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Citations for GE Life Sciences :
51 - 100 of 5340 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The ARM domain was loaded on a Superose 6 Increase 10/300 (GE Healthcare) alone (at 2 mg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-GIGYF2 GYF domain was eluted from a Ni-NTA column (GE Healthcare) using 250 mM imidazole and further purified using a S200 10/600 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: Antibodies against CFTR were pre-incubated with protein-A or protein-G Sepharose beads (GE Healthcare) for 15 minutes at 4°C before adding protease-treated or non-treated lysates ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBS containing 0.1% polysorbate-20 and then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted with an imidazole gradient and fractions containing the target protein collected using an AKTA Prime system (GE Life Sciences). Protein quality was analysed by Coomassie gel analysis ...
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bioRxiv - Genetics 2022Quote: ... Antibody–protein interactions were revealed using chemiluminesence (RPN2108, GE Healthcare), and relative protein expression was quantified using ImageJ.
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bioRxiv - Neuroscience 2021Quote: ... or Protein G (monoclonal antibodies, GE Healthcare, 17-0618-01) agarose beads were washed with 1 ml of lysis buffer three times and then rotated with the lysates for 1 hour at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... for 10 min and blocked in PBS containing 2% bovine serum albumin (GE Healthcare) and 0.01% Tween 20 (National Diagnostics ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant containing the denaturing protein was loaded on HisTrap column (AKTA-prime; GE Healthcare) followed by washing with buffer C ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing desired protein was applied to either Glutathione-Sepharose resin (GE Healthcare Life Science) for GST purification or Ni-NTA Agarose resin (QIAGEN ...
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bioRxiv - Biophysics 2022Quote: ... The protein-containing supernatant was then applied to PD-10 Sephadex desalting column (GE Healthcare) to remove access of peptide/dye ...
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bioRxiv - Biophysics 2022Quote: ... The His6-α2I domain was purified using a Ni2+-charged HisTrap HP (GE Healthcare Life) and buffer exchanged to PBS ...
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bioRxiv - Biochemistry 2020Quote: ... HER2 domain IV was loaded into an anion-exchange chromatography column (HiTrap-Q, GE healthcare), and HER2 domain IV was collected from flow through.
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bioRxiv - Biochemistry 2021Quote: The Src SH2 domain superbinder was purified and immobilized on CNBr-activated Sepharose (GE Healthcare) in IAP buffer (50 mM MOPS-NaOH [pH7.2] ...
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bioRxiv - Genomics 2023Quote: ... each AP2 domain was previously cloned into the pGEX-4-T1 vector (GE Life Sciences) and transformed into BL21- CodonPlus(DE3)-RIL E ...
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bioRxiv - Genomics 2023Quote: ... except that antibody-bound protein complexes were isolated with protein A sepharose beads (GE Healthcare Life Sciences) washed with RIPA buffer (140mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... containing 1 mM 2-ME) and Superdex 200 Increase 10/300 GL column (GE Healthcare; 10 mM KPB [pH 7.0] containing 50 mM NaCl and 1 mM 2-ME) ...
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bioRxiv - Biochemistry 2020Quote: ... loaded in a gravity column containing 2 mL of pre-equilibrated Ni-sepharose (GE Healthcare), and incubated under slow rotation for 30 min at 8 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein input was detected using an anti-GST antibody (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies pre-adsorbed to protein G-sepharose beads (GE Healthcare) were added to the cell extract and the mixture incubated for 2 hr at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Plasma antibodies were purified using Protein A Sepharose resin (GE Healthcare), eluted from the resin with 0.1 M glycine at pH 2.5 and buffer exchanged into 1X PBS ...
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bioRxiv - Immunology 2022Quote: ... The undigested antibodies were removed with Protein A Sepharose (GE Healthcare) and the flow through was collected ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified via HiTrap Protein A HP columns (GE Healthcare) run at a flow rate of 0.5-1 mL/min on Äkta Start protein purification system (GE Healthcare) ...
