Labshake search
Citations for GE Life Sciences :
51 - 100 of 204 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Mononuclear cells (MNC) were extracted from fresh BM using Ficoll-Paque Plus (GE Healthcare, #17-1440-02). Bone marrow MNCs were also extracted from two patients (ALL10 and ALL12 ...
-
bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
-
bioRxiv - Immunology 2020Quote: ... The resuspended cells were subjected to lymphocyte enrichment using Ficoll-Paque Plus (17-1440-02, GE Healthcare). The decidual leukocytes were further purified by fluorescent activated cell sorting with 7-AAD (420404 ...
-
bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and mixed with fresh 80% Percoll (GE Healthcare, Cat. no. 17-5445-02) prepared in mitochondrial dilution buffer (1000 mM sucrose ...
-
bioRxiv - Molecular Biology 2022Quote: ... Clarified supernatants were then incubated with 150 mL slurry of IgG beads (GE Healthcare, 17-0969-02) for 2h at 4 °C on a turning wheel ...
-
bioRxiv - Neuroscience 2022Quote: ... myelin was removed by resuspending pelleted cells into 30% isotonic Percoll (GE Healthcare, Cat#17-5445-02) and centrifuging at 300g for 15 minutes at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... the cortex was homogenized and resuspended with 5 ml 70% Percoll Plus (GE Healthcare, 17-5445-02) and 5 ml 37% Percoll Plus placed on top ...
-
bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Reverse phase FPLC on a Resource RPC column (GE Healthcare, Danderyd, Sweden) in acetonitrile gradient in 0.1% trifluoroacetic acid was used for final purification of the mature peptides ...
-
bioRxiv - Immunology 2022Quote: ... Whole blood (20 mL) was layered upon a 15 mL Ficoll-Paque cushion (GE Healthcare, 17-1440-02) in 50 mL conical tubes ...
-
bioRxiv - Immunology 2020Quote: ... The resulting supernatant was discarded and the pellet resuspended in 3mL 70% Percoll (GE Healthcare, 17-0891-02). The Percoll gradient and density layering were prepared (from bottom to top ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was then incubated with 500 μl Glutathione Sepharose resins (17-5279-02, GE Healthcare Bio-Science) for 90 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... Mononuclear cells were extracted from bone marrow samples using Ficoll-Paque™ PLUS (GE Healthcare #17-1440-02), followed by red blood cell lysis using a red blood lysis buffer (Sigma #R7757).
-
bioRxiv - Biochemistry 2023Quote: ... Ammonium sulfate was eliminated by a desalting column step (HiPrep 26/10 desalting, GE Healthcare; ÄKTA Avant). The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg ...
-
bioRxiv - Immunology 2020Quote: ... Which Peripheral blood after dilution were subjected to lymphocyte enrichment using Ficoll-Paque Plus (17-1440-02, GE Healthcare). The peripheral blood leukocytes were further purified by fluorescent activated cell sorting with 7-AAD (420404 ...
-
bioRxiv - Biochemistry 2023Quote: ... A Multiphor II (GE Healthcare) was used for isoelectric focusing ...
-
bioRxiv - Biochemistry 2023Quote: ... Ammonium sulfate was removed by injection on a desalting column (HiPrep 26/10 desalting, GE Healthcare; ÄKTA Avant). The sample was concentrated before separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg ...
-
bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated hMDH preps were loaded onto a 5 mL HiTrap Butyl HP column (GE Healthcare). An inverse linear gradient of ammonium sulfate from 1.5 M to 0 M was used to purify hMDH where it eluted at a conductivity of 135 mS/cm ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was applied to a 5-ml His-Trap HF column charged with NiCl2 (GE Healthcare, 17-5248-02). GFP-NONO_WT or GFP-NONO_ΔRRM1 was eluted at room temperature with an imidazole gradient (25-500 mM ...
-
bioRxiv - Immunology 2021Quote: ... PBMCs were isolated from EDTA blood using density gradient separation in Ficoll Paque Plus reagent (GE Healthcare, #17-1440-02). After separation ...
-
bioRxiv - Biophysics 2022Quote: ... After loading the cell lysate onto a Protein L beads (Capto™ L, GE Healthcare, Cat. no. 17-5478-02) column ...
-
bioRxiv - Cancer Biology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were separated by density gradient centrifugation using Ficoll-Paque Premium (#17-5442-02; GE Healthcare). PBMCs were washed 3x with PBS ...
-
bioRxiv - Genetics 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated in two hours by using Ficoll Paque Plus (17-1440-02, GE Healthcare), deposited in RNAlater (Am7021#RNAlater Soln ...
-
bioRxiv - Plant Biology 2022Quote: ... The supernatant was filtrated through 0.45 µm filter and loaded into HisTrap HP column (GE Healthcare, Cat#:17-5248-02). The collected fractions were concentrated by Amicon® Ultra-15 Centrifugal Filter Units (EMD Millipore ...
-
bioRxiv - Biochemistry 2023Quote: ... Batch binding was performed overnight with Glutathione resin (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat. no. 17-5132-02) at 4°C ...
-
bioRxiv - Biophysics 2020Quote: ... the protein was separated on a RESOURCE™ reversed phase chromatography column (GE Healthcare) using a 0-70 % acetonitrile gradient ...
-
bioRxiv - Microbiology 2024Quote: ... All phase-contrast andfluorescence microscopy were conducted on the V3 DeltaVision Elite (GE Healthcare) with filter sets for GFP/FITC (excitation=464-492 nm ...
