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Citations for GE Life Sciences :
51 - 100 of 4906 citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Hybond-N+ (GE Healthcare). A particular tRNA was hybridized with a corresponding antisense oligo DNA probe shown in Table S1 labeled with digoxigenin (DIG ...
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bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
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bioRxiv - Biochemistry 2022Quote: ... Protein standards (GE Healthcare Gel Filtration Calibration Kits) were used for comparison of elution volumes and estimation of theoretical molecular masses (Supplementary Figure 10 ...
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bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were resolved in a 6% polyacrylamide gel and transferred to a charged Hybond-N+ membrane (GE Healthcare). The membrane was cross-linked by UV (1200 mJ) ...
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bioRxiv - Molecular Biology 2022Quote: Reagents and kits used in this study were: Amersham Hybond N+ nylon membrane (RPN303B, GE healthcare), BCA kit (23225 ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare). Total protein was induced by isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Microbiology 2019Quote: ... Supernatant was filtered and N-glycosylated proteins isolated by affinity chromatography using Concavaline A columns (HiTrap Con A 4B, GE Healthcare) in AKTA FPLC (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
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bioRxiv - Microbiology 2021Quote: ... proteins were captured using an amine coupling kit (GE Healthcare), in which the carboxylmethyl dextran matrix of the sensor chip was activated by injection of a mixture of 0.2M 1-ethyl-3-[(3-dimethylamino ...
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bioRxiv - Plant Biology 2019Quote: ... Hybond-N+ (GE Healthcare, Chicago, USA), was placed on the MS plate with 1% agar ...
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bioRxiv - Microbiology 2021Quote: ... Hybond-N membranes (Amersham/GE Healthcare) were used for Southern blots (45) ...
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bioRxiv - Biochemistry 2021Quote: ... and Amersham Hybond-N+ (GE Healthcare) membranes were equilibrated in 1X membrane wash buffer (20 mM Tris-Cl ...
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bioRxiv - Neuroscience 2019Quote: ... P2 fractions were pre-cleared in NP-40 buffer for 4 hours and then incubated o/n at 4 °C with protein A-Sepharose beads (GE Healthcare, Milan, Italy) conjugated to 10 μg/ml of anti-Pin1 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2021Quote: ... Selected fractions containing the N protein were concentrated and loaded on two connected Superdex 200 10/300 GL size exclusion columns (GE Healthcare Life Sciences) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... was titrated into an A1 protein solution (22 μM except 50 μM with A1+OGly+N+C) in a MicroCal iTC200 (GE Healthcare Life Sciences). A priming injection of 0.4 μL (not included in data analysis ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
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bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: Proteins were precipitated using the 2D Clean-up Kit (GE Healthcare). The pellet was reconstituted in 50mM ammonium bicarbonate and digested with Trypsin (1/50 w/w ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined with 2-D Quant kit (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... Immunoreactive proteins were visualized using an ECL prime kit (GE HealthCare).
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bioRxiv - Microbiology 2020Quote: ... Total IgG was purified using Protein G gravitrap kit (GE healthcare). Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius) ...
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bioRxiv - Cell Biology 2022Quote: ... Detection of proteins was performed using the ECL kit (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... SEC columns were calibrated with a protein standard kit (GE Healthcare) containing ferritin (MW = 440 kDa ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected with ECL Advance kit (GE Healthcare,Poole UK).
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bioRxiv - Immunology 2021Quote: ... blotted onto Hybond-N+ membranes (GE Healthcare) and hybridized to biotin-labeled Sμ and σδ ...
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bioRxiv - Microbiology 2019Quote: ... using Hybond N membranes (Amersham/GE Healthcare) at a hybridization temperature of 45°C.
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bioRxiv - Immunology 2020Quote: ... N-hydroxysuccinimide (NHS)-Sepharose beads (GE Healthcare) were crosslinked with recombinant proteins according to the manufacturer’s instructions and were used for the purification of antibodies against DJ-1 protein ...
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bioRxiv - Developmental Biology 2020Quote: ... transferred onto Hybond N membranes (GE Healthcare), and probed with 32P-random-primed 1kb XmaI-EcoRV cDNA fragment corresponding to the exon 1-4 T-box region.
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bioRxiv - Molecular Biology 2023Quote: ... transferred to Hybond-N+ membrane (GE Healthcare), and hybridized over-night at 50 °C in ULTRA-Hyb Oligo Hybridization buffer (Invitrogen AM8663 ...
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bioRxiv - Zoology 2021Quote: ... Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare) as per manufacturer’s instructions (Figure A1 ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated using Quant kit (GE Healthcare, Chicago, Il, USA). Ten-microgram aliquots of proteins were diluted with 15 µl of 50 mM NH4HCO3 and reduced with 5.6 mM DTT for 5 min at 95°C ...
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bioRxiv - Bioengineering 2022Quote: ... gels were either silver stained (PlusOne Silver Staining Kit, Protein; GE Healthcare) or transferred onto nitrocellulose membranes for Western blot ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then precipitated using the 2D Clean Up Kit (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked and incubated with the antibody to detect neural Wiskott-Aldrich Syndrome protein (N-WASp; MilliporeSigma, Burlington, MA, USA) using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology) ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... RNAs were transferred to Hybond N+ (GE Healthcare) filters and transcripts of interest (SibA sRNA and proQ mRNA ...
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bioRxiv - Molecular Biology 2022Quote: ... and nylon membrane filters (Hybond N+, GE Healthcare). For analysis of target binding ...