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bioRxiv - Genetics 2020Quote: ... using native Msh5 antibody and Protein A Sepharose beads (GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Protein-containing fractions were pooled for desalting through a 5 mL HiTrap Desalting column (GE Healthcare). Desalted protein was dialyzed into a storage buffer (50 mM NaH2PO4 pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1500 µg protein extract containing GST-Gal1p was loaded on a GST SpinTrap column (GE Healthcare) and incubated for 1.5 hour at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The flow-through fraction containing target proteins were collected and purified with Superdex 75 (GE Healthcare) chromatography ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant containing soluble proteins were collected and allowed to flow through GST4B resin (GE Healthcare). After washing with three column volumes of sonification buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein-containing fractions were pooled and further purified on a Superdex 200 column (GE Healthcare), equilibrated and eluted in tubulin buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare) equilibrated in 200 mM potassium phosphate ...
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bioRxiv - Biochemistry 2023Quote: ... The protein-containing fractions were pooled and further purified on a Superdex 200 column (GE Healthcare), equilibrated ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing the target protein were pooled and loaded onto a Superdex S75 column (GE Healthcare) equilibrated in buffer containing 150 mM KCl ...
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bioRxiv - Developmental Biology 2021Quote: ... were coupled 2 h at RT to protein A Sepharose beads (GE Healthcare). Coupled beads were covalently crosslinked using dimethyl pimelimidate according to standard procedures ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using 2-D quant kit (GE healthcare, Cat# 80648356), by following manufacturer’ instruction ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were purified over 2 ml His-Trap FF column (GE Healthcare) with elution buffer containing 250 mM imidazole ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated with a rabbit polyclonal anti-HDA15 antibody and protein G Mag Sepharose beads (GE Healthcare) overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: ... and supernatants were then aliquoted into 2 tubes containing thiopropyl sepharose beads (GE Healthcare Life Sciences), with or without freshly-prepared HA (hydroxylamine 0.8 M ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing 2F2 or 2E10.E9 IgG was purified via Protein G affinity chromatography (GE Healthcare) and size-exclusion chromatography (Superose 6 Increase 10/300 GL ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the purified protein were pooled and desalted with PD midi Trap G-25 (GE Healthcare) using the spin protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing protein of interest were collected and desalted using PD-10 midi desalting columns (GE Healthcare). Protein of interest was eluted with the storage buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... supernatant containing VSG protein was loaded onto an anion-exchange column (Q-Sepharose Fast-Flow, GE Healthcare), which had been equilibrated with 10 mM phosphate buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Collected fractions containing membrane proteins were loaded onto a Superose 6 increase 10/300 column (GE Healthcare) and eluted with 30 mL of NCMN Buffer E ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant containing soluble proteins was collected and allowed to flow through Ni-NTA resin (GE Healthcare). After washing with three column volumes of sonification buffer ...
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Adaptation of the Romanomermis culicivorax CCA-adding enzyme to miniaturized armless tRNA substratesbioRxiv - Molecular Biology 2020Quote: ... Protein-containing fractions were combined and concentrated on Vivaspin 6 columns (15-30 kDa MWCO, GE Healthcare). Proteins were stored in 40 % glycerol (v/v ...
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bioRxiv - Microbiology 2020Quote: The heterologous proteins containing His-tags were purified via 1 ml Ni-NTA HisTrap columns (GE Healthcare). The cells were lysed in 50 ml lysis buffer containing 50 mM Na-phosphate buffer pH 8 ...
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bioRxiv - Cell Biology 2022Quote: ... Fractions containing GST-protein were pooled and loaded to a 6 mL Resource S column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Microbiology 2022Quote: ... supernatant containing VSG protein was loaded onto an anion-exchange column (Q-Sepharose Fast-Flow, GE Healthcare), which had been equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Genetics 2023Quote: ... Protein containing fractions were further purified by size exclusion chromatography using Superdex 200 10/300 (GE Healthcare) gel-filtration column in SEC buffer (20 mM HEPEs pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants containing soluble recombinant protein were pulled-down with Glutathione (GSH) Sepharose 4FastFlow (GE Healthcare, 17-5132) for 3h at 4°C with gently rocking ...
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bioRxiv - Microbiology 2024Quote: ... Collected supernatant containing the Fc fused nanobody was incubated overnight with protein-A Sepharose beads (GE Healthcare), after which proteins were purified using poly-prep chromatography columns (Bio-Rad) ...