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Citations for GE Life Sciences :
901 - 950 of 2153 citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were loaded on a 5 ml HisTrap™HP column (1 mL/min; GE Healthcare) in a binding buffer containing 100 mM tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was applied to 1 ml Nickel resin (Ni Sepharose 6 Fast Flow, GE Healthcare) preequilibrated with 20 ml buffer A and 20 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v, GE Healthcare) and vimentin levels were estimated by western blot as described below ...
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bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with Anti-mouse HPR-conjugated secondary antibody (1:5000 dilution, GE Healthcare) at RT for 2 hours and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) was exchanged to a storage buffer omitting DTT using a PD10 (GE Healthcare) gel filtration column ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified cpxA31-163 was cloned into BamHI/EcoRI-digested pGEX-6P-1 expression vector (GE Healthcare), which encodes for an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded on a 1 ml HisTrap HP Q column (GE Healthcare, Pittsburgh, PA), and the flow-through was collected and dialyzed against 20 mM HEPES pH 6.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were probed with an ECL anti-rabbit IgG secondary antibody (1:10,000) from GE Healthcare Life Science and imaged using Chemidoc Molecular Imaging System (BioRad) ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM DTT) and applied to a Superdex 200 10/300 size exclusion column (GE Healthcare) that was pre-equilibrated with cross-linking buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Filtered lysates with strep-tagged proteins were applied onto StrepTrap HP 1 ml columns (GE Healthcare). Subsequent protein separation was conducted using the ÄKTA start chromatography system (GE ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred (100 V, 1 hr) onto PVDF (polyvinylidene difluoride) membranes (GE Healthcare Life Sciences) using 1X Tris/Glycine buffer (in-house 50X solution containing 250 mM Tris and 1920 mM Glycine) ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting clarified lysate was batch-bound to ~1 mL of settled IgG sepharose (GE Healthcare) equilibrated in lysis buffer and allowed to incubate on a roller for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Further purification method was carried out with a 1 mL HisTrap HP column (GE Healthcare, Sweden) to obtain the pure protein ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 2h at RT with streptavidin-HRP (1:400, GE Healthcare UK). After washing ...
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bioRxiv - Biochemistry 2022Quote: ... and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000; GE Healthcare; NA943V). Membrane was visualized using Radiance Plus chemiluminescent HRP substrate (Azure Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000; GE Healthcare; NA943V). Membrane was visualized as described above.
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bioRxiv - Cell Biology 2022Quote: ... and cloned into BamHI and NotI restriction sites within the pGEX-4T-1 plasmid (GE Healthcare), such that the gene was in frame with an N-terminal GST tag (pGEX-4T-1-GST-KIN-EC-ter) ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies: 1:10000 goat α rabbit or goat α mouse ECL (GE healthcare NA931, NA934).
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used: sheep anti-mouse (HRP-conjugated, GE Healthcare, NXA931V, 1:10000), donkey anti-rabbit (HRP-conjugated ...
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bioRxiv - Synthetic Biology 2023Quote: ... Recombinant proteins were then purified by 1 mL Ni Sepharose (17-5318-01, GE Healthcare, USA). Columns were sequentially washed with 20 mL buffer 1 and 10 mL buffer 2 (in mM ...
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bioRxiv - Biochemistry 2023Quote: ... The diluted sample was then applied to a 1 mL HiTrap Q HP column (GE Healthcare), and UBXD1 was eluted with a 0-50% gradient of AEX elution buffer (AEX binding buffer supplemented with 1000 mM KCl) ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtered (Filter paper, Grade 1; Size: 185 mm; Whatman, GE Healthcare Live Sciences), a 1 mL aliquot of the filtrate was transferred into a 1.5 mL centrifuge tube ...
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bioRxiv - Cell Biology 2024Quote: ... at 37 °C for 1 h and alkylated with 15 mM IAA (GE Healthcare, Mississauga, ON) in the dark at room temperature for 30 min followed by quenching with 15 mM DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies were used as follows: Horseradish peroxidase (HRP)-conjugated mouse IgG (1:10,000; GE Healthcare) or rabbit IgG (1:3000 ...
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bioRxiv - Biophysics 2024Quote: The adjusted pool was loaded onto a 1 ml Phenyl-HP hydrophobic interaction column (GE Healthcare) pre-equilibrated with PepA Buffer D then washed with the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... The crude PepA solution was loaded onto a HiTrap Heparin-HP 1 ml column (GE Healthcare), then washed with PepA Buffer A [PepA Buffer C without Glycerol] ...
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bioRxiv - Biophysics 2024Quote: ... then by a Heparin-affinity purification using a 1 ml HiTrap Heparin-HP column (GE Healthcare). Other FPLC steps from Subramanya et al ...
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bioRxiv - Biophysics 2024Quote: ... Free gold-cluster and unlabeled kinesin were removed through RESOURCE S column (1 ml, GE Healthcare) chromatography ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM DTT and 250 mM sucrose) using a PD-10 desalting column (GE Healthcare, 17085101) according to the user instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatant was mixed with 4 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer supplemented to 50 mM imidazole pH 8.0 and suspension was applied on Flex-Column (420400-2510 ...
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bioRxiv - Microbiology 2022Quote: ... rolling at 4°C with 100 μl of Ni-NTA Agarose (GE Healthcare) pre-equilibrated with the SEC buffer ...
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bioRxiv - Biochemistry 2021Quote: Purification was performed at 4°C using an ÄKTA™ pure (GE Healthcare) to control flow and column pressure ...
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bioRxiv - Microbiology 2020Quote: ... 100 µl of Glutathione Sepharose 4 Fast Flow (GE Healthcare, #GE17-5132-01) were packed onto Poly-Prep chromatography columns (Biorad ...
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bioRxiv - Molecular Biology 2022Quote: ... it was applied to a Con A-Sepharose 4 B column (GE Healthcare), and glycoproteins were eluted with 0.5 M α-D-methylglucoside ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysates were then incubated with Glutathione Sepharose 4 Fast Flow (GE Healthcare) to immobilize GST-fusion proteins.
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bioRxiv - Genetics 2020Quote: ... at 60 x magnification (Figs. 2 and 4) or DeltaVision microscope (GE Healthcare) at 100 x magnification (Fig ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 µL of Protein A Sepharose 4 FF (GE Healthcare Japan, Tokyo, Japan) was added and mixed with rotation at 4°C for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... were coupled overnight at 4°C to protein A Sepharose beads (GE Healthcare). Coupled beads were washed three times in EB buffer (50 mM Hepes ...
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bioRxiv - Biochemistry 2021Quote: ... The chromatography was undertaken at 4°C using an ÄKTA FPLC (GE Healthcare). The clarified supernatant was loaded onto a 5 mL prepacked Ni-nitrilotriacetic acid (NTA ...
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bioRxiv - Physiology 2023Quote: ... using a multifrequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare) was used to capture VL muscle cross-sectional area (mCSA) ...
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bioRxiv - Molecular Biology 2022Quote: ... overnight at 4°C in the presence of Protein A beads (GE Healthcare). ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... were applied along with Protein G Sepharose 4 Fast Flow beads (GE Healthcare). All ChIP extracts were pre-cleaned for 1 hr with 30 μl beads on a rotor at 4°C in absence of the antibody ...
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bioRxiv - Biochemistry 2023Quote: ... and Benzamidine Sepharose 4 Fast Flow were obtained from GE Healthcare (Uppsala, Sweden). HPE-IAM was obtained from Molecular Biosciences (Boulder ...
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bioRxiv - Physiology 2023Quote: ... The transfection mix consisted of 4 μL of DharmaFECT1™ (GE Healthcare, UK), 144 μL of 1 μM siRNA (35 nM final concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 mL of Amersham™ ECL Prime Western Blotting Detection Reagent (GE HealthCare) was added to each membrane in the dark for 1 min ...
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bioRxiv - Physiology 2023Quote: ... using a multifrequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare) was used to capture right and left leg images to determine VL muscle cross-sectional area (mCSA) ...
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bioRxiv - Biophysics 2022Quote: ... samples were loaded at 4 °C onto a Resource S column (GE Healthcare) equilibrated in 20 mM MES-NaOH pH 6.0 (for reactions containing FimCHisI or FimCHisIA) ...
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bioRxiv - Microbiology 2022Quote: ... the cell pellet was resuspended in 4 ml of 80% Percoll (GE Healthcare), added to a 15 ml falcon tube ...
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bioRxiv - Biochemistry 2023Quote: ... blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Runs were performed at 4°C through an ÄKTAmicro (GE Healthcare Life Sciences) system.