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Citations for GE Life Sciences :
901 - 950 of 4094 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and Heparin HiTrap (GE Healthcare, 5 mL) chromatography ...
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bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Neuroscience 2022Quote: ... plasma or CSF probes were incubated in 25 μl for 1 hour at room temperature (diluted with PBS containing 5% Amersham ECL Blocking Agent, GE Healthcare RNP2125), followed by additional washing steps and incubation with an anti-mouse secondary antibody conjugated with the MSD Sulfo-tag for detection ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed 3×10mins with TBS-T and 1×10mins with TBS before developing the signal by incubating the membrane in Amersham Hyperfilm ECL for 5 minutes (GE Healthcare, UK).
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bioRxiv - Neuroscience 2022Quote: ... Images were captured one frame every 1 min 30 sec over 5 h using a DeltaVision Imaging System (Applied Precision, Bratislava, Slovakia).
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bioRxiv - Microbiology 2024Quote: Purified PmSLP-1 and FI were mixed to achieve a final concentration ratio of 5:1 and incubated on ice for 10mins before loading onto a Superdex Increase 200 10/300 GL column (GE Life Sciences). The column was pre-equilibrated in 1X PBS pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for 1 hour prior to purification on a Superdex 200 10/300 size-exclusion column (GE Healthcare). Rac1 (G12V ...
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bioRxiv - Biophysics 2021Quote: ... After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare) on an FPLC (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: 50 μl of the N protein samples (1 mg/ml) purified according to protocols 1 or 2 were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Peak fractions were diluted with 2 volumes of buffer H and loaded onto a 1-mL SP HP column (GE Healthcare) and washed with buffer C (50 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of freshly purified protein (concentration 1-2 mg/ml) was injected on a Superdex 75 300/10 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Immunology 2022Quote: ... mononuclear cells were isolated by density gradient centrifugation of PBS-diluted buffy coat/blood (1:2) over Ficoll-Paque Plus (GE Healthcare). The PBMC layer was carefully removed and washed 3 times with PBS.
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bioRxiv - Cancer Biology 2022Quote: ... Blood was diluted 1:2 with 1× phosphate-buffered saline (PBS) and transferred to a 50 ml falcon tube with Ficoll-Paque PLUS (GE Healthcare) at a 1:2 ratio ...
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bioRxiv - Cell Biology 2023Quote: ... were used at a dilution of 1:10,000 and the HRP-linked sheep F(ab’)2 fragment to mouse IgG (GE Healthcare, NA9310) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare, Chicago, USA).
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bioRxiv - Biochemistry 2022Quote: ... and passed through two subsequent gel filtration steps using a: 1) Hiload 16/60 Superdex 75 column (Cytiva #28989333) and 2) and Sephacryl S-300 column (GE Healthcare). Buffers used for this sizing step and subsequent labeling reactions were followed as for full-length proteins ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a dilution of 1:10,000 and the HRP-linked donkey F(ab’)2 fragment to rabbit IgG (GE Healthcare, NA9340) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... both extracts (Triton and SDS) were diluted 2:1 in 50 nM Tris-HCl and incubated with 50 µl Streptavidin Sepharose (GE Healthcare) overnight at 4°C while rotating ...
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bioRxiv - Molecular Biology 2024Quote: An appropriate amount (0.5 – 2 µg) of total RNA was separated in 1% MOPS-formaldehyde agarose gels and transferred to Hybond-N + membranes (GE Healthcare). Membranes were exposed to either randomly [32P]-labelled dsDNA probes ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM UltraGlutamine (GE Healthcare, UK), BME vitamins (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
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bioRxiv - Immunology 2020Quote: ... cDNA constructs were inserted into the pGEX6P-3 plasmid (GE Healthcare) and expressed as GST-fusion proteins in High efficiency BL21(DE3 ...
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bioRxiv - Cell Biology 2022Quote: Early passage HUVECs were coated on Cytodex-3 beads (GE Healthcare) at a density of 10 million cells/40 μl beads and incubated in suspension for 3-4 hours with gentle mixing every hour ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was then vacuum filtered and loaded at 1 mL/min onto a HiTrap Q HP column (5 mL, GE Healthcare Life Sciences) pre-equilibrated with buffer D [10 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: we incubated samples for 10 min with Buffer A (1 M GA, 80% ACN, 5% TFA) followed by incubation with the TiO2 Mag Sepharose (GE Healthcare Life Sciences) for 30 min in an Eppendorf Thermomixer at 800 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... 45 μl of the protein at 4.43 mg ml−1 (70.4 µM) was injected in SEC column S200i 5/150 (GE Life Sciences, Velizy-Villacoublay, France) with a buffer containing 20mM Hepes pH 7,5 ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted soluble fraction was then loaded at 1 mL/min in Buffer A onto a 5 mL HiTrap SPFF column (GE Healthcare Life Sciences) and eluted at 2 mL/min with a 70 mL gradient to 100% Buffer B ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by on-column digestion and the eluate was further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2022Quote: ... The protein solubilized in the previous step were subjected to a Ni HP-5 column (5 mL, GE Healthcare, USA), previously equilibrated with 5 CV of Buffer C (buffer C (50 mM sodium phosphate pH 7.0 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 mM 2-mercaptoethanol for 12 h and purified by anion exchange chromatography (HiTrap Q, GE Healthcare) with a linear gradient of 0 to 500 mM NaCl over 40 min and size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
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bioRxiv - Immunology 2022Quote: ... a Histrap excel 5 mL column (GE Healthcare) was used to isolate proteins ...
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bioRxiv - Microbiology 2020Quote: ... a HisTrap HP 5 ml column (GE Healthcare) was used ...
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bioRxiv - Microbiology 2020Quote: ... a MonoQ 5/50 GL column (GE Healthcare) was required to completely remove the GST-tag ...
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bioRxiv - Biochemistry 2020Quote: ... using a 5 mL HisTrap column (GE Healthcare). The bound protein was eluted using a linear imidazole gradient (0 - 0.5 M ...
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bioRxiv - Biophysics 2021Quote: ... while a Tricorn 5/20 column (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... a 5 ml HisTrap column (GE Healthcare, UK) was used for immobilized metal affinity chromatography (IMAC) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented 5% horse serum (GE Healthcare Life Sciences), 1% penicillin/streptomycin (Lonza) ...
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bioRxiv - Cell Biology 2022Quote: ... using a 5 mL StrepTrap HP (GE Healthcare). The washing buffer contained 100 mM Tris pH 8.0 and 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 5 ml HisTrap™ HP column (GE Healthcare). For further purification steps the column was connected to a liquid chromatography system (Azura ...
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bioRxiv - Molecular Biology 2020Quote: ... blocked with 5% membrane blocking agent (GE Healthcare) in TBS and incubated with the corresponding primary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... IMAC Column: 5 ml HisTrap HP (GE Healthcare); Gel Filtration Column ...
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bioRxiv - Molecular Biology 2022Quote: ... loaded onto 5 ml HisTrap column (GE Healthcare), equilibrated in buffer A ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 45 min at 37°C with shaking ...
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bioRxiv - Microbiology 2021Quote: ... A 5 ml HiTrap desalting column (GE Healthcare) was used to exchange the complex buffer into 100 mM ammonium acetate ...
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bioRxiv - Evolutionary Biology 2022Quote: ... supplemented with 5% fetal bovine serum (GE Healthcare) and 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Amersham 5 AI600 (GE Healthcare, USA).
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bioRxiv - Developmental Biology 2024Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in CO2 independent medium (Thermo Fisher Scientific)] for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... and imagen on a Typhoon 5 (GE Healthcare). DNA fragments were quantified using ImageJ (NIH ...