Labshake search
Citations for GE Life Sciences :
51 - 100 of 3076 citations for 6 Chloro 5 methoxypyridin 2 amine hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... The mixture was analyzed by size exclusion chromatography using Superose 6 Increase 5/150 GL column (GE Healthcare) mounted on an AKTApure system ...
-
bioRxiv - Biochemistry 2019Quote: Analytical size-exclusion chromatography (analytical SEC) was performed on a Superose 6 5/150 GL column (GE Healthcare) connected to an ÄKTAmicro FLPC system (GE Healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: ... was immobilised on a CM5 chip through amine conjugation using NHS/EDC coupling using a Biacore X100 (GE Healthcare). Samples were run at 30 μL/min with an injection time of 60 s and a dissociation of 200 s ...
-
bioRxiv - Microbiology 2020Quote: ... pH 4) was covalently immobilized on a CM5 sensor chip using an Amine coupling kit (GE Healthcare Life Sciences). Binding analyses were performed in HBS-P buffer (0.01 M HEPES ...
-
bioRxiv - Neuroscience 2021Quote: ... hTau and mTau were immobilised onto an amine-coupling activated (NHS/EDC) CM5 Series S Sensor Chip (GE Healthcare) with a contact time of 180 s at 5 µL/min flow rate and at 25°C ...
-
bioRxiv - Immunology 2023Quote: ... Binding responses were measured following immobilization by amine coupling of antigens (37) on CM5 sensor chips (BIAcore/GE Healthcare). Purified plasma IgG samples at 200 µg/ml were flowed (2.5 min ...
-
bioRxiv - Neuroscience 2021Quote: ... The chip was deactivated by 1 M Ethanolamine hydrochloride-NaOH (GE Healthcare Life Sciences) at a flow rate of 10 μL/min for 420 s ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 µM Drp1 was injected onto a Superose 6 10/300GL column in an ÄKTApure FPLC system (GE Healthcare) connected in line with DAWN Heleos-II 18-angle MALS and Optilab T-rEX differential refractive index (dRI ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) equilibrated in Dynein-lysis buffer and incubated for 3-4 h with rotation along the long axis of the tube ...
-
bioRxiv - Cancer Biology 2021Quote: ... sfGFP-POX10 was diluted to 5 µg/mL in sodium acetate buffer (pH 4.5) and subsequently covalently immobilized on a CM5 chip by amine coupling according to the manufacturer’ ss protocol (GE Healthcare). Next ...
-
bioRxiv - Biophysics 2019Quote: ... chip was prepared by amine coupling of anti-mouse IgG antibodies at pH 5.0 (Mouse antibody capture kit, GE Healthcare) according to the protocol described by manufacturer ...
-
bioRxiv - Microbiology 2020Quote: ... Pure FasR and FasRF123A were dialysed against 10 mM sodium acetate pH 4.5 and coupled to a CM5 sensor chip using the Amine Coupling Kit (GE Healthcare). Micromolar concentrations of C20-CoA were dialysed against 25 mM Tris.HCl pH 8 ...
-
bioRxiv - Developmental Biology 2021Quote: ... pH 4.0 were immobilized on a Biacore CM5 sensor chip (research grade) with the amine coupling kit following the manufacturer’s instructions (GE Healthcare). 1700 resonance units of FBN2-Ct or ADAMTS6-Ct was coupled to the chip for analysis in a Biacore 3000 instrument (GE Healthcare) ...
-
bioRxiv - Microbiology 2022Quote: ... 2006 35 was covalently immobilized on a CM7 Series S sensor chip using an amine coupling reagent kit (GE Healthcare) at the range level of 3,000 to 5,000 response units (RU) ...
-
bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
-
bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
-
bioRxiv - Microbiology 2020Quote: ... Protein sample were injected into a size exclusion column (Superose™ 6 Increase 5/150 GL, GE Healthcare Bio-Sciences) previously equilibrated with corresponding buffer and eluted directly into the SAXS flow-through capillary cell at a flow rate of 0.2 ml/min ...
-
bioRxiv - Biophysics 2023Quote: ... the Ni-NTA eluate was applied to size exclusion chromatography on a Superose 6 Increase 5/150 column (GE Healthcare) in basic buffer supplemented with 0.003 % LMNG and 0.0003 % CHS.
-
bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
-
bioRxiv - Microbiology 2020Quote: ... The IgG anti-SARS-CoV-2 was purified from plasma of a convalescent patient using a 5/5 HiTrap rProtein-A column (GE Healthcare, USA). See detailed information about this procedure in Supplementary method No.1.
-
bioRxiv - Biochemistry 2019Quote: ... The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2 hr on a roller ...
-
bioRxiv - Plant Biology 2021Quote: ... 2 mM PMSF and 1x protease-inhibitor cocktail) and loaded onto a Superose™ 6 Increase column (GE Healthcare). Twenty-four fractions of 0.5 mL were collected and precipitated as described (Esteve-Bruna et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2 hr on a roller ...
-
bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) and incubated for 2-3 h with rotation along the long axis of the tube ...
