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Citations for GE Life Sciences :
51 - 100 of 135 citations for Simvastatin EP Impurity A Sodium Salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0, 1M NaCl, 2mM MgCl2, 1mM ATP, 2mM beta-mercaptoethanol) on an ÅKTA system (GE Healthcare). Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... hippocampi were subjected to enzymatic digestion in Hanks′ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with Trypsin (1x ...
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bioRxiv - Biophysics 2022Quote: ... hSERINC3 was eluted in low salt buffer contain 2.5 mM desthiobiotin and then concentrated using a centrifugal filter (Vivaspin, 50 kDa MWCO (GE Healthcare)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Monomeric forms were purified from potential oligomeric species and salt using a Superdex 75 10/300 GL column (GE Healthcare) at a flowrate of 0.5 mL/min ...
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bioRxiv - Biophysics 2023Quote: ... Monomeric forms were purified from potential oligomeric species and salt using a Superdex 75 10/300 GL column (GE Healthcare) at a flow rate of 0.5 mL min−1 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified by salt gradient ion exchange chromatography using a Mono Q 5/50 GL column (GE healthcare) and Gradient buffers (20 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biophysics 2022Quote: ... and then purified from excess salt and potential oligomeric species using gel filtration on a size exclusion column (Superdex 75 10/300 GL, GE Healthcare) at a flow rate of 0.5 mL/min and eluted in 20 mM sodium phosphate 0.2 mM EDTA buffer (at pH 8.0) ...
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bioRxiv - Developmental Biology 2021Quote: ... Eluted protein was diluted to a final salt concentration of 200 mM NaCl and loaded onto a 5 mL HiTrap SP ion-exchange column (GE Healthcare).The protein was eluted in the same buffer with a salt gradient (200mM-1 M NaCl ...
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bioRxiv - Biophysics 2022Quote: ... and purified from any other oligomeric species and salts using a Superdex 75 10/300 GL column (GE Healthcare, Chicago, IL) at a flow rate of 0.5 ml/min in 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The eNOR containing fraction was eluted at around 250 mM salt and the eluted fraction was then loaded on a Chelating Sepharose (GE Healthcare) column ...
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bioRxiv - Biochemistry 2021Quote: ... bound protein was eluted using a gradient increasing the salt concentration to 500 mM KCl over 100 mL while collecting fractions (AEKTA prime FPLC system, GE Healthcare). Fractions were analyzed by SDS-PAGE and those containing pure full-length TwinStrep-UPF1 were collected ...
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bioRxiv - Genomics 2020Quote: ... Proteins were eluted with 3mL elution buffer (PBS, 250mM Imidazole) and excess salt was removed using the desalting column according to the manufacture’s protocol (GE Healthcare, 17085101). Subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... where 18 mL of blood was mixed with Hanks’ Balanced Salt Solution (HBSS, 14175095, GibcoTM) before being poured onto Ficoll-Paque Plus (GE17-1440-03, GE Healthcare). The layers were separated through centrifugation ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Biophysics 2022Quote: ... was diluted to a final salt concentration of 200 mM NaCl before loading onto a 1 mL heparin column (GE Healthcare) equilibrated in low-salt buffer (50 mM Tris pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... diluted with the same buffer devoid of NaCl to reach a final salt concentration of 40 mM and loaded on a HiSure Q 5 mL column (GE Healthcare). After washing with a 25 mM Na-HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... 20–40 g) and placed on ice in Petri dishes containing 1X Hank’s Balanced Salt Solution (HBSS, SH30588.02, GE Healthcare Life Sciences, US). Ganglia were minced using surgical micro scissors and incubated in a papain solution (32 μL papain (27.3 U/mg ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then diluted to a final salt concentration of low salt buffer conditions (100 mM KCl, 50 mM HEPES, 1 mM MgCl2) and bound to HiTrap SP FF (GE Healthcare, USA) anion exchange column ...
