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Citations for GE Life Sciences :
901 - 942 of 942 citations for Recombinant Mouse Lgals1 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... followed by horseradish peroxidase (HRP)-labeled secondary antibody goat-anti-mouse IgG Fc (Product #: 31433) (Pierce Antibodies) or donkey-anti-rabbit IgG (Product #: NA934V) (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Physiology 2019Quote: ... 20 islets per well (n = 3 biological replicates per group) were transfected with control (non-targeting #2) siRNA or mouse Brd4 SMARTpool siRNA (Dharmacon GE Life Sciences) at 100 nM each with lipofectamine 3000 according to the product instructions ...
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bioRxiv - Immunology 2019Quote: ... followed by horseradish peroxidase (HRP)-labelled secondary antibody goat-anti mouse IgG Fc (Pierce Antibodies) or donkey-anti-rabbit IgG (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Microbiology 2020Quote: ... Plates were washed three times with PBS (0.05% Tween20) and incubated with sheep-derived anti-mouse total IgG (GE Healthcare, Amersham, UK), IgG1 or IgG2a (Invitrogen ...
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bioRxiv - Synthetic Biology 2022Quote: ... they were soaked in TBS-t for 30 min at room temperature with HRP-conjugated anti-mouse (GE Healthcare, Chicago, IL, USA) or anti-rabbit antibody (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... the following HRP conjugated secondary antibodies were used: for Cas9 - anti-mouse (1:10,000 in 1% milk PBS-T, GE Healthcare, code NA931V); for γ-H2A - anti-rabbit (1:2000 in 1% milk/PBS-T ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times in PBS-T before it was incubated with the secondary antibody goat α-mouse IgG (H&L)-HRP (1:10’000, GE healthcare #NXA931). After 1 h incubation ...
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bioRxiv - Biochemistry 2023Quote: ... for 5 min each time) and incubated with Bullet ImmunoReaction Buffer (Nacalai Tesque, 18439-85) containing peroxidase-conjugated anti-mouse IgG (GE Healthcare, NA931) or anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies were horseradish peroxidase (HPR)-conjugated (Sigma-Aldrich A0545, anti-rabbit 1:10000; GE Healthcare NA931V, anti-mouse 1:5000). Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH ...
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bioRxiv - Cell Biology 2019Quote: ... Mixed asexual blood stage parasites collected from an infected mouse by cardiac puncture were filtered through CF11 cellulose columns (Whatman-GE Healthcare Life Sciences) to exclude mice white blood cells and immediately lysed with ice-cold erythrocyte lysis buffer (1.5M NH4Cl ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were washed with 1x TTBS and placed in a 1:25000 secondary HRP-conjugated IgG anti-rabbit or anti-mouse solution (GE Healthcare®, Sweden) according to the primary antibody datasheet specifications ...
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bioRxiv - Cancer Biology 2019Quote: ... Chemiluminescence detection was performed after incubation with the secondary antibody ECL (Anti-Mouse IgG Horsradish Peroxidase linked whole antibody: GE Healthcare UK limited) for 2h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunocomplexes were detected using sheep anti-mouse or donkey anti-rabbit immunoglobulin secondary antibodies conjugated to horseradish peroxidase (HRP) (GE Healthcare Europe GmbH) and visualized by enhanced chemiluminescence (Super Signal West Pico ...
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bioRxiv - Biophysics 2022Quote: The rat Syb2 (29–93) and Syb2 (29–93, S61C), mouse Rab3A (22–217, Q81L) were all cloned into pGEX-6p-1(GE Healthcare; Piscataway, NJ). The cytoplasmic domain of rat Syx1 (2–253 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with TBS + 0.1% Tween and incubated with goat anti-rabbit (1:2000, #NA934V) or anti-mouse (1:2000, #NA931V) secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Illinois) at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2019Quote: ... characterization of an N-terminal Aβ peptide DAE-EG (DAEFRHDSGYSGKQKSRNEGKGGC) binding to anti-mouse captured antibodies from hybridoma supernatants was performed using a BIAcore T-200 instrument (GE Healthcare, Marlborough, MA) [36] ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were washed for 3x 5 minutes with TBST and incubated for 1 hour at RT with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG, Jackson ImmunoResearch 111-035-144 or sheep anti-mouse IgG, GE Healthcare NA931V) diluted 1:5000 in 5% milk / TBST ...
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bioRxiv - Epidemiology 2021Quote: ... CM5 chips for surface plasmon resonance (SPR) assay and Cytometric Bead Array (CBA) Mouse Immunoglobulin Isotyping Kit were purchased from GE HealthCare (Boston, USA). Ninety-six-well MultiScreen HTS plates were purchased from Millipore (Billerica ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated in PBST containing a 1:2500 diluted secondary antibody (horseradish peroxidase-linked anti-mouse IgG from sheep; GE Healthcare, Cat. NA931V). After 3 rounds of 5-min wash with PBST ...
