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Citations for GE Life Sciences :
1051 - 1100 of 2028 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The eluted protein was separated on a 5 ml HiTrap STREP column (GE Healthcare, Little Chalfont, UK), and eluted with 2 mM desthiobiotin ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was applied to a 5 ml HiTrap TALON crude column (GE Healthcare, Little Chalfont, UK), washed with lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted proteins were loaded onto a HiTrap Q HP 5 ml anion exchange column (GE Healthcare) pre-equilibrated with QA buffer ...
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bioRxiv - Neuroscience 2024Quote: ... We then loaded the cleaved samples onto a 5 mL HiTrap Q-sepharose FF column (GE Healthcare) and eluted the material with a gradient of 0%-100% buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was separated on a 5 ml HiTrap TALON crude column (GE Healthcare, Little Chalfont, UK), using washing in lysis buffer supplemented with 10 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... Rep ΔCys A97CA473C was purified by chromatography on a 5 ml His-trap FF column (GE Healthcare) using a 100 ml gradient of 5 mM to 1 M imidazole in buffer A ...
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bioRxiv - Microbiology 2024Quote: ... Clarified lysate was loaded onto a 5 mL His-Trap Ni-NTA column (GE Healthcare Life Sciences) using an Akta Pure (GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2024Quote: ... +4°C) and supernatant was loaded into a 5 mL Nickel sepharose column (HisTrap HP, GE Healthcare). The loaded column was first washed ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was loaded on 3 × 5 mL HiTrap™ DEAE Sepharose FF (GE Healthcare, Munich, Germany) equilibrated with the same buffer ...
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bioRxiv - Microbiology 2020Quote: ... F(ab’)2 fragment was purified though HiTrap Protein A High Performance column (GE Healthcare) and HisTrap High Performance column (GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... The gels were transferred for 2 hours onto nitrocellulose membranes (GE Healthcare Biosciences, Pittsburgh, PA). Detection was performed by diluting primary antibodies in TBS supplemented with 0.1% Tween and either 5% BSA or 5% non-fat dry milk ...
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bioRxiv - Microbiology 2020Quote: ... containing 1 mM 2-ME) and Superdex 200 Increase 10/300 GL column (GE Healthcare; 10 mM KPB [pH 7.0] containing 50 mM NaCl and 1 mM 2-ME) ...
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 RBD or NL63 RBD was immobilized to a CM5 sensorchip (GE Healthcare) using a Biacore T200 (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... 50 mM NaCl and 2 mM CaCl2) by PD-10 desalting column (GE Healthcare, USA). The cleavage reaction was performed with hEKL (370 nM ...
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bioRxiv - Biochemistry 2020Quote: ... loaded in a gravity column containing 2 mL of pre-equilibrated Ni-sepharose (GE Healthcare), and incubated under slow rotation for 30 min at 8 °C ...
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bioRxiv - Microbiology 2020Quote: ... Fixed cells were incubated with 2% ECL Prime Blocking Reagent (GE Healthcare, Piscataway, NJ, USA) in PBS at 25°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Filters were prehybridized for 2 hr at 65°C in Rapid-hyb solution (GE Healthcare): Hybridization was performed overnight in the same buffer containing a 32P-labeled probe ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and BSA was replaced by 2% charcoal dextran-treated FBS (HyClone, GE Healthcare Life Sciences).
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bioRxiv - Immunology 2022Quote: ... was synthesized by ABclonal Technology and got the 2 mg/mL purified antigen through Ni-IDA (GE Healthcare, USA). The mouse polyclonal antibodies against two parts of PGRP-S2 (NM_001163716.1) ...
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bioRxiv - Bioengineering 2021Quote: ... Immobilized metal affinity chromatography (IMAC) using His-Trap FF Ni+2-NTA column (GE Healthcare) attached to BioRad™ NGC system ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We purified all samples using a 2-D Clean-Up Kit (GE Healthcare, Uppsala, Sweden) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... lactose and 2’FL to RhLNBBP was measured using a Microcal ITC200 calorimeter (GE Healthcare). Titrations were performed in duplicates at 25°C with RhLNBBP (0.1 mM ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 cells were transfected with 40 nM small interfering (si)RNA (GE Life Sciences) and 2 μL (24-well plates ...