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bioRxiv - Cell Biology 2021Quote: ... Whole blood was diluted 1:1 with balanced salt solution at RT and layered onto Ficoll Paque PLUS (GE Healthcare, #GE17-1440-02) followed by centrifugation at 400 g for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... 1% saline-sodium citrate [SSC]) at 50 °C and exposed to a phosphor screen (GE Healthcare, USA) overnight ...
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bioRxiv - Microbiology 2022Quote: ... 0.3 M sodium citrate pH 7.0) and transferred to an Amersham Hybond-N+ membrane (GE Healthcare Life Sciences) by capillary action overnight ...
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bioRxiv - Biochemistry 2021Quote: Fractions containing the dIG8-CC protein were then buffer-exchanged to buffer B (20mM Tris·HCl, 150 mM sodium chloride, pH 7.5) in a HiPrep 26/10 desalting column (GE Healthcare), and incubated overnight at 4°C with inhouse-produced His6-tagged TEV peptidase at a peptidase:substrate ratio of 1:20 (w/w ...
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bioRxiv - Immunology 2021Quote: ... Samples were buffer exchanged into NMR buffer (20 mM sodium phosphate pH 7.0, 50 mM NaCl, 0.02% NaN3) using PD10 desalting columns (GE Healthcare). Samples were concentrated further to a volume of ~300 μL ...
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bioRxiv - Immunology 2020Quote: ... The trimer fraction was purified by size exclusion chromatography on a Superdex 200 10/300 GL column in TBS + 0.1% DDM + 0.003% sodium deoxycholate using an ÄKTA Pure 25L HPLC instrument (GE Healthcare) at a flow rate of 0.75 ml/min.
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bioRxiv - Cell Biology 2023Quote: ... as a model of pre-adipocytes [30] were cultured in DMEM (Nacalai, Osaka, Japan) supplemented with 10% fetal bovine serum (HyClone; GE Healthcare, Salt Lake City, UT, USA) and a mixture of 100 units/ml penicillin and 100 μg/ml streptomycin (Nacalai ...
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bioRxiv - Cell Biology 2024Quote: ... Public Health England, London, UK) were maintained in DMEM (Nacalai, Osaka, Japan) with 10% fetal bovine serum (FBS) (HyClone; GE Healthcare, Salt Lake City, UT, USA) and a mixture of 100 units/ml penicillin and 100 μg/ml streptomycin (P/S ...
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bioRxiv - Microbiology 2020Quote: ... Pure FasR and FasRF123A were dialysed against 10 mM sodium acetate pH 4.5 and coupled to a CM5 sensor chip using the Amine Coupling Kit (GE Healthcare). Micromolar concentrations of C20-CoA were dialysed against 25 mM Tris.HCl pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). After blocking with 8% skim milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2020Quote: ... α-Syn fibrils were formed by buffer exchange of purified monomers into fibril forming buffer (20mM Sodium phosphate, pH7.5) using a PD-10 column (GE Healthcare). The resulting α-Syn solution was passed through a 0.2 µm syringe filter ...
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bioRxiv - Developmental Biology 2020Quote: ... After the buffer was replaced with 20 mM sodium phosphate (pH 5.5) buffer containing 25 mM NaCl using a PD MiniTrap G-25 column (GE Healthcare), the digested sample was applied to a HiTrap Q HP column and every 1.5 ml was collected during the application and washing with the same buffer ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RBD was diluted to a concentration of 15 μg/mL with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units (RU) ...
