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Citations for GE Life Sciences :
1001 - 1050 of 5304 citations for Human G protein coupled receptor 42 GPR42 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... after which they were purified using a G-25 illustra microspin column (GE Life Sciences). For direct measurement of DNA binding ...
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bioRxiv - Biochemistry 2019Quote: ... and excess free dyes were removed by Sephadex G-25 column (Nap-5, GE Healthcare). Labeling efficiency was estimated by measuring A280 ...
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bioRxiv - Developmental Biology 2019Quote: ... Synthesized riboprobes were purified with the use of a ProbeQuant G-50 column (GE Healthcare) and diluted with hybridization buffer (5× Denhardt’s solution ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.5 using a PD-10 desalting column with Sephadex G-25 resin (GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... PBMCs were isolated using Ficoll-Paque density gradient solution (density =1.077 g/ml; GE Healthcare). Peripheral blood was mixed in a 1:2 ratio with phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5) followed by the addition of 0.03 g Thiopropyl Sepharose 6B beads (GE Healthcare) (washed with binding buffer) ...
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bioRxiv - Biochemistry 2021Quote: ... PD-10 columns Sephadex G-25 (20×80 mm) were from GE Healthcare (cat# 17085101).
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bioRxiv - Microbiology 2021Quote: ... NiV-G was purified using a Superdex 200 10/300 Increase GL column (GE healthcare) equilibrated in PBS pH 7.4 and NiV-F was purified using a Superose 6 Increase 10/300 GL column (GE healthcare ...
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bioRxiv - Immunology 2020Quote: ... and layered onto lymphocyte separation medium (1.077 g/ml; GE Healthcare Europe GmbH, Freiburg, Germany). After centrifugation (800 × g ...
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bioRxiv - Microbiology 2020Quote: ... After reverse transcription they were then cleaned using Illustra MicroSpin G-50 columns (GE Healthcare), double stranded DNA (dsDNA ...
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bioRxiv - Biophysics 2023Quote: ... clarified 400,000 x g for 30 minutes and applied to a Resource Q (GE Healthcare) ion exchange column equilibrated with G buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Sephadex G-25 in PD-10 desalting columns were purchased from GE Healthcare (Buckinghamshire, UK). Fluorescein isothiocyanate (FITC)-conjugated plain ...
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bioRxiv - Biochemistry 2023Quote: ... and DTT was removed by gel filtration on PD Minitrap G-25 columns (GE Healthcare) after column equilibration with strictly degassed buffer.
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bioRxiv - Genetics 2024Quote: ... The RNA product was purified by illustra microspin G-50 column (GE Healthcare Life Sciences). 10 μg of the purified RNA was then polyadenylated by 4U poly(A ...
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bioRxiv - Microbiology 2024Quote: ... DC control RNA was then purified using G-50 columns (GE healthcare, 25-5330-01). All samples were then prepared for sequencing using the randomer library prep workflow protocol described in Smola et al ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... The GST-tagged proteins were expressed and purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) following previously published protocols (42) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc fusion protein was purified from the cell culture supernatants with the protein A Sepharose (GE Healthcare). The purified ACE2-mouse IgG2a Fc fusion protein was biotinylated with Sulfo-NHS-LC-Biotin (Thermo) ...
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bioRxiv - Cancer Biology 2022Quote: ... All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). Further details can be found in our previous publication [14].
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were clarified followed by protein A affinity chromatography with MabSelect SuRe LX protein A resin (GE Healthcare) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
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bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
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bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
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bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
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bioRxiv - Immunology 2019Quote: ... Purified IgGs were tested for binding to OVA in ELISA and in an SPR assay using a BIAcore T200 apparatus (GE Life Sciences, Piscataway, NJ) in a protein A capture format ...
