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Citations for GE Life Sciences :
51 - 100 of 147 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Bioengineering 2020Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Biochemistry 2021Quote: Fractions containing the dIG8-CC protein were then buffer-exchanged to buffer B (20mM Tris·HCl, 150 mM sodium chloride, pH 7.5) in a HiPrep 26/10 desalting column (GE Healthcare), and incubated overnight at 4°C with inhouse-produced His6-tagged TEV peptidase at a peptidase:substrate ratio of 1:20 (w/w ...
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bioRxiv - Biochemistry 2021Quote: ... flow rate 0.8 ml/min) or Superdex 200 Increase 5/150 (~3 ml, flow rate 0.45 ml/min) columns (GE Healthcare) were used ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of complement component C5 at 31.6 μM (5.96 mg/ml) was injected onto a Superdex 200 Increase 5/150 column (GE Healthcare) at a flow rate of 0.35 ml/min ...
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bioRxiv - Bioengineering 2020Quote: ... at room temperature with HBS-EP+ running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). IgGs were immobilized on CM5 chips (GE Healthcare # 29104988 ...
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bioRxiv - Biochemistry 2023Quote: ... The buffer was exchanged in assay buffer (10 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.1 % Tween-20) using PD10 gravity columns (GE Healthcare). The protein was concentrated using Amicon Ultra-0.5 10K Centrifugal Filter Devices (Merck Millipore) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Biochemistry 2023Quote: Dimer formation of non-farnesylated GBP1 was analyzed by analytical size-exclusion chromatography (Superdex 200 5/150, GE Healthcare) as described (Ince et al ...
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bioRxiv - Neuroscience 2023Quote: HMC3 cells were grown on 150 mm plates using EMEM (ATCC) supplemented with 10% bovine calf serum (GE Healthcare) and Pen/Strep (Wisent) ...
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bioRxiv - Bioengineering 2023Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Biophysics 2023Quote: ... Data collection was carried out at 20 °C using a Superdex200 Increase 5/150 analytical SEC column (GE Healthcare) equilibrated in the appropriate buffers (see Table S3 ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 mM NaCl at pH 7.6 and purified on a S75 10/300 sephadex size exclusion column (GE Healthcare). Appropriate fractions were collected and pooled ...
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bioRxiv - Synthetic Biology 2024Quote: ... with HBS-EP+ as the running buffer (10 mM HEPES at pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Proteins were immobilized on a CM5 chip (GE Healthcare #29104988 ...
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bioRxiv - Biophysics 2021Quote: ... The resulting VcParD2 fractions were pooled and dialyzed against 20 mM Tris pH 8.0 150 mM NaCl prior to a final SEC run on a Superdex 200 16:60 column (GE healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... was pre-equilibrated for 1.5 hrs at 4°C with Sup6-150 buffer without inhibitors or DTT on an AKTA Purifier system (GE Healthcare). 500 μL lysate (2 mg protein ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting filtrates were analyzed by size-exclusion column chromatography using Superose 6 Increase 10/150 GL (GE healthcare). Peak values at the retention time for the complex were plotted as a function of incubation temperature ...
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bioRxiv - Biochemistry 2020Quote: ... and the buffer was exchanged to 50 mM HEPES pH 7.0 containing 150 mM NaCl and 10 mM CaCl2 (Reaction Buffer) using a PD-10 desalting column (GE Healthcare). Aliquots containing 240 µg total protein were incubated at 37 C in the presence of 750 nM MMP2 or 9 or the Reaction Buffer alone for 2 hours ...
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bioRxiv - Biophysics 2021Quote: ... Pooled eluate fractions were adjusted to 150 mM NaCl and purified on a 5 ml HiTrap Heparin column (GE Healthcare) using a linear gradient from 150 mM NaCl to 1 M NaCl in buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... Purified Ycf1p NBD1 and NBD1-R were exchanged into the fluorescence buffer (20 mM Na3PO4, 2% glycerol, 150 mM NaCl, pH 7.3) by size exclusion chromatography (Superdex75, GE Healthcare). MgATP-bound and apo NBD1 and NBD1-R were generated by adding 2 mM MgATP or 2 mM EDTA ...
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bioRxiv - Plant Biology 2021Quote: ... in running buffer (20 mM Tris, pH 8, 150 mM NaCl, 1 mM DTT) supplemented with 0.05% of P20 surfactant (GE Healthcare). Anti-MBP murin monoclonal antibody (Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... and the peptides used for SPR was dissolved in HBS-EP+ (10 mM HEPES, 150 mM NaCl, 3 mM EDTA and 0.05% [v/v] surfactant P20 (GE Healthcare)) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted with a 20 ml linear gradient from 150 mM to 500 mM at a flow rate of 0.5 ml/min using an ÄKTA Pure instrument (GE Healthcare). The fractions containing the target proteins were pooled and concentrated to less than 0.5 ml using an Amicon Ultra concentrator (Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... Samples collected from IMAC were exchanged into size exclusion chromatography (SEC) buffer (20 mM Na2HPO4, 150 mM NaCl, pH 7.4) using either a HiPrep 26/10 desalting column (GE Healthcare) or through multiple rounds of concentration and dilution using an Amicon Ultra-15 filter unit with a 10 kDa molecular weight cut-off ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved protein was diluted with 20 mM Tris-HCl pH 7.5 to reach 150 mM NaCl and loaded onto pre-equilibrated HiTrap Q HP column (GE Healthcare). The column was washed with 200 mM NaCl Buffer R (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... MRPP1 and 2 formed a complex of ~150 kDa that was further purified by gel-filtration (Superdex 200, GE Healthcare). His6-Δ95 MRPP3 cDNA lacking the N-terminal disordered region was cloned into pMCSG7 vector and expressed and purified ...
