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Citations for GE Life Sciences :
1051 - 1100 of 4180 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Protein A-Sepharose CL-4B (20 µl, GE healthcare, NJ) was added and incubation continued for 2 h at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Ly6a-Fc fusion protein in HBS-P+ buffer (GE Healthcare) was immobilized to a protein A sensor chip at a capture level of approximately 1200-1500 response units (RUs) ...
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bioRxiv - Bioengineering 2023Quote: ... Ly6a-Fc fusion protein in HBS-P+ buffer (GE Healthcare) was immobilized to a protein A sensor chip at a capture level of approximately 1200 -1500 response units (RUs) ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred to 0.45-µm nitrocellulose membrane (GE Healthcare) for 2 h at 100V ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a nitrocellulose membrane (GE Healthcare #106000169) followed by blocking with 4% skim milk in PBS (phosphate-buffered saline)/0.1% Tween (PBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... We purified the protein using Ni–NTA resin (GE Healthcare). We mixed the eluted fractions from the His column with TEV (tobacco etch virus ...
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bioRxiv - Plant Biology 2024Quote: ... Separated proteins were transferred to the nitrocellulose membrane (GE healthcare). The membrane was incubated with 0.5% w/v BSA prepared in 1X TBST (Tris-buffered saline with 0.01% v/v Tween 20 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Proteins were immobilized on a CM5 chip (GE Healthcare #29104988) via amine coupling to achieve 500–1500 response units (RU) ...
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bioRxiv - Genomics 2024Quote: ... Proteins were subsequently transferred to a nitrocellulose membrane (GE Healthcare) by tank transfer at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... LMW and HMW protein kit were obtained from GE Healthcare Life Sciences (Little Chalfont ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were further purified using a Superdex 200 (GE Healthcare) size exclusion column ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to Amersham Protran Nitrocellulose Membrane (GE Healthcare), and membranes were blocked with Intercept Blocking Buffer-PBS (Li-COR ...
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bioRxiv - Cell Biology 2024Quote: ... PTEN protein was immobilized onto a CM5 chip (GE Healthcare), which was activated using a 1:1 mixture of 1-ethyl-3-(3-dimethyllaminopropyl ...
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bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... human peripheral blood mononuclear cells were recovered by gradient centrifugation on Ficoll-Paque Plus (GE Healthcare) and monocytes were further purified using CD14 microbead positive selection and MACS separation columns (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2024Quote: ... 40μg of each protein extract (total or nuclear protein extract) were fractionated by SDS–PAGE and transferred to PVDF membranes (GE Healthcare Life Sciences, Buckinghamshire, UK). These were blocked in 5% nonfat dry milk in TBS-T (20mM Tris-HCl pH=7.5 ...
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bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
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bioRxiv - Cell Biology 2020Quote: ... using siRNA SMARTpool ON-TARGETplus Human UBTD1 (80019) or ON-TARGETplus Non-Targeting Control siRNAs) (GE Healthcare). Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668 ...
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bioRxiv - Biochemistry 2022Quote: ... and human FANCD2 was further purified by gel filtration chromatography on a Superdex 200 column (GE Healthcare) equilibrated with Buffer B containing 200 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Human PBMCs were isolated from the blood by density gradient using Percoll (GE Healthcare, 17-5445-01). PBMCs were washed and resuspended in RPMI 1640 supplemented with 10% FBS.
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare, Cat#17-5442-03). B cells were purified by negative selection (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were obtained from buffy coat by density gradient centrifugation in Ficoll–Paque PLUS (GE Healthcare). PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Immunology 2024Quote: Analysis of antibody binding to mouse and human FcγRs was performed on a Biacore T200 (GE Healthcare) using anti-His or anti-mouse Fab capture ...
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bioRxiv - Immunology 2024Quote: ... Human monocytes were isolated from screened buffy coats by density centrifugation with Ficoll-Plaque Plus (GE Healthcare) followed by Miltenyi Biotec magnetic-assisted CD14+ cell sorting ...
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bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (GE healthcare). The membrane was blocked with 5% non-fat dry milk in PBS containing 0.1% v/v Tween 20 (PBST ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were electrotransferred onto a 0.2 μm nitrocelluose membrane (GE Healthcare) and blocked with 5 % skim milk in 0.1 % Triton in Tris Buffer Saline (T-TBS ...
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bioRxiv - Cell Biology 2020Quote: ... For western blotting proteins were transferred on nitrocellulose membrane (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... Secreted mAbs were purified using a Protein G column (GE Healthcare) and concentrated for use in downstream assays.
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bioRxiv - Microbiology 2021Quote: ... The labelled proteins were then visualized using ECL prime (GE Healthcare) and LAS3000 (GE Healthcare) ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred and immobilized onto a nitrocellulose membrane (GE Healthcare) by electrophoresis for 2 h at RT in a standard transfer buffer containing 20% methanol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The eluted proteins were desalted on a PD10 column (GE Healthcare). The lipoyl-tagged was cleaved by incubating with TEV protease overnight at RTP ...
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bioRxiv - Immunology 2022Quote: ... Secreted mAbs were purified using a Protein G column (GE Healthcare) and concentrated for use in downstream assays.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was collected and applied to calmodulin sepharose (GE Healthcare) pre-washed with binding buffer (10 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein was applied to a Resource Q column (GE Healthcare). The column was washed with a 130–200 mM NaCl gradient in Binding Buffer over 7 column volumes ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to a nitrocellulose or PVDF membrane (GE Healthcare). Membranes were blocked for a minimum of 1 hour at room temperature in 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred onto Amersham Hybond ECL-nitrocellulose membrane (GE Healthcare). Unspecific binding sites of the membranes were blocked with 5% non-fat dry milk-TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to 0.45 μM nitrocellulose membrane (GE Healthcare, 15269794) using a Mini Trans-Blot system (Biorad ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies pre-adsorbed to protein G-sepharose beads (GE Healthcare) were added to the cell extract and the mixture incubated for 2 hr at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were transferred onto a nitrocellulose membrane (Cat#GE10600002, GE Healthcare), which was blocked for 30 min and incubated with primary antibody in 5% nonfat dry milk in TBS-T (20 mM Tris base (Cat#26-128-3094-B ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was bound to HisTrap HP prepacked columns (GE Healthcare) pre-equilibrated in lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were injected on a Superdex 75 10/300 (GE Healthcare) to check the folded state of the protein.
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined with 2-D Quant kit (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Protein was purified using Superdex 200 10/300 column (GE Healthcare) in a buffer containing 50 mM HEPES pH=7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... on an ÄKTA pure protein purification system (Cytiva, formerly GE Healthcare) with a running buffer composed of 10 mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2020Quote: ... protein was loaded onto a 5 mL HisTrap column (GE Healthcare) and eluted with a linear imidazole gradient (20-350 mM ...
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bioRxiv - Microbiology 2021Quote: Protein G-conjugated agarose beads (GE Healthcare, Cat# 17-0618-02) equilibrated in IP dilution buffer were used to preclear the chromatin for 1 h at 4⁰C with rotation ...