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Citations for GE Life Sciences :
1001 - 1050 of 4247 citations for Recombinant Bovine CTLA4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified using a HisTrap HP column (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... 100 mM NaCl for all proteins with PD10 columns (GE Healthcare) and protein concentrations were determined by the Bradford assay (Biorad) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Separated protein samples were transferred to a nitrocellulose membrane (GE Healthcare). Following transfer ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were subsequently purified over a heparin column (GE Healthcare). Complexes were eluted by a linear gradient to 1 M NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred and immobilized onto a nitrocellulose membrane (GE Healthcare) by electrophoresis for 1h at 100V in standard transfer buffer containing 20% methanol ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were transferred onto 0.45 μm nitrocellulose membranes (GE healthcare). The membranes were blocked with 5% non-fat milk diluted in PBS 1X (Euromedex ...
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bioRxiv - Cancer Biology 2024Quote: ... Separated proteins were transferred to Hybond™ nitrocellulose membranes (GE Healthcare) using a semi-dry method (Bio-Rad Trans-Blot® Turbo™ Transfer System) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein purification was conducted using a Resource Q column (GE Healthcare), eluting TET2 using a linear gradient of Elution buffer over 10 column volumes ...
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bioRxiv - Synthetic Biology 2023Quote: ... An AKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare) containing a Ni-Sepharose affinity chromatography (HisTrap HP ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were buffered in PBS using NAP-5 columns (GE healthcare) or Zeba spin columns (Thermo Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... proteins were transferred to polyvinylidene difluoride (PVDF) membranes (GE Healthcare, USA), and then probed with primary antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected with ECL Advance kit (GE Healthcare,Poole UK).
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bioRxiv - Plant Biology 2024Quote: ... Eluted proteins were desalted using a Q10/ PD10 column (GE Healthcare) and quantified using a Bradford ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes were subsequently cultured in complete RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS; GE Healthcare Life Sciences, Logan, UT), 0.1 mg/ml penicillin-streptomycin ...
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bioRxiv - Neuroscience 2021Quote: ... Lipofectamine 2000 and plasmidic DNA were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Neuroscience 2020Quote: ... BV2 cells were seeded in 24 or 6 well plates 24 hours prior to the transfection assay in high glucose DMEM supplemented with 10% fetal bovine serum (FBS, GE Healthcare Hyclone) and 1% antibiotic-antimycotic (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Lipofectamine 2000 and siRNAs were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Genetics 2019Quote: ... 293T cell line was cultured in DMEM high-glucose medium with 5% HyClone® fetal bovine serum (GE Healthcare, Piscataway, NJ, USA) and were maintained at 37°C with 5% CO2.
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Molecular Biology 2019Quote: ... peroxidase-conjugated AffiniPure Bovine Anti-Goat IgG (Jackson Immuno Research 805-035-180) and peroxidase-conjugated Sheep Anti Mouse IgG (GE Healthcare NA931V). All peroxidase-conjugated secondary antibodies were used at a dilution of 1:5000 ...
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bioRxiv - Immunology 2022Quote: ... at a density of 0.3 x 106 cells/mL in 10 mL total volume/plate of complete culture medium (RPMI 1640 plus 10% heat-inactivated fetal bovine serum (FBS, GE Healthcare HyClone), 50 μM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...
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bioRxiv - Neuroscience 2021Quote: The SH-SY5Y cells were purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) and maintained in MEM/F-12 mixture containing 10% fetal bovine serum (FBS) (HyClone; GE Healthcare Life Sciences) and 1% penicillin-streptomycin ...
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bioRxiv - Neuroscience 2020Quote: The human neuroblastoma SH-SY5Y cells purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) were maintained in MEM/F-12 mixture containing 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences) and 1% penicillin-streptomycin ...
