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Citations for GE Life Sciences :
1051 - 1100 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Proteins were visualized by enhanced chemiluminescence (ECL, GE Healthcare) and digital images were acquired using a FujiFilm LAS-4000 Image Analysis system ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were further purified using Superdex 200 (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... Protein A sepharose bead was purchased from GE healthcare. On target plus smart pool HOP siRNA (# L-019802-00-0005 ...
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bioRxiv - Biochemistry 2024Quote: ... Labeled proteins were purified with a PD10 (GE Healthcare) gel filtration column pre-equilibrated with the storage buffers of proteins (hSSB1 ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then transferred on PVDF membranes (GE Healthcare) and detected after incubation with specific antibodies using ECL Clarity Western blotting Substrate (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... proteins were transferred to a PVDF membrane (GE Healthcare) using electroblotting buffer (50 mM tricine ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µL of protein-A Sepharose (GE Healthcare, #11514945) was added ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were transferred to a nitrocellulose membrane (GE Healthcare) and blocked 1 H with the appropriate blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred to a nitrocellulose membrane (GE Healthcare). 3% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto nitrocellulose membranes (RPN132D, GE Healthcare) for 1 h at 4°C using the Mini Trans-Blot Cell (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto nitrocellulose membranes (RPN132D, GE Healthcare) for 1 h at 4°C using the Mini Trans-Blot Cell (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... a set of ON-TARGET human siRNAs against Arp3 (J-012077-08, Dharmacon, GE Healthcare), Myh9 (J-007668-07 ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA of human gelsolin (UniProt ID: P06396) was purchased from Open Biosystems (GE Healthcare). The fragment encoding subdomains 4-6 (residues 434-782 ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: Human peripheral blood neutrophils were obtained from consenting healthy volunteers using discontinuous Percoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Biophysics 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque (GE Healthcare Life Science) from buffy coats donated by healthy ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... We isolated human peripheral blood mononuclear cells (PBMCs) using the Ficoll-paque gradient (GE Healthcare) and removed the adhered monocytes after induced plastic adherence with cell culture flasks ...
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bioRxiv - Plant Biology 2020Quote: ... the cells were lysed by sonication and the fusion protein was purified using HisTrap HP histidine-tagged protein purification column of AKTA pure FPLC system (GE Healthcare, Pittsburgh, PA). The purified protein was further dialyzed overnight and further purified with HiLoad 16/600 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... This cell supernatant was then loaded into protein A affinity column (HiTrap protein A high performance, GE29-0485-76; GE Healthcare Life Sciences,) using liquid chromatography system (GE AKTA Prime Plus) ...
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bioRxiv - Microbiology 2022Quote: ... Protein-protein interaction and protein complex sizes were estimated by comparing the stained protein bands with the native protein molecular weight markers range between 60 to 690 kDa (Amersham, GE Healthcare Life Sciences). Protein bands excised from the stained native protein gels were identified by MS analysis.
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated on SDS-PAGE and fluorescence was detected from protein gels using the Typhoon FLA 9000 scanner (GE Healthcare Life Sciences) using Cy3 settings (532nm excitation and 610PB filter).
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bioRxiv - Immunology 2024Quote: ... IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare, Life Sciences) dependent on culture volume ...
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bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...
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bioRxiv - Neuroscience 2022Quote: ... and 500 μg of total protein from the supernatants was precleared with Protein G Sepharose 4 Fast Flow (GE Healthcare, catalog #GE17-0618-01) for 2 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Importin-α and Ran proteins lacking GST were prepared by cleavage with PreScission protease (20 U/mg of fusion protein; GE Healthcare, Uppsala, Sweden) at 4 °C for 12 h ...
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bioRxiv - Biophysics 2023Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM βME) and eluted with 0-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Biophysics 2024Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM BME) and eluted with 10-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto polyvinylidene difluoride (PVDF) membrane (GE healthcare). The membrane was blocked with 5% dry milk in PBS containing 0.1% v/v Tween 20 (PBST ...
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bioRxiv - Biophysics 2021Quote: ... on an AKTA Pure 25L protein purification system (GE Healthcare). The column was washed with 10 column volume (CV ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was purified on an equilibrated HisTrap (GE Healthcare) and step-eluted in wash buffer (100 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... and proteins were transferred to nitrocellulose membranes (GE Healthcare, 10600007). Antibodies used for western blots are listed in Supplementary Table 1 ...
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bioRxiv - Immunology 2021Quote: ... and protein bands were visualized with ECL Prime (GE Healthcare) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... supernatants were purified using Protein G Sepharose beads (GE Healthcare), dialyzed against PBS ...
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bioRxiv - Genetics 2021Quote: ... proteins were detected with Amersham ECL Prime (GE Life Sciences). The following antibodies were used ...
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bioRxiv - Genetics 2021Quote: ... Proteins were transferred to Hybond-P PVDF membranes (GE Healthcare) in 1 × NuPAGE Transfer Buffer (Invitrogen by ThermoFisher ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via FPLC (AKTA prime system, GE Healthcare) using HisTrap HP columns and eluted in 25 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... The digested protein was subjected to Superdex 200 (GE Healthcare) size exclusion chromatography in PBS to yield to pure anchor unit.
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred on a PVDF membrane (GE Healthcare, 10600021) via wet electroblotting at 100 V in transfer buffer (20% methanol ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were electrophoretically transfected onto nitrocellulose membranes (GE Healthcare). Membranes were blocked in blocking buffer (Li-COR Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were detected with the AI-600 image (GE Healthcare) using SuperSignal West Dura Extended Duration Substrate (Thermo) ...
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bioRxiv - Immunology 2022Quote: ... a 1:1 solution of Protein A Sepharose (GE Healthcare) and Protein G Sepharose (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... Separated proteins were transferred onto nitrocellulose membranes (GE Healthcare Amersham) which were subsequently blocked in 5% milk PBS for 1 h at room temperature before being probed with relevant antibodies O/N at 4°C in 5% milk PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... on Protein G Sepharose 4 Fast Flow beads (GE healthcare). The samples were incubated at 4°C overnight and then washed with progressively more stringent conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... then with protein A-Sepharose CL-4B beads (GE Healthcare) for another 45 min ...
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bioRxiv - Cell Biology 2022Quote: ... and protein levels were quantified using ImageQuant software (GE Healthcare). Results were normalized to GAPDH and then to Sham values.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were concentrated using a Vivaspin concentrator (GE Healthcare) and passed through a 0.2 µm Ultrafree-MC centrifugal filter (EMD Millipore ...
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bioRxiv - Genomics 2020Quote: ... Resolved proteins were transferred to a nitrocellulose membrane (GE Healthcare) and immunoblotted with the following antibodies at a 1/1,000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... To immunoprecipitate sorLA proteins 70 μL GammaBind Beads (GE Healthcare) coated with sol-sorLA antibody was used for each sample ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was captured on glutathione sepharose beads (GE Healthcare) and eluted with 10 mM reduced glutathione ...
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bioRxiv - Microbiology 2021Quote: ... 240 mg of protein A-Sepharose CL-4B (GE Healthcare), and 1.9 ml of cell lysate ...
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bioRxiv - Microbiology 2021Quote: ... protein blots were imaged using the ImageQuant LAS4000 (GE Healthcare). The band intensity was quantified using Fiji software.