Labshake search
Citations for GE Life Sciences :
51 - 100 of 2336 citations for tert Butyl 2 chloro 5H pyrrolo 3 4 b pyridine 6 7H carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2023Quote: To prepare activated magnetic beads 6.25mg of Sera-Mag SpeedBead hydrophilic carboxylate-modified paramagnetic particles (GE Life Sciences) were washed twice in MES buffer (500 mM 2-(N-morpholino)ethanesulfonic acid ...
-
bioRxiv - Plant Biology 2022Quote: ... 20 ug of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads, GE Life Sciences) were added to each sample in 50% ethanol ...
-
bioRxiv - Neuroscience 2023Quote: ... 20 μg of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads; GE Life Sciences) were added to each sample in 50% ethanol ...
-
bioRxiv - Cancer Biology 2023Quote: ... 20 μg of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads; GE Life Sciences) were added to each sample in 50% ethanol ...
-
bioRxiv - Neuroscience 2024Quote: ... 20 ug of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads, GE Life Sciences) were added to each sample in 50% ethanol ...
-
bioRxiv - Biochemistry 2024Quote: ... 20 μg of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads; GE Life Sciences) were added to each sample in 50% ethanol ...
-
bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein quality was assessed by SDS-PAGE and fractions containing RNase E were pooled and loaded onto a butyl sepharose column (GE Healthcare) equilibrated in high-salt buffer (50 mM Tris pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... The fractions eluted from anionic exchange column were incubated with ammonium sulfate to a final concentration of 1M and loaded on a Butyl Sepharose High Performance HiLoad16/10 (GE Healthcare) equilibrated with buffer C (25 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Indexed PCR products were purified using SPRI beads (1 mg/ml Sera-Mag carboxylate-modified magnetic particles (GE Healthcare), 38% PEG-8000 (w/v) ...
-
bioRxiv - Biochemistry 2024Quote: ... We used a 1:1 mixture of Sera-Mag Carboxylate-Modified Magnetic Beads (CMMBs; 65152105050250 and 45152105050250, GE Healthcare) to bind the solubilized peptides ...
-
bioRxiv - Microbiology 2024Quote: ... Solid-phase reversible immobilisation (SPRI) on carboxylated paramagnetic beads (SpeedBeed Magnetic Carboxylate Modified Particles 100 ml, Azide 0.05% 65152105050350, GE Healthcare) were functionalised for DNA-binding as described by [37] and stored at 4oC ...
-
bioRxiv - Biochemistry 2024Quote: ... 20 μg of beads (1:1 mixture of hydrophilic and hydrophobic Sera-Mag™ carboxylate-modified beads, GE Life Sciences) were added to each sample in 95% ACN ...
-
bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...
-
bioRxiv - Microbiology 2022Quote: ... The cleared lysate was incubated for 4 hours at 4°C using a rotating wheel with 3 mL of a GST bead slurry (GE Healthcare Life Sciences) pre-washed 3x with wash buffer (WB ...
-
bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
-
bioRxiv - Developmental Biology 2024Quote: ... The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3) and purified using glutathione Sepharose 4 B (GE Healthcare). An antibody against Ccrk was obtained by immunizing guinea pigs with purified GST-fused Ccrk.
-
bioRxiv - Immunology 2020Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification ...
-
bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
-
bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Reduced proteins were now purified from the lysate samples using SP3 magnetic beads (1:1 mixture of SpeedBead Magnetic Carboxylate Modified Particles 65152105050250 and 45152105050250, GE Healthcare). To enable protein binding to the beads ...
-
bioRxiv - Biochemistry 2020Quote: ... and Single-Pot Solid-Phase enhanced Sample Preparation (SP3) using paramagnetic beads (GEHE45152105050250 Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophilic, GE Healthcare) and GEHE65152105050250 Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophobic ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 µL of a 10 mg/mL mixture of hydrophilic and hydrophobic carboxylate coated paramagnetic beads (SeraMag Speed Beads, #44152105050250 and #24152105050250, GE Healthcare) were added to each sample ...
-
bioRxiv - Biochemistry 2021Quote: ... A 1:1 combination mix of two of magnetic carboxylate beads was used (Sera-Mag Speed beads, #45152105050250, #65152105050250, GE Healthcare). Beads were prepared fresh by rinsing with water three times prior to use at a stock concentration of 20 μg/μL ...
-
bioRxiv - Molecular Biology 2022Quote: ... equal volumes (125 μl containing 6250 µg) of two different kind of paramagnetic carboxylate modified particles (SpeedBeads 45152105050250 and 65152105050250; GE Healthcare) were mixed ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 µL of a 1:1 mix of two types of commercially available carboxylate beads (Sera-Mag Speed beads, 45152105050250, 65152105050250, GE Healthcare), and 60 µL ethanol ...
-
bioRxiv - Cell Biology 2023Quote: ... equal volumes (125 μl containing 6250 µg) of two different kinds of paramagnetic carboxylate modified particles (SpeedBeads 45152105050250 and 65152105050250; GE Healthcare) were mixed ...
-
bioRxiv - Cancer Biology 2023Quote: ... equal volumes (125 μl containing 6250 μg) of two different kind of paramagnetic carboxylate modified particles (SpeedBeads 45152105050250 and 65152105050250; GE Healthcare) were mixed ...
-
bioRxiv - Systems Biology 2024Quote: ... 50 µL of a 20 µg/µL SP3 bead stock (Sera-Mag SpeedBead carboxylate-modified magnetic particles; GE Healthcare Life Sciences) and 500 µL acetonitrile (ACN ...
-
bioRxiv - Cell Biology 2024Quote: ... equal volumes (125 μl containing 6250 µg) of two different kind of paramagnetic carboxylate modified particles (SpeedBeads 45152105050250 and 65152105050250; GE Healthcare) were mixed ...
-
bioRxiv - Biochemistry 2020Quote: ... The eluted protein sample was dialyzed against 20 mM Tris-HCl (pH 8.0) and 2 mM CaCl2 (buffer B) and applied to a Mono Q 10/100 GL column (GE Healthcare) equilibrated with buffer B ...
-
bioRxiv - Plant Biology 2021Quote: ... 100 mM DTT and 2% v/v IPG buffer pH 3– 10 (GE Healthcare) was applied to the strips ...
-
bioRxiv - Biochemistry 2023Quote: ... membranes were incubated for 2–3 minutes with ECL detection reagent (Amersham GE Healthcare), and were then developed using a ChemiDoc MP Imaging System (Bio-Rad) ...
-
bioRxiv - Biochemistry 2024Quote: ... 2) subtractive IMAC and 3) gel-filtration using Superdex 200 26/600 (GE Healthcare). Specially for SEC-MALS analysis ...
-
bioRxiv - Genetics 2020Quote: ... at 60 x magnification (Figs. 2 and 4) or DeltaVision microscope (GE Healthcare) at 100 x magnification (Fig ...
-
bioRxiv - Zoology 2020Quote: ... and 3500 V/2 mA/5 W for 6 h using a Multiphor II system (GE Healthcare). The IEF strip was then equilibrated for 15 min in 5 ml equilibration stock solution (6 M urea ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was supplemented with 4 mM CaCl2 and stirred for 2 h in 4°C with CaM-Sepharose beads (GE Healthcare), pre-equilibrated with binding buffer (50 mM Tris/HCl pH 7.2 ...
-
bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
-
bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
-
bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 1 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
-
bioRxiv - Systems Biology 2022Quote: ... was combined with 250 μg carboxylated paramagnetic beads (1:1 mix of hydrophiclic and hydrophobic Sera-Mag SpeedBead Carboxylate-Modified, GE Life Sciences) and 750 μl ethanol ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were then precipitated onto hydrophilic and hydrophobic Sera-Mag SpeedBeads (carboxylate-modified magnetic beads, hydrophilic: 45152105050250, hydrophobic: 65152105050250, GE Life Sciences) using 6-fold volume of ethanol ...
-
bioRxiv - Biophysics 2020Quote: ... followed by 2 CV of 100% IEX buffer B using an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare). To determine the point of elution of aSyn from the chromatography column protein fractions which were collected and monitored on absorption at 280 nm were ran on a 4-12% Bis-Tris gel (Invitrogen ...
-
bioRxiv - Biochemistry 2021Quote: ... using buffer B (GE Healthcare Biosciences) and a flow rate of 1 mL/min ...
-
bioRxiv - Biochemistry 2021Quote: ... Active HAP fractions eluting from 0.2–0.3 M KPi were pooled and separated on a Superose 6 XK 16/70 gel filtration column (130 ml, GE Healthcare) equilibrated with buffer D + 0.1 mM EDTA at 0.15 M KCl ...
-
bioRxiv - Plant Biology 2022Quote: ... purified PIN1 and sybody-21 were mixed together with a molar ratio of ∼1:3 and then applied to size-exclusion chromatography using a Superose 6 Increase column (GE Healthcare) in buffer containing 25 mM HEPES pH 7.4 ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) equilibrated in Dynein-lysis buffer and incubated for 3-4 h with rotation along the long axis of the tube ...
-
bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
-
bioRxiv - Biophysics 2021Quote: ... Reactions were incubated overnight at 4°C and resolved over a Superose 6 Increase 10/300 GL column (GE Healthcare) preequilibrated with assembly buffer ...