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Citations for GE Life Sciences :
851 - 900 of 1616 citations for Hydrotris pyrazol 1 ylborato trichlorotitanium IV since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the lysate was loaded onto a 1 ml StrepTrap HP column (GE Healthcare Life Sciences, Buckinghamshire, UK) that was equilibrated with Lysis buffer.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were detected using either HRP-linked donkey anti–rabbit IgG (GE Healthcare, #NA934V; 1:10,000) or HRP-linked sheep anti– mouse IgG (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... were used together with horseradish peroxidase-conjugated sheep α-mouse immunoglobulin G (dilution: 1:2,000) (GE Healthcare) as a secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) and separated at 0.25 ml min-1 using an AKTA Pure instrument (GE Healthcare). One ml fractions were collected ...
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bioRxiv - Molecular Biology 2022Quote: ... GST tagged Mcl-1 was purified using GST-affinity chromatography (Glutathione-Sepharose resin by GE Healthcare, Fastflow). Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was added to equilibrated glutathione-Sepharose 4B beads (GE Healthcare; 1 ml/100 g cells) and gently rotated for 4 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... were used together with horseradish peroxidase-conjugated sheep α-mouse immunoglobulin G (dilution: 1:2000) (GE Healthcare) as a secondary antibody ...
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT and further purified by ionic exchange chromatography using a Mono-Q column (GE Healthcare). Protein was eluted with a linear gradient of a buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... The sample was loaded onto an immobilized metal-affinity column (1 mL, HIStrap HP column, GE Healthcare), which was washed with 50 mM sodium phosphate (NaPi ...
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bioRxiv - Microbiology 2022Quote: ... Eggs were collected on a wet filter-paper (Grade 1 filter paper, Whatman plc, GE healthcare, UK), desiccated for 5 days and later hatched in deionized water containing 1g/L bovine liver powder (MP Biomedicals ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes incubated with an anti-mouse HRP-linked secondary antibody (1:5000, GE Healthcare, NXA931, RRID:AB_772209). Either Pierce ECL Western Blotting Substrate (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were incubated with horseradish peroxidase (HRP)-coupled sheep anti-mouse IgG (GE Healthcare, NA931VS; 1/10000) and (HRP)-coupled goat anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Genetics 2024Quote: ... Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse (1:5000; NA934 and NA931 respectively, GE Healthcare) were used as secondary antibodies ...
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bioRxiv - Biophysics 2024Quote: ... FPLC was performed by loading the lysate supernatant onto a 1 ml MBP-Trap column (GE Healthcare), followed by a wash step using the same lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 min and imaged using the chemiluminescence setting on an Amersham 680 gel imager (GE Healthcare). Blots were adjusted for brightness and contrast using GIMP (GNU Project ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated with anti-mouse IgG-HRP (1:5000 dilution, GE Healthcare, cat no. NA931) for 1 hr at RT ...
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bioRxiv - Biophysics 2024Quote: ... The soluble supernatant was incubated with 1 ml glutathione sepharose 4B resin (GE Healthcare, 17-0756-01) for 2.5 hours at 4 °C with rolling ...
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bioRxiv - Physiology 2024Quote: ... The secondary antibody was used a horseradish peroxidase-conjugated donkey anti-rabbit antibody (1:2000) (GE Healthcare). Immunoreactive bands were visualized using an enhanced chemiluminescence detection system as previously described31 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Mixtures were separated in 1×TBS+ through a Superdex 200 Increase 10/300 GL (GE Healthcare, 28990944) column ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using an AKTA FPLC with 1 mL nickel-charged HisTrap columns (GE Healthcare Life Sciences) for affinity chromatography of the His-tagged proteins ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT at a flow rate of 2 ml/min using an Akta purifier system (GE Healthcare), eluting at approximately 100 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies were incubated for 1 hour at RT and signals were detected with ECL Select (GE Healthcare) and Odyssey Fc (Li-Cor) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... it was passed through Whatman™ Grade 1 filter paper (diameter 150 mm) (GE Healthcare Life Sciences, Ireland), followed by 0.45-μm membrane filtration and then 0.22-μm membrane filtration (ThermoFisher Scientific Ltd) ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was probed with HRP-conjugated anti-mouse or anti-rabbit (1:5,000, GE Healthcare Bio-Sciences) secondary antibody after washing several times with 1X PBS for 45 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The amplified wild type and mutant PDE12 cDNAs were cloned into plasmid pGEX-6P-1 (GE Healthcare, USA) at the EcoRI and SalI restriction site ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatants were collected by centrifugation and purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... GST-TEV-FICD was then depleted by a 1 h incubation with GSH-Sepharose 4B matrix (GE Healthcare). AMPylation was confirmed as being stoichiometric by intact-protein mass spectrometry (LC-ESI-MS ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μL of plasma was loaded onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, USA) and left to dry out ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was transferred on a column containing 1 ml Ni-Sepharose High Performance resin (GE Healthcare), washed two times with lysis buffer containing 20 mM and 40 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT with USP7 proteins purified as described previously 62 using Glutathione Sepharose 4B beads (GE Healthcare). After elution the GST tag was removed under dialysis using 3C protease and the sample fractionated by PorosXQ anion exchange (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Microbiology 2021Quote: ... The lysate supernatant was than loaded on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) pre-equilibrated with a binding buffer (8 M Urea ...
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bioRxiv - Biophysics 2020Quote: ... excess FluoZin-1 dye was removed by passing the sample through a PD-10 desalting column (GE Healthcare) pre-equilibrated with reconstitution buffer.
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bioRxiv - Biophysics 2021Quote: ... the sample was loaded at 2 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies were diluted in blocking solution at 1:1000 (Anti-rabbit IgG: GE Healthcare Cat#NA934, RRID:AB_772206) and 1:2000 (Anti-mouse IgG ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D534 was also purified using 1 mL of M2 anti-FLAG affinity column (GE healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: The HMWP2-Cy2 supernatant was loaded at 1 ml/ min onto a 5 mL HisTrap column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Filtered lysate was loaded onto a pre-equilibrated 1 mL or 5 mL HisTrap HP column (GE Healthcare) charged with Ni2+ ...
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bioRxiv - Cell Biology 2022Quote: ... followed by anti-rabbit-IgG and mouse-IgG antibodies coupled to HRP (1:2000, NA934, NA931, GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... DNA fragments encoding APC proteins were inserted into pGEX-5X-1 (GE Healthcare Life Sciences, Pittsburgh, PA, USA). For in vitro transcription and translation reaction ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with TBST containing horseradish peroxidase-conjugated secondary antibody (1:1000; GE Healthcare, Pittsburgh, PA) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... the membrane was incubated for 1 hour with horseradish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare, Piscataway, NJ). The target protein on the membrane was detected by Pierce™ ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) and then loaded on a HiTrap Heparin column (CV, column volume 5 ml; GE Healthcare), equilibrated with buffer A ...
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bioRxiv - Neuroscience 2020Quote: ... monkey (XP_005545315.1; 121-760aa) and human (NP_001121620.1; 120-760aa) TfR1 in 0.1% BSA in HBS-EP+ buffer (GE Healthcare) was captured at flow rate 10 μl/min ...
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bioRxiv - Synthetic Biology 2020Quote: The DNA fragment encoding the PreS1 domain (residues 1-56) was cloned into the pGEX-2T (GE Healthcare) plasmid ...