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bioRxiv - Physiology 2020Quote: ... and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fischer Scientific, Waltham, MA) supplemented with 10% Fetal Bovine Serum (FBS) (GE Healthcare HyClone Laboratories, Logan, UT), non-essential amino acids (GE Healthcare HyClone Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... Bovine rhodopsin can be further isolated by detergent solubilisation and affinity chromatography using a Concanavalin A resin (ConA, GE Healthcare Life Sciences, USA) as described in Edwards et al ...
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bioRxiv - Neuroscience 2023Quote: ... and digested using the Macs Neural tissue Dissociation kit (Miltenyl Biotec) and subsequent percoll gradients (30% percoll [GE healthcare], 10% fetal bovine serum) was used to purify myeloid cells and incubated to antibodies against Cd11b and CD45 ...
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bioRxiv - Microbiology 2021Quote: ... and SARS2-38 mAb proteins were produced in CHOEXPI or EXPI293F cells and affinity purified using HiTrap Protein A columns (GE Healthcare, HiTrap mAb select Xtra #28-4082-61). Purified mAbs were suspended into 20 mM histidine ...
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bioRxiv - Genetics 2020Quote: ... the protein extract was incubated with IgG Fast Flow Sepharose (GE Healthcare) and beads were washed with lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... was purified by Maltose Binding Protein Trap (MBPTrap)-affinity column (GE Healthcare) and eluted in 20mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was gel filtrated on Sephacryl S-200 HR (GE Healthcare) equilibrated with NH100 buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred onto PVDF membrane (0.2 μm, GE Healthcare Amersham) and immunoblotting was performed using the following primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Protein purification was performed with Glutathione Sepharose 4 Fast Flow (GE Healthcare) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... and proteins of interest were transferred to nitrocellulose membranes (GE Healthcare Amersham) for 40 minutes at 100 V in a Mini Trans-Blot apparatus (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... protein was further purified using a 5 ml Q column (GE Healthcare). Protein was exchanged into 10 mM Tris-HCl pH 8.0 buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to a 0.2 µm nitrocellulose blotting membrane (GE Healthcare) for one hour at 25 V in cold transfer buffer (NuPAGETM ...
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bioRxiv - Plant Biology 2022Quote: ... coli DH5a and the proteins purified using glutathione resin (GE Healthcare, USA) as described (Fliegmann et al. ...
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bioRxiv - Physiology 2022Quote: ... proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane (GE Healthcare, 15269894) for blotting at 80V for 60 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The eluted proteins were further purified by Superdex 75 (GE Healthcare, USA) and stored in 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: Ni-NTA purified S proteins were further loaded into Superdex200 (GE Healthcare), the fractions were harvested and analyzed by SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... and proteins blotted onto nitrocellulose membrane using a Novex system (GE Healthcare). Western blots were developed with Classico substrate (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein sample was loaded onto a Heparin FF column (GE Healthcare) equilibrated with 20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleaved protein was applied to a desalting column 26/10 (GE Healthcare) and thereby buffer exchanged to the storage buffer (40 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Molecular Biology 2019Quote: ... Concentrated protein was loaded onto a pre-equilibrated Superdex 75 (GE Healthcare) column containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: 30 µl of Protein G Sepharose Fast Flow bead slurry (GE Healthcare) was washed once with 1 ml ice-cold phosphate-buffered saline (PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Refolded protein was captured on SP Sepharose Fast Flow resin (GE Healthcare) and further purified by size-exclusion chromatography (GF200 ...
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bioRxiv - Biophysics 2022Quote: ... Unencapsulated protein was removed by desalting on Sepharose CL-4B (GE Healthcare). Liposomes were attached via streptavidin to a quartz slide passivated with biotinylated-BSA ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were further purified by ion-exchange column (Source-15Q, GE healthcare) and polished by gel-filtration column (Superdex-200 ...
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bioRxiv - Genomics 2020Quote: ... on a fast protein liquid chromatography purification system (ÄKTA Explorer; GE Healthcare), which was eluted with storage buffer (50mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... Bound proteins were cleaved with Thrombin Protease (GE Healthcare, 27-0846-01) overnight at room temperature in Thrombin Buffer (140 mM NaCl ...