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Citations for GE Life Sciences :
1001 - 1050 of 2528 citations for 4 2 Methylimidazol 1 ylmethyl phenylamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... and incubated lysate equivalent to 2 mg of protein with 50 μL Streptavidin Sepharose High-Performance beads (GE Healthcare, IL, USA) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 3D widefield datasets were acquired using a 100x 1.4NA Uplan S Apochromat objective on a DeltaVision 2 Ultra microscope equipped with 7-Color SSI module and sCMOS camera and controlled by Acquire Ultra acquisition software (GE Healthcare), computationally deconvolved using the enhanced ratio constrained iterative deconvolution algorithm ...
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bioRxiv - Biochemistry 2020Quote: ... Nap1 containing fractions were concentrated to 2 mg/mL and loaded on a Superdex 200 10/300 GL gel-filtration column (GE Healthcare) pre-equilibrated with 25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... fluorescently labeled R18-HRSV or culture supernatants of HEp-2 were separated from excess R18 by a separation column (PD-10 Desalting Columns GE Healthcare). HRSV-R18 or culture supernatants of HEp-2 were incubated in suspension with A3.01 and HEp-2 cells at 4°C for 1 h to allow virus attachment ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplified products were separated by electrophoresis on a 2% agarose gel and quantified by using Image Quant TL analysis software (GE Healthcare). Quantitative real-time PCR was performed on a TP-850 Real-Time PCR Detection System (TAKARA Bio ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length nsp1 was synthesized as a gene fragment from Integrated DNA technologies (IDT) and cloned into the EcoR1 restriction site of pGEX-6P-2 (GE healthcare) using in-fusion cloning (TakaraBio) ...
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bioRxiv - Immunology 2020Quote: ... An equal volume of buffer (2 mM EDTA in PBS) and blood was mixed and carefully layered over 5ml Ficoll Hypaque solution (GE Healthcare). After centrifugation for 20 min at 900 g (with no break ...
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bioRxiv - Immunology 2021Quote: The binding affinities of antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Bioengineering 2021Quote: ... Sonications (10-ms bursts applied at 2 Hz for 100 s) were applied coincident with an injection of the MB ultrasound contrast agent Optison (GE Healthcare, Little Chalfont ...
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bioRxiv - Cell Biology 2020Quote: ... and differentiation was induced in differentiation medium (DM) for hMB consisting of DMEM (Nacalai) with 2% horse serum (HS) (HyClone; GE Healthcare) and PS.
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bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibodies were incubated for 2 h at room temperature and visualized on X-ray films using enhanced chemiluminescence detection kit (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... The remaining lysate supernatant was incubated for 2 h on a spinning wheel with 200 µl of high-Performance strepTactin Sepharose beads (#28-9355-99, GE healthcare). The beads were collected by centrifugation at 1600 g at 4°C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples (100 μl at 2 mg/ml) were resolved using a Superdex 200 10/300 analytical gel filtration column (GE Healthcare) running at 0.5 ml/min at 25 °C in 10 mM K-HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... Concentrated FhES (10 mg total protein) was fractionated (2 mL/fraction) by SEC on a Sephacryl S-200 HR 26/60 column (GE Healthcare) connected to an AKTA Start automated liquid chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2024Quote: IgGs were purified from ∼2 mL of plasma with an equal volume of Protein A Sepharose resin (GE Healthcare Life Sciences) and incubated with the resin for three days at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Samples were run for 2 h at 90 V and then scanned using a Typhoon FLA 9500 laser scanner (GE Healthcare) at a pixel size of 50 µm ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with blocking solution (2.5 % FBS, 200 mM glycine, 0.1 % Triton X-100 in PBS or 2 % bovine serum albumin (GE Healthcare or Calbiochem), 0.01 % Tween 20 (National Diagnostics or Roth ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Biochemistry 2023Quote: ... and 2 mM DTT) for 16 h before further purification by anion exchange chromatography on a HiTrap Q HP column (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2, cells were changed with fresh media containing RMPI1640 (Thermo, 21870) supplemented with 0.2% Hyclone FBS (GE Healthcare, SH30070.03) 100 ng/mL Activin A (R&D Systems ...
