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Citations for GE Life Sciences :
51 - 100 of 122 citations for PPM1L Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and purified using a Superdex Peptide 10/300 GL gel-filtration column (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... Crosslinked products were enriched by size-exclusion chromatography (Superdex Peptide, GE Healthcare Life Sciences) as described previously34 and fractions eluting between 0.9 and 1.4 mL were dried ...
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bioRxiv - Plant Biology 2019Quote: ... Peptides were purified via GST affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The GST tag was proteolytically cleaved by TEV-protease digestion ...
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bioRxiv - Biochemistry 2023Quote: ... Unreacted fluorescent peptide was removed using the Superdex 75 size exclusion column (GE Healthcare) in 50 mM TRIS pH 8 ...
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bioRxiv - Biophysics 2021Quote: ... and the excess small molecular was removed by Superdex peptide size-exclusion column (GE Healthcare) equilibrated in refolding buffer ...
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bioRxiv - Immunology 2019Quote: ... Bound biotinylated peptide was detected by addition of streptavidin-alkaline phosphatase conjugate (GE Healthcare, France) and 4-methylumbelliferyl phosphate substrate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Cross-linked peptides were enriched in a Superdex 30 Increase 3.2/300 column (GE Healthcare) equilibrated with 30% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Crosslinked peptides were enriched by size exclusion chromatography using an ÄKTAmicro chromatography system (GE Healthcare) equipped with a Superdex™ Peptide 3.2/30 column (column volume = 2.4 ml) ...
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bioRxiv - Microbiology 2021Quote: ... the peptide was purified with size-exclusion chromatography using a Superdex 75 column (GE Healthcare) equilibrated with gel filtration buffer (20 mM Na HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... The cargo tail peptides were isolated on gravity-flow column containing Ni-NTA agarose (GE Healthcare) followed by gel-filtration chromatography on Superdex Peptide 10/300 (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The eluent was then separated from the Cy3-peptide using a second heparin column (GE Healthcare) and polished using a MonoQ 5/50 GL (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies were diluted in blocking solution at 1:1000 (Anti-rabbit IgG: GE Healthcare Cat#NA934, RRID:AB_772206) and 1:2000 (Anti-mouse IgG ...
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bioRxiv - Synthetic Biology 2020Quote: ... the plate was blocked with 200 μl of a 2% (w/v) ECL Advance Blocking Reagent (GE Healthcare) solution in PBS-T (PBS supplemented with 0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... detection of antibodies was detected using the Amersham ECL Western Blotting Prime Blocking and Blotting Reagents (GE Healthcare).
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bioRxiv - Cancer Biology 2019Quote: ... 4 mg of trypsin digested peptides were separately processed on an AKTA Purifier UPLC system (GE Healthcare) fitted with an in-house column packed with 5-μm TiO2 beads (GL Sciences ...
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bioRxiv - Immunology 2021Quote: ... or other peptide–HLA-A2 ligand was immobilized on a streptavidin-coated BIAcore SA chip (GE Healthcare) at around 1000 resonance units (RU) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The radioactivity incorporated into each peptide was then determined using a Typhoon FLA 9500 phosphorimager (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... The radioactivity incorporated into each peptide was then determined using a Typhoon FLA 9500 phosphorimager (GE Healthcare) and quantified with the program ImageQuant (version 8.2 ...
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bioRxiv - Microbiology 2024Quote: ... The radioactivity incorporated into each peptide was then determined using a Typhoon FLA 9500 phosphorimager (GE Healthcare) and quantified with the program ImageQuant (version 8.2 ...
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bioRxiv - Microbiology 2020Quote: ... the filters were incubated with HRP-conjugated secondary antibodies in the respective blocking agent (NA931V and NA934V, GE Healthcare) for 1-2 h at RT or overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then blocked for 1 h with TBS-T 1% ECL Prime blocking reagent (GE Healthcare cat # RPN418) at RT on an orbital shaker and blotted with primary antibody for 1 h at RT with gentle agitation ...
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bioRxiv - Biophysics 2020Quote: ... This solution was purified by size exclusion chromatography using a superdex peptide column (10/300, GE Healthcare, Germany) in 20 mM phosphate pH 7.4 buffer containing 4M GdnCl ...
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bioRxiv - Molecular Biology 2021Quote: ... 2F2 Fab-peptide complexes were purified by size-exclusion chromatography (Superdex 200 Increase 10/300 GL, GE Healthcare), concentrated and diluted to 10 mg/mL ...
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bioRxiv - Physiology 2022Quote: ... The membrane was incubated for 1 hour at RT in 2% enhanced chemiluminiscence (ECL) prime blocking agent (GE Healthcare, RPN418). Membranes were incubated overnight at 4⁰C in blocking buffer (4% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBS containing 0.1% polysorbate-20 and then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... acetonitrile (ACN) and 0.1% (v/v) trifluoro acetic acid (TFA) using a Superdex Peptide PC 3.2/30 column (GE Healthcare). After evaporating to dryness and dissolving in 4% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... the sensor peptide was purified by size exclusion chromatography using the Superdex 75 10/300 GL column (GE Healthcare) and then stored at −80 °C before use.
