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Citations for GE Life Sciences :
51 - 100 of 1172 citations for Water soluble CdTe CdSe ZnS quantum dots fluorescence λem 740 nm FWHM 60 nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... one of which was heating in a 65 °C water bath for 60 minutes and the second was using a Hi Trap QHP column (1 mL GE Healthcare, United States). When loading ...
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bioRxiv - Biochemistry 2020Quote: ... Absorbance was measured at 562 nm by the spectrophotometer Ultrospec 2100 UV/VIS (GE Healthcare™, Chicago, USA). The concentration was calculated by using a BSA standard (50 - 2000 µg/ml).
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bioRxiv - Neuroscience 2022Quote: ... NM- His was purified from the supernatant via IMAC using the ÄKTA pure protein purification system (GE Healthcare) together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Growth was monitored by optical density measurement at 600 nm using Ultrospec 10 Cell Density Meter (GE Healthcare). Samples for acyl-ACP ...
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bioRxiv - Cancer Biology 2022Quote: ... Ptch;p53 primary cell-lines (#9410) were transfected with 40 nM ON-TARGETplus mouse Yap1 siRNA (GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2023Quote: ... RNA yield and purity were verified by absorbance at 260/280 nm (NanoVUE spectrophotometer, GE Healthcare Life Sciences). 500 ng RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... The soluble portion was incubated with glutathione sepharose (GE healthcare) beads for 3-4 hours at 4°C and eluted with glutathione ...
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bioRxiv - Microbiology 2020Quote: ... Soluble proteins were purified using HisTrapTM Excel (GE Healthcare, Poland) column in buffer A with a linear gradient of 0-100% of 1 M imidazole in buffer A in 20 column volumes ...
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bioRxiv - Molecular Biology 2024Quote: ... soluble fractions were incubated with Glutathione-Sepharose beads (GE Healthcare) for 3 hours at 4°C ...
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bioRxiv - Genetics 2021Quote: ... incubated for 1 h at 65°C and dot-blotted on Amersham HybondTM-XL membrane (GE Healthcare). DNA was fixed by UV using a UV crosslinker BLX-254 (Analytik Jena) ...
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bioRxiv - Microbiology 2021Quote: ... the gels were blotted onto polyvinylidene difluoride (PVDF) membranes (Amersham™ Hybond™, 0.45 nm, GE HealthCare Life Science) at 60 mA for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... The dsRNAs concentrations were determined spectrophotometrically at 260 nm on a NanoVue™ Plus Spectrophotometer (GE Healthcare, Buckinghamshire, UK) and visualized in an agarose gel (1.5% w/v ...
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bioRxiv - Cell Biology 2020Quote: ... The concentration of the protein was estimated from the absorbance at 280 nm measured by a SimpliNano (GE healthcare) microvolume spectrophotometer with the extinction coefficient calculated by using the ExPASy ProtParam tool (51 ...
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bioRxiv - Immunology 2020Quote: ... 3-fold serial dilution (spanning a range of 1 μM to 1.4 nM) in HBS-EP buffer (GE healthcare). A high flow rate of 40 μL/min was used ...
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bioRxiv - Biophysics 2023Quote: ... 11 times through a NucleporeTM Track-Etch Membrane polycarbonate filter of pore size 400 nm (GE Healthcare Life Siences) and diluted to 4 mg/ml in reconstitution buffer ...
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bioRxiv - Cancer Biology 2020Quote: Soluble fractions were loaded onto an ÄKTA purification system (GE Healthcare) using an equilibrated HisTrap or GSTrap (both GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Soluble protein fraction was loaded onto Glutathione Sepharose FastFlow (GE Healthcare). GST-spDnmt2 was eluted from the column using 30 mM reduced glutathione ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction products were dot-blotted and UV cross-linked onto a Hybond N nylon membrane (GE Healthcare) followed by hybridized at 50 °C with end-labeled (CCCTAA)4 to detect C-circles or (TTAGGG)4 to detect G-circles.
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bioRxiv - Molecular Biology 2021Quote: ... The eluted DNA was dot-blotted and UV cross-linked onto a Hybond N nylon membrane (GE Healthcare) and hybridized with a telomere (or a tubulin ...
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bioRxiv - Microbiology 2020Quote: ... Raw data were sequentially taken by SI-super-resolution light path to collect 1.5 mm thick specimens in 125 nm increments in the z-axis with compatible immersion oils (Applied Precision). Standard OMX SI reconstruction parameters were then used in DeltaVision SoftWoRx Image Analysis Program to recon-struct the images ...
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bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
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bioRxiv - Cell Biology 2020Quote: siRNA-mediated knockdown in 786-O cells was carried out using 5-10 nM Dharmacon ON-TARGETplus siRNAs (GE Healthcare), diluted in siRNA buffer and mixed with DharmaFECT transfection reagent (1:50 in optiMEM reduced serum media) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05, GE Healthcare Dharmacon) or with siRNAs targeting PDK2 and PYCR1 (Dharmacon ...
