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Citations for GE Life Sciences :
1051 - 1100 of 5375 citations for Mouse Anti Hantavirus Nucleocapsid Protein Antibody 4958 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The ACE2-Ig and RBD-Ig fusion proteins secreted to the medium were purified on HiTrap Protein G High Performance column (Cat#GE17-0405-01, GE Healthcare). Control-Ig was one of the following fusion proteins ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at 16,000xg for 10 min and the clarified lysates were pre-adsorbed to Protein A or Protein G beads (GE Healthcare Bioscience) for 2 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Elution fractions containing the untagged protein were pooled and the homogenous protein was finally isolated by size-exclusion chromatography onto a Superdex 75 column (GE Healthcare) equilibrated in 20 mM Hepes/NaOH ...
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed using BCA and proteins was separated on SDS-PAGE followed by transfer to PVDF membrane (GE Healthcare). Next ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
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bioRxiv - Immunology 2024Quote: ... The antibody was purified from culture supernatant by protein G affinity chromatography using the ÄKTA Pure system with a HiTrap Protein G column (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... in a final volume of 260 µl and injected into the CodeLink Uniset Mouse I Bioarrays containing 10,000 mouse genes probes (GE Healthcare). Arrays were hybridized overnight at 37° C at 300 rpm (8 g) ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were detected using horseradish peroxidase-conjugated secondary antibodies (GE Healthcare). To detect the deGRadFP expression from the hb-deGRadFP construct ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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bioRxiv - Pathology 2021Quote: ... horseradish peroxidase linked antibodies were used as a secondary antibody (GE Healthcare). Each signal was detected using Immunostar Zeta or LD (Fuji film ...
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bioRxiv - Biochemistry 2021Quote: ... Secondary antibodies (GE Healthcare) were used at 1:5,000 for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-Scp160 antibodies (Gift of R. Jansen, U. Tuebingen) was performed using the Amersham ECL Western Blotting Detection Kit (GE Healthcare Life Sciences). Quantification of protein bands was performed using ImageJ software.
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bioRxiv - Developmental Biology 2021Quote: ... The bound protein was detected using rabbit anti-GH antibody at a final dilution of 1:40,000 (AFP5672099, NIDDK-NHPP) followed by horseradish peroxidase-conjugated antibody (anti-rabbit, IgG; GE Healthcare, Chicago, Illinois, USA) at a final dilution of 1:2000 ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed to remove unbound phages and then probed with anti-M13-HRP secondary antibody (1:5000, Cytiva [formerly GE Healthcare], Marlboro, MA) followed by TMB as noted above.
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bioRxiv - Molecular Biology 2023Quote: ... Analysis was done by western blotting using the primary antibodies directed against H3K36me3 (ab9050, 1:2000) or H3K36me2 (ab9049, 1:2500) and as secondary antibody the anti-rabbit HRP (Na934v, GE Healthcare, 1:5000). The signal was detected by chemiluminescence after the addition of Pierce™ ECL Western Blotting substrate.
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... H2AX (#2595S; Cell Signaling technology]. The membranes were extensively washed with 1% TBST followed by incubation with anti-isotype secondary antibody (GE Healthcare) conjugated with horseradish peroxidase in 5% skimmed milk at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... the primary antibody was visualized using 2~5 μg/ml secondary antibody towards specific species of primary antibody labeled with Cy3B (GE Healthcare) or Atto 488 (Sigma-Aldrich ...
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bioRxiv - Physiology 2019Quote: ... Equal loading of protein content was verified using alpha-tubulin as a housekeeping protein and bands visualised using ECL detection system (GE Healthcare, UK). Bands were measured using Image J densitometry ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted with an imidazole gradient and fractions containing the target protein collected using an AKTA Prime system (GE Life Sciences). Protein quality was analysed by Coomassie gel analysis ...
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bioRxiv - Microbiology 2019Quote: ... Protein was then purified using the AKTA protein purification system with a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02), followed by passage through a Superdex 75 column for size exclusion chromatography ...
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Biophysics 2022Quote: ... The fusion protein was purified by fast protein liquid chromatography (FPLC) using a 5ml HisTrap HP nickel column (GE Healthcare, Uppsala, Sweden) with 0.5M imidazole gradient (SKU I5513-100G ...
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bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: Non-specifically binding proteins were cleared from each sample by incubation with 60 µL of 20% Protein A-Sepharose™ CL-4B (GE Healthcare) in IP Buffer (150 mM NaCl / 1% Triton X-100 / 15 mM Tris-HCl ...
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bioRxiv - Neuroscience 2019Quote: ... full-length cDNAs (Human SOX5, clone ID 30343519; mouse Rarb, clone ID 30608242; mouse Rxrg, clone ID 5707723; all purchased from GE Healthcare) were inserted into pCAGIG vector (Addgene plasmid # 11159) ...
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bioRxiv - Cancer Biology 2021Quote: ... protein was transferred to nitrocellulose membrane (GE healthcare, RPN203D). Membranes were blocked for 1 hour at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were visualized using ECL detection reagents (GE Healthcare). Uncropped gel images for western blots are available in Supplementary information 3.
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bioRxiv - Cell Biology 2019Quote: ... proteins were transferred to nitrocellulose blotting membrane (GE Healthcare). The membrane was blocked with 3% w/v dried skimmed milk (Marvel ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred on 0.45μm nitrocellulose membrane (GE Healthcare), blocked with 5% milk with PBS-Igepal (PBS + 0,3% Igepal ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were bound on HisTrap (HP)(GE Healthcare) and eluted with increasing imidazole concentration ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein A-Sepharose 4 Fast Flow suspension (GE Healthcare) was washed with Tris-Buffered Saline (TBS ...
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bioRxiv - Immunology 2020Quote: ... using protein A affinity chromatography (GE Healthcare, Marlborough, MA). IgG from pooled samples that were collected from the macaques before immunization was purified as the control for passive antibody transfer ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein G beads from GE Healthcare (GE Healthcare, 10280243) were used to purify the target protein and then the protein sample was analyzed using western blot ...
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bioRxiv - Developmental Biology 2021Quote: ... Resolved proteins were transferred into nitrocellulose membranes (GE Healthcare). The following primary antibodies were incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred on PVDF membranes (GE Healthcare) and detected after incubation with specific antibodies using ECL Clarity Western blotting Substrate (Bio-Rad) ...
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bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein was bound to glutathione affinity resin (GE Healthcare), washed with 50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to hybond ECL membrane (GE Healthcare) and blotted with IL10Rα (AF-474-SP ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μL of Protein G-sepharose beads (GE Healthcare) coated with 12 µL rabbit anti-Bicc1 ...
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bioRxiv - Cancer Biology 2019Quote: ... using a ÄKTA Purifier protein purification system (GE Healthcare). His-tagged proteins were eluted with a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2020Quote: ... on an ÄKTA FPLC protein purification system (GE Healthcare) was used to further purify the Nbs and ensure their monomeric states ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were incubated with dithiothreitol (DTT -GE Healthcare) at 10 mM and 30°C for one hour ...
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bioRxiv - Biophysics 2021Quote: ... in an AKTA go protein purification system (GE Healthcare). The purified FRET constructs were divided into 200 μL aliquots ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins transferred onto nitrocellulose (GE Healthcare, Chicago, IL) or polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl samples of Protein A-Sepharose (GE Healthcare) were preincubated with 10 µl each of purified α-MyoE or α-Uso1 antibodies for 3 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Protein was transferred to a PVDF membrane (GE Healthcare) and blocked with 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... and Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads by dimethyl pimelimidate cross-linking as described in (69).