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Citations for GE Life Sciences :
51 - 100 of 2210 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The membrane was incubated first with primary antibodies for 24 h at 4°C and then with horseradish peroxidase– conjugated secondary antibodies (GE Healthcare) for 1 h at room temperature ...
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bioRxiv - Genetics 2021Quote: ... and then incubated with primary antibodies overnight at 4°C and then again with horseradish peroxidase–linked secondary antibodies (GE Healthcare) in PBST with 5% dry milk for 1 hour at RT ...
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bioRxiv - Biochemistry 2021Quote: ... The α-domains of the proteins were purified to homogeneity by size exclusion chromatography (Superdex 75 GE Healthcare) equilibrated in 25 mM HEPES pH 7 ...
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bioRxiv - Synthetic Biology 2020Quote: The DNA fragment encoding the PreS1 domain (residues 1-56) was cloned into the pGEX-2T (GE Healthcare) plasmid ...
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bioRxiv - Biophysics 2019Quote: The purified Tom1 VHS domain was loaded onto an FPLC system using a Superdex 75 column (GE Healthcare) equilibrated with 50 mM Tris-HCl (pH 7.3) ...
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bioRxiv - Cell Biology 2023Quote: ... the EfLigA domain of NMoRI was further purified by gel filtration using a Superdex-200 column (GE Healthcare) equilibrated against buffer (25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was incubated (2 h, 4 °C) with 4.0 mL of Glutathione Sepharose 4B resin (GE Healthcare) that had been pre-equilibrated in the same buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Gels were dried at 80 °C for 2 hrs and covered with phosphor imaging plate (GE Healthcare) overnight after RNA fixation in RNA fixation buffer (40% ethanol ...
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bioRxiv - Microbiology 2023Quote: Antibody binding properties were analysed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... KIX domain was cleaved from GST by incubation of resin-bound GST-KIX with 160 U thrombin (GE Healthcare) overnight at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... VSG13 N-terminal domain was purified by gel filtration on a HiLoad 16/600 Superdex 200pg column (GE Healthcare) in 50 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant catalytic domain of PknB was purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) as previously described5.
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bioRxiv - Biochemistry 2023Quote: ... Various Prp40 domains fused with an N-terminal Flag tag were cloned into pGEX-6p-1 vector (GE Healthcare) and purified from E.coli as GST fusion proteins ...
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bioRxiv - Biochemistry 2021Quote: ... dried for 2 h at 65°C and radiolabelled products visualised using a Typhoon FLA-700 (GE Healthcare) following exposure to a phosphorimaging plate for 24-72 h.
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bioRxiv - Molecular Biology 2022Quote: ... 93-207 Bcl-2) with a C-terminal 6His tag and purified using Ni2+ IMAC (GE Healthcare, Fastflow). Similarly ...
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bioRxiv - Biochemistry 2021Quote: Single cysteine mutants of ColE9 or the ColE9 DNase domain were desalted on a 5 ml HiTrap column (GE Healthcare) equilibrated with binding buffer (20 mM potassium phosphate pH 7 ...
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bioRxiv - Microbiology 2020Quote: ... The VSGsur N-terminal domain was purified by gel filtration on a HiLoad 16/600 Superdex 200pg column (GE Healthcare) in 10 mM Tris.Cl ...
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bioRxiv - Molecular Biology 2020Quote: ... MnCl2 or CaCl2was titrated into a sample cell containing 0.1 mM catalytic domain of the g12777 protein using an iTC200 calorimeter (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: Purified His-tagged proteins anchor domain (2.1 mg/ml) was injected onto a Superdex 75 Increase 10/300 GL size exclusion column (GE Healthcare), equilibrated with 20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences) directly to the glutathione beads followed by incubation overnight at 4°C to obtain His-tagged RhoGAP ...
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bioRxiv - Immunology 2021Quote: ... The head domain was purified from the supernatants by nickel affinity chromatography with HisTrap Excel columns (GE Healthcare Life Sciences), and H5.31 and H5.28 Fabs were purified with CaptureSelect IgG-CH1 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... The purified EGFR recombinant extracellular domain was first coupled to the surface of a CM5 sensor chip (GE Healthcare Biosciences)) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 ml of purified WT SAM domain at 9.5 mg/ml was loaded onto a Superdex 75 16/60 gel filtration column (GE Healthcare) at 1 ml/min pre-equilibrated in buffer D ...
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bioRxiv - Molecular Biology 2019Quote: ... or Ni-NTA-Agarose (GE healthcare, His-B-MYB). Lysates of HeLa cells expressing HA-tagged B-MYB constructs were incubated with 5 mg immobilized GST or GST-WW1/2-YAP for 3 hours at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... Sera-Mag Speed Beads A and B (Ge Healthcare) were vortexed until the pellet was dissolved ...
