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Citations for GE Life Sciences :
951 - 1000 of 4096 citations for Tert Butyldimethyl 3 4 4 5 5 Tetramethyl 1 3 2 Dioxaborolan 2 Yl Phenoxy Silane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was loaded onto a 5 ml HisTrap column (GE Healthcare), which was preequilibrated with the Lysis buffer plus 20 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... which was used for affinity purification with 5 mL NiNTA column (GE Healthcare), which was previously equilibrated in NiA buffer (50 mM Tris pH8 ...
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bioRxiv - Neuroscience 2022Quote: ... After the reaction mixture had passed through a Nap-5 column (GE Healthcare) to remove excess dithiothreitol and iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: ... were loaded onto a 5-ml Ni-affinity column (HisTrap HP, GE healthcare) and washed with buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... and applied on a 5 mL HisTrap FF column (GE Healthcare Life Sciences) to remove residual His-tagged protein ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL of alkylation solution [100 mM iodoacetamide (GE Healthcare, Chicago, IL, USA)] was added and incubated protected from light for 15 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... prior to loading onto a 5 mL HiTrap Q IEX column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5 followed by a wash step with the same buffer until a conductivity of 5 mS/cm was reached ...
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bioRxiv - Molecular Biology 2023Quote: ... the eluate was applied to a MonoQ 5/50 GL column (GE Healthcare) equilibrated in 25 mM Tris-HCl pH 7.2 ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to a 5 ml His-TRAP column (GE Healthcare) and eluted with a gradient of 20 mM to 500 mM imidazole ...
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bioRxiv - Biophysics 2023Quote: ... supernatant was loaded onto a pre-packed 5 ml HisTrap column (GE Healthcare), equilibrated according to the supplier’s protocols with wash buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was passed through a 5 mL MBPTrap HP column (GE Healthcare) to remove the cleaved 6xHis-MBP tag ...
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bioRxiv - Biochemistry 2023Quote: ... and the filtrate loaded onto a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 10 CV of Nickel Wash Buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The filtrate was loaded onto a 5 mL HisTrap HP column (GE Healthcare), washed with 12 column volumes (CV ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then scanned using an Amersham Typhoon 5 imager (GE Healthcare) with a 685 nm laser (IRshort720BP20 filter ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were loaded into a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated with binding buffer (50 mM Tris ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified to homogeneity with a 5 mL HiTrap HP column (GE healthcare) attached to a ÄKTA pure purification system using a linear gradient of 0-250 mM imidazole in buffer A over 35 CV ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was loaded onto two 5 mL HisTrap HP columns (GE Healthcare). After the columns were washed with six column volumes (CVs ...
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bioRxiv - Biophysics 2024Quote: ... the supernatant was purified by IMAC (5 mL HiTrap Chelating HP, GE Healthcare) using an FPLC system (ÄKTAprime ...
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bioRxiv - Biochemistry 2024Quote: ... IAC was performed on HiTrap NHS-activated HP 5-mL columns (GE Healthcare) coupled with 2 to 3 mg/mL of either MNB4 (an anti-PTX3 monoclonal antibody recognising an epitope in the N-terminal domain that was used to purify PTX3 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and clarified lysates were applied to a 5 mL HisTrap column (GE Healthcare) that was equilibrated with Buffer A (20 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... the mixture was applied to a 5 mL HisTrap HP column (GE Healthcare) at a flow rate of 2.5 mL min-1 ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was loaded onto a 5 mL Ni-NTA column (GE Healthcare) connected to an ÄKTA Prime plus for purification.
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... The fusion proteins were affinity-purified on Glutathione Sepharose® 4 Fast Flow (GE Healthcare), and then bound to POROS GoPure XS (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was precleared with beads (Protein G sepharose™ 4 flast flow, GE Healthcare) for 1 hour at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were precleared with Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 30 min at 4 °C under rotation ...
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bioRxiv - Plant Biology 2021Quote: ... coli strain BL21 (DE3) and purified with Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) by following the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... and a multi-frequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare, USA) was utilized to capture a VL image the transverse plane at approximately 50% the distance between the mid-inguinal crease and the proximal patella ...
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bioRxiv - Biochemistry 2022Quote: ITC measurements were performed at 4 °C using a MicroCal auto-iTC200 calorimeter (GE Healthcare). Samples were extensively dialyzed against a buffer containing 500 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... A second incubation overnight at 4°C with Protein G-magnetic Sepharose beads (GE Healthcare) was used to pull down protein:DNA complexes prior to washing in low salt (120mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... NHS-activated Sepharose 4 Fast Flow and Q-Sepharose Fast Flow was from GE Healthcare Life Sciences ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... Protein A Sepharose CL-4B and Glutathione Sepharose 4 Fast Flow were from GE Healthcare Life Sciences (Chicago ...
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bioRxiv - Molecular Biology 2023Quote: DNA was extracted from leaves of 4 week-old plants by Nucleon Phytopure (GE Healthcare) and total RNA was extracted from 3 week-old plants by RNeasy (QIAGEN ...
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bioRxiv - Biochemistry 2022Quote: ... Fy-4 was subsequently purified combining Ni-NTA affinity chromatography (HisTrap FF column, GE Healthcare) and SEC (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Biophysics 2022Quote: ... 200 mM NaCl and 4 mM β-mercaptoethanol using an Akta FPLC system (GE Healthcare). Pure His-tev-ecMSG or tag-cleaved ecMSG was concentrated to 40-80 mg/ml ...
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bioRxiv - Biophysics 2022Quote: SEC and DMC columns were prepared as described previously.4 Sepharose CL-4B (GE Healthcare) and Fractogel EMD SO3- (M ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...
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bioRxiv - Genomics 2023Quote: ... each AP2 domain was previously cloned into the pGEX-4-T1 vector (GE Life Sciences) and transformed into BL21- CodonPlus(DE3)-RIL E ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified from clarified supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads followed by a HiLoad 16/60 Superdex 75 size exclusion chromatography.
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bioRxiv - Immunology 2024Quote: ... for 30 min at 4°C and with Streptavidin Sepharose High Performance beads (GE Healthcare) for 2h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) for 4h at 4°C with rotation ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for 1 hour prior to purification on a Superdex 200 10/300 size-exclusion column (GE Healthcare). Rac1 (G12V ...
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bioRxiv - Biophysics 2021Quote: ... After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare) on an FPLC (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: 50 μl of the N protein samples (1 mg/ml) purified according to protocols 1 or 2 were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Peak fractions were diluted with 2 volumes of buffer H and loaded onto a 1-mL SP HP column (GE Healthcare) and washed with buffer C (50 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of freshly purified protein (concentration 1-2 mg/ml) was injected on a Superdex 75 300/10 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Immunology 2022Quote: ... mononuclear cells were isolated by density gradient centrifugation of PBS-diluted buffy coat/blood (1:2) over Ficoll-Paque Plus (GE Healthcare). The PBMC layer was carefully removed and washed 3 times with PBS.