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Citations for GE Life Sciences :
51 - 100 of 114 citations for Cyanine5 NHS ester minimaldye since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... and mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody ...
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bioRxiv - Biophysics 2023Quote: ... and mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody ...
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bioRxiv - Immunology 2020Quote: ... N-hydroxysuccinimide (NHS) and ethanolamine (H2N(CH2)2OH) were purchased from GE Healthcare. HpARI ...
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bioRxiv - Biochemistry 2024Quote: ... IAC was performed on HiTrap NHS-activated HP 5-mL columns (GE Healthcare) coupled with 2 to 3 mg/mL of either MNB4 (an anti-PTX3 monoclonal antibody recognising an epitope in the N-terminal domain that was used to purify PTX3 ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.4 and applied to a 5 mL Hi-Trap NHS column (GE Healthcare), which contained 20 mg of immobilised E141A H164A OmCI protein ...
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bioRxiv - Biochemistry 2020Quote: ... 1 ml of NHS-Activated Sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) was washed with 10-15 column-volumes of 1 mM HCl ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) coupled to a GFP nanobody expressed and purified by published protocols45 and incubated on a rocker for 1.5 hours ...
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bioRxiv - Immunology 2021Quote: ... CM5 chip surface was activated by injecting a solution of EDC/NHS (GE Healthcare). Mouse anti-human IgG (Fc ...
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bioRxiv - Neuroscience 2024Quote: ... was bound to a 1 ml HiTrap® NHS- activated HP column (GE Healthcare) according to manufacturer’s guide ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) coupled to an anti-GFP nanobody expressed and purified by published protocols2 and incubated on a rocker for 2 hours ...
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bioRxiv - Microbiology 2021Quote: PBMCs were extracted from Leukocyte cones from healthy donors (NHS) via Ficoll (17544202, GE Healthcare) density gradient centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... NHS-activated Sepharose 4 Fast Flow and Q-Sepharose Fast Flow was from GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2020Quote: ... 20 mM EDTA and applied to a 5 mL Hi-Trap NHS column (GE Healthcare), which contained 20 mg of E141A H164A OmCI protein ...
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bioRxiv - Molecular Biology 2020Quote: ... eDNA was captured onto 0.45 μm mixed cellulose ester membranes (MCE, 47 mm diameter, Whatman, GE Healthcare) using a vacuum-pump and Nalgene™ filtration units ...
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bioRxiv - Biochemistry 2022Quote: ... The in-house resin is prepared by coupling N-hydroxysuccinimide (NHS)-Activated Sepharose 4 (GE Healthcare) with 2-(Pyridyldithio ...
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bioRxiv - Molecular Biology 2021Quote: ... The 25 μL sample was then fluorescently labeled with 10 μg Cy3-succinimidyl-ester (GE Healthcare, Buckinghamshire, UK) for 1 h at room temperature in the dark ...
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bioRxiv - Microbiology 2020Quote: ... The J2 were then filtered onto 47mm diameter and 0.45µm pore size cellulose ester filters (GE Healthcare Whatman). All samples were kept at -80 °C until DNA extraction ...
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bioRxiv - Biochemistry 2020Quote: ... DBCO beads were synthesized in house: NHS-activated Sepharose™ fast flow (#17-0906-01, GE Healthcare) was incubated to varying concentrations of dibenzocyclooctyne-amine (DBCO-amine ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified on Fy-4 immobilized on a HiTrap NHS- activated HP column (GE Healthcare). Antibodies were eluted using Pierce Gentle Ag/Ab Elution Buffer (ThermoFisher).
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bioRxiv - Immunology 2021Quote: ... each ectodomain variant was chemically cross-linked to N-hydroxysuccinimide (NHS)-activated Sepharose beads (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Ligand immobilization was done through amine coupling to an NHS-EDC activated CM5 chip (GE HealthCare, Uppsala, Sweden). CR6261 or CR9114 were immobilized at 1000 RUs in 10 mM Sodium Acetate buffer (pH 4.5 ...
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bioRxiv - Immunology 2022Quote: ... Competition was studied using pH1HA10-Foldon immobilized on an NHS-EDC activated CM5 chip (GE HealthCare, Uppsala, Sweden) through amine coupling until 1500 RUs were achieved and subsequently probing with two analytes without an intermittent dissociation step ...
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bioRxiv - Microbiology 2021Quote: ... The excess DBCO-NHS was removed using Sephadex G-25 desalting columns (GE Healthcare Japan Co., Tokyo, Japan). The DBCO-labeled lectin product (100 μg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... was immobilised on a CM5 chip through amine conjugation using NHS/EDC coupling using a Biacore X100 (GE Healthcare). Samples were run at 30 μL/min with an injection time of 60 s and a dissociation of 200 s ...
