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Citations for GE Life Sciences :
1001 - 1050 of 2500 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... the cleared lysate was loaded at 4 °C on an HisTrap HP Ni2+–Sepharose column (GE Healthcare) following the manufacturer’s instructions and further purified by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2021Quote: ... Sperm were layered onto a discontinuous gradient consisting of 4 mL of 35% Percoll® (GE Healthcare) underlaid with 2 mL of 70% Percoll® ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was cleaved for 16 h at 4°C using 1U of thrombin (GE Healthcare) per 100 μg of total protein ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified on Fy-4 immobilized on a HiTrap NHS- activated HP column (GE Healthcare). Antibodies were eluted using Pierce Gentle Ag/Ab Elution Buffer (ThermoFisher).
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bioRxiv - Plant Biology 2022Quote: ... crude extracts (4 ml) were passed through 0.5 ml DEAE-Sephadex A-25 columns (GE Healthcare, USA). The bound GSL was digested on the column for 18 h at room temperature with ∼0.8U myrosinase/thioglucosidase ...
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bioRxiv - Molecular Biology 2024Quote: ... Femurs were placed in a 4-limb positioning jig and scanned in eXplore Locus scanner (GE Healthcare). The femur containing jig was immersed in water and specimens were scanned at an 18 µm voxel size using the following settings ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the supernatant was applied to a 1 ml Ni-NTA HisTrap column (GE Healthcare). The protein was eluted with an imidazole gradient using an analogous gradient of HisTrap buffer B (HisTrap buffer A with 500 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... of which 450 µl were subjected to isoelectric focusing on pH 4-7 IPG strips (GE Healthcare), followed by SDS-PAGE with 12 % acrylamide gels ...
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bioRxiv - Immunology 2023Quote: ... The secreted antibodies in the supernatant were purified by protein G Sepharose 4 Fast Flow (GE Healthcare). Purified antibodies were dialyzed in PBS and sterile filtered (0.22 μm) ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01). 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega) ...
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bioRxiv - Neuroscience 2023Quote: The binding of FUS to (G4C2)4 RNA was analyzed using a Biacore T200 instrument (GE Healthcare). (G4C2)4 RNAs biotinylated at the 5′ end in 10 mM HEPES pH 6.8 and 500 mM MCl (M = K ...
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bioRxiv - Cell Biology 2024Quote: ... coli BL21 Star (DE3) and affinity-purified on a Glutathione Sepharose 4 Fast Flow column (GE Healthcare), followed by ion-exchange chromatography on a POROS GoPure HQ column (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Immune complexes were incubated with Protein G Sepharose® 4 Fast Flow (GE Healthcare, #17-0618-01) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... using Micromanager 2.0.0. FRAP experiments (Fig. 4) were performed on an OMX-SR inverted microscope (GE Healthcare) with the following components ...
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bioRxiv - Microbiology 2021Quote: ... The resultant nickel column eluate was loaded onto a 5 ml SOURCE 15Q column (GE Healthcare), instead of 15S column ...
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bioRxiv - Biochemistry 2020Quote: Analytical size-exclusion chromatography (SEC) was performed on a Superose 6 5/150 column (GE Healthcare) in SEC buffer containing 10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was filtered (0.45 μm) applied to a 5 ml Ni-NTA column (GE Healthcare, Sweden) and eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was filtered (0.45 μm) applied to a 5 ml Ni-NTA column (GE Healthcare, Sweden) and eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was filtered (0.45 μm) applied to a 5 ml Ni-NTA column (GE Healthcare, Sweden) and eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Biophysics 2020Quote: ... and 5% glycerol at pH = 5.9 for HiTrap™ Heparin HP column (GE Healthcare Lifescience, 17040601). p53-R248Q is eluted with linear gradient of NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... The conditioned medium was flowed through a 5 ml His-Trap fast-flow (GE life sciences) column at a flow rate of 4 ml/min using an NGC chromatography system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: CMV clone 5 cells were maintained in McCoy’s 5A media without phenol-red (GE Healthcare SH30200.01), supplemented with 10% fetal bovine serum (Gemini Bio ...
