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Citations for GE Life Sciences :
51 - 100 of 5049 citations for Mouse Serine threonine Protein Kinase 17B STK17B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... Protein bands were detected by enhanced chemiluminescence on X-ray film (ECL kit; GE Healthcare). To verify that the in-membrane hCALHM2 assembly corresponded in size to detergent extracted hCALHM2 ...
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bioRxiv - Plant Biology 2020Quote: ... The protein concentration was measured using 2-D Quant Kit (GE Healthcare, Piscataway, NJ, USA).
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bioRxiv - Neuroscience 2020Quote: Protein concentration in sarkosyl extracted samples was determined using the 2D-Quant Kit (GE Healthcare) following the manufacturer’s instructions and 12 µg of protein were loaded into 4-15% gradient gels (Biorad Miniprotean TGX ...
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bioRxiv - Biophysics 2021Quote: ... Standard proteins used were from the gel filtration calibration kit LMW and HMW (GE Healthcare) with the exception of Cryptococcus neoformans acyl-CoA binding protein (ACBP ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were visualized with an ECL western blotting detection kit (GE Healthcare, Chicago, IL, USA) through an Azure c600 gel imaging system and bands were quantified with an Azurespot software (Dublin ...
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bioRxiv - Biochemistry 2021Quote: ... [γ-32P] (Hartmann Analytic) using T4 polynucleotide kinase (Thermo Fischer) and subsequently purified using Illustra Microspin G25 columns (GE healthcare). Recombinant EB1 (0.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Neuroscience 2021Quote: ... cortices lysates were immunoprecipitated with anti-Bin1 or mouse IgG 16 h at 4 °C and then with 30 μl of protein G–Sepharose beads (GE Healthcare) for 3 h at 4°C ...
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Developmental Biology 2020Quote: ... Immuno-detected proteins were visualized by chemiluminescent photographic film exposure (ECL kit; GE Life Sciences, RPN2232) and digitally scanned using a GS-800 calibrated densitometer (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μg of each protein extract were concentrated using the 2D Clean-up kit (GE Healthcare) and then resuspended in 30 mM Tris pH 8.5 ...
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Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... Total protein contents were assayed using the 2-D Quant Kit (GE Healthcare, Little Chalfont, UK), measuring OD480 in a microplate reader (Tecan Group ...
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bioRxiv - Immunology 2020Quote: ... Total protein was quantified using the PlusOne 2D Quant Kit (GE Healthcare Europe GmbH, Freiburg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... and the protein concentrations were determined using a 2-D Quant Kit (GE Healthcare; Piscataway, USA).
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bioRxiv - Plant Biology 2023Quote: ... The protein concentration was determined with 2-D Quant Kit (GE Healthcare Life Sciences, Westborough, USA) using Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins of interest were revealed by chemiluminescence using the ECL WesternBlot detector kit (GE Healthcare) and the results were digitised in a UVITEC11Photo Imager (Ubitec Photoimager ...
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bioRxiv - Microbiology 2022Quote: ... end-labelled with [γ-32P ATP] (1000 Ci nmol-1) using T4 polynucleotide kinase and purified from free label by Sephadex G-50 spin columns (GE Healthcare). Increasing amounts of purified regulators were incubated with appropriately end-labelled DNA probes in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... and the phosphorylated kinase domain of FGFR1 was collected after size exclusion chromatography purification using Superdex 75 10/300 GL column (GE Healthcare). To prepare the dephosphorylated form ...
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bioRxiv - Cell Biology 2024Quote: ... The tagged proteins were detected using HRP conjugated sheep anti-mouse or anti-rabbit IgG antibody (1:10000, GE Healthcare, Uppsala, Sweden) and visualized using the Amersham ECL Western blot detection reagent (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... ELISA was performed as described2 using an HRP-conjugated anti-M13 monoclonal antibody (GE Healthcare) and a TMB substrate kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein bands were visualized using an Amersham ECL Advance Western Blotting Detection Kit (GE Healthcare, Germany).