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bioRxiv - Microbiology 2023Quote: ... probed with a 1:2,000 dilution of polyclonal α-PotA (Lin et al., 2017) followed by a 1:10000 dilution of peroxidase labeled α-mouse (GE Healthcare) and developed with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... the membranes were incubated with secondary antibody coupled with Horseradish Peroxidase (anti-rabbit, anti-rat or anti-mouse, Amersham, GE Healthcare, 1/3000) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed in 1 x TBST and incubated in the appropriate secondary antibodies (anti-Rabbit, GE Healthcare, NA934V; anti-mouse, GE Healthcare, NA931V) for 2 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Peroxidase-linked anti-mouse immunoglobulin G and peroxidase-linked antirabbit immunoglobulin G (both Cytiva, formerly known as GE Healthcare Bio-Sciences NA931 and NA934), Alexa Fluor 594-conjugated Donkey Anti-Mouse IgG (1:250 ...
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bioRxiv - Neuroscience 2020Quote: Fusion proteins of glutathione-S-transferase and mouse Clstn3 (JK091; aa 21–244) were produced in BL21 Escherichia coli and purified using a glutathione-Sepharose column (GE Healthcare, Chicago, IL, USA). Clstn1 peptides (JK025 ...
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bioRxiv - Immunology 2020Quote: Approximately 200 μL of normal mouse serum or anti-CD200 serum day 157 (after 4X immunizations) were purified using a protein G SpinTrap (GE Healthcare # 28-4083-47) according to the manufacturer’s instrutions ...
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bioRxiv - Physiology 2021Quote: ... Femur and tibia were removed and the marrow cavity was flushed out with 10mL 1x phosphate-buffered saline per mouse (GE Healthcare Life Sciences, MA) with a 25 G needle (Becton ...
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bioRxiv - Cell Biology 2022Quote: ... and membranes were incubated with secondary antibodies conjugated to horseradish peroxidase (anti-mouse or anti-rabbit; GE Healthcare 17097199 and 16951542, 1:5000 dilution) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 5 times with PBS-T and incubated with a 1:5000 dilution of anti-mouse IgG-HRP (GE healthcare cat. # NA931-1ml), or with a 1:100000 dilution of anti-ferret IgG-HRP (Abcam cat ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 1 h at room temperature with 1:10,000 dilutions of anti-rabbit or anti-mouse secondary antibodies (GE Healthcare Life Sciences, Pittsburgh, PA), washed 3X with TBS-T ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed with PBST and incubated with alkaline phosphatase-conjugated secondary antibodies (anti-rabbit IgG and anti-mouse IgG, GE healthcare life sciences, 1:10000) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated for 90 minutes at room temperature with secondary antibodies (donkey anti-rabbit HRP-IgG, GE Healthacare, cat#NA934V, 1:5000 or goat anti-mouse HRP-IgG, GE Healthcare, cat#NA931V, 1:2000) diluted in blocking solution and washed as before ...
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bioRxiv - Biophysics 2019Quote: ... The membrane was rinsed three more times in TBS-Tw and incubated for 30 min with secondary antibody (HRP-conjugated sheep anti-mouse IgG, GE Healthcare Life Sciences; Freiburg, Germany) diluted 1:20,000 in TBS-Tw + 1% milk ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution in 3% milk overnight at 4°C before incubation with secondary HRP-linked sheep anti mouse (NA931-1ML, GE Healthcare Life Sciences, Buckinghamshire, UK) at a 1:3000 dilution in 5% milk for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by an incubation with horseradish peroxidase (HRP) secondary antibodies (Mouse IgG HRP Linked Whole Ab-GE Healthcare, #NA931, 1:5000; Rabbit IgG HRP Linked Whole Ab-GE Healthcare, #NA934, 1:5000) in 3% milk in TBST ...
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bioRxiv - Microbiology 2023Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare, Chicago, IL, United States). The signal was visualized in a Chemidoc MP imaging system (Bio Rad)s).
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used were peroxidase-linked anti-mouse IgG and peroxidase-linked anti-rabbit IgG (both Cytiva, formerly known as GE Healthcare Bio-Sciences, NA931 and NA934; 1:5000), Alexa Fluor 594-conjugated donkey anti-mouse IgG (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA; Goat AntiRat – GE Healthcare Life Sciences, Little Chalfont, UK) for 1 hr at 24 °C with agitation ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA; Goat Anti-Rat – GE Healthcare Life Sciences, Little Chalfont, UK) for 1hr at 24°C with agitation ...