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bioRxiv - Microbiology 2021Quote: ... And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... hTau and mTau were immobilised onto an amine-coupling activated (NHS/EDC) CM5 Series S Sensor Chip (GE Healthcare) with a contact time of 180 s at 5 µL/min flow rate and at 25°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Water was filtered using vacuum-pumps coupled with Nalgene™ units and DNA was captured onto 0.45 μm mixed cellulose ester filters (47 mm diameter, Whatman, GE Healthcare). Generally ...
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bioRxiv - Biophysics 2020Quote: ... which is complementary to the tracrRNA 5’-end was labeled with ATTO647N-NHS (1:60 DNA:dye molar ratio) and purified using G-25 micro spin column (Illustra, GE Healthcare). The measured labeling efficiency of ATTO647N-oligonucleotide was >80% ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Affinity purification of TaBGC1-specific antibodies from the antiserum was performed with a HiTrap NHS-Activated HP column (GE Healthcare), conjugated to TaBGC1 recombinant protein.
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bioRxiv - Cell Biology 2020Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Biochemistry 2020Quote: ... Ab Spin Trap columns (immobilized protein G) and NHS-activated sepharose® 4 fast flow were purchased from GE Healthcare Life Sciences (Buckinghamshire ...
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bioRxiv - Genomics 2021Quote: ... The excess DBCO-NHS was also removed by using Sephadex G-25 desalting columns (GE Healthcare Japan Co., Tokyo, Japan). The resulting DBCO-labeled lectin was then incubated at 4 °C with ten-times the molar amount of 5’-azide-modified DNA oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2023Quote: ... rabbit serums from day 67 post-inoculation were purified using a 1 ml HiTrap NHS-Activated HP column (GE Healthcare) loaded with the corresponding antigen according to manufacturer instructions ...
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bioRxiv - Biophysics 2020Quote: ... Unconjugated TFP ester dyes were separated from labeled proteins using HiTrap Desalting columns with Sephadex G-25 resin (GE Healthcare Life Sciences). Alexa Fluor™ 647 C2 Maleimide (A20347 ...
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bioRxiv - Plant Biology 2020Quote: ... 9 mL of filtered immune serum were purified against the soluble part of the recombinant protein (10 mg) covalently coupled to 1mL HiTrap NHS-activated HP resin (GE Healthcare) according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...
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bioRxiv - Immunology 2021Quote: ... PPACK-thrombin or fibrinogen in acetate buffer pH4.5 was immobilized to a chip surface (1000 or 10000 RU) using NHS/EDC-amine coupling and residual binding sites blocked by 1M ethanolamine (GE Healthcare). FH (0.5-62nM ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µg of a 5’ amino-modified DNA oligonucleotide complementary to the target tRNA (IDT) was covalently coupled to a HiTrapTM NHS-activated HP column (1 mL, GE Healthcare). For isolation of full-length tRNAs ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at room temperature for 1 hour prior to removal of unreacted Biotin-NHS by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Biochemistry 2021Quote: ... SARM1-dN was pulled down by BC2 nanobody(Bruce et al., 2017) conjugated beads which were prepared by conjugating BC2 nanobody to NHS-beads (GE Healthcare). The purified SARM1-dN ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-NPR1 antibodies were purified from antisera by affinity chromatography using a tNPR1-His-immobilized column (Hitrap NHS-activated HP; GE Healthcare). To prepare for the tNPR1-His-immobilized column ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was then mixed with GFP resin which was produced by crosslinking nanobody71 to NHS-Activated Sepharose 4 Fast Flow beads (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 80 µg of a 5' amino-modified DNA oligonucleotide (Table S1) complementary to target tRNA (IDT) was covalently coupled to a HiTrapTM NHS-activated HP column (1 ml, GE Healthcare). Binding to the NHS column was performed by recirculation of the small RNA fraction over the complementary NHS columun for 3 hours at 65°C ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants of cell lysate were incubated with either pre-equilibrated GFP nanobody-conjugated NHS-Activated Sepharose 4 Fast Flow Agarose (GE Healthcare) or Pierce Protein A Agarose (Invitrogen/Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... SOSIP proteins were isolated from cell supernatants using a 2G12 immunoaffinity column made by covalently coupling 2G12 IgG monomer to an NHS-activated Sepharose column (GE Healthcare). Protein was eluted with 3M MgCl2 followed by buffer exchange into TBS ...
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were biotinylated using EZ-Link NHS-LC-LC-biotin (Pierce) and conjugated to one of several fluorophores - Cy5 Monoreactive dye (GE Healthcare), AlexaFluor 405 or AlexaFluor 647 NHS esters (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...
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bioRxiv - Immunology 2020Quote: ... Secreted Env trimers were isolated using PGT145 immunoaffinity chromatography by covalently coupling PGT145 IgG monomer to an activated-NHS Sepharose column (GE Healthcare) as previously described (81) ...
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bioRxiv - Immunology 2021Quote: ... to prevent activation of the alternative complement pathway and complement consumption) and anti-FH mAb (35H9) on Hi-Trap HP-NHS columns (GE Healthcare). FH protein was purified from EDTA-plasma using an anti-FH mAb (35H9 ...