Labshake search
Citations for GE Life Sciences :
851 - 900 of 6111 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... the protein was eluted using an AKTA FPLC (GE healthcare) and again checked for purity using SDS-PAGE.
-
bioRxiv - Cell Biology 2024Quote: ... Antigenic proteins were coupled to NHS-activated sepharose (GE Healthcare), whereas antigenic peptides were coupled to SulfoLink coupling gel (ThermoFisher) ...
-
bioRxiv - Molecular Biology 2024Quote: ... proteins were electroblotted to PVDF membrane (0.2 μM, GE Healthcare) for 1 h at 100 V in ice-cold Bolt™Transfer Buffer (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2024Quote: ... Biotinylated proteins were captured with Streptavidin Sepharose beads (GE Healthcare) and bound proteins analyzed by mass spectrometry.
-
bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Glutathione Sepharose 4B (GE Healthcare). GST was cleaved from protein by TEV protease treatment for 16 hours at 4 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... The labeled proteins were purified with a PD10 (GE Healthcare) gel filtration column pre- equilibrated with Storage buffers (hSSB1 ...
-
bioRxiv - Biochemistry 2024Quote: ... The labeled protein was purified with a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer and dialyzed against storage buffer to remove residual free dye.
-
bioRxiv - Biochemistry 2024Quote: ... the labeled protein was purified using a PD10 (GE Healthcare) gel filtration column pre- equilibrated with INIP Storage buffer (25 mM Tris-HCl pH 8 ...
-
bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred to nitrocellulose membranes (GE Healthcare). For immunoprecipitations ...
-
bioRxiv - Cell Biology 2024Quote: ... samples were pre-cleared with Protein G Sepharose (GE Healthcare) for 1h and subsequently incubated with 1-2 μg of antibodies for 2h at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
-
bioRxiv - Microbiology 2019Quote: ... 4°C and applied to a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 ml/min ...
-
bioRxiv - Biochemistry 2019Quote: ... 4°C and loaded on a HisTrap column (GE Healthcare, Chicago, Illinois, United States) for purification ...
-
bioRxiv - Microbiology 2019Quote: ... 4°C and applied to a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 ml/min ...
-
bioRxiv - Biochemistry 2019Quote: Size-exclusion chromatography was performed at 4 °C on an AKTA FPLC (GE Healthcare). Approximately 10 mg of apoferritin (Sigma A3660-1VL ...
-
bioRxiv - Biochemistry 2020Quote: Binding studies were performed at 25°C using a MicroCal iTC200 instrument (GE Healthcare). ANT(9 ...
-
bioRxiv - Plant Biology 2021Quote: SPR experiments were performed at 25°C on a Biacore T200 apparatus (GE Healthcare) in running buffer (20 mM Tris ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were filtered (1.2 μm, GF/C, Whatman, GE Healthcare, Little Chalfont, United Kingdom) and the flow-through processed for exometabolomic analysis as described above (see co-culturing experiment) ...
-
bioRxiv - Biochemistry 2022Quote: ITC was performed at 25°C using a VP-ITC MicroCalorimeter (MicroCal, GE Healthcare). CrRabL2 and CrCEP19 were buffered in 10mM Hepes pH 7.5 ...
-
bioRxiv - Cell Biology 2022Quote: Microscopy was performed at 30°C on a Delta Vision Deconvolution Microscope (Applied Precision), using InsightSSITM Solid State Illumination of 435 and 525 nm ...
-
bioRxiv - Microbiology 2020Quote: ITC measurements were carried out at 25 °C on a MicroCal iTC200 (GE Healthcare). For the titration of an inhibitor to DHODH ...
-
bioRxiv - Microbiology 2021Quote: ... the culture was applied to a CM Sephadex™ C-25 column (GE Healthcare) equilibrated with MQ ...
-
bioRxiv - Biochemistry 2021Quote: SPR analyses were performed at 25°C in a BIAcore T200 instrument (GE Healthcare). Highly purified ...
-
An apical protein, Pcr2, is required for persistent movement by the human parasite Toxoplasma gondiibioRxiv - Cell Biology 2022Quote: ... image stacks were acquired at 37°C using a DeltaVision imaging station (Applied Precision) with a COOLSNAPHQ2 camera (Photometrics®) ...
