Labshake search
Citations for GE Life Sciences :
951 - 1000 of 1713 citations for Sheep Interleukin 6 IL6 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... The assembled capsids are subsequently purified from un-assembled dimer subunits using a Superose 6 10/300 GL size exclusion column (GE Healthcare). The small molecule allosteric modulators were purchased (Chem-Div ...
-
bioRxiv - Biophysics 2019Quote: ... 360562 RCF spin in a Ti70 rotor and incubated with 2 mL IgG beads (IgG Sepharose 6 Fast Flow, GE Healthcare) for 2 h ...
-
bioRxiv - Biophysics 2019Quote: ... 50 mM NaCl to a final concentration of ∼3 mg/ml further purified by size-exclusion chromatography using a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer ...
-
bioRxiv - Biophysics 2019Quote: ... The cells were lysed by sonication and the lysate was separated on a Ni sepharose 6 Fast Flow resin (GE Healthcare) equilibrated with 30 mM Tris-HCl pH 8 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The protein was concentrated to 500 µl and subjected to size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare) in gel filtration buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Immunology 2020Quote: ... Conjugated VLPs or NPs were then separated from free HA trimers by SEC on a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
-
bioRxiv - Immunology 2020Quote: ... mosaic NP preps were stored for a month at 4°C and then analyzed via SEC using a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: P3 membranes were solubilized as described in the previous section and 200 μL of the detergent-solubilized supernatant was injected in a Superose 6 10/300 GL gel-filtration column (GE healthcare) equilibrated with 20 mM Tris-HCl pH 7.8 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The cleaved eluate was concentrated using a centrifugal filter (50 kDa cut-off, Amicon) and then loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare). The peak fraction containing recombinant SKI complex was collected ...
-
bioRxiv - Molecular Biology 2019Quote: ... and boiled for 5 min at 95°C were separated on 6-8% polyacrylamide/7 M Urea denaturing gel in 1× TBE and transferred onto an Amersham HybondN+ membrane (GE Healthcare). RNA was UV-crosslinked to the membrane ...
-
bioRxiv - Biochemistry 2021Quote: ... The complex was concentrated using an Amicon Ultra Centrifugal filter (MWCO, 100 kDa) and subjected to a Superose 6 Increase 10/300 GL column (GE Healthcare) that was pre-equilibrated with running buffer containing 20 mM HEPES ...
-
bioRxiv - Biochemistry 2021Quote: ... the sample was concentrated and purified by size exclusion chromatography (SEC) on a Superose 6 Increase 10/300 GL column (GE Healthcare) on ÄKTA™ Pure ...
-
bioRxiv - Biochemistry 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... Mer2 constructs were eluted with a lysis buffer containing 1 mM maltose and passed through a 6 mL ResourceQ column (GE Healthcare) equilibrated in 50 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein containing elution fractions were concentrated on Amicon concentrator (100 kDa MWCO) and loaded a Superose 6 16/600 (GE Healthcare) pre-equilibrated in SEC buffer (50 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2021Quote: ... The nanobodies were fused with a His-tag at C-terminus and purified over Ni Sepharose 6 Fast Flow (GE Healthcare) and eluted with 400 mM imidazole ...
-
bioRxiv - Immunology 2021Quote: ... at room temperature for 18 hours and subsequently the mAb-spike complexes were purified using SEC (Superose 6 Increase; GE Healthcare) with TBS as a running buffer and concentrated with 10 kDa cutoff Amicon ultrafiltration units ...
-
bioRxiv - Biochemistry 2021Quote: ... The eluted supercomplex solution was concentrated as described above and further purified by size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare), preequilibrated with buffer (100 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2020Quote: Secreted WNTs were enriched from cell culture supernatants using the affinity chromatography resin Blue Sepharose 6 Fast Flow (17-0948-01, GE Healthcare) and analysed by Western blotting according to previously published protocols (Glaeser et al. ...
-
bioRxiv - Biophysics 2021Quote: ... using a Shimadzu HPLC LC-20AD/RF-20A (488 nmex, 512 nmem) instrument and Superose 6 10/300 column (GE Healthcare) in 20 mM Tris-HCl ...
-
bioRxiv - Immunology 2020Quote: The GST-rNLRP1FIIND S969A-rDPP9 complex and rNLRP1FIIND proteins purified as described above were subjected to gel filtration (Superose 6, 10/30; GE Healthcare) in buffer containing 10 mM Tris pH 8.0 and 100 mM NaCl ...
-
bioRxiv - Immunology 2021Quote: ... Media was harvested 4 days after transfection and the secreted protein purified using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography (SEC ...
-
bioRxiv - Immunology 2021Quote: ... Protein was purified from filtered cell culture supernatants by StrepTactin resin (IBA) and by size exclusion chromatography using Superose 6 column (GE Healthcare) in 10 mM Tris pH8,150 mM NaCl or 2 mM Tris pH 8 ...