-
bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 2 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... The clarified supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) and incubated for 2-3 h with rotation along the long axis of the tube ...
-
bioRxiv - Biochemistry 2022Quote: ... 10 ml of media was concentrated over 2 ml heparin affinity medium (Heparin Sepharose 6 Fast Flow, GE Healthcare) packed into a 10 ml Poly-Prep Chromatography gravity flow column (Bio-Rad) ...
-
bioRxiv - Bioengineering 2022Quote: ... the receptor fragment MET567 comprising the N-terminal SEMA domain and the cysteine-rich/PSI domain was immobilized at low-density (515 RU) using amine-coupling on a CM5 sensor chip (GE Healthcare). A blank inactivated reference channel was used to correct for non-specific binding ...
-
bioRxiv - Immunology 2021Quote: ... PPACK-thrombin or fibrinogen in acetate buffer pH4.5 was immobilized to a chip surface (1000 or 10000 RU) using NHS/EDC-amine coupling and residual binding sites blocked by 1M ethanolamine (GE Healthcare). FH (0.5-62nM ...
-
bioRxiv - Microbiology 2020Quote: ... Mouse anti-human Fc monoclonal antibody R10Z8E9 was immobilised onto active and reference flow cells of a Series S sensor CM5 chip using an amine capture kit (both from GE Healthcare). Approximately 1000 response units of Pf34 bait protein was captured onto the active flow cell ...
-
bioRxiv - Cell Biology 2019Quote: ... as capture molecule to the dextran surface of both flow cells of a CM5 Chip (GE Heathcare) using an amine coupling kit (GE healthcare). GST tagged ligand proteins were captured on the detection flow cell of the chip and free GST was captured on the reference flow cell ...
-
bioRxiv - Immunology 2023Quote: ... An Fc capture chip was produced by amine coupling of anti-mouse IgG antibody to a CM5 chip using a commercial Fc Capture Kit (GE Healthcare). First ...
-
bioRxiv - Biophysics 2023Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
-
bioRxiv - Plant Biology 2023Quote: ... 5-mM 2-mercaptoethanol) before being applied to a HiTrap Heparin HP (GE Healthcare), and eluted by a linear NaCl gradient up to 1.0 M ...
-
bioRxiv - Molecular Biology 2019Quote: ... The protein was then concentrated and subjected to size-exclusion chromatography on a Superose 6 5/150 column (GE Healthcare, Germany) in plunging buffer (20 mM TRIS-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: ... concentrated in a vivaspin 100,000 MWCO and loaded onto a Superose 6 5/150 size exclusion chromatography column (GE Healthcare Lifescience) to remove thrombin and exchange the buffer to either 20 mm ...
-
bioRxiv - Biochemistry 2022Quote: ... The separated species were concentrated to 1.3 - 2.5 mg/ml and 200 µl of each were applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with SEC buffer and connected to a miniDAWN TREOS II triple-angle light scattering detector (Wyatt Technologies ...
-
bioRxiv - Immunology 2022Quote: ... The cell suspension was centrifuged at 1,200 rpm at 4°C for 6 minutes and the pellet was resuspended in 5 ml of 40% Percoll (GE Healthcare) in PBS and transferred to a 15 ml tube ...
-
bioRxiv - Biochemistry 2022Quote: ... were solubilised in TK buffer supplemented with 6 M urea before loading onto a 5 ml HisTrap HP column (GE Healthcare). The column was first washed with TK + 6 M urea followed by TK + 6 M Urea + 50 mM imidazole ...
-
bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...
-
bioRxiv - Biochemistry 2023Quote: ... the sample was treated with 100 mM phosphate buffer pH 7.5 and 50 mM EDTA before purification by gel filtration at room temperature on a Superose-6 Increase column (GE Healthcare) equilibrated with buffer containing 25 mM TRIS pH 8 ...
-
bioRxiv - Biochemistry 2023Quote: ... A 2-fold molar excess of LF was mixed with 925 pmoles of hexon and incubated on ice for 5 min followed by SEC on Superose 6 increase 10/300 (GE Healthcare) equilibrated with buffer D ...
-
bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to ∼2 µM for cryo-EM sample preparation.
-
bioRxiv - Neuroscience 2024Quote: Molecular masses of purified gephyrin and variants were detected by analytical size exclusion chromatography (SEC) using a Superose 6 increase 5/10 (GE Healthcare). Typically ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The urea concentration was set to 6 M and the sample was applied to a 5 mL HiTrap QFF anion exchange column (GE healthcare) and was eluted using a 30 CV long gradient from low salt buffer (20 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20 mM HEPES ...
-
bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 2µM for cryo-EM sample preparation.
-
bioRxiv - Neuroscience 2019Quote: ... Each probe contains a 3’-end amine-modified nucleotide that allows directly couple to an NHS-ester Cy3 dye (GE Healthcare, PA23001) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...
-
bioRxiv - Biochemistry 2019Quote: ... The sample was incubated 2 h on ice then applied onto a Superose 6 Increase 3.2/300 gel filtration column (GE Healthcare) equilibrated in 20 mM Tris-acetate (pH 7.5) ...