Labshake search
Citations for GE Life Sciences :
51 - 83 of 83 citations for Ketoconazole EP Impurity D since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... The protein concentration was measured using 2-D Quant Kit (GE Healthcare, Piscataway, NJ, USA).
-
Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... Total protein contents were assayed using the 2-D Quant Kit (GE Healthcare, Little Chalfont, UK), measuring OD480 in a microplate reader (Tecan Group ...
-
bioRxiv - Plant Biology 2021Quote: ... and the protein concentrations were determined using a 2-D Quant Kit (GE Healthcare; Piscataway, USA).
-
bioRxiv - Molecular Biology 2022Quote: ... siGENOME Control Pool Non-Targeting #2 (cat# D-001206-14-20) was purchased from GE Healthcare. siRNA transfection was performed using Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2023Quote: ... An adapter was 3-D printed to allow a GE 9LD transducer (GE Healthcare, Wauwatosa, WI) to be easily attached to or removed from the FASTER device (Fig ...
-
bioRxiv - Molecular Biology 2023Quote: ... the samples were concentrated using the 2-D Clean-Up Kit (80648451, GE Healthcare, Chicago, IL), reduced in 1 × Laemmli’s sample buffer (32.9 mM Tris HCl ...
-
bioRxiv - Plant Biology 2023Quote: ... The protein concentration was determined with 2-D Quant Kit (GE Healthcare Life Sciences, Westborough, USA) using Bovine Serum Albumin (BSA ...
-
bioRxiv - Microbiology 2021Quote: ... After incubation with secondary HRP-conjugated antibody (R&D) blots were developed using ECL reagent (GE Healthcare).
-
bioRxiv - Cancer Biology 2020Quote: ... The triarylmethyl EPR oxygen tracer OX063 (methyl-tris[8-carboxy-2,2,6,6-tetrakis[2-hydroxyethyl]benzo[1,2-d:4,5-d0]-bis[1,3]dithiol-4-yl]-trisodium salt) was obtained from GE Healthcare.
-
bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
-
bioRxiv - Biophysics 2024Quote: The yeast tRNAPhe from Sigma was labeled on its dihydrouridine (D) residues at the 16/17 positions using Cy3-hydrazide from GE Healthcare. This process involved first reducing the D residues with sodium borohydride (NaBH4 ...
-
bioRxiv - Cell Biology 2020Quote: ... pH 8.0) and the protein concentration was determined using a 2-D Quant kit (GE Healthcare, Piscataway NJ, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Duplicate 120 ml water samples were filtered serially through 2.7 μm Whatman GF/D (GE Healthcare, Little Chalfort, UK) and 0.22 μm Sterivex (Millipore ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05, GE Healthcare Dharmacon) or with siRNAs targeting PDK2 and PYCR1 (Dharmacon ...
-
bioRxiv - Plant Biology 2021Quote: ... The dried seeds were placed on two layers of glass microfiber filters (Whatman GF/D, GE Healthcare, Chicago, IL, USA) moistened with distilled water in a Petri dish in the dark at 23 °C for a week (conditioning) ...
-
bioRxiv - Neuroscience 2023Quote: ... Knockdown experiments involved the use of Accell non-targeting control siRNA (D-001910-01, GE Healthcare Dharmacon, Lafayette, CO, USA) and Accell mouse Rbfox1 siRNA (A-041929-13 ...
-
bioRxiv - Biochemistry 2022Quote: ... ON-TARGETplus SMART Pool Non-targeting (D-001810-10) and CHMP2A (L-020247-01) siRNAs were obtained from GE Healthcare Dharmacon ...
-
bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were precipitated the supernatant by addition of ammonium sulfate to 67% saturation and dissolved in buffer D and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer D ...
-
bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
-
bioRxiv - Microbiology 2023Quote: ... centrifuged 30 min at 2150g and concentrated/diafiltrated against loading buffer (D-PBS, 500 mM NaCl, 5 mM Imidazole) using the AKTA crossflow system (GE healthcare) supplemented with a 10KDa ...
-
bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
-
bioRxiv - Microbiology 2023Quote: ... M-003854-01-0005) and a “NonTargeting” (SMARTpool siGENOME NonTargeting, D-001210-04-20) control siRNAs were obtained from GE Healthcare Dharmacon (Lafayette ...
-
bioRxiv - Biochemistry 2023Quote: ... the supernatant clarified from the cell lysate after overnight induction by 0.5mM isopropylthio-β-D-1-galactopyranoside (IPTG) at 20 °C was applied to pre-equilibrated 5Lml HisTrap column (GE Healthcare). After thorough wash with binding buffer (20LmM Tris–HCl pHL7.4 ...
-
bioRxiv - Neuroscience 2024Quote: ... coli B21 Gold cells using 0.5 mM isopropyl thio-β-D-galactoside (IPTG) and purified on a NiNTA column (QIAGEN, ÄKTA Purifier, GE Healthcare) at 4 °C as described previously (2) ...
-
bioRxiv - Cell Biology 2021Quote: ... was used in the experiments shown in figure 3 and supplementary figure 6C while a pool of four scrambled siRNAs (#D-001206-13-05, GE Healthcare Dharmacon) was used in the experiments shown in figure 2 ...
-
bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... and analyzed (normalization, spot matching, expression analyses, and statistics) using Image Master 2-D Platinum 6 image analysis software (GE Healthcare, USA). Spots on the gel were identified and each spot was assigned a spot quantity (q) ...
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
-
bioRxiv - Neuroscience 2020Quote: We used a modified version of a previously described protocol (41). Briefly, we immobilized monoclonal 1D4 antibody (generously provided by D. Oprian, Brandeis University) onto Sepharose-4B (GE Healthcare Life Sciences). All steps were done in a darkroom under dim red-light illumination ...
-
bioRxiv - Microbiology 2020Quote: ... samples were immediately vacuum filtered through pre-weighed glass microfiber filters (Whatman GF/D, 2.7 µm pore size [GE Healthcare Life Sciences, Pittsburgh, PA]), filters were washed with at least 200 mL hot diH2O ...
-
bioRxiv - Cell Biology 2024Quote: ... Reduction of disulfide bonds and alkylation of cysteine residues was performed by incubating gel fragments in 10 mM 1,4-Dithio-D-threitol (GE Healthcare; cat. #17-1318-02) at 50°C for 1 hour followed by a light-protected incubation with 55 mM iodoacetamide (GE Healthcare ...
-
bioRxiv - Cell Biology 2020Quote: HESCs were grown in a six-well culture plate to 60%–70% confluence and transfected with 60 pmol of non-targeting siRNA (D-001810-10-05) or siRNAs targeting ACE2 (L-005755-00-0005) (GE Healthcare Dharmacon Inc., Lafayette, CO) in Lipofectamine 2000 reagent (Invitrogen Corporation ...