-
bioRxiv - Immunology 2022Quote: ... 0.005% v/v Surfactant P20 at 25 °C using the Biacore 8K (GE Healthcare), with a series S protein A sensor chip (GE Healthcare) ...
-
bioRxiv - Neuroscience 2023Quote: ... with MOPS buffer and transferred overnight at 4°C onto nitrocellulose membranes (GE Healthcare) with Tris-Glycine transfer buffer (25 mM Tris-base ...
-
bioRxiv - Immunology 2023Quote: SPR experiments were conducted at 25°C on a Biacore T200 instrument (GE Healthcare) using 10 mM HEPES-HCl (pH 7.4) ...
-
bioRxiv - Biochemistry 2023Quote: ... SPR measurements were performed at 25 °C using a Biacore T200 apparatus (GE Healthcare) and 50 mM HEPES-NaOH pH 7.4 ...
-
bioRxiv - Biochemistry 2023Quote: ... cation exchange gel and SP Sephadex C-25 (GE Healthcare Bio-Sciences AB, Sweden) strong cation exchange particles ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated overnight at 4 °C with anti-GST antibody (GE Healthcare) at a 1/1000 dilution ...
-
Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and cleaved from GST resin overnight at 4°C with PreScission Protease (GE Healthcare). GST was affinity purified as described above and eluted using 15 mM reduced L-gluathione ...
-
bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
-
bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
-
bioRxiv - Cell Biology 2020Quote: ... using siRNA SMARTpool ON-TARGETplus Human UBTD1 (80019) or ON-TARGETplus Non-Targeting Control siRNAs) (GE Healthcare). Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668 ...
-
bioRxiv - Biochemistry 2022Quote: ... and human FANCD2 was further purified by gel filtration chromatography on a Superdex 200 column (GE Healthcare) equilibrated with Buffer B containing 200 mM NaCl ...
-
bioRxiv - Immunology 2020Quote: ... Human PBMCs were isolated from the blood by density gradient using Percoll (GE Healthcare, 17-5445-01). PBMCs were washed and resuspended in RPMI 1640 supplemented with 10% FBS.
-
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
-
bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare, Cat#17-5442-03). B cells were purified by negative selection (Miltenyi Biotec ...
-
bioRxiv - Immunology 2024Quote: Human PBMCs were obtained from buffy coat by density gradient centrifugation in Ficoll–Paque PLUS (GE Healthcare). PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec ...
-
bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (GE healthcare). The membrane was blocked with 5% non-fat dry milk in PBS containing 0.1% v/v Tween 20 (PBST ...
-
bioRxiv - Cancer Biology 2021Quote: ... GST fusion proteins were bound to glutathione-Sepharose beads (GE Healthcare) by rotating in binding buffer (50 mM Tris HCl pH 8.0 ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were electrotransferred onto a 0.2 μm nitrocelluose membrane (GE Healthcare) and blocked with 5 % skim milk in 0.1 % Triton in Tris Buffer Saline (T-TBS ...
-
bioRxiv - Cell Biology 2020Quote: ... For western blotting proteins were transferred on nitrocellulose membrane (GE Healthcare).
-
bioRxiv - Cell Biology 2020Quote: ... Protein input was detected using an anti-GST antibody (GE Healthcare).
-
bioRxiv - Immunology 2021Quote: ... Secreted mAbs were purified using a Protein G column (GE Healthcare) and concentrated for use in downstream assays.
-
bioRxiv - Microbiology 2021Quote: ... The labelled proteins were then visualized using ECL prime (GE Healthcare) and LAS3000 (GE Healthcare) ...
-
bioRxiv - Microbiology 2019Quote: ... Proteins were then transferred to Hybond-P PVDF membrane (GE Healthcare). Immuno-staining was performed with 1/100 anti-MYD88 antibody (ab2064 ...
-
TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred and immobilized onto a nitrocellulose membrane (GE Healthcare) by electrophoresis for 2 h at RT in a standard transfer buffer containing 20% methanol ...