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bioRxiv - Plant Biology 2020Quote: ... The protein concentration was measured using 2-D Quant Kit (GE Healthcare, Piscataway, NJ, USA).
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bioRxiv - Plant Biology 2020Quote: ... and 2 mM EDTA at pH 7.4) and blotted onto Hybond-N+ membrane (GE Healthcare) using a Minifold I slot blot system (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred at 70 V for 2 h onto a nitrocellulose membrane (GE Healthcare) using a wet electroblotting system (BIO-RAD) ...
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bioRxiv - Plant Biology 2020Quote: ... GST-SRK2E and GST-SRK2E K50N proteins were expressed using pGEX6p-2 (GE healthcare, IL) and purified using Glutathione Sepharose 4B resin (GE healthcare ...
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bioRxiv - Immunology 2021Quote: ... RBD:2-15 complex was then isolated by gel filtration on Superdex-200 (GE Healthcare). Fractions containing complexes were pooled and concentrated to 6.5 mg/ml in SEC buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was incubated for 2 h with Glutathione 4B Sepharose (GE Healthcare, Germany) followed by washing of the Sepharose with 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2023Quote: ... Forty 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... 40 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was isolated and combined with 2 mL of Glutathione-Sepharose resin (GE Healthcare) and incubated for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... This was incubated at 4°C overnight with 2 units of PreScission protease (GE Healthcare) in cleavage buffer ...
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bioRxiv - Biochemistry 2022Quote: ... with 2×PBS buffer followed by further purification using a HiTrap Q column (GE Healthcare) with 25 mM HEPES-KOH buffer (pH 7.5).
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bioRxiv - Cell Biology 2022Quote: A full-length zebrafish calaxin sequence was subcloned into pGEX-6P-2 vector (GE Healthcare). For the mEGFP-Calaxin protein ...
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bioRxiv - Plant Biology 2022Quote: ... for PDX3 and ACTIN-2 and captured using an Amersham Imager 680 system (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... 2 mM TCEP via gel filtration (Superdex 75 Increase 10/300; GE Healthcare Life Sciences). Protein peak fractions were combined and concentrated to 1 mM ...
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bioRxiv - Plant Biology 2022Quote: ... The primary antibody was diluted 1:10,000 in TBST-Buffer with 2% Blocking (GE Healthcare) and incubated on the membrane for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... RNA (2 μg) of each group was spotted on a Hybond-N+ membrane (GE Healthcare), followed by UV cross-linking (120 mJ/cm2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred at 70 V for 2 h onto a nitrocellulose membrane (GE Healthcare) using a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... for 2 hours and then incubated with Streptavidin Sepharose High-Performance beads (GE Healthcare, 17511301) for 4 hours ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were then purified by cation exchange using a 5-mL HiTrap SP HP column (GE Healthcare) and the collected fractions analyzed by SDS-PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... The reducing agent was removed by passing twice through a NAP-5 desalting column (GE Healthcare, Chicago, IL) equilibrated with G-buffer lacking β-ME ...
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bioRxiv - Cell Biology 2020Quote: ... The cleaved dCas9 protein was further purified with a 5 ml SP Sepharose HiTrap column (GE Life Sciences) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants containing the protein extracts were then applied onto a HisTrap FF 5 ml column (GE Healthcare) loaded with nickel ions using an ÄKTA Start liquid chromatography machine (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant containing complexes of Panx and Sov was loaded onto 5 ml HisTrap Fastflow column (GE Healthcare). After extensive washing with Binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown at 37 °C with 5% CO2 in DMEM (HyClone, GE Healthcare life sciences) supplemented with 10% bovine serum and 1 U/mL penicillin/streptomycin ...
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bioRxiv - Biophysics 2020Quote: ... The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) using a column gradient from 0.1 to 0.4 M NaCl ...