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bioRxiv - Cancer Biology 2022Quote: ... and optical sections were collected at 200-nm intervals and processed using ratio deconvolution in softWoRx (GE Healthcare, version 6.5.2). Images were prepared for publication using FIJI (Version 2.1.0/1.53o) ...
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bioRxiv - Microbiology 2022Quote: Cell densities were determined based on the optical density at 600 nm (OD600) using an Ultraspec 2100 spectrophotometer (GE Healthcare) or a Tecan Infinite M1000 microplate reader ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were dissolved in PBS pH 7.4 at 42 μM concentration and filtered with a Whatman Anotop 10 filter (20 nm cutoff, GE Healthcare) before starting the measurements ...
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bioRxiv - Biophysics 2023Quote: ... proteoliposomes were extruded through a NucleporeTM Track-Etch Membrane polycarbonate filter with 400 nm pore size (GE Healthcare Life Siences). Reconstituted LeuT corresponding to 8 µg was treated with thrombin (0.5 unit ...
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bioRxiv - Developmental Biology 2022Quote: ... from which soluble forms were purified on glutathione-Sepharose beads (GE Healthcare). Recombinant C-terminally HA tagged Su(H ...
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bioRxiv - Cell Biology 2020Quote: ... The soluble fraction was bound to the GST Sepharose 4B (GE Healthcare) for 90 to 120 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was purified using an ÄKTAxpress chromatography system (GE Healthcare). The sample was purified firstly using a 1 mL HisTrap crude column (GE Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble fraction was loaded onto a nickel column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... 10/60 column (GE Healthcare). The monomeric peak fractions were collected ...
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bioRxiv - Neuroscience 2022Quote: ... The Biorad Bio-Dot apparatus was assembled according to the manufacturer’s protocol with Hybond N+ nylon membrane (GE Healthcare) on the top ...
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bioRxiv - Molecular Biology 2020Quote: ... connected to a ÄKTA Pure apparatus equipped with a UV 280 nm flow cell (all from GE Healthcare, Chicago, IL, USA). EV fractions were collected based on UV absorbance ...
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bioRxiv - Synthetic Biology 2020Quote: ... being the gels stained with SYBR safe and scanned with a Typhoon 9410 (two channels: 488/520 for SYBR; and 640/674 nm for Cy5) and analyzed with ImageQuant TL1 Software (GE Healthcare). Cy5-RNA concentration was determined in a Spark 10M multimode reader using a nanoquant plate (Tecan ...
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bioRxiv - Biochemistry 2021Quote: ... were flowed through with a concentration ranging from 0.05 to 100 nM and the data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: A mixture of 1 µM GST-NDP52 and 100 nM GFP-ULK1 complex was incubated with 10 µL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions with an absorbance at 512 nm were combined and loaded as described above onto a pre-equilibrated desalting column (HiPrep 26/10 Desalting, GE Healthcare) with a desalt buffer (200 mM sodium phosphate buffer at pH 7.5) ...
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bioRxiv - Bioengineering 2023Quote: ... Biomass concentrations were monitored using the cell growth quantifier (Scientific Bioprocessing) and determined manually by measuring the optical density at 600 nm with an Ultrospec 10 Cell Density Meter (GE Healthcare). The conversion factor of OD600 to cell dry weight (CDW ...
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bioRxiv - Microbiology 2024Quote: ... both extracts (Triton and SDS) were diluted 2:1 in 50 nM Tris-HCl and incubated with 50 µl Streptavidin Sepharose (GE Healthcare) overnight at 4°C while rotating ...
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bioRxiv - Microbiology 2020Quote: ... HyClone water (GE Healthcare) was used throughout.
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bioRxiv - Cell Biology 2022Quote: ... The peptide was diluted to 50 nM in running buffer (HBS-EP) and immobilized on a streptavidin sensor chip (sensor chip SA, GE Healthcare, USA). The analytes KEAP1 ...
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bioRxiv - Biophysics 2023Quote: Proteoliposomes were thawed and extruded through a NucleporeTM Track-Etch Membrane polycarbonate filter with 400 nm pore size (GE Healthcare Life Siences). Uptake was assayed in a 96-well setup in ultra-low attachment ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Biochemistry 2021Quote: ... the soluble fraction was applied to a HisTrap FF NiNTA column (GE Healthcare) and washed first with high-salt buffer B (2 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble extract cleared of cellular debris was incubated with IgG sephorose (GE Healthcare) for 2h at 4°C on a rotating wheel ...
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bioRxiv - Molecular Biology 2021Quote: ... The soluble protein was purified by absorption to Ni2+-NTA resin (GE Healthcare), washed with Ni-buffer A (20 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble fraction was loaded onto a nickel column (GE Healthcare, USA) pre-equilibrated with buffer A ...
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bioRxiv - Microbiology 2020Quote: ... soluble proteins were loaded onto a Ni-column (HisTrap FF Crude, GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 8.0 ...