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bioRxiv - Microbiology 2023Quote: ... F045 and TriHSB.2 constructs contain C-terminal 6xHis tags and were purified by passage over a HisTrapFF column (GE Healthcare), followed by size exclusion chromatography using a Superdex 200 increase 10/300 column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Finally membranes were again washed three times with TBST and submerged in SuperSignal West Pico chemiluminescent substrate for 2 min before visualizing through ImageQuant LAS 4000 chemiluminescent Image analyzer (GE Healthcare). We used a variety of inhibitors including Torin 1 (inh-tor1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated for 2-h at room temperature with rabbit HRP-linked IgG (GE Healthcare, Chicago, IL, USA), and a chemiluminescence detection reagent (SuperSignal™ West Pico PLUS Chemiluminescent Substrate ...
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bioRxiv - Neuroscience 2023Quote: ... The PAGE gel was exposed to a phosphorimage screen for ∼2 hours and analyzed using a Typhoon imaging system (GE Healthcare). Band intensities corresponding to the uncleaved ribozymes and the two products of self-scission were analyzed using ImageQuant (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Neuroscience 2022Quote: ... The blots were visualised with the BioRad ChemiDoc XRS+ (Cohort 2) or the Amersham 6000 Gel Imager (GE Healthcare, Cohort 3), and quantified with Image Lab Software (BioRad) ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Biophysics 2022Quote: ... TCA extracts of total yeast or 2 μg of mitochondrial proteins were loaded on a 12 % SDS-PAGE and transferred onto a nitrocellulose membrane (GE Healthcare). UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD) ...
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bioRxiv - Genetics 2023Quote: ... (2) 75 µl blood to prepare Dried Blood Spots (DBS, Whatman® 903 generic multipart filter paper, GE Healthcare, Westborough, USA) for metabolic profiling ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of 100% ethanol or 10% DEET in ethanol was applied to a 2 cm x 6 cm strip of Whatman filter paper (GE Healthcare). The treated paper was hung from a rectangular plastic frame in front of the Peltier element ...
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bioRxiv - Immunology 2023Quote: The binding kinetics and affinity of monoclonal antibodies to the SARS-CoV-2 spike trimer were analyzed by SPR (Biacore T100, GE Healthcare). Specifically ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Microbiology 2024Quote: ... 50 µg of purified GST-HA-SspH1 was mixed with 2 µg in-house purified GST-tagged PreScission protease and pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare) in PreScission protease buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: The binding kinetics and affinity of identified compounds to PLpro of SARS-CoV-2 were analyzed by SPR using a Biacore T200 system (GE Healthcare) at 25 °C ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 mM DTT and loaded on a 5 ml HiTrap™ Phenyl Sepharose High-Performance column (GE Healthcare, Munich, Germany). The sample was eluted with a 1.0 to 0.4 M linear gradient of (NH4)2SO4 for 8 CV at a flow rate of 1 ml.min-1 ...
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bioRxiv - Microbiology 2020Quote: ... Response curves were fit to a 1:1 binding model using Biacore X100 Evaluation Software (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... and peroxidase-conjugated donkey anti-rabbit IgG antibody diluted in 1:2000–1:4000 (NA934, GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... The resulting data were fitted to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare). Also the ACE2 was immobilized onto CM5 chip and Serial dilutions of purified WT ...
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bioRxiv - Microbiology 2021Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated for 1□h at room temperature with anti-mouse (1:5000, GE Healthcare), anti-rabbit (1:5000 ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM TCEP) and applied it onto a 1 ml HiTrap SP HP Sepharose column (GE Healthcare). Cas9 was eluted using a linear gradient from 0-100% buffer B (20 mM Tris-Cl ...
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bioRxiv - Biophysics 2022Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... 500 μg of a 1:1 mix of hydrophobic and hydrophilic beads (GE Healthcare 65152105050250 and 45152105050250) was added to each sample ...
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bioRxiv - Microbiology 2020Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Kinetic constants were determined by the 1:1 Langmuir binding model using Biacore T200 software (GE Healthcare).