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bioRxiv - Biochemistry 2020Quote: ... 250 μM TCEP and 20 mM sodium phosphate at pH 6.5 (ITC buffer) using a 5 mL HiTrap Desalting column (GE Healthcare). Likewise ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 ml of 20 µM purified Sup35NM were buffer exchanged into Fibril Forming Buffer (FFB - 20 mM sodium phosphate buffer pH 7.4, 50 mM NaCl) using a PD-10 column (GE Healthcare) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and the monomers were isolated in 20 mmol L-1 sodium phosphate pH 8.0 with 0.2 mmol L-1 EDTA by a Superdex 30 column 26/600 (GE Healthcare, UK). The concentration of monomeric Aβ42 was calculated with using an extinction coefficient of 1 424 M-1 cm-1 for (A280−A300) ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0) overnight at 4°C and further purified with Resource-S cation exchange column (GE Healthcare). Finally ...
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bioRxiv - Cell Biology 2024Quote: Samples were separated electrophoretically on a 12.5% sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE) and transferred to Polyvinylidene Difluoride (PVDF, GE Healthcare) membranes at 60 mA per membrane for 90 min in a semi-dry transfer unit (TE77X Hoefer) ...
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bioRxiv - Biochemistry 2023Quote: ... resolved by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electro-transferred on 0.45 μM nitrocellulose membranes (GE Healthcare). The membranes were incubated in blocking solution (5% skim milk in 0.05% Tween-20/Tris Buffered Saline ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped by adding 50 mM sodium succinate (pH 5.0) followed by buffer replacement by using Column PD 10 desalting column (GE Healthcare). Antibody SN-38 drug conjugates were prepared using purified antibody [30] ...
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bioRxiv - Immunology 2022Quote: ... Nb-Fc was diluted to a concentration of 5 μg/ml with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units ...
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bioRxiv - Biochemistry 2022Quote: ... AcrB was then buffer exchanged to buffer C (50 mM sodium phosphate, 150 mM NaCl, 10 % Glycerol, pH 7.4) using a PD-10 desalting column (GE Healthcare) and flash frozen at -80 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 6 µL of the reaction were diluted to 100 µL in 2x SSC (3 M NaCl, 0.3 M sodium citrate) and slot-blotted on a Hybond XL nylon membrane (GE Healthcare). For detection of ChIP samples ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Microbiology 2024Quote: ... The deacylated tRNA was ethanol precipitated and dissolved in monoQ buffer A (40 mM sodium phosphate buffer, pH 7.0) and separated on the 8-ml MonoQ column (10/100, GE Healthcare), using two-step linear gradient of buffer B (A with 1M NaCl) ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of lyophilized Aβ was resuspended in 700 µl GE buffer pH 8.5 (6 M guanidinium hydrochloride, 20 mM sodium phosphate) and loaded onto a Superdex 75 SEC column (GE Healthcare) equilibrated with aggregation buffer (20mM sodium phosphate ...
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bioRxiv - Plant Biology 2024Quote: ... Equal amounts of proteins were separated by sodium dodecylsulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) and transferred onto 0,45 μm nitrocellulose membranes (GE Healthcare) which were then probed with the following primary and secondary antibodies with the indicated dilutions ...
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bioRxiv - Biophysics 2020Quote: ... cysteine variants of α-synuclein were buffer exchanged into PBS or sodium phosphate buffer by use of P10 desalting columns packed with Sephadex G25 matrix (GE Healthcare). The protein was then incubated with an excess of Alexa-488 or Alexa-546 dyes with maleimide moieties (Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... media was diluted with an equal volume of Buffer A (500 mM NaCl, 20 mM sodium NaH2PO4, 20 mM imidazole) and loaded onto a 1 mL HisTrap column (GE Healthcare). The column was washed with Buffer A and the protein eluted with a gradient to Buffer B (500 mM NaCl ...
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bioRxiv - Bioengineering 2020Quote: A solution of 25 mg/L SC-SWCNTs and 2 mg/mL C16-PEG2k-ceramide in 1% sodium cholate was added to a 1 kDa molecular weight cutoff dialysis cartridge (GE Healthcare). The solution was dialyzed against water with multiple water exchanges for a week ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 50 μg of protein was separated on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto nitrocellulose membrane (GE Healthcare) with a Novex Gel Transfert Device (ThermoScientific ...