Labshake search
Citations for GE Life Sciences :
51 - 100 of 6926 citations for Human Fibrinogen C Domain Containing Protein 1 FIBCD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: The RRM domain of RBFOX2 (amino acids 100-194) was cloned into the pGEX6P-1 expression vector (GE Healthcare, 28-9546-48) downstream of a GST-SBP tandem affinity tag ...
-
bioRxiv - Developmental Biology 2021Quote: ... were coupled overnight at 4°C to protein A Sepharose beads (GE Healthcare). Coupled beads were washed three times in EB buffer (50 mM Hepes ...
-
bioRxiv - Molecular Biology 2022Quote: ... overnight at 4°C in the presence of Protein A beads (GE Healthcare). ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems) ...
-
bioRxiv - Microbiology 2023Quote: ... the proteins were transferred onto a nitrocellulose membrane (Hybond-C Extra, GE Healthcare) and an anti-His-HRP conjugate antibody (Bio-Rad ...
-
bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
-
bioRxiv - Biophysics 2023Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
-
bioRxiv - Immunology 2022Quote: Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...
-
bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
-
bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
-
bioRxiv - Biophysics 2022Quote: ... Fractions containing target protein were desalted from imidazole using PD10 desalting column (GE Healthcare) and incubated with 50 μM ONO-5430608 and a His-tagged PreScission protease (homemade ...
-
bioRxiv - Cell Biology 2022Quote: ... The eluate containing recombinant proteins was passed through a PD-10 column (GE healthcare) to exchange buffer with Store Buffer (50 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Biophysics 2021Quote: ... Fractions containing the eluted protein were loaded onto a HiPrep desalting column (GE Healthcare) equilibrated with PBS ...
-
bioRxiv - Cell Biology 2019Quote: ... The supernatant fraction containing proteins were loaded on 5ml His-Trap column (GE Healthcare) and eluted with a linear gradient of elution buffer containing 40mM to 500mM Imidazole ...
-
bioRxiv - Biochemistry 2021Quote: ... Gel containing fluorescent protein samples were scanned with Typhoon FLA 9500 imager (GE Healthcare), using 473 nm blue LD laser/LBP (510LP ...
-
bioRxiv - Neuroscience 2023Quote: ... and supernatant containing GST-hnRNPH2 proteins added to Glutathione Sepharose 4B (GSH; GE Healthcare) beads ...
-
bioRxiv - Molecular Biology 2020Quote: ... The ARM domain was loaded on a Superose 6 Increase 10/300 (GE Healthcare) alone (at 2 mg/ml ...
-
bioRxiv - Biochemistry 2021Quote: ... The His6-GIGYF2 GYF domain was eluted from a Ni-NTA column (GE Healthcare) using 250 mM imidazole and further purified using a S200 10/600 column (GE Healthcare ...
-
bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
-
bioRxiv - Neuroscience 2022Quote: ... containing Cy3-conjugated streptavidin (1:1000) (GE Healthcare). To probe expression of different molecular markers in labeled neurons ...
-
bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
-
bioRxiv - Microbiology 2021Quote: ... proteins were blotted onto a nitrocellulose membrane (Hybond-C Extra, GE Healthcare, Munich, Germany) Ponceau S staining of the blotted membrane was performed to assure equal amounts of proteins ...
-
bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
-
bioRxiv - Biochemistry 2022Quote: ... Protein standards (GE Healthcare Gel Filtration Calibration Kits) were used for comparison of elution volumes and estimation of theoretical molecular masses (Supplementary Figure 10 ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted with an imidazole gradient and fractions containing the target protein collected using an AKTA Prime system (GE Life Sciences). Protein quality was analysed by Coomassie gel analysis ...
-
bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
-
bioRxiv - Microbiology 2021Quote: ... The supernatant containing the denaturing protein was loaded on HisTrap column (AKTA-prime; GE Healthcare) followed by washing with buffer C ...
-
bioRxiv - Biochemistry 2022Quote: ... Supernatant containing desired protein was applied to either Glutathione-Sepharose resin (GE Healthcare Life Science) for GST purification or Ni-NTA Agarose resin (QIAGEN ...
-
bioRxiv - Biophysics 2022Quote: ... The protein-containing supernatant was then applied to PD-10 Sephadex desalting column (GE Healthcare) to remove access of peptide/dye ...
-
bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
-
DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... DTX3L and Flag epitope for 1 h at 4°C before Protein G Sepharose beads (30 μL per reaction mixture; GE Healthcare #GE17-0618-01) were added for 12 h ...
-
bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
-
bioRxiv - Biophysics 2022Quote: ... The His6-α2I domain was purified using a Ni2+-charged HisTrap HP (GE Healthcare Life) and buffer exchanged to PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... HER2 domain IV was loaded into an anion-exchange chromatography column (HiTrap-Q, GE healthcare), and HER2 domain IV was collected from flow through.
-
bioRxiv - Biochemistry 2021Quote: The Src SH2 domain superbinder was purified and immobilized on CNBr-activated Sepharose (GE Healthcare) in IAP buffer (50 mM MOPS-NaOH [pH7.2] ...
-
bioRxiv - Genomics 2023Quote: ... each AP2 domain was previously cloned into the pGEX-4-T1 vector (GE Life Sciences) and transformed into BL21- CodonPlus(DE3)-RIL E ...
-
bioRxiv - Cell Biology 2020Quote: ... The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare). Total protein was induced by isopropyl-β-D-thiogalactopyranoside ...
-
bioRxiv - Molecular Biology 2021Quote: ... A second incubation overnight at 4°C with Protein G-magnetic Sepharose beads (GE Healthcare) was used to pull down protein:DNA complexes prior to washing in low salt (120mM NaCl ...
-
bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...
-
bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
-
bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
-
bioRxiv - Immunology 2022Quote: ... a 1:1 solution of Protein A Sepharose (GE Healthcare) and Protein G Sepharose (GE Healthcare ...
-
bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
-
bioRxiv - Microbiology 2021Quote: ... Protein-containing fractions were pooled for desalting through a 5 mL HiTrap Desalting column (GE Healthcare). Desalted protein was dialyzed into a storage buffer (50 mM NaH2PO4 pH 8.0 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1500 µg protein extract containing GST-Gal1p was loaded on a GST SpinTrap column (GE Healthcare) and incubated for 1.5 hour at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... The flow-through fraction containing target proteins were collected and purified with Superdex 75 (GE Healthcare) chromatography ...
-
bioRxiv - Plant Biology 2021Quote: ... The supernatant containing soluble proteins were collected and allowed to flow through GST4B resin (GE Healthcare). After washing with three column volumes of sonification buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... The protein-containing fractions were pooled and further purified on a Superdex 200 column (GE Healthcare), equilibrated and eluted in tubulin buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare) equilibrated in 200 mM potassium phosphate ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein-containing fractions were pooled and further purified on a Superdex 200 column (GE Healthcare), equilibrated ...