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bioRxiv - Plant Biology 2021Quote: ... Two 2 ml aliquots were collected using GF/C glass microfiber filters (20 mm; Whatman, GE Healthcare Life Sciences), transferred to glass tubes ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 2 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... The extracted mononucleosome fraction was incubated for 2 h at 4°C with Protein G Sepharose beads (GE Healthcare), which were preincubated with rabbit polyclonal anti–chicken CENP-A antibody64 ...
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bioRxiv - Biochemistry 2020Quote: The antibodies binding properties were analyzed at 25 °C on a Biacore ™ 8K instrument (GE Healthcare) using 10mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) containing 5% skim milk (Meiji) and subjected to primary antibody for 2 h and HRP-conjugated secondary antibody (GE Healthcare) for 30 min ...
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bioRxiv - Bioengineering 2022Quote: ... the receptor fragment MET567 comprising the N-terminal SEMA domain and the cysteine-rich/PSI domain was immobilized at low-density (515 RU) using amine-coupling on a CM5 sensor chip (GE Healthcare). A blank inactivated reference channel was used to correct for non-specific binding ...
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bioRxiv - Biochemistry 2020Quote: ... REC3 domain (WT and R691A mutant) were further purified by size-exclusion chromatography on a Superdex 200 16/60 column (GE Healthcare) in 20 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... The methylated VSG13 N-terminal domain was purified by gel filtration on a Superdex 200 Increase 10/300 GL (GE Healthcare) in 10 mM Tris.Cl ...
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bioRxiv - Microbiology 2021Quote: GST alone and GST-tagged WWOX WW1 and WW2 domains fusion protein were expressed in BL-21 cells and subsequently purified and conjugated to glutathione (GSH) beads (GE HEALTHCARE). HEK293T cells were transfected with eVP40-WT ...
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bioRxiv - Cell Biology 2022Quote: GST or GST-tagged β’-COP (residues 1-604) or Glo3 constructs (GAP domain, BoCCS region, GRM region) were immobilized on glutathione sepharose resin (GE Healthcare) for one hour on ice ...
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bioRxiv - Neuroscience 2019Quote: SPR biosensor analysis was used to verify binding between peptides and the PDZ2 domain of FRMPD2 protein using a Biacore 8K instrument (GE Healthcare), as previously described(Zhang et al. ...
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bioRxiv - Immunology 2020Quote: ... the surface was saturated with two sequential injections of a 20 μM knob domain solution in HBS-EP buffer (GE healthcare), using a flow rate of 30 μL/min and contact time of 300 seconds ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
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bioRxiv - Biochemistry 2022Quote: ... The SAFB2 SAP and RRM domains were further purified via size exclusion on a HiLoad 16/600 SD 75 (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... and the phosphorylated kinase domain of FGFR1 was collected after size exclusion chromatography purification using Superdex 75 10/300 GL column (GE Healthcare). To prepare the dephosphorylated form ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated at 4°C overnight with primary antibody (1:1000) and incubated for 1 hour at RT with secondary antibodies in either 2% PBA (GE healthcare, RPN418), 5% ELK or OBB (LI-COR ...
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bioRxiv - Molecular Biology 2021Quote: ... ActiCHO Feed A and ActiCHO Feed B (both GE Healthcare) started on day four based on a glucose-controlled feeding regimen ...
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bioRxiv - Biochemistry 2023Quote: ... whilst antigen-B was purified with rProteinA Sepharose (GE Healthcare). Affinity tags were cleaved by incubation with either thrombin (Merck ...
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bioRxiv - Plant Biology 2022Quote: ... The primary antibody was diluted 1:10,000 in TBST-Buffer with 2% Blocking (GE Healthcare) and incubated on the membrane for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Biochemistry 2019Quote: The RRM domain of RBFOX2 (amino acids 100-194) was cloned into the pGEX6P-1 expression vector (GE Healthcare, 28-9546-48) downstream of a GST-SBP tandem affinity tag ...
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bioRxiv - Biophysics 2019Quote: GST-fusion proteins (UBAN domains of OPTN, ABIN, NEMO and Ub) were cloned into pGEX-4T-1 (GE Life Sciences, Freiburg, Germany) and site-directed mutagenesis was performed by PCR to introduce desired amino acid substitutions.
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bioRxiv - Cell Biology 2021Quote: ... CALM ANTH domain and Ub proteins were further purified on a HiLoad 16/60 Superdex 200 column (GE Healthcare Biosciences, Pittsburg, PA) equilibrated with 20mM HEPES-NaOH ...
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Intradermal Administration of Rescue Dose of Antiemetics in Chemotherapy-induced Nausea and VomitingbioRxiv - Pharmacology and Toxicology 2023Quote: ... and an ultrasound gel (LOGIQLEAN bottle hard type, GE Healthcare Japan) (30) ...