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Citations for GE Life Sciences :
51 - 100 of 2222 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... membranes were exposed for 10-30 s (in the case of GFP-Plk4) and for 3–5 min (in the case of actin) on a Amersham Imager 680 (GE Healthcare) and images were acquired with its CCD camera.
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bioRxiv - Genetics 2021Quote: ... The membrane was washed 3 × 5 min in PBST and then visualized by Amersham ECL Prime Western Blot Detection Reagent (GE Healthcare). Images were taken by Fluorescent Image Analyzer LAS-3000 (FujiFilm ...
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bioRxiv - Molecular Biology 2020Quote: ... and RNA primer 5’-rCrArGrUrGrCrUrArUrGrUr GrArGrArUrUrArArGrUrUrArU-3’ were prepared by solid phase synthesis on an ÄKTA oligopilot plus 10 (GE Healthcare). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55°C for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... by capillarity and membranes were hybridized with a 32P-labelled oligonucleotide probe (23S.1, 5’- GATTGCTCGAATGCCCAAAG-3’) using Rapid-hyb buffer (GE Healthcare). Radioactive membranes were exposed to Phosphorimaging screens and analysed using a Typhoon Tryo (GE Healthcare).
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bioRxiv - Cell Biology 2024Quote: All TIRF experiments (except Figure 3 F and G) were acquired at 37 °C/5% CO2 with the DeltaVision OMX SR microscope (GE Healthcare) with a 60×/1.42 NA oil Plan Apochromat objective (Olympus ...
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bioRxiv - Neuroscience 2024Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: Dried blood spot (DBS) samples were collected on Whatman 3-mm filter paper (Whatmann No. 3, GE Healthcare Life Sciences, PA, USA) from enrolled patients on Day 0 (before treatment ...
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bioRxiv - Molecular Biology 2020Quote: ... and subcloned into the pGEX-6P-3 (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... coli using the pGEX-5X-3 vector (GE Healthcare). The antiserum was affinity purified using the antigen coupled to Dyna M-280 Streptavidin beads (Life Technologies).
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bioRxiv - Biophysics 2024Quote: ... we labeled tubulin with Cy-3 (PA23001, GE Healthcare) for the optical trap experiments or ATTO647N (AD 647N ...
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated with GST or GST-tagged angulin-1 cytoplasmic region (409-575 aa or 409-570 aa) for 2-3 h at 4°C and further incubated with Glutathione Sepharose 4B beads (GE Healthcare) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Dynamic PET/CT imaging (2-3h) with arterial blood sampling was performed on Monkey 3 and Monkey 4 using a Discovery MI (GE Healthcare). Each animal had two baseline scans which were separated by one month for Monkey 3 and by one year for Monkey 4 ...
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bioRxiv - Biophysics 2022Quote: ... The TOP3A-RMI1/2 complex was assembled by incubating purified TOP3A and RMI1/2 (1:3 ratio) followed by purification using a Superdex 200 Increase (GE Healthcare) in buffer I.
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bioRxiv - Neuroscience 2022Quote: ... The blots were visualised with the BioRad ChemiDoc XRS+ (Cohort 2) or the Amersham 6000 Gel Imager (GE Healthcare, Cohort 3), and quantified with Image Lab Software (BioRad) ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Neuroscience 2020Quote: The blots were subsequently washed 3 x 5 min in TBST 1X and developed with the Prime Western Blotting detection Reagent (GE Healthcare Amersham) using the LAS3000 Bioimager (Fujifilm ...
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bioRxiv - Biochemistry 2020Quote: ... hydrophobic interaction chromatography using a Hi-Trap Butyl-Sepharose column (GE Healthcare, Cat. No. 28411001) with a flow rate of 1 ml/min and a gradient from 0.9 M (NH4)2SO4 in PBS to PBS over 5 CV was performed ...
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bioRxiv - Biophysics 2021Quote: ... coli cells were transformed with pGEX-4T-3 (GE Healthcare) containing the coding sequences of fascin ...
