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Citations for GE Life Sciences :
851 - 900 of 1068 citations for Sodium hexanoate 6 6 6 d3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... SDS-gels were stained by “Blue-Silver” Coomassie Staining (Candiano et al., 2004) and washed with water for 6 hours or overnight before imaging with LAS4000 (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained the protein were concentrated and further purified at 4 °C on a preparative Superose 6 XK 16/70 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... 500 µg clarified supernatant from P2 fractions was further fractionated by size-exclusion chromatography (SEC) on a Superose 6 increase column (GE Healthcare) equilibrated with LB buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... Samples were injected on the Äkta prime plus chromatography system (Cytivia) and separated on a Superose 6 10/300 column (GE Healthcare) in SEC buffer (10 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... and pellets were resuspended in 6 mL 25 % Standard Isotonic Percoll (Percoll: GE Healthcare, 17-5445-02 with 10% 10x PBS) in Medium A ...
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bioRxiv - Cell Biology 2023Quote: ... The GST-tagged Bridge domain was purified by affinity chromatography with glutathione-SepharoseTM 4B resins (Cytiva) followed by size exclusion chromatography with Superose 6 10/300 (GE Healthcare) in the running buffer containing 150 mM NaCl and 20 mM HEPES (pH 7.5).
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bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...
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bioRxiv - Biophysics 2023Quote: ... The mixed complexes were incubated over night at 4 °C and the formed R2SP complex was separated from excess SPAG1/PIH1D2 using a Superose 6 16/60 XK (GE Healthcare), see Supplementary Information for detailed protocol ...
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bioRxiv - Biophysics 2023Quote: ... the protein was diluted 10 times with PBS buffer and immobilized on a Nickel Sepharose 6 Fast Flow resin (GE Healthcare). The resin was washed extensively with milliQ water followed by PBS buffer pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentrated protein sample was centrifuged at 10,000 × g and 4°C for 10 min before the supernatant was loaded onto a lysis buffer equilibrated Superose 6 increase 10/300 GL column (GE Healthcare), connected to a 1260 Bioinert Infinity LC system and equipped with a multi-angle light scattering detector (miniDawn 3141MD3 ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer with 1 mM MgCl2 and 0.03% digitonin ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer with 0.03% digitonin ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated with a 100 kDa cutoff concentrator to 500 μL and loaded on a Superose 6 Increase 10/300 column (GE Healthcare) equilibrated with gel filtration buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... the soluble fraction was passed over the appropriate affinity resin of Strep-Tactin XT Superflow (IBA) or Ni Sepharose 6 Fast Flow affinity resin (GE Healthcare), eluted with wash buffer (50 mM Tris–HCl pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: Size exclusion chromatography (SEC) was performed using ÄKTAprocess 6 mm Std system controlled by the Unicorn 7.0 software (GE Healthcare, Sweden). Sepharose 6 Fast Flow beads (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Cellular lysates were separated on a Superose 6 HR 10/30 size-exclusion column and an AKTA Purifier protein purification system (GE Healthcare), essentially as described previously(61) ...
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bioRxiv - Biochemistry 2022Quote: ... The ACE2-induced postfusion S-nanodisc sample was purified by gel filtration chromatography on Superose 6 Increase 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... The concentrated protein sample was further purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL (GE Healthcare) column pre-equilibrated with the buffer E (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... After His-tag purification protein was subsequently purified by size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare). The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were eluted with HBS containing 200 mM imidazole and further purified using size-exclusion chromatography (Superdex 200 Increase 10/300 or Superose 6 Increase 10/300 columns; GE Healthcare) in 10 mM Tris ...
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bioRxiv - Plant Biology 2023Quote: ... for 2 hours and then extensively washed with lysis buffer and eluted in lysis buffer with 5mM desthiobiotin and further purified by size-exclusion chromatography with Superose 6 increase 10/300 GL-column (GE Healthcare).
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bioRxiv - Immunology 2023Quote: ... Plasma lipoproteins were separated using fast-performance liquid chromatography with two Superose 6 10/300 GL columns (GE Healthcare; Boston, MA) on a Shimadzu HPLC system (Columbia ...
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bioRxiv - Immunology 2023Quote: ... Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare) in TBS
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bioRxiv - Immunology 2023Quote: ... protein was purified using cell-free culture supernatant by StrepTactin resin (IBA) and by size-exclusion chromatography using Superdex 200 (RBD and NTD) or Superose 6 (S-2P) column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of 100% ethanol or 10% DEET in ethanol was applied to a 2 cm x 6 cm strip of Whatman filter paper (GE Healthcare). The treated paper was hung from a rectangular plastic frame in front of the Peltier element ...