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bioRxiv - Microbiology 2021Quote: ... and were eluted with CA loading buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM BME) using AKTAprime plus (GE Healthcare). CA monomer fractions were concentrated using Amicon Ultra-10K device (Merck Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... loaded complexes were further purified in a final size exclusion chromatography step (20 mM Tris-HCl pH 8.5, 150 mM NaCl) using a Superdex 200i column (GE Healthcare), before being used in down-stream applications.
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bioRxiv - Microbiology 2020Quote: ... Protein sample were injected into a size exclusion column (Superose™ 6 Increase 5/150 GL, GE Healthcare Bio-Sciences) previously equilibrated with corresponding buffer and eluted directly into the SAXS flow-through capillary cell at a flow rate of 0.2 ml/min ...
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bioRxiv - Biochemistry 2022Quote: ... Further purification was performed in column buffer (25 mM Tris HCl pH 8.0 and 150 mM NaCl) using HiLoad Superdex 200 gel-filtration chromatography (GE Healthcare). The peak fractions were collected ...
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bioRxiv - Biophysics 2022Quote: ... SEC–MALS was performed using 20 mM Tris pH 7.5 and 150 mM NaCl buffer equilibrated analytical Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) on a Shimadzu HPLC ...
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bioRxiv - Cell Biology 2022Quote: ... Data were collected following fractionation with an in-line size-exclusion chromatography column (Superdex 200 5/150 Increase, GE Healthcare,) pre-equilibrated in TBS pH 8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... monomeric C9 samples were passed through a Superdex 200 Increase 5/150 GL size-exclusion column (GE Healthcare Life Sciences) in 10 mM HEPESNaOH ...
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl.) supplemented with 2 mM CaCl2 and loaded onto a Superose6 Increase 10/300 GL column (GE Healthcare) integrated on a high-performance liquid chromatography (HPLC ...
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bioRxiv - Biochemistry 2022Quote: ... AcrB was then buffer exchanged to buffer C (50 mM sodium phosphate, 150 mM NaCl, 10 % Glycerol, pH 7.4) using a PD-10 desalting column (GE Healthcare) and flash frozen at -80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The BRCA1RING/BARD1RING and BRCA1RING/BARD1193 constructs were concentrated and further purified using a 120 mL Superdex 75 column on an AKTA FPLC system in SEC buffer (25 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 1 mM DTT; GE Healthcare). For the BRCA1RING/BARD1216 ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... the Ni-NTA eluate was applied to size exclusion chromatography on a Superose 6 Increase 5/150 column (GE Healthcare) in basic buffer supplemented with 0.003 % LMNG and 0.0003 % CHS.
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0) and further purified via size exclusion chromatography (SEC) in an S75 5/150 GL increase column (GE Healthcare). Protein collected from SEC was normalized to 1 μM where possible.
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bioRxiv - Biochemistry 2024Quote: ... The eluted purified protein was exchanged into 20 mM Tris buffer (150 mM NaCl, pH 8.0) and further purified by Akta Pure FPLC (GE Healthcare) SEC using a Superdex 75 increase 5/150 GL column ...
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl) and GST-fused proteins were immobilized on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-01) by incubating in a rotator at 4°C for 1 h ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified NanR at 10 mg mL-1 (340 μM) was injected (70 μL) onto an inline Superdex S200 5/150 Increase (GE Healthcare), equilibrated with buffer C ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: 150 μl sample was analyzed by Superose 6 Inc 10/300 column (Citiva, NY) equipped in ÄKTA purifier (GE healthcare, NY). The elution was conducted at 0.5 ml/min using the same buffer as used for sample at 4 °C
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bioRxiv - Biochemistry 2020Quote: ... The eluate was diluted two-fold in buffer E + 1 mM DTT + 150 mM NaCl and applied to a MonoS column (GE Healthcare) pre-equilibrated in buffer E + 150 mM NaCl + 1 mM DTT + 0.5 mM EDTA ...
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bioRxiv - Biochemistry 2021Quote: The oligomeric state of proteins as well as protein-protein and protein-tRNA interactions were analyzed by loading 50 μl samples on a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate using a Varian ProStar 335 system (Varian Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated in a vivaspin 100,000 MWCO and loaded onto a Superose 6 5/150 size exclusion chromatography column (GE Healthcare Lifescience) to remove thrombin and exchange the buffer to either 20 mm ...
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bioRxiv - Biochemistry 2020Quote: ... Complexes were purified by SEC on a Superdex 200 Increase GL 50/150 or a Superdex 200 Increase 10/300 GL column (GE Healthcare) and fractions containing CD4m-BG505-17b complexes were concentrated to 1.4-1.5mg/mL ...