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Citations for GE Life Sciences :
51 - 90 of 90 citations for D Fructose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... beads were washed once in lysis buffer and rotated at 4ºC overnight in wash buffer containing 8 U/ml thrombin (GE healthcare) to remove the GST tag ...
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bioRxiv - Microbiology 2021Quote: ... After incubation with secondary HRP-conjugated antibody (R&D) blots were developed using ECL reagent (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... assay was performed to determine the binding affinities of aCPSF1 to poly-U-tract containing RNA on a BIAcore 8K instrument (GE Healthcare) as described previously (Zhang et al. ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were then resuspended in 650 µL of dialysis buffer and 100 U of Thrombin (GE Healthcare, #GE27-0846-01), to a final volume of 1 mL ...
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bioRxiv - Cancer Biology 2020Quote: ... The triarylmethyl EPR oxygen tracer OX063 (methyl-tris[8-carboxy-2,2,6,6-tetrakis[2-hydroxyethyl]benzo[1,2-d:4,5-d0]-bis[1,3]dithiol-4-yl]-trisodium salt) was obtained from GE Healthcare.
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bioRxiv - Biophysics 2024Quote: The yeast tRNAPhe from Sigma was labeled on its dihydrouridine (D) residues at the 16/17 positions using Cy3-hydrazide from GE Healthcare. This process involved first reducing the D residues with sodium borohydride (NaBH4 ...
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bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
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bioRxiv - Microbiology 2024Quote: ... the bacteria were resuspended at an OD600 = 0.5 U in three different nutritional culture conditions: (i) PBSG containing 25 mM HEPES (HyClone GE Healthcare Life Sciences), (ii ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) and the protein concentration was determined using a 2-D Quant kit (GE Healthcare, Piscataway NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Duplicate 120 ml water samples were filtered serially through 2.7 μm Whatman GF/D (GE Healthcare, Little Chalfort, UK) and 0.22 μm Sterivex (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... sfGFP-ACE2 was purified by an ion exchange column (HiTrap 5 ml Q FF anion exchange chromatography column; GE Healthcare, U. S. A.) eluted by a linear salt gradient from 10 to 1000 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-Scp160 antibodies (Gift of R. Jansen, U. Tuebingen) was performed using the Amersham ECL Western Blotting Detection Kit (GE Healthcare Life Sciences). Quantification of protein bands was performed using ImageJ software.
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bioRxiv - Microbiology 2021Quote: ... Cleavage of the GST tag to induce cleaved fusion proteins was performed using PreScission protease (10 U/mg of fusion protein, GE Healthcare, Uppsala, Sweden) or thrombin protease (10 U/mg of fusion protein ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05, GE Healthcare Dharmacon) or with siRNAs targeting PDK2 and PYCR1 (Dharmacon ...
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bioRxiv - Plant Biology 2021Quote: ... The dried seeds were placed on two layers of glass microfiber filters (Whatman GF/D, GE Healthcare, Chicago, IL, USA) moistened with distilled water in a Petri dish in the dark at 23 °C for a week (conditioning) ...
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bioRxiv - Biochemistry 2022Quote: ... ON-TARGETplus SMART Pool Non-targeting (D-001810-10) and CHMP2A (L-020247-01) siRNAs were obtained from GE Healthcare Dharmacon ...
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bioRxiv - Neuroscience 2023Quote: ... Knockdown experiments involved the use of Accell non-targeting control siRNA (D-001910-01, GE Healthcare Dharmacon, Lafayette, CO, USA) and Accell mouse Rbfox1 siRNA (A-041929-13 ...