-
bioRxiv - Plant Biology 2020Quote: ... Excess iron ammonium citrate and lithium sulfide was removed by desalting using a NAP™ 25 column (GE Healthcare). Subsequently ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... RNA was then precipitated in ethanol/ammonium acetate and purified by G25 size exclusion chromatography (illustraTMNAPTM-10, GE Healthcare). RNA purity was assayed by denaturing gel electrophoresis.
-
bioRxiv - Microbiology 2022Quote: ... the purified paLptDE or sfLptDE protein samples were buffer exchanged into 150 mM ammonium acetate (pH 8.0) containing 0.5% C8E4 (v/v) on a Superdex 200 gel filtration column (GE Healthcare). LptDE samples were subsequently introduced via gold-coated nanospray capillaries prepared in house and MS spectra were recorded on a Synapt G2-Si instrument (Waters ...
-
bioRxiv - Systems Biology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of healthy donors by Ficoll-Paque (GE Healthcare, 17-1440-02) density gradient centrifugation ...
-
bioRxiv - Cancer Biology 2021Quote: Approximately 500,000 cells were transfected using a final concentration of 20 nM EPSTI1 (J-015094-09-0020 and J-015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 µL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
-
bioRxiv - Biophysics 2022Quote: ... Clarified lysate was passed through a 10 ml Ni2+-Sepharose 6 Fast flow resin (GE Healthcare Catalogue number 17-5318-02), washed with 50 ml of binding buffer (50mM Tris ...
-
bioRxiv - Cell Biology 2022Quote: ... Cat# 015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 μL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
-
bioRxiv - Biophysics 2022Quote: ... the eluted protein buffer was exchanged with phosphate buffered saline (PBS, Biological Industries, Reference number: 02-023-1A) using a desalting column (Sephadex G25, GE Healthcare). Protein labeling levels were determined by absorption at 450 nm ...
-
bioRxiv - Microbiology 2023Quote: ... This suspension was slowly added to the top of 4 mLFicoll-Paque density gradient (GE Healthcare #17-1440-02, Uppsala, Sweden) and centrifuged for 40 minutes at 400 x g ...
-
bioRxiv - Neuroscience 2023Quote: ... and pellets were resuspended in 6 mL 25 % Standard Isotonic Percoll (Percoll: GE Healthcare, 17-5445-02 with 10% 10x PBS) in Medium A ...
-
bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation using a Ficoll-Paque Plus gradient (GE Healthcare, Cat #17-1440-02), then washed by centrifugation ...
-
bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood using density centrifugation with Ficoll-Paque PREMIUM density gradient (GE Healthcare, Cat# 17-5442-02) and SepMate™ tubes (Stemcell ...
-
bioRxiv - Molecular Biology 2024Quote: Raw 3-phase images generated by 3D-SIM were reconstructed using SoftWorX (Applied Precision, GE), and these reconstructed stacks were 3D rendered using the 3D script plugin 31 in FIJI ...
-
bioRxiv - Immunology 2020Quote: ... The supernatant was harvested 5 days post transfection and IgG was affinity purified using Protein A Sepharose (GE Healthcare, #17-5280-02). SDS-PAGE was used to confirm the purity of IgG and adequate cleavage of the P2A peptide ...
-
bioRxiv - Cell Biology 2022Quote: ... We performed immunoprecipitations with precoated anti-SRSF9 or rabbit normal IgG-protein A Sepharose beads (GE Healthcare, catalog number: 17-1279-02) for 2 □ hours at 4□°C ...
-
bioRxiv - Neuroscience 2021Quote: ... human peripheral blood mononuclear cells were isolated from buffy coats obtained from healthy volunteers (Niguarda Hospital) after Ficoll®Paque Plus (Cat. 17-1440-02, GE Healthcare) density gradient centrifugation ...
-
bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated by density gradient centrifugation using the Ficoll® Paque Plus solution (17-1440-02, GE Healthcare).
-
bioRxiv - Microbiology 2023Quote: Blood was drawn from five healthy donors and PBMCs were isolated using Ficoll-Paque Plus (17-1440-02, GE Healthcare, Danderyd, Sweden) gradient centrifugation ...
-
bioRxiv - Plant Biology 2024Quote: ... The cleared lysate was supplemented with 20 mM imidazole and loaded on a HisTrap HP column (GE Healthcare, Cat#: 17-5248-02). The peak fractions containing the recombinant proteins were pooled and digested by adding 30 μg of His-SUMO Protease during dialysis with 2 L buffer (20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biophysics 2020Quote: ... centrifuged at 14000g and 4 °C for 5 min and the excess of ammonium sulfate was removed by running the supernatant through a NAP-25 column (GE Healthcare). The tAv was purified using HiTrap chelating column (GE Healthcare ...
-
bioRxiv - Microbiology 2022Quote: ... Erythrocyte lysis was performed using ammonium-chloride-potassium (ACK) buffer and leukocytes were isolated by gradient centrifugation using Percoll solution (GE Healthcare). Lung tissue leukocytes were filtered again and antibody staining for flow cytometry was performed.
-
bioRxiv - Cell Biology 2022Quote: ... Myristoylated Arf1 was enriched by ammonium sulfate precipitation (35% saturation) then applied to HiLoad 16/60 Superdex 75 column (GE Healthcare) in 20 mM Tris at pH 8.0 ...