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bioRxiv - Cell Biology 2020Quote: ... we used a DeltaVision|OMX microscope version 3 (GE Healthcare) with an objective lens 100X UPlanSApo NA1.40 Oil (Olympus ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~7 mg/mL ...
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bioRxiv - Developmental Biology 2020Quote: GST fusion proteins were generated using pGEX5x.3 (GE Healthcare) as vector ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3) using pGEX4T-3 vector (GE healthcare, IL).
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bioRxiv - Immunology 2020Quote: ... and protein A-Sepharose (PAS, 3 mg/sample, GE Healthcare), after a preclearing step on PAS (1 h ...
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bioRxiv - Immunology 2024Quote: ... + 3 ml of isotonic Percoll (ISP; 90% Percoll (GE Healthcare) + 10% 1 M HBSS (Gibco)) ...
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bioRxiv - Biochemistry 2024Quote: ... each containing 3 mL of Heparin Sepharose resin (GE Healthcare), followed by incubation on a rocking shaker for 2.5 h at 6℃ ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing RNase E were pooled and loaded on a butyl sepharose HP column (GE Healthcare) which previously was equilibrated in high-salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Developmental Biology 2021Quote: ... 4-16-cell embryos were transferred to 3 % Ficoll (GE Healthcare) in 0.6x MMR and injected with 5-10 nl of a solution containing mRNAs and/or MOs ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% (v/v) ampholytes (pH 3-11 NL; GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
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bioRxiv - Immunology 2020Quote: ... cDNA constructs were inserted into the pGEX6P-3 plasmid (GE Healthcare) and expressed as GST-fusion proteins in High efficiency BL21(DE3 ...
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bioRxiv - Immunology 2021Quote: ... 3 or 4 of a Series S CM5 chip (GE Healthcare) functionalised with an anti-His capture kit (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Early passage HUVECs were coated on Cytodex-3 beads (GE Healthcare) at a density of 10 million cells/40 μl beads and incubated in suspension for 3-4 hours with gentle mixing every hour ...
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bioRxiv - Immunology 2020Quote: ... and 0.1 M 3-(N,N-dimethylamino) propyl-N-ethylcarbonadiimide (GE healthcare) at a flow rate of 5 µL/min ...
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bioRxiv - Microbiology 2021Quote: ... dadantii OutB was cloned into the pGEX-6P-3 vector (GE Healthcare) encoding a cleavable N-terminal Glutathione S-transferase (GST ...
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bioRxiv - Molecular Biology 2024Quote: ... using a multi-frequency linear array transducer (3-12 MHz, GE Healthcare). Participants were instructed to lay on an athletic training table in the supine position 5 minutes prior to imaging ...
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bioRxiv - Cell Biology 2024Quote: ... 3D-SIM was carried out using an OMX Version 3 (Applied Precision) microscope equipped with a UPlan APO 100× 1.4 NA oil-immersion objective (Olympus) ...
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bioRxiv - Biochemistry 2022Quote: ... and 3 mM MgCl2] using a size exclusion column (NAP5; GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... blotted for 3 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3 mM TCEP and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~10 mg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... or pH 3–10 for CDK2 (Amersham Biosciences, GE Healthcare Europe, Diegem, Belgium) before separation by SDS-PAGE (42 ...
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bioRxiv - Cell Biology 2022Quote: ... HUVEC were coated onto cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) and embedded in a fibrin matrix by combining 7µl of 50U/ml thrombin (T7201-500UN ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm pore-size polycarbonate filters (Whatman, GE Healthcare Europe GmbH, Milan, Italy) previously pre-coated with 2 μg/mL of fibronectin ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer with imidazole concertation brought up to 50 mM and suspension was applied on Flex-Column (420400-2510 ...
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bioRxiv - Cell Biology 2023Quote: ... in the 24-fraction setup using pI 3-10 IPG strips (GE Healthcare) for 50 kVh ...