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bioRxiv - Microbiology 2021Quote: ... Protein-containing fractions were pooled for desalting through a 5 mL HiTrap Desalting column (GE Healthcare). Desalted protein was dialyzed into a storage buffer (50 mM NaH2PO4 pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... 1mM DTT) and loaded onto a 5 ml Hitrap Q sepharose anion exchange column (GE Healthcare). A gradient from 0 % to 100 % high salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2022Quote: ... the first step purification was done on a 5 mL HisTrap™ HP column (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were labelled at the 5′ end with A647-maleimide or Cy5-maleimide conjugates (GE Healthcare) using the 5′ EndTag kit (Vector Labs ...
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bioRxiv - Bioengineering 2022Quote: ... with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow, 5 mL, GE Healthcare). Samples were eluted with imidazole ...
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bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare). Finally ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysate was applied to a 5 ml HiTrap HP Q column (GE Healthcare Biosciences). The flow-through ...
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bioRxiv - Biochemistry 2021Quote: ... and protein loaded onto a GSTrap FF 5 mL column (GE Life Sciences, Marlborough, MA, USA) using the NGC Chromatography system (Bio-RAD) ...
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bioRxiv - Biochemistry 2021Quote: ... The flow through was collected and loaded onto a MonoQ 5/50 GL column (GE healthcare) with buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare). The protein was dialyzed against 1X PBS overnight in the presence of 1:1000 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare) in 1XPBS pH 7.5 and eluted with 300 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified using anion exchange chromatography (Mono Q 5/50 GL, GE healthcare) pre-equilibrated with ion exchange buffer A (20 mM Tris ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto a Ni2+-NTA column (5 ml HisTrap FF column, GE Healthcare) and the protein was eluted with an imidazole gradient ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cleared by centrifugation and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–250 mM in 30 column volumes ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 times in TBST for 5 minutes and visualized using enhanced chemiluminescence (ECL, GE Healthcare). See Supplementary Table 2 for a list of antibodies used in this study ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the complex were then applied onto a 5 ml HiTrap Heparin column (GE Healthcare), and after washing with 5 CV of lysis buffer the bound material was eluted with 4 CV of Heparin elution buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Peak fractions containing bS1 were directly loaded on a 5 ml StrepTrap HP column (GE Healthcare) equilibrated with Strep binding buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... 32P phosphorylation signals were detected using an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare Life Sciences) and quantified using ImageQuant TL (Amersham Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... The resulting supernatant was added to 5 ml of Chelating Sepharose Fast Flow resin (GE Healthcare), which had been loaded with 0.1 M NiCl2 and equilibrated with 100 ml of 50 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... aSyn was buffer exchanged into 5% D2O and 95% H2O using PD10 desalting columns (GE Healthcare). CsI and NaCl were added to a final concentration of 150 mM and 1.5 M just before performing the experiments.
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bioRxiv - Plant Biology 2020Quote: ... The clarified cell lysate was first injected onto a 5 ml Ni2+-NTA column (GE Healthcare). The MBP-OsHIPP19-HMA/6xHis-SUMO-AVR-PikF complex was step-eluted with elution buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The cleared supernatant was loaded on a 5 ml HisTrap FF nickel affinity column (GE Healthcare), pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... diluted crude extract containing Nla28-DBD was loaded onto 5 ml MBPTrap HP columns (GE Healthcare) at a flow rate of 5 ml/min and washed with 600 ml cold column buffer at a flow rate of 10 ml/min on an ÄKTA Fast Protein Liquid Chromatography (FPLC ...
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bioRxiv - Molecular Biology 2020Quote: ... and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare). Finally ...
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bioRxiv - Microbiology 2020Quote: ... The lysate soluble fraction was loaded onto a 5 mL HiTrap chelating HP column (GE Healthcare) immobilized with 100 mM cobalt chloride and equilibrated with the lysis buffer ...