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bioRxiv - Cancer Biology 2022Quote: ... peptides from whole cell samples were focused on immobilized pH gradient (IPG) gel strips (GE Healthcare, Waukesha, WI, USA) on a linear pH range of 3-10 ...
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bioRxiv - Bioengineering 2021Quote: ... Peptides were purified through a C18 prep column against an acetonitrile gradient on an AKTApure 25 FPLC (GE Healthcare) and confirmed by MALDI-TOF-MS (Bruker) ...
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bioRxiv - Microbiology 2022Quote: ... The solution containing the peptide was applied to a HiPrep SP FF 16/10 column (GE Healthcare Life Sciences) equilibrated with 20 mM sodium phosphate buffer at pH 3.9 ...
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bioRxiv - Plant Biology 2019Quote: ... pastoris and the peptide was purified from the culture medium using the Fast Protein Liquid Chromatography System (GE Healthcare) and Reverse Phase-High Performance Liquid Chromatography (RP-HPLC ...
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bioRxiv - Neuroscience 2022Quote: ... the Aβ42 peptide was recombinantly expressed in BL21*(DE3) pLysS Escherichia coli and purified with DEAE-Sepharose (GE Healthcare). To get rid of large Aβ42 aggregates ...
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bioRxiv - Plant Biology 2023Quote: ... the digested peptide filtrate was fractionated by strong cation exchange (SCX) chromatography using an AKTA Purifier system (GE Healthcare). The dried peptide mixture was reconstituted and acidified with 2 ml buffer A (10 mM KH2PO4 in 25%[v/v] of ACN ...
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bioRxiv - Cell Biology 2023Quote: ... The antibody was affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).
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bioRxiv - Biochemistry 2023Quote: ... Crosslinked peptides were desalted and reconstituted with SEC buffer [30% (v/v) ACN in 0.1% (v/v) TFA] and fractionated using a Superdex Peptide PC 3.2/30 column (GE Healthcare). Collected fractions were analysed by liquid chromatography-coupled tandem mass spectrometry (MS/MS ...
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bioRxiv - Molecular Biology 2023Quote: Interactions between the DMC1 protein and the synthetic BRCA2 peptides were characterized using a VP-ITC Calorimeter (GE Healthcare). The peptides tested were wild type and F2406A variant of the PhePP (F2s6 ...
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bioRxiv - Developmental Biology 2023Quote: ... The antibodies were affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked for 1 h at room temperature in TBS containing 4% Amersham ECL Prime Blocking Reagent (GE Healthcare) and 0.1% Tween 20 ...
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bioRxiv - Biophysics 2020Quote: ... Fixed cells were rinsed three times in warm PBS and incubated 30 min with a blocking solution containing 1% BSA (Bovine Serum Albumine, GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Biophysics 2023Quote: ... The fixed cells were rinsed three times in warm PBS and incubated for 30 min with a blocking solution containing 1% BSA (GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Biophysics 2021Quote: ... followed by buffer exchange in (50 mM HEPES, 150mM NaCl, pH 7.5) using a Superdex peptide size-exclusion column (GE Healthcare) to give the fluorescence labeled degron and Ub-degron.
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bioRxiv - Biochemistry 2019Quote: ... Crosslinked peptide fragments were enriched by SEC using a Superdex Peptide PC 3.2/30 column on an AKTA pure system (GE Healthcare) at a flow rate of 50 μL/min ...
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bioRxiv - Biochemistry 2019Quote: ... Resulting peptides after digestion were run in three independent Size-Exclusion Chromatography (SEC) runs on a Superdex Peptide 3.2/ 300 column (GE Healthcare) connected to an ÄKTAmicro FPLC system (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: Size exclusion chromatography was performed on a quaternary Agilent 1290 Infinity II UPLC system equipped with a SEC column (Superdex Peptide 3.2/300, GE healthcare). 40 μg of the eight protein mixture was loaded and separated at a flow rate of 0.05 ml/min with running buffer (30%ACN ...
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bioRxiv - Physiology 2022Quote: ... at a dilution of 1:2000 was done for 1 hour at RT in blocking buffer and detection was done using ECL detection kit (RPN2235, GE Healthcare, USA). Images were taken by the ImageQuant LAS500 (GE healthcare ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... to a polyvinylidene difluoride membrane and blocked in TBST buffer (TBS with 0.1% (v/v) Tween-20) supplemented with 1% ECL Advance Blocking Agent (GE Healthcare, Waukesha, WI) for 40 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... plasma or CSF probes were incubated in 25 μl for 1 hour at room temperature (diluted with PBS containing 5% Amersham ECL Blocking Agent, GE Healthcare RNP2125), followed by additional washing steps and incubation with an anti-mouse secondary antibody conjugated with the MSD Sulfo-tag for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were then washed in PBT and incubated with HRP conjugated-anti-rabbit IgG (GE Healthcare # NA9340, 1:5000 in blocking solution) at room temperature for 1 hour and washed twice in PBT ...
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bioRxiv - Biophysics 2021Quote: ... the FLAG-tagged Ubr1 was eluted with 1mg/mL FLAG peptide and further purified by a Superose 6 size-exclusion column (GE Healthcare) equilibrated in a buffer captaining 50 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... 3D11 Fab-NPNDx2 and 1210 Fab-NANP5 peptide complexes were purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare) and diluted to 25 µg/mL ...