrifugal filter (MWCO, 100 kDa) and subjected to a Superose 6 Increase 10/300 GL column (GE Healthcare) that was pre-equilibrated with running buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was concentrated and purified by size exclusion chromatography (SEC) on a Superose 6 Increase 10/300 GL column (GE Healthcare) on ÄKTA™ Pure ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein containing elution fractions were concentrated on Amicon concentrator (100 kDa MWCO) and loaded a Superose 6 16/600 (GE Healthcare) pre-equilibrated in SEC buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The nanobodies were fused with a His-tag at C-terminus and purified over Ni Sepharose 6 Fast Flow (GE Healthcare) and eluted with 400 mM imidazole ...
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bioRxiv - Immunology 2021Quote: ... at room temperature for 18 hours and subsequently the mAb-spike complexes were purified using SEC (Superose 6 Increase; GE Healthcare) with TBS as a running buffer and concentrated with 10 kDa cutoff Amicon ultrafiltration units ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted supercomplex solution was concentrated as described above and further purified by size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare), preequilibrated with buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: Secreted WNTs were enriched from cell culture supernatants using the affinity chromatography resin Blue Sepharose 6 Fast Flow (17-0948-01, GE Healthcare) and analysed by Western blotting according to previously published protocols (Glaeser et al. ...
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bioRxiv - Biophysics 2021Quote: ... using a Shimadzu HPLC LC-20AD/RF-20A (488 nmex, 512 nmem) instrument and Superose 6 10/300 column (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: The GST-rNLRP1FIIND S969A-rDPP9 complex and rNLRP1FIIND proteins purified as described above were subjected to gel filtration (Superose 6, 10/30; GE Healthcare) in buffer containing 10 mM Tris pH 8.0 and 100 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... Protein was purified from filtered cell culture supernatants by StrepTactin resin (IBA) and by size exclusion chromatography using Superose 6 column (GE Healthcare) in 10 mM Tris pH8,150 mM NaCl or 2 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2021Quote: ... the protein was then mixed with equimolar KIFBP and subjected to size exclusion chromatography a second time on a Superose 6 column (GE Healthcare) equilibrated with gel filtration buffer ...
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bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
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bioRxiv - Microbiology 2022Quote: ... The complex was then purified and excess Fab92 removed by SEC using a Superose 6 Increase 10/300 column (GE Healthcare), equilibrated with 10 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Low-molecular-weight RNAs were separated on 6% acrylamide/7 M urea gels and transferred to a Hybond-XL membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were concentrated using centrifugal concentrators (Amicon, 100 kDa) and polished by size exclusion chromatography (SEC, Superose 6 Increase, GE Healthcare) in buffer containing 25 mM HEPES ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: Affinity-purified xtZnT8 was concentrated to 6-8 mg/ml and loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), and was further purified by size-exclusion chromatography (SEC) ...
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bioRxiv - Biochemistry 2022Quote: ... TEV proteases were then added to the eluted proteins to remove the GFP and strep tag before the elution was concentrated by a 50-kDa MWCO spin concentrator and injected onto a Superose 6 column (GE Healthcare) equilibrated in 100 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer plus 0.03% digitonin ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were concentrated and further purified by size exclusion chromatography (SEC) on a Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared by consecutive centrifugation at 5,000 and 18,000 rpm and incubated with IgG Sepharose 6 Fast Flow beads (GE Healthcare; Cat# 17096902) on a rotating wheel for 120 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Fractions containing the proteins were pooled and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer ...
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bioRxiv - Cell Biology 2022Quote: ... sjRop1 LPPs and the purified REEP1 proteins from the membrane fractions were further purified by size exclusion chromatography (SEC) on Superose 6 or Superdex 200 (GE Healthcare) columns ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were harvested and filtered for further purification by Histrap HP (GE) and Superose 6 increase 10/300 column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... All FLAG-tagged variants were concentrated using centrifugal concentrators (Amicon, 30 kDa molecular weight cut-off (MWCO)) and polished by size exclusion chromatography (SEC, Superose 6 Increase, GE healthcare) in SEC1 buffer (30 mM HEPES/KOH ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The urea concentration was set to 6 M and the sample was applied to a 5 mL HiTrap QFF anion exchange column (GE healthcare) and was eluted using a 30 CV long gradient from low salt buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to ∼2 µM for cryo-EM sample preparation.
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bioRxiv - Biophysics 2024Quote: SEC-MALS experiments were performed by loading a 160 μM nCPEB4-NTD with 0 mM NaCl sample into a Superose 6 increase 10/300 GL column (GE Healthcare) mounted on a Shimadzu Prominence Modular HPLC with an SPD-20 UV detector (Shimadzu ...
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bioRxiv - Neuroscience 2024Quote: Molecular masses of purified gephyrin and variants were detected by analytical size exclusion chromatography (SEC) using a Superose 6 increase 5/10 (GE Healthcare). Typically ...
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bioRxiv - Biochemistry 2024Quote: ... concentrated using a Vivaspin concentration with 100,000 MWCO and loaded onto a Superose 6 Increase 10/300 size exclusion chromatography column (GE Healthcare Lifescience) equilibrated with the same HEPES-dialysis buffer to remove thrombin ...
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bioRxiv - Biophysics 2024Quote: ... the remaining 10 ml of concentrated 6-His-AdhE in buffer A were loaded onto a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biophysics 2024Quote: ... were used with flow rates at 6 ml/min and loaded either by hand or by using an AKTA Purifier (GE Healthcare) following manufacturers guidelines ...
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bioRxiv - Biophysics 2024Quote: ... 300 mM imidazole and 0.6% C8E4 (tetraethylene glycol monooctyl ether) and concentrated before loading onto a Superdex 200 increase 10/300 GL column (GE Healthcare) equilibrated with buffer G containing no imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... A total reaction mixture of 500 µL was used for downstream size exclusion purification utilizing a Superose 6 10/300 column (GE Healthcare) to remove excess PKA and phosphorylation reagents ...
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20 mM HEPES ...