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bioRxiv - Biochemistry 2021Quote: Three microliters of S-D614G in 1.2 mg/ml was mixed with 1.2 equivalent of RBD-chAb45 and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was further purified by size-exclusion chromatography (SEC) using a sizeexclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biophysics 2021Quote: ... The culture medium containing the secreted ACE2 was subsequently loaded onto an ion-exchange column (HiTrap 5 ml Q FF anion exchange chromatography column; GE Healthcare, U. S. A.), and subsequently eluted by a linear salt gradient ranging between 10 and 1000 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... The eluted S proteins were further purified by size-exclusion chromatography (SEC) using a Superose 6 increase 10/300 GL column (GE Healthcare, U. S. A.) in TBS buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Microbiology 2023Quote: ... Importin-α and Ran proteins lacking GST were prepared by cleavage with PreScission protease (20 U/mg of fusion protein; GE Healthcare, Uppsala, Sweden) at 4 °C for 12 h ...
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bioRxiv - Genomics 2022Quote: ... 0.5ml of antibiotic antimycotic solution (10000 lg/ml streptomycin, 10000 U/ml penicillin G, and 25 lg/ml amphotericin B, HyCloneTM, GE Healthcare Life Sciences, Amersham, UK) and 6ml of foetal calf serum were used to make lymphocyte short-term medium ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were precipitated the supernatant by addition of ammonium sulfate to 67% saturation and dissolved in buffer D and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer D ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant clarified from the cell lysate after overnight induction by 0.5mM isopropylthio-β-D-1-galactopyranoside (IPTG) at 20 °C was applied to pre-equilibrated 5Lml HisTrap column (GE Healthcare). After thorough wash with binding buffer (20LmM Tris–HCl pHL7.4 ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged 30 min at 2150g and concentrated/diafiltrated against loading buffer (D-PBS, 500 mM NaCl, 5 mM Imidazole) using the AKTA crossflow system (GE healthcare) supplemented with a 10KDa ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Microbiology 2023Quote: ... M-003854-01-0005) and a “NonTargeting” (SMARTpool siGENOME NonTargeting, D-001210-04-20) control siRNAs were obtained from GE Healthcare Dharmacon (Lafayette ...
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bioRxiv - Neuroscience 2024Quote: ... coli B21 Gold cells using 0.5 mM isopropyl thio-β-D-galactoside (IPTG) and purified on a NiNTA column (QIAGEN, ÄKTA Purifier, GE Healthcare) at 4 °C as described previously (2) ...
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bioRxiv - Cell Biology 2021Quote: ... was used in the experiments shown in figure 3 and supplementary figure 6C while a pool of four scrambled siRNAs (#D-001206-13-05, GE Healthcare Dharmacon) was used in the experiments shown in figure 2 ...
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bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... and analyzed (normalization, spot matching, expression analyses, and statistics) using Image Master 2-D Platinum 6 image analysis software (GE Healthcare, USA). Spots on the gel were identified and each spot was assigned a spot quantity (q) ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Cancer Biology 2024Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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bioRxiv - Neuroscience 2020Quote: We used a modified version of a previously described protocol (41). Briefly, we immobilized monoclonal 1D4 antibody (generously provided by D. Oprian, Brandeis University) onto Sepharose-4B (GE Healthcare Life Sciences). All steps were done in a darkroom under dim red-light illumination ...
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bioRxiv - Microbiology 2020Quote: ... samples were immediately vacuum filtered through pre-weighed glass microfiber filters (Whatman GF/D, 2.7 µm pore size [GE Healthcare Life Sciences, Pittsburgh, PA]), filters were washed with at least 200 mL hot diH2O ...
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bioRxiv - Cell Biology 2024Quote: ... Reduction of disulfide bonds and alkylation of cysteine residues was performed by incubating gel fragments in 10 mM 1,4-Dithio-D-threitol (GE Healthcare; cat. #17-1318-02) at 50°C for 1 hour followed by a light-protected incubation with 55 mM iodoacetamide (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: HESCs were grown in a six-well culture plate to 60%–70% confluence and transfected with 60 pmol of non-targeting siRNA (D-001810-10-05) or siRNAs targeting ACE2 (L-005755-00-0005) (GE Healthcare Dharmacon Inc., Lafayette, CO) in Lipofectamine 2000 reagent (Invitrogen Corporation ...