-
bioRxiv - Cell Biology 2021Quote: ... the protein was then mixed with equimolar KIFBP and subjected to size exclusion chromatography a second time on a Superose 6 column (GE Healthcare) equilibrated with gel filtration buffer ...
-
bioRxiv - Biophysics 2019Quote: ... The protein solution was concentrated with a 100-kDa cut-off centricon (Milipore) and further purified by Superose-6 increase 10/300 column (GE Healthcare) using a buffer containing 50 mM Imidazole pH 6.0 ...
-
bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
-
bioRxiv - Microbiology 2022Quote: ... The complex was then purified and excess Fab92 removed by SEC using a Superose 6 Increase 10/300 column (GE Healthcare), equilibrated with 10 mM Tris pH 8 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Low-molecular-weight RNAs were separated on 6% acrylamide/7 M urea gels and transferred to a Hybond-XL membrane (GE Healthcare) by electrotransfer ...
-
bioRxiv - Biochemistry 2022Quote: ... The proteins were concentrated using centrifugal concentrators (Amicon, 100 kDa) and polished by size exclusion chromatography (SEC, Superose 6 Increase, GE Healthcare) in buffer containing 25 mM HEPES ...
-
bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
-
Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: Affinity-purified xtZnT8 was concentrated to 6-8 mg/ml and loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), and was further purified by size-exclusion chromatography (SEC) ...
-
bioRxiv - Biochemistry 2022Quote: ... TEV proteases were then added to the eluted proteins to remove the GFP and strep tag before the elution was concentrated by a 50-kDa MWCO spin concentrator and injected onto a Superose 6 column (GE Healthcare) equilibrated in 100 mM Tris-HCl ...
-
bioRxiv - Biophysics 2022Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer plus 0.03% digitonin ...
-
bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were concentrated and further purified by size exclusion chromatography (SEC) on a Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated with lysis buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared by consecutive centrifugation at 5,000 and 18,000 rpm and incubated with IgG Sepharose 6 Fast Flow beads (GE Healthcare; Cat# 17096902) on a rotating wheel for 120 min at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... Fractions containing the proteins were pooled and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... were acquired every 1–3 min over a total time period of 4–6 h and deconvolved using the softWoRx software (Applied Precision). In addition ...
-
bioRxiv - Cell Biology 2022Quote: ... sjRop1 LPPs and the purified REEP1 proteins from the membrane fractions were further purified by size exclusion chromatography (SEC) on Superose 6 or Superdex 200 (GE Healthcare) columns ...
-
bioRxiv - Genetics 2022Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare).
-
bioRxiv - Evolutionary Biology 2022Quote: ... 4 °C) and the supernatant was incubated with 75 μl of IgG Sepharose 6 Fastbeads (GE Healthcare, 52-2083-00 AH) by rotating at 4 °C for 2 to 3 hours to enable binding of the tagged protein ...
-
bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
-
bioRxiv - Microbiology 2022Quote: ... the supernatants were harvested and filtered for further purification by Histrap HP (GE) and Superose 6 increase 10/300 column (GE Healthcare) in 20 mM Tris pH 8.0 ...
-
bioRxiv - Molecular Biology 2022Quote: ... All FLAG-tagged variants were concentrated using centrifugal concentrators (Amicon, 30 kDa molecular weight cut-off (MWCO)) and polished by size exclusion chromatography (SEC, Superose 6 Increase, GE healthcare) in SEC1 buffer (30 mM HEPES/KOH ...
-
bioRxiv - Plant Biology 2022Quote: ... purified PIN1 and sybody-21 were mixed together with a molar ratio of ∼1:3 and then applied to size-exclusion chromatography using a Superose 6 Increase column (GE Healthcare) in buffer containing 25 mM HEPES pH 7.4 ...
-
bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
-
bioRxiv - Microbiology 2023Quote: ... 3 mg of proteins was used for immunoprecipitation with 50 ml of immunoglobulin G-Sepharose resin (IgG Sepharose 6 Fast Flow, GE Healthcare) or Anti-HA affinity matrix beads (Roche ...
-
bioRxiv - Immunology 2022Quote: ... Conjugated S-mi3 nanoparticles were separated from uncoupled S-6P trimers by SEC using a Superose 6 10/300 column (GE Healthcare) equilibrated with PBS ...
-
bioRxiv - Biochemistry 2022Quote: ... were solubilised in TK buffer supplemented with 6 M urea before loading onto a 5 ml HisTrap HP column (GE Healthcare). The column was first washed with TK + 6 M urea followed by TK + 6 M Urea + 50 mM imidazole ...
-
bioRxiv - Cell Biology 2023Quote: ... Analytical size-exclusion chromatography was carried out under isocratic conditions on a Superose 6 5/150 or Superdex 200 5/150 (GE Healthcare) equilibrated with SEC buffer (50 mM HEPES pH 8.0 ...