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bioRxiv - Developmental Biology 2022Quote: Unbiotinylated ectodomain transfections were collected and quantified by ELISA using nickel-coated microplates and pooled for purification using nickel-sepharose columns (HisTrap HP, GE Healthcare, Chicago, IL, USA) and an AKTAxpress chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Slides were examined using an Olympus IX-71 inverted fluorescence microscope with a photometric CoolSNAP HQ charge-coupled device (CCD) camera driven by DeltaVision software (Applied Precision, Seattle, WA).
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated with horseradish peroxidase-coupled secondary antibodies and imaged using chemiluminescent substrate on an Amersham Imager 600 (GE Healthcare, Chicago, Illinois).
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bioRxiv - Immunology 2020Quote: ... Buffer-exchanged RBD was concentrated to 5 mL and covalently coupled to a 5 mL HiTrap NHS-activated Sepharose column (GE Healthcare Life Sciences) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: ... the membranes were incubated with secondary antibody coupled with Horseradish Peroxidase (anti-rabbit, anti-rat or anti-mouse, Amersham, GE Healthcare, 1/3000) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection and quantification were performed using HRP-coupled secondary antibodies and and an Image Quant LAS4000 chemiluminescence-based detection system (enhanced chemiluminescence) (GE Healthcare Life Science).
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bioRxiv - Microbiology 2020Quote: ... proteins were immobilized to a protein A sensorchip a level of ~500 response units (RUs) using Biacore 8000 (GE Healthcare) and a running buffer of composed of 20mM PB7.4 with 300 mM NaCl ...
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bioRxiv - Biophysics 2019Quote: The proteins and protein complexes were analyzed by analytical gel filtration using a Superdex 200 5/150 column (GE Healthcare) and the 1260 Infinity Bio-inert high-performance liquid chromatography system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was further purified by fast protein liquid chromatography (FPLC) using a Source-S cation-exchange column (GE Healthcare) (low salt buffer with 50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were further purified by gel filtration on a fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principle peaks were collected ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Biophysics 2021Quote: ... Protein-transferred PVDF membranes (GE Healthcare Life Science) were blocked with 5% skim milk in PBS-T ...
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bioRxiv - Biophysics 2021Quote: ... proteins were visualized using ECL prime (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: M-PER mammalian protein extraction reagent (GE Healthcare) was used to extract protein from mOSE cells and run on NuPAGE 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... Protein transfer onto nitrocellulose membranes (GE Life Sciences) was carried out for 90 min at 25 W in a Mini Trans-Blot Electrophoretic Transfer Cell (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were exposed with ECL prime (GE Healthcare) at a ChemiDox XRS imaging system (Bio-Rad).Primary antibodies were used against H3K27me3 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to nitrocellulose membrane (GE Healthcare) and detection was performed using anti-His primary antibody (Penta-His Antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Fast-flow Protein-A sepharose beads (GE Healthcare) (250 µl slurry ...
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bioRxiv - Microbiology 2021Quote: ... using a protein purification system (ÄKTApurifier, GE Healthcare). Elution of the proteins was achieved by using a linear gradient of imidazole from 4 mM to 500 mM with sodium phosphate buffer B (20 mM Na2HPO4 x 2H2O ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to nitrocellulose membranes (GE Healthcare), and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10 ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified on protein A column (GE healthcare). Proteins were buffer exchanged and concentrated in PBS pH7.4.
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bioRxiv - Biophysics 2020Quote: ... The protein A sensor chips (GE Healthcare Lifesciences) were loaded by injecting each ligand for 100 s until to reach a signal level between 100 and 120 arbitrary response units (R.U. ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 µl of Protein A agarose (GE Healthcare) was coupled with 2 µg of purified antibodies or 5 µl of antiserum ...
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bioRxiv - Biochemistry 2022Quote: Proteins were transferred onto nitrocellulose membrane (GE Healthcare) by wet blotting overnight at 8°C and 27 V ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were revealed with ECL Prime (GE Healthcare) and images were acquired using a LAS4000 Camera (GE Healthcare).