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Citations for GE Life Sciences :
851 - 900 of 3945 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 10 μL of IgG Sepharose (GE Healthcare, #17096901) pre-equilibrated with lysis buffer was added ...
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bioRxiv - Genomics 2024Quote: ... 10% fetal bovine serum (FBS, SH30066.03HI, GE Healthcare), and 0.01 mg/mL Hygromycin B (SH30066.03HI ...
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bioRxiv - Biophysics 2024Quote: ... and an S75 10/300 column (GE Healthcare) were performed as described in the GFP-tagged substrate purification section ...
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bioRxiv - Biochemistry 2024Quote: ... using Hiprep 23/10 desalting column (GE Healthcare). The purified proteins were taken for the size exclusion chromatography column (Superdex 75G 16/600 ...
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bioRxiv - Biophysics 2021Quote: ... and RBM3 (FliF231-438) were applied to a Superdex 200 10/300 column and to a Superose 6 10/300 column (GE Healthcare), respectively ...
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bioRxiv - Molecular Biology 2021Quote: All the gel filtration chromatograms were ran in a Superdex 200 increase 10/300 column (fractionation range 10-600 kDa, GE Healthcare) coupled to an AKTA purifier ...
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bioRxiv - Biochemistry 2020Quote: ... We purchased all columns (26/10 HiPrep [desalting], HisTrap HP [Ni], and HiPrep Q HP 16/10 [anion exchange]) from GE Healthcare, Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Nb was mixed with the target protein in 1.2-1.5 fold molar excess and the complex isolated via SEC on a Superdex 75 Increase 10/300 GL or Superdex 200 Increase 10/300 GL (GE healthcare) column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated in centrifugal concentrators and purified by size-exclusion chromatography (Superpose 6 In-crease 10/300 GL or Superdex 200 Increase 10/300 GL; GE Healthcare) in gel filtration buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... The relevant fractions were pooled and concentrated to 10-20 mg/mL for loading on a Superdex 200 Increase 10/300 (GE Healthcare) size exclusion chromatography column equilibrated with 30 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... Ni2+-NTA eluted fractions containing the polyprotein were concentrated to 10 mg/mL and further purified over a 10/300 Superdex 200 column (GE healthcare) in 20 mM phosphate buffer ...
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bioRxiv - Microbiology 2020Quote: ... the NP and 1TD0-derived epitope probes were further purified by SEC on a Superose 6 10/300 GL column and a Superdex 75 10/300 GL column (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2020Quote: Analytical gel filtration was performed on a Superdex 200 Increase 10/300 GL column or Superose 6 Increase 10/300 GL (GE Healthcare) pre-equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... was done by connecting of either a Superdex 200 Increase 10/300 or a Superdex 75 10/300 (both GE Healthcare) to a UV-Vis Prostar 335 detector (Varian ...
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bioRxiv - Biochemistry 2020Quote: ... and fl-SNED1 (4.5 μM) were injected on Superdex 75 Increase 10-300 GL and Superdex 200 Increase 10/300 GL columns (GE Healthcare) respectively at a flow rate of 0.5 ml/min at 20°C ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were subjected to size exclusion chromatography using either a Superdex 200 10/300 or Superdex 75 10/300 column attached to an AKTA Pure system (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... then UCN1-CRF2R-Go and UCN1-CRF2R-G11 were loaded onto a Superdex200 10/300 GL column and Superose6 Increase 10/300GL (GE Healthcare), respectively ...
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bioRxiv - Microbiology 2022Quote: ... A total of 100 μl containing 10 mg/ml of disassembled protein were loaded onto a Superdex 75 Increase 10/300 GL (GE Healthcare) using 300 mM NaCl for elution ...
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bioRxiv - Biophysics 2023Quote: ... and followed by size-exclusion chromatography (SEC) using a superdex 200 Increased 10/300 chromatography column on an AKTA Purifier 10 FPLC system (GE Healthcare) with buffer containing 20 mM Hepes ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then injected at a 0.4 mL/min flow rate onto a Superdex 200 GL 10/300 (or in the case of Figure S4, Superdex 200 Increase GL 10/300) SEC column (GE Healthcare) using HSEC buffer with 1 mM ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentrated protein sample was centrifuged at 10,000 × g and 4°C for 10 min before the supernatant was loaded onto a lysis buffer equilibrated Superose 6 increase 10/300 GL column (GE Healthcare), connected to a 1260 Bioinert Infinity LC system and equipped with a multi-angle light scattering detector (miniDawn 3141MD3 ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were eluted with HBS containing 200 mM imidazole and further purified using size-exclusion chromatography (Superdex 200 Increase 10/300 or Superose 6 Increase 10/300 columns; GE Healthcare) in 10 mM Tris ...