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bioRxiv - Microbiology 2019Quote: Isolated proteins for each fraction and genotype were then precipitated with 2D Clean-Up kit (GE Healthcare), and dissolved in TS buffer (7M urea ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified by immobilized metal affinity chromatography using the His Trap Kit from GE Healthcare Life Science ...
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bioRxiv - Biochemistry 2022Quote: ... partition coefficients of standard proteins from the high-molecular-weight gel filtration calibration kit (GE Healthcare #28403842) were plotted against Log(MW ...
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bioRxiv - Microbiology 2021Quote: ... The approximate molecular weights of the loaded proteins were determined by molecular weight calibration kits (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... All columns were calibrated using protein standards from both high and low molecular weight kits (GE Healthcare).
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bioRxiv - Evolutionary Biology 2021Quote: ... the Sirh3-Venus fusion protein was detected with an ECL Prime Western Blotting Detection kit (GE Healthcare) using an anti-GFP antibody (MBL ...
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bioRxiv - Cell Biology 2021Quote: ... and protein bands were visualised via chemiluminescence using an ECL Plus Western blotting detection kit (GE Healthcare) and an LAS-4000 imager (Fujifilm) ...
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bioRxiv - Molecular Biology 2021Quote: ... Double stranded Oligonucleotides were end-labeled with T4 polynucleotide kinase in the presence of [γ-32P]ATP and purified using G-25 columns (GE Healthcare). To remove secondary structures present in them ...
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bioRxiv - Molecular Biology 2023Quote: ... The amount of [33Pγ]-tracer on the paper (signifying the kinase activity) was analyzed using a Typhoon FLA 700 IP PhosphorImager (GE Healthcare, Denmark), and related to the specific activity of the kinase reaction buffer ...
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Immunology 2020Quote: ... Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit, GE Healthcare, USA). In brief ...
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bioRxiv - Genomics 2021Quote: ... were 5’ end-labelled with [y-P32]-ATP by T4 polynucleotide kinase (New England Biosciences) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2019Quote: Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... protein G-Sepharose or protein A-Sepharose beads (GE Healthcare) for 2 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: Approximately 200 μL of normal mouse serum or anti-CD200 serum day 157 (after 4X immunizations) were purified using a protein G SpinTrap (GE Healthcare # 28-4083-47) according to the manufacturer’s instrutions ...
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bioRxiv - Biochemistry 2022Quote: ... Protein (GE Healthcare), according to the manufacturer’s specifications in protocol “E,” except that glutardialdehyde (25% w/v ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) and the protein concentration was determined using a 2-D Quant kit (GE Healthcare, Piscataway NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... We selected four proteins as molecular mass standards from the gel filtration calibration kit HMW (GE Healthcare, Uppsala, Sweden), i.e ...
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bioRxiv - Biochemistry 2019Quote: ... The size-exclusion column was calibrated using a set of standard proteins (Gel Filtration HMW Calibration Kit, GE Healthcare) in SEC buffer ...
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bioRxiv - Cancer Biology 2020Quote: We employed a protocol similar to that previously described.12 The broad-spectrum kinase inhibitor Ki-NET (CTx-0294885) was conjugated to NHS-activated Sepharose4 Fastflow resin (GE Healthcare, Little Chalfont, UK). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare) for immunoblotting ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
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bioRxiv - Bioengineering 2020Quote: ... Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare) affinity columns.
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bioRxiv - Cell Biology 2021Quote: ... and proteins were collected on protein G-Sepharose beads (GE Healthcare) for an additional 4 h at 4°C with gentle shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... a mixture of protein A and protein G sepharose (GE Healthcare life sciences ...
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bioRxiv - Molecular Biology 2023Quote: ... The sonicated proteins were pre-cleared with a 25μl protein A sepharose and protein G sepharose beads mixture (GE Healthcare) and the appropriate antibodies were added to the cleared extract and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified using HiTrap protein A column (GE Healthcare, 17040303).
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...