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bioRxiv - Cell Biology 2023Quote: ... Elution was performed with imidazole and the second step of purification was performed using Superose 6 Increase 10/300 GL gel filtration chromatography (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The harvested cells were lysed by pulsed sonication and the soluble fraction was affinity purified using Ni2+-Sepharose (Ni Sepharose 6 Fast Flow, GE Healthcare). The resulting MsLadC variants ...
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bioRxiv - Biophysics 2023Quote: ... The 6×His-tagged SUMO fusion proteins were purified using a HisTrap™ HP Ni2+ affinity column (GE Healthcare Life Sciences). The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... The eluted sample was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and purified by Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was treated with 100 mM phosphate buffer pH 7.5 and 50 mM EDTA before purification by gel filtration at room temperature on a Superose-6 Increase column (GE Healthcare) equilibrated with buffer containing 25 mM TRIS pH 8 ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superose 6 Increase size exclusion chromatography column (10/300 prep grade, GE Healthcare). Elution was carried out with 150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... dIgA-604 and SIgA-604 were purified using Ni-NTA beads (Smart-Lifesciences) and a Superose 6 Increase column (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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bioRxiv - Immunology 2023Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was concentrated to 100 µL using a 100 kDa MWCO concentrator and the concentrate was loaded onto a size-exclusion chromatography (SEC) column (Superose 6 Increase 10/30 GL, GE Healthcare), equilibrated in a buffer composed of 20 mM Tris-Cl (pH 8) ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM TCEP) and incubated for 1 hour at 4°C before loading on Superose 6 5/150 column (GE Healthcare) equilibrated with the same buffer on an AKTA-micro system (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The fractions containing L–P protein were further purified using a size-exclusion column (Superose 6 Increase 10/300 GL, GE healthcare) equilibrated with size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2024Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was desalted by a Zeba spin desalting column (7 kDa MWCO) and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare). Fluorescein- labeling efficiency was analyzed by SDS-PAGE and fluorescence gel imaging (Amersham Typhoon FLA 9500 ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated using a 100 kDa filter to approximately 1 mL and further purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer containing 0.06% digitonin ...
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bioRxiv - Immunology 2024Quote: ... Fab was purified from clarified culture supernatant six days post-transfection using Ni-NTA affinity chromatography with IMAC Sepharose 6 Fast Flow resin (GE Healthcare). Gel filtration on a Superdex 75 10/300 column (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was desalted by a Zeba spin desalting column (7 kDa MWCO) and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare). Fluorescein-labeling efficiency was analyzed by SDS-PAGE and fluorescence gel imaging (Amersham Typhoon FLA 9500 ...
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bioRxiv - Microbiology 2024Quote: ... with 100 kDa molecular weight cut-off and subsequently injected into a Superose 6 increase 10/300 GL size exclusion column (GE Healthcare) pre-equilibrated in SEC buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... Variants were purified from filtered supernatant via gravity flow over StrepTactin resin (IBA) followed by gel-filtration chromatography on a Superose 6 10/300 column (GE Healthcare) into a buffer consisting of 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... blood was collected via retro-orbital bleeding from adult C57BL/6N mice (6 weeks old) and PBMCs were harvested using Ficoll-Plaque PLUS (GE Healthcare). T cells ...
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bioRxiv - Microbiology 2024Quote: ... Extension cDNA products were analyzed on 6% acrylamide 8[M urea-sequencing gels and visualized by Typhoon FLA 7000 phosphorimager (GE Healthcare). The four samples of the Sanger sequencing for dnaN were loaded to the same gel and served as a reference.
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bioRxiv - Molecular Biology 2024Quote: ... Reaction products were run on a 6% non-denaturing polyacrylamide gels and quantified using a Typhoon FLA 7000 phosphorimager (GE Healthcare) and ImageJ software.
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bioRxiv - Molecular Biology 2024Quote: ... 140∼150 uL of 6∼7 mg/mL of each complex solution was injected into the superdex200 increase 10/300 (GE Healthcare) column ...
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bioRxiv - Molecular Biology 2024Quote: PCR products were resolved on a 6% non-denaturing polyacrylamide gel and quantified using a Typhoon FLA 7000 phosphorimager (GE Healthcare). The percent of the NLRP3 mRNA including the targeted exon was calculated relative to the abundance of the predominant ASO-induced isoform after the signal was corrected for the number of cytosine nucleotides ...