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bioRxiv - Immunology 2024Quote: ... Fab was buffer exchanged on a 10 kDa Amicon concentrator and purified through size exclusion chromatography with a Superdex 200 increase 10/300 column (GE Healthcare). Complexes were generated by incubating Fab and antigen overnight at a 50:1 ratio and purified through size exclusion chromatography with a Superdex 200 increase 10/300 column (GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: ... Further protein was centrifuged at 10000 x g for 10 min and subjected to gel filtration on Superdex 200 10/300 (GE Healthcare) equilibrated with GF buffer (20 mM HEPES pH 7.6 ...
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bioRxiv - Plant Biology 2024Quote: ... The dialysis product was subjected to another Ni-IMAC to remove the protease and the digested tags before the protein was purified in a final gel filtration step on a Superdex75 increase 10/300 GL column or a Superdex200 increase 10/300 GL column (GE Healthcare) equilibrated in gel filtration buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... The elutions were further purified by FPLC SEC using Superdex 75 10/300 GL or Superdex 10/300 200 columns (GE Healthcare). Protein concentrations were determined by NanoDrop (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Concentrated protein fractions were further purified by size exclusion chromatography (SEC) using a Superdex 75 Increase 10/300 GL column or a Superdex S200 10/300 GL column (GE Healthcare), depending on the molecular weight of the UPF2 variant ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples at concentrations >10 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2021Quote: ... The eluted protein was then buffer exchanged to 10 mM HEPES at pH 7.4 by PD-10 columns (GE Healthcare Life Sciences) following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... at a density of 0.3 x 106 cells/mL in 10 mL total volume/plate of complete culture medium (RPMI 1640 plus 10% heat-inactivated fetal bovine serum (FBS, GE Healthcare HyClone), 50 μM 2-mercaptoethanol ...
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bioRxiv - Genetics 2020Quote: ... the eluted protein solution was concentrated 10 times in a Vivaspin concentrator 30 kDa cut off and loaded in a Superose 6 increase 10/300 (GE Life Sciences) with PBS as a carrier buffer ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were concentrated with 30 kDa cutoff concentrators (Amicon) to ∼10 mg/ml and loaded onto a S200 Increase 10/300 GL (GE Life Sciences) in SEC Buffer [20mM Tris ...
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bioRxiv - Cell Biology 2024Quote: Purified LZTR1 proteins were centrifuged at 12,000×g for 10 min before being applied to an analytical Superose 6 10/300 SEC column (GE Healthcare Life Sciences) using a buffer containing 50 mM Tris/HCl (pH 7.4) ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% FBS (HyClone, GE Healthcare Life Science), penicillin (100 units/ml ...
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bioRxiv - Genetics 2021Quote: ... 10 ng (0.02 U) poly(dI-dC) (GE healthcare) and approximately 0.5 ng of 32P-labeled oligonucleotides were added ...
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bioRxiv - Molecular Biology 2021Quote: ... and Superdex 200 10/300 GL column (GE Healthcare) was equilibrated in 20 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 percent heat-inactivated FBS (GE Healthcare Bio-Sciences), and IL-2 at 5 ng/ml (PeproTech) ...
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bioRxiv - Neuroscience 2021Quote: ... and 10% fetal bovine serum (FBS, GE Healthcare Hyclone) for 3.5 hours allowing the dextran to be delivered to the lysosomes (chase pulse) ...
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bioRxiv - Neuroscience 2021Quote: ... and 10% fetal bovine serum (FBS, GE Healthcare Hyclone). Primary microglia were plated and allowed to rest 24 hours prior to the experiment ...
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bioRxiv - Molecular Biology 2021Quote: ... and a Superdex75 10/300 GL column (GE Healthcare) in buffer containing 30 mM HEPES pH 7.9 ...
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bioRxiv - Molecular Biology 2021Quote: ... and a Superdex75 10/300 GL column (GE Healthcare) in a buffer containing 30 mM HEPES pH 7.9 ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% bovine growth serum (GE Life Sciences), 10 units/mL penicillin ...
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bioRxiv - Neuroscience 2020Quote: ... and 10% fetal bovine serum (FBS, GE Healthcare Hyclone) to ensure the presence of the complement molecules ...
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bioRxiv - Cancer Biology 2020Quote: ... both supplemented with 10% bovine growth serum (GE Healthcare) and 1% penicillin-streptomycin (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... or a Superdex75 increase 10/300 column (GE Healthcare) using an AKTA pure (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... connected to an ÄKTA Purifier 10 system (GE Healthcare) and eluted with 1 CV ClpP-GF buffer ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% HyClone cosmic calf serum (GE Healthcare). Each transfection was with 15 µg total DNA at a 50%/37.5%/12.5% ratio of expression vector/pSPAX2/pMD2.G ...
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bioRxiv - Biochemistry 2021Quote: ... a Superdex 200 Increase 10/300 column (GE Healthcare) operated at a 0.8 ml/min flow rate was used (100 μl loading) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were applied to PD-10 columns (GE Healthcare) to remove residual Zn2+ ...
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bioRxiv - Biochemistry 2022Quote: Using an ÄKTApurifier UPC 10 FPLC system (GE Healthcare), lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection ...