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Citations for GE Life Sciences :
851 - 900 of 2760 citations for Ethyl 5 3R 3 4 dihydroxybutyl thiophene 2 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... each AP2 domain was previously cloned into the pGEX-4-T1 vector (GE Life Sciences) and transformed into BL21- CodonPlus(DE3)-RIL E ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified from clarified supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads followed by a HiLoad 16/60 Superdex 75 size exclusion chromatography.
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bioRxiv - Cell Biology 2024Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) for 4h at 4°C with rotation ...
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bioRxiv - Immunology 2024Quote: ... for 30 min at 4°C and with Streptavidin Sepharose High Performance beads (GE Healthcare) for 2h at 4°C ...
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bioRxiv - Genetics 2020Quote: ... Eluted fractions were diluted 3-fold with R buffer and loaded onto a HiTrap SP column (GE Healthcare). Rrp1 eluted at around 600 mM NaCl with a linear gradient of 0.1-1.0 M NaCl in R buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was transferred on a column containing 3 ml Ni-Sepharose High Performance resin (GE Healthcare), washed two times with lysis buffer containing 30 mM and 50 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... zGDNFmat -zGFRα1D1-3 was further purified using size exclusion chromatography using a Superdex 200 (16/600) (GE Healthcare) in 20 mM Tris (pH 7.0) ...
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bioRxiv - Immunology 2020Quote: ... separated by SDS-PAGE using a 3-15% Tris-Acetate gel and transferred to nitrocellulose membranes (GE Healthcare). Densitometry analysis was performed by the Image J software (National Institutes of Health).
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bioRxiv - Immunology 2020Quote: Buffy coats were diluted [1:3] with PBS and added to Leukosep tubes containing Ficoll-Paque (GE Healthcare). Tubes were centrifuged at 450g for 40 minutes and the resulting buffy layer extracted ...
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bioRxiv - Neuroscience 2020Quote: ... age range: 20-29 years) were scanned in a 3-Tesla MRI scanner (DISCOVERY MR750, GE Healthcare, USA) using a 32-channel head-coil ...
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bioRxiv - Immunology 2020Quote: Whole-blood leukocyte DNA was extracted using the Nucleon BACC 3 DNA extraction kit (GE healthcare, Buckinghamshire, UK). We genotyped patients using the Illumina OmniExpressExome-8 Bead Chip (Illumina ...
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bioRxiv - Genomics 2022Quote: ... with an anti-rabbit IgG (goat polyclonal, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4301, RRID:AB_2650489) secondary antibody was used as a loading control ...
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bioRxiv - Neuroscience 2023Quote: ... MRI images were acquired with the aid of a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI) and an 8-channel head coil ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Membranes were washed with PBS-T for 3 × 10 min and were developed by ECL Prime (GE Healthcare) and imaged on a Biorad ChemiDoc MP Imaging System.
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bioRxiv - Immunology 2024Quote: ... The peak of properly refolded HLA-G2 was collected and buffer exchanged into HBS-EP (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA and 0.005% [v/v] Surfactant P20, GE Healthcare) for surface plasmon resonance (SPR ...
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bioRxiv - Biochemistry 2024Quote: ... Active fractions were pooled and concentrated using a 3-kDa molecular weight cutoff filter (Vivaspin 500, GE Healthcare), and used for further assays.
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bioRxiv - Microbiology 2022Quote: ... and 1 mM 2-mercaptoethanol and refolded on a NiFF (GE Healthcare) column using a reverse linear urea gradient with imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM dithiothreitol) and loaded onto Superdex S200 column (GE Healthcare) The column was equilibrated and the protein was eluted with buffer C (20 mM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... and subcloned into the pGEX 4T-2 vector (GE Healthcare Life Sciences) for expression in BL21 bacteria ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.2 using a 2×5ml HiTrap desalting column (GE Life Sciences) and concentrated to 10 mg/mL in a centrifugal concentrator ...
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bioRxiv - Biophysics 2022Quote: ... the supernatants were incubated with 2 mL glutathione-Sepharose beads (GE Healthcare) at 4°C for 3 hrs ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT) was passed through HisTrapTM FF (GE Healthcare Life Sciences) column ...
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bioRxiv - Microbiology 2020Quote: ... with a Ni2+ HiTrap IMAC (2 × 1 ml) column (GE Healthcare, Poland) preequilibrated with the binding buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and applied to tandem fast-flow Ni+2-NTA HisTrap (GE Healthcare) columns ...
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bioRxiv - Immunology 2022Quote: ... 2 mM CaCl2 using a HiPrep 26/10 Desalting column (GE Healthcare) and subsequently treated with enterokinase overnight at room temperature to remove the strep-tag ...
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bioRxiv - Microbiology 2021Quote: ... NEC220 was passed over 2 × 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated with 2 mL Ni-NTA resin (GE Healthcare), equilibrated with buffer A (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... RMI1/2 was further purified using a Superdex 200 Increase (GE Healthcare) in buffer I ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM TCEP) and applied to a 25 ml Source15Q (GE Healthcare) strong anion exchange column equilibrated in buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... Images for Figure 2 were obtained using a DeltaVision Elite (Applied Precision) maintained at 37°C and 5% CO2 equipped with a 40x and 63x PLANApo S lens (Olympus ...
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bioRxiv - Immunology 2023Quote: ... C4BPα1-2 was applied to a HiTrap Q HP column (GE Healthcare) and eluted using a 0-1 M NaCl gradient in 50 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were loaded on a 2 ml HisTrap beads column (GE Healthcare) functionalized with Nickel and equilibrated with a Buffer E ...
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bioRxiv - Biophysics 2021Quote: ... and the supernatant was loaded onto a 5 ml HisTrap HP column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... backside blotted for 5-10 s with Whatman filter paper grade 1 (GE Healthcare) and vitrified in liquid ethane using a home-made manual plunger.
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysate was loaded onto a 5 mL HisTrap sepharose HP column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Neuroscience 2021Quote: ... placed in 5% milk PBST plus 1:2500 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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Synaptic Zn2+ potentiates the effects of cocaine on striatal dopamine neurotransmission and behaviorbioRxiv - Neuroscience 2021Quote: ... for 5-days and imaged using a phosphor imager (Typhoon FLA 7000; GE Healthcare). Sections were analyzed using Multigauge software (Fujifilm ...
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bioRxiv - Biophysics 2022Quote: ... the solution was then loaded onto HiTrap HP 5 ml affinity column (GE Healthcare) pre-equilibrated with Buffer A ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was directly loaded onto HiTrap HP 5 ml affinity column (GE Healthcare) pre-equilibrated with Buffer A (50 mM Tris.HCl pH 8 ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...
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bioRxiv - Biochemistry 2020Quote: ... the filtered medium was loaded on a 5 mL HisTrap Excel column (GE Healthcare) equilibrated with buffer AG.A (500mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysate was loaded onto a 5 ml HisTrap FF Crude column (GE Healthcare), washed in a buffer containing 20 mM HEPES-KOH ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysate was loaded onto a Strep-Trap HP column (5 mL, GE Healthcare), equilibrated according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied to a pre-equilibrated 5 ml HisTrap column (GE Healthcare) using a peristaltic pump ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT) and loaded onto a MonoQ 5/50 GL column (GE Healthcare) equilibrated with MonoQ buffer A containing 50 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was loaded onto a 5 ml HisTrap FF Crude column (GE Healthcare) equilibrated with wash buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The eluent was loaded onto a 5 mL StrepTrap™ HP column (GE Healthcare) equilibrated in gel filtration buffer (20mM Tris pH 8.5 ...