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Citations for GE Life Sciences :
901 - 950 of 1831 citations for Calponin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysates were loaded onto Ni-Sepharose beads and nutated for ∼1 h (GE Healthcare). Beads were washed twice with denaturing buffer and hexa-histidine-tagged proteins were eluted with the same buffer supplemented with 500 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... The plates were washed with PBST and incubated with anti-M13-HRP 1:3,000 (GE Healthcare) in blocking buffer for 30 min ...
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bioRxiv - Bioengineering 2020Quote: ... The collected supernatant was purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... the respective supernatants were applied to 1/5 ml HiTrap™ Q XL columns (GE Healthcare) for anion exchange chromatography using the ÄKTA Start chromatography system (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg of cell lysate was incubated with 40 μl of glutathione-Sepharose beads (GE Healthcare) at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... sheep anti-mouse horseradish peroxidase-linked antibody (1:3000, NA931V, GE Healthcare Life Sciences, Uppsala, Sweden), donkey anti-rabbit horseradish peroxidase-linked antibody (1:4000 ...
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bioRxiv - Molecular Biology 2019Quote: ... donkey anti-rabbit horseradish peroxidase-linked antibody (1:4000, NA934V, GE Healthcare Life Sciences, Uppsala, Sweden).
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bioRxiv - Biochemistry 2019Quote: ... pH 7.0) and loaded to a 1-ml HiTrap Protein G Sepharose FF column (GE Healthcare). The column was washed with binding buffer until the UV280 absorbance dropped to a stable baseline ...
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bioRxiv - Zoology 2021Quote: ... One of the obtained PCR products was purified with ExoProStar 1-Step kit (GE Healthcare US77702) and Sanger-sequenced directly at Macrogen (Netherlands) ...
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bioRxiv - Biochemistry 2020Quote: ... and then incubated with horseradish peroxidase-linked anti-mouse IgG (1:1000) (GE Healthcare Life Sciences). After washing ...
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bioRxiv - Cancer Biology 2021Quote: ... and after 1 and 2 days of co-culture on an INCell Analyzer 2200 (GE Healthcare), with a large field-of-view sCMOS camera (resolution 2048×2048 pixels ...
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bioRxiv - Molecular Biology 2021Quote: ... TOFU-6/PICS-1 was further purified by HiLoad 16/600 Superdex 75 column (GE healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... pH 5.0 immediately before purification on a HiTrap Protein G HP 1 mL column (GE Healthcare) using an AKTA pure instrument ...
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Adaptation of the Romanomermis culicivorax CCA-adding enzyme to miniaturized armless tRNA substratesbioRxiv - Molecular Biology 2020Quote: ... sterile filtrated and loaded onto a HisTrap FF 1 ml or 5 ml column (GE Healthcare). Column wash was performed with 4-10 column volumes of binding buffer (25 mM Tris/HCl pH 7.6 ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM β-mercaptoethanol) and applied to a 1-ml Hi-Trap Heparin column (GE Healthcare) equilibrated with TGED ...
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bioRxiv - Microbiology 2019Quote: ... lysed and the cell lysate was loaded onto a 1 ml GSTrap HP column (GE Healthcare) before elution using 50 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2019Quote: ... and then incubated for two hours in secondary antibody – anti-rabbit-HRP (1:5000, GE Healthcare) or anti-mouse-HRP (1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... The mixture was loaded onto a Buffer-A-equilibrated 1 mL HiTrap QFF column (GE Healthcare). Then the column was washed by 10 column volumes of Buffer A ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 1 h at room temperature in the appropriate HRP-labelled secondary antibody (GE Healthcare). Blots were developed with ECL Clarity (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... Biotin incorporation was assessed by incubating blots with streptavidin-HRP (1:1000 v/v, GE Healthcare), and vimentin levels estimated by western blot with anti-vimentin V9 monoclonal antibody (1:1000 v/v ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT using a HiPrep 26/10 desalting column (GE Healthcare, Cat# 17-5087-01). DrrA-OCRL or DrrA-INPP5E were then loaded onto a 1 mL MonoS (5/50 GL ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT) and bound protein was eluted directly onto a HiTrap Heparin column (GE Healthcare) using buffer Hep A supplemented with 2.5 mM Desthiobiotin ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were further purified using a 1 or 5 ml Hi-trap Q column (GE Healthcare) that was equilibrated in (100 mM NaCl,10 mM KH2PO4 ...
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bioRxiv - Microbiology 2021Quote: ... The filtered cell lysate was loaded onto a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: The two animals (Monkey 1 and Monkey 2) were scanned on a Discovery MI (GE Healthcare) PET/CT scanner ...
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bioRxiv - Immunology 2020Quote: ... digested products were applied to a 1-mL HiTrap MabSelect SuRe column (GE Healthcare Life Sciences) and the flow-through containing cleaved Fabs was collected ...
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bioRxiv - Immunology 2021Quote: ... Supernatant from transfected CHO cells was purified using 1 ml HiTrap MabSelect PrismA (GE Healthcare – 17549851) affinity chromatography with in-line dialysis in PBS via HiTrap 5 ml desalting columns (GE Healthcare – 29048684 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM TCEP and loaded to 5 ml HiTrap SP HP (GE Healthcare, 17-1152-01). After extensive washing ...
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bioRxiv - Microbiology 2022Quote: ... Filtered lysates with strep-tagged proteins were applied onto StrepTrap HP 1 ml columns (GE Healthcare). Subsequent protein separation was conducted using the ÄKTA start chromatography system (GE ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred (100 V, 1 hr) onto PVDF (polyvinylidene difluoride) membranes (GE Healthcare Life Sciences) using 1X Tris/Glycine buffer (in-house 50X solution containing 250 mM Tris and 1920 mM Glycine) ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting clarified lysate was batch-bound to ~1 mL of settled IgG sepharose (GE Healthcare) equilibrated in lysis buffer and allowed to incubate on a roller for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Further purification method was carried out with a 1 mL HisTrap HP column (GE Healthcare, Sweden) to obtain the pure protein ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 2h at RT with streptavidin-HRP (1:400, GE Healthcare UK). After washing ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were loaded on a 5 ml HisTrap™HP column (1 mL/min; GE Healthcare) in a binding buffer containing 100 mM tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: Lysates were purified using a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) connected to an ÄKTA pure protein purification system (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: ... and filtered onto 1 μm polycarbonate membrane filters (142 mm diameter, Whatman/GE Healthcare, Uppsala, Sweden) representing the viral fraction ...
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bioRxiv - Immunology 2022Quote: ... Th gB-1 mRNA was purified by fast protein liquid chromatography (FPLC; Akta purifier; GE Healthcare)79 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody was detected with HRP-linked donkey anti–rabbit IgG (GE Healthcare, #NA934V; 1:10,000) or HRP-liked sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
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bioRxiv - Biochemistry 2023Quote: ... the solubilized enzymes were loaded onto a 1 mL Q-sepharose HP column (GE Healthcare, UK) pre-equilibrated in buffer containing 20[mM Tris-HCl (pH 7.14 at 20[°C) ...
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bioRxiv - Biochemistry 2022Quote: ... and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000; GE Healthcare; NA943V). Membrane was visualized using Radiance Plus chemiluminescent HRP substrate (Azure Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000; GE Healthcare; NA943V). Membrane was visualized as described above.
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bioRxiv - Cell Biology 2022Quote: ... and cloned into BamHI and NotI restriction sites within the pGEX-4T-1 plasmid (GE Healthcare), such that the gene was in frame with an N-terminal GST tag (pGEX-4T-1-GST-KIN-EC-ter) ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies: 1:10000 goat α rabbit or goat α mouse ECL (GE healthcare NA931, NA934).
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used: sheep anti-mouse (HRP-conjugated, GE Healthcare, NXA931V, 1:10000), donkey anti-rabbit (HRP-conjugated ...
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bioRxiv - Synthetic Biology 2023Quote: ... Recombinant proteins were then purified by 1 mL Ni Sepharose (17-5318-01, GE Healthcare, USA). Columns were sequentially washed with 20 mL buffer 1 and 10 mL buffer 2 (in mM ...
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bioRxiv - Biochemistry 2023Quote: ... The diluted sample was then applied to a 1 mL HiTrap Q HP column (GE Healthcare), and UBXD1 was eluted with a 0-50% gradient of AEX elution buffer (AEX binding buffer supplemented with 1000 mM KCl) ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtered (Filter paper, Grade 1; Size: 185 mm; Whatman, GE Healthcare Live Sciences), a 1 mL aliquot of the filtrate was transferred into a 1.5 mL centrifuge tube ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified cpxA31-163 was cloned into BamHI/EcoRI-digested pGEX-6P-1 expression vector (GE Healthcare), which encodes for an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded on a 1 ml HisTrap HP Q column (GE Healthcare, Pittsburgh, PA), and the flow-through was collected and dialyzed against 20 mM HEPES pH 6.8 ...